AT1G09400

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000785
Physical Location & Seq
Reverse (-)
3033512 .. 3035396
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09400.2

Sequence Viewer

Length: 975 bp
ATGAAAAACTTCAATCTTACTCACAGAATCGTTATGGCGCCAATGGCGAGAATGAGATCATACGGTAACATTCCACAACCTCACGTCGCCTTGTATTATTGTCAAAGAACAACTCCGGGAGGTCTTCTCATCAGTGAAGCCACCGGAGTTTCTGAAACCGCCATGGCGTATCAAAACATGCCAGGGATATGGAGGAAAGAACAAATCGAAGCATGGAAGCCCATCGTTGACGCTGTTCACTCTCACGGCGGCATCTTCTTCTGCCAGCTCTGGCACGCTGGTCGCGTTTCTCATCAAGATTGTCAGCCAAATGGAGAATCTCCGGTCTCTTCAACAGATAAGCCATTCGCCGATGATCCATCGAATGAGTTTACACCTCCGAGAAGGTTAAGAACTGACGAAATCCCGACAATTATCAATGATTTTCGTCTCGCTGCAAGAAACGCAACCGAAGCTGGTTTTGATGGAGTAGAGATCCATGGAGCTCACGGGTACTTGATTGACCAGTTTATGAAAGACTCTGTTAACGATCGAACCGATAGTTACGGCGGATCTCTAGAGAATCGATGTAGATTCGCTTTACAAGTAATCGAAGCGGTTTCGAAAGAGATCGGACCTGATCGTGTCGGAATCAGACTCTCACCGTTCGCTGATTACATGGAATCCGGAGATACAGATCCAAAAAGATTAGGGCTTTACATGGCCAAGTCTCTCAACAGATTCGAGATACTCTACTGCCACATGATTGAGCCTAGGATGAAAACCGTCAGTGAGATTTTCGAGTGCAGGGAATCACTTACGCCGATGAGAAATGCGTTTAATGGAACGTTCATTGTCGCCGGAGGTTATACAAGAGAAGACGGAAACAAGGCGGTGGCAGAGGGAAGAACAGATCTTGTAGCGTACGGAAGATTGTTCTTAGCTAATCCTGATTTGCCTAAAAGGTTTGAGCTCAATGCTCCGCTGAATAAGTAA

Protein Analysis

324

Amino Acids

36.41

Weight (kDa)

