AT1G76690

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000785
Physical Location & Seq
Forward (+)
28778622 .. 28780483
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76690.1

Sequence Viewer

Length: 1125 bp
ATGGAAATGGTAAACGCAGAAGCAAAACAGAGTGTCCCTCTTCTCACCCCTTATAAGATGGGAAGATTCAATCTTTCTCATAGGGTTGTTCTAGCACCATTGACGAGACAGAAATCGTACGGAAGCGTTCCTCAGCCTCACGCTATCTTGTATTACTCTCAGAGAACGTCACCGGGAGGTTTTCTCATCGCTGAAGCCACCGGGGTTTCAGATACAGCTCAAGGGTATCCAGATACACCTGGGATATGGACTAAAGAGCATGTGGAGGCATGGAAGCCAATCGTTGATGCTGTACATGCCAAAGGTGGTATCTTCTTCTGTCAGATCTGGCATGTTGGCCGCGTTTCTAATCGCGGTTTTCAGCCAAGGAGGCAAGCTCCTATCTCTTGTACGGGGAAGCCAATTATGCCTCAAATGCGTGCTAATGGCATTGATGAAGCTCGCTTTACCCCTCCAAGACGTCTAAGTATCGAAGAAATCCCCGGCATTGTCAATGATTTTAGGCTTGCTGCAAGAAATGCTATGGAAGCTGGTTTTGATGGAGTTGAGATTCATGGAGCTCATGGCTATCTGATTGATCAGTTCATGAAGGACAAAGTGAATGACAGAACTGATGAATATGGTGGATCATTGCAAAACCGTTGCAAATTTGCTCTGGAAGTAGTCGATGCAGTGGCTAAGGAGATCGGGCCAGACCGTGTTGGAATCAGGCTCTCTCCGTTTGCAGACTATATGGAATCCGGAGACACTAATCCAGAAGCATTAGGGCTGTACATGGTGGAATCTCTGAACAAATATGGAATCCTCTACTGTCATATGATTGAACCCAGAATGAAAACAGTGGGAGAAATAGCAGCGTGTTCTCACACACTAATGCCAATGAGGGAAGCCTTTAAGGGGACTTTCATCTCTGCAGGAGGTTTCACGAGGGAAGATGGGAATGAGGCCGTGGCAAAGGGACGAACTGATCTTGTGGCTTATGGTCGATGGTTTCTAGCCAACCCAGACCTGCCAAAGCGGTTCCAACTGGATGCACCGCTGAATAAATACAATAGGTCAACGTTTTACACTTCTGATCCTGTCGTGGGTTACACCGATTACCCTTCCCTTGAATCAACAGCTTAA

Protein Analysis

374

Amino Acids

41.57

Weight (kDa)

