AT1G76680

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000785
Physical Location & Seq
Forward (+)
28776626 .. 28778837
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76680.1

Sequence Viewer

Length: 1119 bp
ATGGAAAACGGAGAAGCAAAACAGAGTGTACCTCTTCTCACTCCCTATAAGATGGGAAGATTCAATCTTTCCCATAGGGTTGTTCTAGCACCATTGACGAGACAGAGATCGTACGGAAACGTTCCTCAGCCTCACGCTGCCATATATTACTCTCAGAGAACGACTCCAGGAGGTTTTCTCATCACTGAAGCCACTGGAGTTTCAGATACAGCTCAAGGATATCAAGATACTCCTGGGATATGGACTAAAGAGCATGTGGAGGCATGGAAGCCAATCGTTGATGCTGTACATGCCAAAGGTGGTATCTTCTTCTGTCAGATCTGGCATGTTGGCCGCGTTTCTAATAGCGGGTTTCAGCCAAATGGAAAAGCTCCTATCTCTTGTTCGGATAAGCCATTGATGCCTCAAATTCGCTCTAATGGCATCGATGAAGCTCTCTTTACCCCTCCAAGACGGCTTGGTATCGAAGAAATCCCCGGCATTGTCAATGATTTTAGGCTTGCTGCAAGAAATGCTATGGAAGCTGGTTTTGATGGAGTTGAGATTCATGGAGCTAATGGCTATCTGATTGACCAGTTCATGAAGGATACGGTGAATGATAGAACTGATGAATACGGTGGATCATTGCAAAACCGTTGCAAATTTCCTCTAGAAATAGTCGATGCAGTTGCTAAGGAGATCGGACCAGACCGTGTTGGAATCAGGCTCTCTCCATTTGCTGACTACATGGAATCTGGAGACACTAATCCAGGAGCATTAGGGCTTTATATGGCGGAATCTTTGAACAAATACGGAATCCTCTACTGTCATGTGATTGAAGCGAGAATGAAAACAATGGGAGAAGTACATGCTTGTCCTCACACACTAATGCCGATGAGGAAAGCGTTTAAGGGGACTTTTATCTCCGCAGGAGGTTTCACGAGGGAAGATGGGAATGAGGCTGTGTCAAAGGGAAGAACTGATTTGGTGGCTTATGGTCGATGGTTTCTAGCCAACCCGGACCTGCCAAAGAGGTTCCAAGTGGATGCACCGCTGAATAAGTACGATAGACCAACGTTTTACACTTCTGATCCAGTCGTCGGTTACACCGATTACCCTTTCCTCGAATCAACAGCTTAA

Protein Analysis

372

Amino Acids

41.17

Weight (kDa)

