Rmu_sc0033307.1_g000001

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0033307.1
OG0000785
Physical Location & Seq
Forward (+)
1 .. 1853
1853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0033307.1_g000001.1.cds

Sequence Viewer

Length: 460 bp
agttgttttagcgccattgactagacagcgatcatatggaaatgttccccagccacatgccatcttatattactctcagagaacatctaaaggaggtcttctcataactgaagccactggagtttctgacacagcacaagggtatccagatactcctggtatatggacaaaggagcaagttgaagcatggaaacccattgtcaatgctgtacatgctaaagggggtatcttcttctgtcagatttggcatgtggggagggtttcaaatactggctttcagccaaatgggcaggttcccatctcttctactgagaagcccctaaccccccaaatacgatctaatggcattgatgttgcgcaaacacctccaagacgattaaggacagatgaaattcctcaaattgtcaatgattttagacttgctgcaaggaatgctatggaagctggtaatcttttttaa

Protein Analysis

152

Amino Acids

16.71

Weight (kDa)

9.51

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 281
AcsI RAATTY 1 cut(s) 391
AcuI CTGAAG 1 cut(s) 130
AfaI GTAC 1 cut(s) 211
AgsI TTSAA 2 cut(s) 183, 265
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 1 cut(s) 444
AluI AGCT 1 cut(s) 444
ApeKI GCWGC 1 cut(s) 423
ApoI RAATTY 1 cut(s) 391
AspLEI GCGC 2 cut(s) 14, 359
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 410
BccI CCATC 2 cut(s) 69, 306
BciT130I CCWGG 1 cut(s) 157
BciVI GTATCC 1 cut(s) 154
BfaI CTAG 1 cut(s) 22
BfoI RGCGCY 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 281
BfuI GTATCC 1 cut(s) 154
BglI GCCNNNNNGGC 1 cut(s) 287
BisI GCNGC 1 cut(s) 424
BlsI GCNGC 1 cut(s) 425
Bme1390I CCNGG 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 85, 117
BpmI CTGGAG 1 cut(s) 139
Bse1I ACTGG 2 cut(s) 122, 275
BseBI CCWGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 90, 301
BseNI ACTGG 2 cut(s) 122, 275
BseXI GCAGC 1 cut(s) 410
BseYI CCCAGC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 437
Bsp1407I TGTACA 1 cut(s) 209
Bsp143I GATC 2 cut(s) 30, 336
BspCNI CTCAG 2 cut(s) 89, 302
BspLI GGNNCC 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 209
BsrI ACTGG 2 cut(s) 122, 275
BssMI GATC 2 cut(s) 30, 336
Bst2UI CCWGG 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 308
BstAPI GCANNNNNTGC 1 cut(s) 432
BstAUI TGTACA 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 76, 310
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 359
BstKTI GATC 2 cut(s) 33, 339
BstMBI GATC 2 cut(s) 30, 336
BstMWI GCNNNNNNNGC 4 cut(s) 213, 287, 432, 441
BstNI CCWGG 1 cut(s) 157
BstNSI RCATGY 3 cut(s) 60, 216, 252
BstSCI CCNGG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 410
BstV2I GAAGAC 1 cut(s) 90
BsuI GTATCC 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 115
BveI ACCTGC 1 cut(s) 281
CfoI GCGC 2 cut(s) 14, 359
Csp6I GTAC 1 cut(s) 210
CviAII CATG 4 cut(s) 57, 187, 213, 249
CviJI RGCY 6 cut(s) 53, 114, 274, 281, 317, 444
CviKI_1 RGCY 6 cut(s) 53, 114, 274, 281, 317, 444
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 2 cut(s) 76, 310
DpnI GATC 2 cut(s) 32, 338
DpnII GATC 2 cut(s) 30, 336
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 155
FaeI CATG 4 cut(s) 60, 190, 216, 252
FalI AAGNNNNNCTT 2 cut(s) 82, 114
FatI CATG 4 cut(s) 56, 186, 212, 248
FauNDI CATATG 1 cut(s) 35
Fnu4HI GCNGC 1 cut(s) 424
Fsp4HI GCNGC 1 cut(s) 424
FspBI CTAG 1 cut(s) 22
FspI TGCGCA 1 cut(s) 358
GlaI GCGC 2 cut(s) 13, 358
GluI GCNGC 1 cut(s) 424
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 1 cut(s) 139
HaeII RGCGCY 1 cut(s) 15
HhaI GCGC 2 cut(s) 14, 359
Hin1II CATG 4 cut(s) 60, 190, 216, 252
Hin6I GCGC 2 cut(s) 12, 357
HinP1I GCGC 2 cut(s) 12, 357
Hpy188I TCNGA 3 cut(s) 79, 128, 241
Hpy188III TCNNGA 1 cut(s) 147
HpyCH4V TGCA 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 287, 432, 441
HpyF3I CTNAG 2 cut(s) 76, 310
Hsp92II CATG 4 cut(s) 60, 190, 216, 252
HspAI GCGC 2 cut(s) 12, 357
Kzo9I GATC 2 cut(s) 30, 336
LmnI GCTCC 1 cut(s) 173
LpnPI CCDG 8 cut(s) 63, 103, 142, 160, 169, 256, 276, 430
Lsp1109I GCAGC 1 cut(s) 410
MaeI CTAG 1 cut(s) 22
MalI GATC 2 cut(s) 32, 338
MboI GATC 2 cut(s) 30, 336
MboII GAAGA 4 cut(s) 90, 221, 224, 295
MluCI AATT 2 cut(s) 391, 400
MnlI CCTC 4 cut(s) 87, 250, 376, 406
MseI TTAA 2 cut(s) 378, 458
MspR9I CCNGG 1 cut(s) 157
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 1 cut(s) 157
MwoI GCNNNNNNNGC 4 cut(s) 213, 287, 432, 441
NdeI CATATG 1 cut(s) 35
NdeII GATC 2 cut(s) 30, 336
NlaIII CATG 4 cut(s) 60, 190, 216, 252
NlaIV GGNNCC 1 cut(s) 295
NsbI TGCGCA 1 cut(s) 358
NspI RCATGY 3 cut(s) 60, 216, 252
PctI GAATGC 1 cut(s) 437
PkrI GCNGC 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 295
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 378, 458
SatI GCNGC 1 cut(s) 424
Sau3AI GATC 2 cut(s) 30, 336
ScrFI CCNGG 1 cut(s) 157
SetI ASST 4 cut(s) 98, 295, 368, 446
Sse9I AATT 2 cut(s) 391, 400
SspMI CTAG 1 cut(s) 22
StyD4I CCNGG 1 cut(s) 155
TasI AATT 2 cut(s) 391, 400
TatI WGTACW 1 cut(s) 209
Tru1I TTAA 2 cut(s) 378, 458
Tru9I TTAA 2 cut(s) 378, 458
TscAI CASTG 1 cut(s) 122
TseI GCWGC 1 cut(s) 423
TspDTI ATGAA 1 cut(s) 403
TspRI CASTG 1 cut(s) 122
XapI RAATTY 1 cut(s) 391
XceI RCATGY 3 cut(s) 60, 216, 252
XspI CTAG 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.