RLG00000001500

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000785
Physical Location & Seq
Reverse (-)
16473571 .. 16473876
306 bp
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UTR
Exon/CDS
Intron
RLM00000001500

Sequence Viewer

Length: 306 bp
ATGAAGACACTTGGAGACAAAATTGAATGTCCCCACAGTCTTGTACCCATGAGAAAGGCTTTTAATGGTACCTTCATTGCTGTTGGTGGTTTTGACAGGGAAGATGGGAACAATGCTGTGGCTGAAGACAGTGCAGATCTTGTTGCTTTTGGTCGTTGGTTCTTGGCTAATCCAGATTTGCCAAAGAGATTTGAGCTTAATGCTCCTCTAAATAAGTACATCAGAGATACATTCTACATATCTGATCCGGTTCTTGGTTACACTGATTATCCATTTCTTGACAGCGCTGCTTGCGTTTCCAGCTGA

Protein Analysis

102

Amino Acids

11.2

Weight (kDa)

4.75

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 17 - 70 1.8e-09 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 68
AclWI GGATC 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 3 cut(s) 45, 70, 218
AfeI AGCGCT 1 cut(s) 286
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 1 cut(s) 26
AluBI AGCT 2 cut(s) 196, 303
AluI AGCT 2 cut(s) 196, 303
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 239
Aor51HI AGCGCT 1 cut(s) 286
ApeKI GCWGC 1 cut(s) 287
Asp718I GGTACC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 68
BbsI GAAGAC 2 cut(s) 11, 132
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 288
BglII AGATCT 1 cut(s) 136
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 70
BpiI GAAGAC 2 cut(s) 11, 132
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 75
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 68
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 15
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 15
Bsp143I GATC 2 cut(s) 136, 244
BspLI GGNNCC 1 cut(s) 70
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 75
BssMI GATC 2 cut(s) 136, 244
Bst4CI ACNGT 2 cut(s) 38, 131
BstC8I GCNNGC 1 cut(s) 292
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 2 cut(s) 139, 247
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 136, 244
BstMWI GCNNNNNNNGC 2 cut(s) 291, 300
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 2 cut(s) 11, 132
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 261
Cac8I GCNNGC 1 cut(s) 292
CfoI GCGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 44, 69, 217
CviAII CATG 1 cut(s) 49
CviJI RGCY 5 cut(s) 59, 122, 167, 196, 303
CviKI_1 RGCY 5 cut(s) 59, 122, 167, 196, 303
CviQI GTAC 3 cut(s) 44, 69, 217
DpnI GATC 2 cut(s) 138, 246
DpnII GATC 2 cut(s) 136, 244
Eco47III AGCGCT 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 144
FaeI CATG 1 cut(s) 52
FaiI YATR 2 cut(s) 50, 239
FaqI GGGAC 1 cut(s) 15
FatI CATG 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 1 cut(s) 286
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 288
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HpaII CCGG 1 cut(s) 248
Hpy188I TCNGA 2 cut(s) 224, 244
Hpy188III TCNNGA 2 cut(s) 173, 278
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 38, 131
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 300
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 285
KpnI GGTACC 1 cut(s) 72
Kzo9I GATC 2 cut(s) 136, 244
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 3 cut(s) 82, 186, 261
Lsp1109I GCAGC 1 cut(s) 274
MaeIII GTNAC 1 cut(s) 257
MalI GATC 2 cut(s) 138, 246
MboI GATC 2 cut(s) 136, 244
MboII GAAGA 3 cut(s) 16, 113, 137
MflI RGATCY 1 cut(s) 136
MluCI AATT 1 cut(s) 21
MnlI CCTC 1 cut(s) 216
MseI TTAA 2 cut(s) 63, 198
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 291, 300
NdeII GATC 2 cut(s) 136, 244
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 70
PkrI GCNGC 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 70
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 3 cut(s) 45, 70, 218
RsaNI GTAC 3 cut(s) 44, 69, 217
SaqAI TTAA 2 cut(s) 63, 198
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 2 cut(s) 136, 244
SetI ASST 3 cut(s) 74, 198, 305
Sse9I AATT 1 cut(s) 21
TaaI ACNGT 2 cut(s) 38, 131
TasI AATT 1 cut(s) 21
TatI WGTACW 1 cut(s) 216
Tru1I TTAA 2 cut(s) 63, 198
Tru9I TTAA 2 cut(s) 63, 198
TscAI CASTG 2 cut(s) 136, 268
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 17, 64
TspRI CASTG 2 cut(s) 136, 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.