7.12

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 14 - 346 6.2e-85 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
AatII GACGTC 1 cut(s) 463
Acc36I ACCTGC 1 cut(s) 1017
AccII CGCG 2 cut(s) 342, 354
AccIII TCCGGA 1 cut(s) 740
AciI CCGC 4 cut(s) 340, 354, 1018, 1037
AclI AACGTT 1 cut(s) 1061
AclWI GGATC 2 cut(s) 634, 1070
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 460
AfaI GTAC 4 cut(s) 119, 294, 391, 773
AfiI CCNNNNNNNGG 2 cut(s) 897, 1085
AgsI TTSAA 3 cut(s) 70, 824, 1112
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 6 cut(s) 218, 377, 440, 530, 560, 1121
AluI AGCT 6 cut(s) 218, 377, 440, 530, 560, 1121
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 2 cut(s) 100, 738
AlwI GGATC 2 cut(s) 634, 1070
Aor13HI TCCGGA 1 cut(s) 740
AoxI GGCC 3 cut(s) 337, 689, 945
ApeKI GCWGC 2 cut(s) 509, 854
ApoI RAATTY 1 cut(s) 647
AspS9I GGNCC 1 cut(s) 689
AsuC2I CCSGG 3 cut(s) 174, 202, 483
AsuHPI GGTGA 2 cut(s) 37, 162
BanII GRGCYC 1 cut(s) 562
BauI CACGAG 1 cut(s) 925
Bbv12I GWGCWC 1 cut(s) 562
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 496, 866
BccI CCATC 4 cut(s) 52, 533, 929, 981
BceAI ACGGC 1 cut(s) 932
BciT130I CCWGG 1 cut(s) 240
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 3 cut(s) 174, 202, 483
BcoDI GTCTC 2 cut(s) 100, 738
BfaI CTAG 2 cut(s) 92, 995
BfmI CTRYAG 1 cut(s) 912
BfuAI ACCTGC 1 cut(s) 1017
BfuI GTATCC 1 cut(s) 237
BglI GCCNNNNNGGC 1 cut(s) 370
BglII AGATCT 1 cut(s) 324
BisI GCNGC 3 cut(s) 340, 510, 855
BlsI GCNGC 3 cut(s) 341, 511, 856
Bme1390I CCNGG 4 cut(s) 174, 202, 240, 483
BmgT120I GGNCC 1 cut(s) 689
BmiI GGNNCC 1 cut(s) 1022
BmrFI CCNGG 4 cut(s) 174, 202, 240, 483
BmsI GCATC 3 cut(s) 277, 658, 1021
BplI GAGNNNNNCTC 6 cut(s) 22, 54, 168, 200, 361, 393
Bpu10I CCTNAGC 2 cut(s) 132, 678
BpuEI CTTGAG 1 cut(s) 204
BpuMI CCSGG 3 cut(s) 174, 202, 483
BsaHI GRCGYC 1 cut(s) 460
BsaJI CCNNGG 5 cut(s) 201, 239, 365, 481, 948
BsaWI WCCGGW 1 cut(s) 740
Bsc4I CCNNNNNNNGG 2 cut(s) 897, 1085
Bse1I ACTGG 1 cut(s) 1032
Bse3DI GCAATG 1 cut(s) 629
BseAI TCCGGA 1 cut(s) 740
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 5 cut(s) 201, 239, 365, 481, 948
BseGI GGATG 1 cut(s) 1036
BseLI CCNNNNNNNGG 2 cut(s) 897, 1085
BseMI GCAATG 1 cut(s) 629
BseMII CTCAG 2 cut(s) 146, 173
BseNI ACTGG 1 cut(s) 1032
BseXI GCAGC 2 cut(s) 496, 866
Bsh1236I CGCG 2 cut(s) 342, 354
BshFI GGCC 3 cut(s) 339, 691, 947
BsiHKAI GWGCWC 1 cut(s) 562
BsiSI CCGG 4 cut(s) 173, 201, 483, 741
BsiWI CGTACG 1 cut(s) 117
BslFI GGGAC 3 cut(s) 20, 913, 972
BslI CCNNNNNNNGG 2 cut(s) 897, 1085
BsmAI GTCTC 2 cut(s) 100, 738
BsmFI GGGAC 3 cut(s) 20, 913, 972
BsnI GGCC 3 cut(s) 339, 691, 947
Bsp1286I GDGCHC 1 cut(s) 562
Bsp13I TCCGGA 1 cut(s) 740
Bsp1407I TGTACA 2 cut(s) 292, 771
Bsp143I GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
BspACI CCGC 4 cut(s) 340, 354, 1018, 1037
BspANI GGCC 3 cut(s) 339, 691, 947
BspCNI CTCAG 2 cut(s) 145, 172
BspEI TCCGGA 1 cut(s) 740
BspFNI CGCG 2 cut(s) 342, 354
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 1022
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 1 cut(s) 1017
BspPI GGATC 2 cut(s) 634, 1070