6.32

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 12 - 345 2.4e-84 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1011
AccII CGCG 1 cut(s) 336
AciI CCGC 5 cut(s) 334, 348, 773, 906, 1031
AclI AACGTT 2 cut(s) 120, 1055
AclWI GGATC 2 cut(s) 628, 1064
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 2 cut(s) 408, 641
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 5 cut(s) 30, 113, 288, 846, 1043
AfiI CCNNNNNNNGG 1 cut(s) 1079
AgsI TTSAA 3 cut(s) 64, 784, 818
AjnI CCWGG 3 cut(s) 166, 232, 748
AluBI AGCT 6 cut(s) 212, 371, 434, 524, 554, 1115
AluI AGCT 6 cut(s) 212, 371, 434, 524, 554, 1115
Alw26I GTCTC 2 cut(s) 94, 732
AlwI GGATC 2 cut(s) 628, 1064
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 2 cut(s) 137, 503
ApoI RAATTY 2 cut(s) 408, 641
AspS9I GGNCC 2 cut(s) 683, 1000
AsuC2I CCSGG 2 cut(s) 477, 998
AsuHPI GGTGA 1 cut(s) 604
AvaII GGWCC 2 cut(s) 683, 1000
BauI CACGAG 1 cut(s) 919
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 124, 490
BccI CCATC 4 cut(s) 46, 527, 923, 975
BceAI ACGGC 1 cut(s) 470
BcgI CGANNNNNNTGC 2 cut(s) 650, 684
BciT130I CCWGG 3 cut(s) 168, 234, 750
BciVI GTATCC 1 cut(s) 580
BcnI CCSGG 2 cut(s) 477, 998
BcoDI GTCTC 2 cut(s) 94, 732
BfaI CTAG 3 cut(s) 86, 650, 989
BfuAI ACCTGC 1 cut(s) 1011
BfuI GTATCC 1 cut(s) 580
BglII AGATCT 1 cut(s) 318
BisI GCNGC 3 cut(s) 138, 334, 504
BlsI GCNGC 3 cut(s) 139, 335, 505
Bme1390I CCNGG 5 cut(s) 168, 234, 477, 750, 998
Bme18I GGWCC 2 cut(s) 683, 1000
BmgT120I GGNCC 2 cut(s) 683, 1000
BmiI GGNNCC 1 cut(s) 1016
BmrFI CCNGG 5 cut(s) 168, 234, 477, 750, 998
BmsI GCATC 5 cut(s) 271, 390, 432, 652, 1015
BplI GAGNNNNNCTC 6 cut(s) 16, 48, 148, 180, 162, 194
BpmI CTGGAG 3 cut(s) 150, 216, 756
Bpu10I CCTNAGC 2 cut(s) 126, 672
BpuEI CTTGAG 1 cut(s) 198
BpuMI CCSGG 2 cut(s) 477, 998
Bsa29I ATCGAT 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 233, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 1079
Bse1I ACTGG 3 cut(s) 199, 574, 1073
Bse3DI GCAATG 1 cut(s) 623
BseBI CCWGG 3 cut(s) 168, 234, 750
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 233, 475
BseGI GGATG 1 cut(s) 1030
BseLI CCNNNNNNNGG 1 cut(s) 1079
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 2 cut(s) 140, 167
BseNI ACTGG 3 cut(s) 199, 574, 1073
BseXI GCAGC 2 cut(s) 124, 490
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 333
BshVI ATCGAT 1 cut(s) 426
BsiSI CCGG 2 cut(s) 477, 998
BsiWI CGTACG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 907
BslI CCNNNNNNNGG 1 cut(s) 1079
BsmAI GTCTC 2 cut(s) 94, 732
BsmFI GGGAC 1 cut(s) 907
BsnI GGCC 1 cut(s) 333
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 5 cut(s) 107, 318, 620, 678, 1069
BspACI CCGC 5 cut(s) 334, 348, 773, 906, 1031
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 2 cut(s) 139, 166
BspDI ATCGAT 1 cut(s) 426
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 1 cut(s) 1016