6.32

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 2 - 263 1.1e-69 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AccII CGCG 1 cut(s) 285
AccIII TCCGGA 1 cut(s) 665
AciI CCGC 6 cut(s) 159, 249, 549, 596, 872, 962
AclI AACGTT 1 cut(s) 827
AclWI GGATC 4 cut(s) 350, 469, 559, 671
AcoI YGGCCR 1 cut(s) 702
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 2 cut(s) 494, 905
AgsI TTSAA 2 cut(s) 13, 333
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 5 cut(s) 268, 455, 485, 923, 952
AluI AGCT 5 cut(s) 268, 455, 485, 923, 952
Alw21I GWGCWC 2 cut(s) 487, 954
Alw26I GTCTC 3 cut(s) 331, 434, 714
AlwI GGATC 4 cut(s) 350, 469, 559, 671
Aor13HI TCCGGA 1 cut(s) 665
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 8
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 614
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 633
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 1 cut(s) 614
AvrII CCTAGG 1 cut(s) 752
BalI TGGCCA 1 cut(s) 704
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 2 cut(s) 487, 954
BbsI GAAGAC 2 cut(s) 116, 864
Bbv12I GWGCWC 2 cut(s) 487, 954
BbvI GCAGC 1 cut(s) 421
BccI CCATC 3 cut(s) 230, 367, 458
BceAI ACGGC 2 cut(s) 262, 562
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 183
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 3 cut(s) 331, 434, 714
BfaI CTAG 2 cut(s) 557, 753
BfoI RGCGCY 1 cut(s) 41
BglII AGATCT 1 cut(s) 892
BisI GCNGC 2 cut(s) 250, 435
BlnI CCTAGG 1 cut(s) 752
BlsI GCNGC 2 cut(s) 251, 436
Bme1390I CCNGG 2 cut(s) 117, 183
Bme18I GGWCC 1 cut(s) 614
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 117, 183
BmsI GCATC 1 cut(s) 261
BpiI GAAGAC 2 cut(s) 116, 864
BplI GAGNNNNNCTC 2 cut(s) 111, 143
Bpu14I TTCGAA 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 565
BsaBI GATNNNNATC 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 331
BsaJI CCNNGG 4 cut(s) 162, 182, 478, 752
BsaWI WCCGGW 3 cut(s) 143, 322, 665
Bse1I ACTGG 1 cut(s) 505
Bse8I GATNNNNATC 1 cut(s) 675
BseAI TCCGGA 1 cut(s) 665
BseBI CCWGG 1 cut(s) 183
BseCI ATCGAT 1 cut(s) 565
BseDI CCNNGG 4 cut(s) 162, 182, 478, 752
BseGI GGATG 1 cut(s) 762
BseJI GATNNNNATC 1 cut(s) 675
BseNI ACTGG 1 cut(s) 505
BseXI GCAGC 1 cut(s) 421
BsgI GTGCAG 1 cut(s) 805
Bsh1236I CGCG 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 532
BshFI GGCC 1 cut(s) 704
BshNI GGYRCC 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 532
BsiHKAI GWGCWC 2 cut(s) 487, 954
BsiSI CCGG 5 cut(s) 116, 144, 323, 666, 840
BsiWI CGTACG 1 cut(s) 903
BsmAI GTCTC 3 cut(s) 331, 434, 714
BsmBI CGTCTC 1 cut(s) 434
BsnI GGCC 1 cut(s) 704
Bso31I GGTCTC 1 cut(s) 331
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 487, 954
Bsp13I TCCGGA 1 cut(s) 665
Bsp143I GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
Bsp19I CCATGG 2 cut(s) 162, 478
BspACI CCGC 6 cut(s) 159, 249, 549, 596, 872, 962
BspANI GGCC 1 cut(s) 704
BspDI ATCGAT 1 cut(s) 565
BspEI TCCGGA 1 cut(s) 665
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 39
BspPI GGATC 4 cut(s) 350, 469, 559, 671
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 37
BspTNI GGTCTC 1 cut(s) 331
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 4 cut(s) 162, 182, 478, 752
BssMI GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 3 cut(s) 162, 478, 752
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 3 cut(s) 65, 645, 766
Bst6I CTCTTC 1 cut(s) 334
BstACI GRCGYC 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 266, 276
BstDEI CTNAG 1 cut(s) 919
BstDSI CCRYGG 2 cut(s) 162, 478
BstF5I GGATG 1 cut(s) 762
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 9 cut(s) 59, 358, 477, 532, 554, 612, 622, 679, 895
BstMAI GTCTC 3 cut(s) 331, 434, 714
BstMBI GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
BstMCI CGRYCG 1 cut(s) 532
BstMWI GCNNNNNNNGC 3 cut(s) 44, 443, 452
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 2 cut(s) 115, 181
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 2 cut(s) 116, 864
BstX2I RGATCY 4 cut(s) 474, 551, 676, 892
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 4 cut(s) 474, 551, 676, 892
Bsu15I ATCGAT 1 cut(s) 565
BsuRI GGCC 1 cut(s) 704
BsuTUI ATCGAT 1 cut(s) 565
BtgI CCRYGG 2 cut(s) 162, 478
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 762
BtsIMutI CAGTG 2 cut(s) 139, 775
Cac8I GCNNGC 2 cut(s) 266, 276
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 614
ClaI ATCGAT 1 cut(s) 565
CseI GACGC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 493, 904
CviAII CATG 7 cut(s) 163, 178, 213, 479, 658, 700, 742
CviQI GTAC 2 cut(s) 493, 904
DdeI CTNAG 1 cut(s) 919
DinI GGCGCC 1 cut(s) 39
DpnI GATC 9 cut(s) 58, 357, 476, 531, 553, 611, 621, 678, 894
DpnII GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 334
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 2 cut(s) 485, 952
Eco130I CCWWGG 3 cut(s) 162, 478, 752
Eco24I GRGCYC 2 cut(s) 487, 954
Eco31I GGTCTC 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 614
Eco53kI GAGCTC 2 cut(s) 485, 952