BsrDI GCAATG 1 cut(s) 629
BsrGI TGTACA 2 cut(s) 292, 771
BsrI ACTGG 1 cut(s) 1032
BssECI CCNNGG 5 cut(s) 201, 239, 365, 481, 948
BssMI GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
BssNI GRCGYC 1 cut(s) 460
BssSI CACGAG 1 cut(s) 925
BssT1I CCWWGG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 925
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 4 cut(s) 641, 698, 812, 841
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 460
BstAPI GCANNNNNTGC 1 cut(s) 518
BstAUI TGTACA 2 cut(s) 292, 771
BstC8I GCNNGC 4 cut(s) 375, 420, 442, 507
BstDEI CTNAG 4 cut(s) 132, 159, 464, 678
BstDSI CCRYGG 1 cut(s) 948
BstF5I GGATG 1 cut(s) 1036
BstFNI CGCG 2 cut(s) 342, 354
BstKTI GATC 6 cut(s) 327, 580, 629, 687, 970, 1078
BstMAI GTCTC 2 cut(s) 100, 738
BstMBI GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
BstMWI GCNNNNNNNGC 6 cut(s) 296, 370, 406, 415, 518, 527
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 3 cut(s) 263, 299, 335
BstSCI CCNGG 4 cut(s) 172, 200, 238, 481
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 2 cut(s) 342, 354
BstV1I GCAGC 2 cut(s) 496, 866
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 3 cut(s) 339, 691, 947
BtgI CCRYGG 1 cut(s) 948
BtgZI GCGATG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 1036
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 2 cut(s) 678, 846
BveI ACCTGC 1 cut(s) 1017
Cac8I GCNNGC 4 cut(s) 375, 420, 442, 507
CciI TCATGA 1 cut(s) 585
Cfr13I GGNCC 1 cut(s) 689
Csp6I GTAC 4 cut(s) 118, 293, 390, 772
CviAII CATG 8 cut(s) 260, 270, 296, 332, 554, 563, 586, 775
CviQI GTAC 4 cut(s) 118, 293, 390, 772
DdeI CTNAG 4 cut(s) 132, 159, 464, 678
DpnI GATC 6 cut(s) 326, 579, 628, 686, 969, 1077
DpnII GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 562
Eco53kI GAGCTC 1 cut(s) 560
Eco57I CTGAAG 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 8 cut(s) 263, 273, 299, 335, 557, 566, 589, 778
FaqI GGGAC 3 cut(s) 20, 913, 972
FatI CATG 8 cut(s) 259, 269, 295, 331, 553, 562, 585, 774
FauNDI CATATG 1 cut(s) 816
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 3 cut(s) 340, 510, 855
FokI GGATG 1 cut(s) 1043
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 3 cut(s) 340, 510, 855
FspBI CTAG 2 cut(s) 92, 995
GluI GCNGC 3 cut(s) 340, 510, 855
HaeIII GGCC 3 cut(s) 339, 691, 947
HapII CCGG 4 cut(s) 173, 201, 483, 741
Hin1I GRCGYC 1 cut(s) 460
Hin1II CATG 8 cut(s) 263, 273, 299, 335, 557, 566, 589, 778
HincII GTYRAC 1 cut(s) 1059
HindII GTYRAC 1 cut(s) 1059
HinfI GANTC 7 cut(s) 66, 550, 705, 737, 782, 801, 1112
HpaII CCGG 4 cut(s) 173, 201, 483, 741
HphI GGTGA 2 cut(s) 37, 162
Hpy166II GTNNAC 2 cut(s) 13, 1059
Hpy188I TCNGA 6 cut(s) 162, 211, 324, 573, 789, 1075
Hpy188III TCNNGA 6 cut(s) 230, 586, 656, 741, 755, 925
Hpy8I GTNNAC 2 cut(s) 13, 1059
HpyAV CCTTC 2 cut(s) 583, 1113
HpyCH4III ACNGT 4 cut(s) 641, 698, 812, 841
HpyCH4IV ACGT 3 cut(s) 167, 460, 1061
HpyCH4V TGCA 7 cut(s) 512, 634, 645, 671, 725, 914, 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 296, 370, 406, 415, 518, 527
HpyF3I CTNAG 4 cut(s) 132, 159, 464, 678
HpySE526I ACGT 3 cut(s) 167, 460, 1061