BspMI ACCTGC 1 cut(s) 1011
BspPI GGATC 2 cut(s) 628, 1064
BsrDI GCAATG 1 cut(s) 623
BsrGI TGTACA 1 cut(s) 286
BsrI ACTGG 3 cut(s) 199, 574, 1073
BssECI CCNNGG 2 cut(s) 233, 475
BssMI GATC 5 cut(s) 107, 318, 620, 678, 1069
BssSI CACGAG 1 cut(s) 919
Bst2BI CACGAG 1 cut(s) 919
Bst2UI CCWGG 3 cut(s) 168, 234, 750
Bst4CI ACNGT 5 cut(s) 592, 617, 635, 692, 806
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 3 cut(s) 126, 153, 672
BstF5I GGATG 1 cut(s) 1030
BstFNI CGCG 1 cut(s) 336
BstKTI GATC 5 cut(s) 110, 321, 623, 681, 1072
BstMAI GTCTC 2 cut(s) 94, 732
BstMBI GATC 5 cut(s) 107, 318, 620, 678, 1069
BstMWI GCNNNNNNNGC 5 cut(s) 290, 400, 420, 512, 521
BstNI CCWGG 3 cut(s) 168, 234, 750
BstNSI RCATGY 4 cut(s) 257, 293, 329, 851
BstSCI CCNGG 5 cut(s) 166, 232, 475, 748, 996
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 2 cut(s) 124, 490
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
Bsu15I ATCGAT 1 cut(s) 426
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 1 cut(s) 333
BsuTUI ATCGAT 1 cut(s) 426
BtsCI GGATG 1 cut(s) 1030
BtsIMutI CAGTG 2 cut(s) 183, 192
BveI ACCTGC 1 cut(s) 1011
Cac8I GCNNGC 1 cut(s) 501
CciI TCATGA 1 cut(s) 579
Cfr13I GGNCC 2 cut(s) 683, 1000
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 5 cut(s) 29, 112, 287, 845, 1042
CviAII CATG 9 cut(s) 254, 264, 290, 326, 548, 580, 727, 809, 848
CviQI GTAC 5 cut(s) 29, 112, 287, 845, 1042
DdeI CTNAG 3 cut(s) 126, 153, 672
DpnI GATC 5 cut(s) 109, 320, 622, 680, 1071
DpnII GATC 5 cut(s) 107, 318, 620, 678, 1069
EaeI YGGCCR 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 788
Eco32I GATATC 1 cut(s) 221
Eco47I GGWCC 2 cut(s) 683, 1000
Eco57I CTGAAG 1 cut(s) 207
EcoRII CCWGG 3 cut(s) 166, 232, 748
EcoRV GATATC 1 cut(s) 221
FaeI CATG 9 cut(s) 257, 267, 293, 329, 551, 583, 730, 812, 851
FaqI GGGAC 1 cut(s) 907
FatI CATG 9 cut(s) 253, 263, 289, 325, 547, 579, 726, 808, 847
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 3 cut(s) 138, 334, 504
FokI GGATG 1 cut(s) 1037
Fsp4HI GCNGC 3 cut(s) 138, 334, 504
FspBI CTAG 3 cut(s) 86, 650, 989
GluI GCNGC 3 cut(s) 138, 334, 504
GsuI CTGGAG 3 cut(s) 150, 216, 756
HaeIII GGCC 1 cut(s) 333
HapII CCGG 2 cut(s) 477, 998
Hin1II CATG 9 cut(s) 257, 267, 293, 329, 551, 583, 730, 812, 851
HinfI GANTC 8 cut(s) 60, 163, 544, 699, 731, 776, 795, 1106
HpaII CCGG 2 cut(s) 477, 998
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 7 cut(s) 156, 205, 318, 388, 567, 683, 1069
Hpy188III TCNNGA 5 cut(s) 224, 580, 650, 735, 919
Hpy8I GTNNAC 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 1082
HpyAV CCTTC 1 cut(s) 577
HpyCH4III ACNGT 5 cut(s) 592, 617, 635, 692, 806
HpyCH4IV ACGT 2 cut(s) 120, 1055
HpyCH4V TGCA 5 cut(s) 506, 628, 639, 665, 1028
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 400, 420, 512, 521
HpyF3I CTNAG 3 cut(s) 126, 153, 672
HpySE526I ACGT 2 cut(s) 120, 1055
Hsp92II CATG 9 cut(s) 257, 267, 293, 329, 551, 583, 730, 812, 851