EcoICRI GAGCTC 2 cut(s) 485, 952
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 3 cut(s) 162, 478, 752
EcoT38I GRGCYC 2 cut(s) 487, 954
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 3 cut(s) 162, 478, 752
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 7 cut(s) 166, 181, 216, 482, 661, 703, 745
FatI CATG 7 cut(s) 162, 177, 212, 478, 657, 699, 741
Fnu4HI GCNGC 2 cut(s) 250, 435
FokI GGATG 1 cut(s) 769
FriOI GRGCYC 2 cut(s) 487, 954
Fsp4HI GCNGC 2 cut(s) 250, 435
FspBI CTAG 2 cut(s) 557, 753
GlaI GCGC 1 cut(s) 39
GluI GCNGC 2 cut(s) 250, 435
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 1 cut(s) 704
HapII CCGG 5 cut(s) 116, 144, 323, 666, 840
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 7 cut(s) 166, 181, 216, 482, 661, 703, 745
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 229, 526
HindII GTYRAC 2 cut(s) 229, 526
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 5 cut(s) 116, 144, 323, 666, 840
HphI GGTGA 1 cut(s) 633
Hpy166II GTNNAC 4 cut(s) 229, 238, 372, 526
Hpy188I TCNGA 5 cut(s) 154, 381, 614, 629, 635
Hpy188III TCNNGA 6 cut(s) 296, 406, 557, 666, 724, 929
Hpy8I GTNNAC 4 cut(s) 229, 238, 372, 526
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 3 cut(s) 65, 645, 766
HpyCH4IV ACGT 2 cut(s) 84, 827
HpyCH4V TGCA 2 cut(s) 437, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 443, 452
HpyF3I CTNAG 1 cut(s) 919
HpySE526I ACGT 2 cut(s) 84, 827
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 7 cut(s) 166, 181, 216, 482, 661, 703, 745
HspAI GCGC 1 cut(s) 38
KasI GGCGCC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 665
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
LmnI GCTCC 2 cut(s) 482, 964
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 1 cut(s) 261
MaeI CTAG 2 cut(s) 557, 753
MaeII ACGT 2 cut(s) 84, 827
MaeIII GTNAC 2 cut(s) 65, 542
MalI GATC 9 cut(s) 58, 357, 476, 531, 553, 611, 621, 678, 894
MboI GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
MboII GAAGA 7 cut(s) 116, 247, 250, 321, 869, 897, 921
MflI RGATCY 4 cut(s) 474, 551, 676, 892
MhlI GDGCHC 2 cut(s) 487, 954
MlsI TGGCCA 1 cut(s) 704
MluCI AATT 1 cut(s) 411
MluNI TGGCCA 1 cut(s) 704
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 2 cut(s) 512, 630
MmeI TCCRAC 1 cut(s) 607
MnlI CCTC 6 cut(s) 90, 113, 186, 387, 836, 874
Mox20I TGGCCA 1 cut(s) 704
MroI TCCGGA 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 704
MseI TTAA 3 cut(s) 389, 525, 819
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 964
MspI CCGG 5 cut(s) 116, 144, 323, 666, 840
MspR9I CCNGG 2 cut(s) 117, 183
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 3 cut(s) 44, 443, 452
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 1 cut(s) 117
NcoI CCATGG 2 cut(s) 162, 478
NdeII GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
NlaIII CATG 7 cut(s) 166, 181, 216, 482, 661, 703, 745
NlaIV GGNNCC 1 cut(s) 39
NspI RCATGY 1 cut(s) 181
NspV TTCGAA 1 cut(s) 602
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 534
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 8 cut(s) 27, 317, 562, 573, 630, 662, 720, 791
Pfl23II CGTACG 1 cut(s) 903
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 2 cut(s) 251, 436
Ple19I CGATCG 1 cut(s) 532
PleI GAGTC 2 cut(s) 512, 630
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 2 cut(s) 512, 630
Psp124BI GAGCTC 2 cut(s) 487, 954
Psp1406I AACGTT 1 cut(s) 827
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspLI CGTACG 1 cut(s) 903
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 614
PsuI RGATCY 4 cut(s) 474, 551, 676, 892
PvuI CGATCG 1 cut(s) 532
RsaI GTAC 2 cut(s) 494, 905
RsaNI GTAC 2 cut(s) 493, 904
SacI GAGCTC 2 cut(s) 487, 954
SaqAI TTAA 3 cut(s) 389, 525, 819
SatI GCNGC 2 cut(s) 250, 435
Sau3AI GATC 9 cut(s) 56, 355, 474, 529, 551, 609, 619, 676, 892
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 2 cut(s) 512, 630
ScrFI CCNGG 2 cut(s) 117, 183
SduI GDGCHC 2 cut(s) 487, 954
SfaNI GCATC 1 cut(s) 261
SfoI GGCGCC 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 1 cut(s) 614
Sse9I AATT 1 cut(s) 411
SsiI CCGC 6 cut(s) 159, 249, 549, 596, 872, 962
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 2 cut(s) 557, 753
SstI GAGCTC 2 cut(s) 487, 954
StyD4I CCNGG 2 cut(s) 115, 181
StyI CCWWGG 3 cut(s) 162, 478, 752
TaaI ACNGT 3 cut(s) 65, 645, 766
TaiI ACGT 2 cut(s) 87, 830
TaqI TCGA 8 cut(s) 207, 362, 532, 565, 591, 602, 723, 780
TasI AATT 1 cut(s) 411
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 8 cut(s) 27, 317, 562, 573, 630, 662, 720, 791
Tru1I TTAA 3 cut(s) 389, 525, 819
Tru9I TTAA 3 cut(s) 389, 525, 819
TscAI CASTG 2 cut(s) 139, 775
TseI GCWGC 1 cut(s) 434
TspDTI ATGAA 4 cut(s) 17, 527, 773, 820
TspGWI ACGGA 2 cut(s) 876, 921
TspRI CASTG 2 cut(s) 139, 775
VpaK11BI GGWCC 1 cut(s) 614
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 181
XmaJI CCTAGG 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 557, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.