Hsp92I GRCGYC 1 cut(s) 460
Hsp92II CATG 8 cut(s) 263, 273, 299, 335, 557, 566, 589, 778
Kpn2I TCCGGA 1 cut(s) 740
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
LmnI GCTCC 2 cut(s) 382, 557
Lsp1109I GCAGC 2 cut(s) 496, 866
LweI GCATC 3 cut(s) 277, 658, 1021
MaeI CTAG 2 cut(s) 92, 995
MaeII ACGT 3 cut(s) 167, 460, 1061
MaeIII GTNAC 2 cut(s) 168, 1088
MalI GATC 6 cut(s) 326, 579, 628, 686, 969, 1077
MboI GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
MboII GAAGA 6 cut(s) 32, 75, 304, 307, 485, 944
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 2 cut(s) 402, 647
MmeI TCCRAC 2 cut(s) 682, 1048
MroI TCCGGA 1 cut(s) 740
MseI TTAA 2 cut(s) 894, 1123
MslI CAYNNNNRTG 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 1039
MspI CCGG 4 cut(s) 173, 201, 483, 741
MspR9I CCNGG 4 cut(s) 174, 202, 240, 483
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 2 cut(s) 342, 354
MwoI GCNNNNNNNGC 6 cut(s) 296, 370, 406, 415, 518, 527
NciI CCSGG 3 cut(s) 174, 202, 483
NdeI CATATG 1 cut(s) 816
NdeII GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
NlaIII CATG 8 cut(s) 263, 273, 299, 335, 557, 566, 589, 778
NlaIV GGNNCC 1 cut(s) 1022
NmuCI GTSAC 1 cut(s) 168
NspI RCATGY 3 cut(s) 263, 299, 335
PagI TCATGA 1 cut(s) 585
PfeI GAWTC 7 cut(s) 66, 550, 705, 737, 782, 801, 1112
Pfl23II CGTACG 1 cut(s) 117
PkrI GCNGC 3 cut(s) 341, 511, 856
PsiI TTATAA 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 562
Psp1406I AACGTT 1 cut(s) 1061
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspLI CGTACG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 1022
PspPI GGNCC 1 cut(s) 689
PstI CTGCAG 1 cut(s) 916
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 4 cut(s) 119, 294, 391, 773
RsaNI GTAC 4 cut(s) 118, 293, 390, 772
RseI CAYNNNNRTG 1 cut(s) 872
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 2 cut(s) 894, 1123
SatI GCNGC 3 cut(s) 340, 510, 855
Sau3AI GATC 6 cut(s) 324, 577, 626, 684, 967, 1075
Sau96I GGNCC 1 cut(s) 689
ScrFI CCNGG 4 cut(s) 174, 202, 240, 483
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 3 cut(s) 277, 658, 1021
SfcI CTRYAG 1 cut(s) 912
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 2 cut(s) 402, 647
SsiI CCGC 4 cut(s) 340, 354, 1018, 1037
SspMI CTAG 2 cut(s) 92, 995
SstI GAGCTC 1 cut(s) 562
StyD4I CCNGG 4 cut(s) 172, 200, 238, 481
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 4 cut(s) 641, 698, 812, 841
TaiI ACGT 3 cut(s) 170, 463, 1064
TaqI TCGA 3 cut(s) 471, 666, 985
TasI AATT 2 cut(s) 402, 647
TatI WGTACW 2 cut(s) 292, 771
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 7 cut(s) 66, 550, 705, 737, 782, 801, 1112
Tru1I TTAA 2 cut(s) 894, 1123
Tru9I TTAA 2 cut(s) 894, 1123
TscAI CASTG 2 cut(s) 678, 846
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 2 cut(s) 509, 854
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 7 cut(s) 450, 542, 574, 602, 630, 848, 895
TspGWI ACGGA 2 cut(s) 135, 708
TspRI CASTG 2 cut(s) 678, 846
XapI RAATTY 1 cut(s) 647
XceI RCATGY 3 cut(s) 263, 299, 335
XspI CTAG 2 cut(s) 92, 995
ZraI GACGTC 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.