Kzo9I GATC 5 cut(s) 107, 318, 620, 678, 1069
LmnI GCTCC 3 cut(s) 376, 551, 752
Lsp1109I GCAGC 2 cut(s) 124, 490
LweI GCATC 5 cut(s) 271, 390, 432, 652, 1015
MaeI CTAG 3 cut(s) 86, 650, 989
MaeII ACGT 2 cut(s) 120, 1055
MaeIII GTNAC 1 cut(s) 1082
MalI GATC 5 cut(s) 109, 320, 622, 680, 1071
MboI GATC 5 cut(s) 107, 318, 620, 678, 1069
MboII GAAGA 7 cut(s) 26, 69, 298, 301, 479, 938, 966
MflI RGATCY 1 cut(s) 318
MluCI AATT 2 cut(s) 408, 641
MlyI GAGTC 1 cut(s) 157
MmeI TCCRAC 1 cut(s) 676
MseI TTAA 2 cut(s) 888, 1117
MslI CAYNNNNRTG 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 1033
MspI CCGG 2 cut(s) 477, 998
MspR9I CCNGG 5 cut(s) 168, 234, 477, 750, 998
MvaI CCWGG 3 cut(s) 168, 234, 750
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 5 cut(s) 290, 400, 420, 512, 521
NciI CCSGG 2 cut(s) 477, 998
NdeII GATC 5 cut(s) 107, 318, 620, 678, 1069
NlaIII CATG 9 cut(s) 257, 267, 293, 329, 551, 583, 730, 812, 851
NlaIV GGNNCC 1 cut(s) 1016
NspI RCATGY 4 cut(s) 257, 293, 329, 851
PagI TCATGA 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 1086
PfeI GAWTC 7 cut(s) 60, 544, 699, 731, 776, 795, 1106
Pfl23II CGTACG 1 cut(s) 111
PfoI TCCNGGA 2 cut(s) 166, 748
PkrI GCNGC 3 cut(s) 139, 335, 505
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Psp1406I AACGTT 2 cut(s) 120, 1055
Psp6I CCWGG 3 cut(s) 166, 232, 748
PspGI CCWGG 3 cut(s) 166, 232, 748
PspLI CGTACG 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 1016
PspPI GGNCC 2 cut(s) 683, 1000
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 5 cut(s) 30, 113, 288, 846, 1043
RsaNI GTAC 5 cut(s) 29, 112, 287, 845, 1042
RseI CAYNNNNRTG 1 cut(s) 866
SaqAI TTAA 2 cut(s) 888, 1117
SatI GCNGC 3 cut(s) 138, 334, 504
Sau3AI GATC 5 cut(s) 107, 318, 620, 678, 1069
Sau96I GGNCC 2 cut(s) 683, 1000
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 5 cut(s) 168, 234, 477, 750, 998
SfaNI GCATC 5 cut(s) 271, 390, 432, 652, 1015
SinI GGWCC 2 cut(s) 683, 1000
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 2 cut(s) 408, 641
SsiI CCGC 5 cut(s) 334, 348, 773, 906, 1031
SspMI CTAG 3 cut(s) 86, 650, 989
StyD4I CCNGG 5 cut(s) 166, 232, 475, 748, 996
TaaI ACNGT 5 cut(s) 592, 617, 635, 692, 806
TaiI ACGT 2 cut(s) 123, 1058
TaqI TCGA 5 cut(s) 426, 465, 660, 979, 1104
TasI AATT 2 cut(s) 408, 641
TatI WGTACW 2 cut(s) 286, 844
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 7 cut(s) 60, 544, 699, 731, 776, 795, 1106
Tru1I TTAA 2 cut(s) 888, 1117
Tru9I TTAA 2 cut(s) 888, 1117
TscAI CASTG 2 cut(s) 190, 199
TseI GCWGC 2 cut(s) 137, 503
TspDTI ATGAA 6 cut(s) 444, 536, 568, 596, 624, 842
TspGWI ACGGA 3 cut(s) 24, 129, 807
TspRI CASTG 2 cut(s) 190, 199
VpaK11BI GGWCC 2 cut(s) 683, 1000
XapI RAATTY 2 cut(s) 408, 641
XbaI TCTAGA 1 cut(s) 649
XceI RCATGY 4 cut(s) 257, 293, 329, 851
XspI CTAG 3 cut(s) 86, 650, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.