AT1G51610

metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000942
Physical Location & Seq
Forward (+)
19136214 .. 19139989
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G51610.1

Sequence Viewer

Length: 1374 bp
ATGCAATCATCGCATCGAATTTTGTCTCGCCTCCTTCATTCTCCTAGAAAAGGTTACATCAGAGCAAGTGTCGGTGGCTCTGTTCACTTTTTGGCGCTATTCGACGAGAAAGACAATGGATTTGTAGATACTACTCATCGGAGTTTTAGTTCTCTTATCCGTTCCAGTTCTCACGTAAGAGGGCTTATCTCCACCAATTGTTTAAATAAAGGTCTTGGTGTGCGATGTTCTGTTTCGCTTGATAGGGAAACACCTTTAATTGATACCTACTCCTCTCACCGGAATTTCTTCACTCGGGCGAAACAAGTGAAGAGAATTGAGATCAATGATCAACACAGTCAAAGAGCTGTCACTACTGCACTATGGTGCAACTTCCTCGTGTTTTCACTCAAGTTTGGGGTTTGGTGGACAAGTTCTAGCCATGTCATAATGGCTGAAGTAGTTCACTCCGTAGCTGATTTTGCTAACCAGGCACTTCTTGCTTATGGGCTCAGTAGCTCAAGGCGTGCCCCAGATGCTCTTCATCCCTATGGCTACTCAAAAGAAAGATTTGTATGGTCTTTGATATCTGCTGTTGGCATCTTTTGCCTTGGGTCTGGTGCTACCATAGTTAATGGAGTACAGAACTTGTGGACTTCTTCGCCACCTCCGAATATGGAATTAGCTGCTGTGGTAATTGGTGGTTCTTTTCTAATTGAAGGCGCTTCCTTACTCGTTGCAATTCAATCTGTCAAAAAAGGAGCTGCTCAAGAAGGCATGACCATAAGAGATTATATATGGCGTGGTCATGATCCTACTTCTGTTGCTGTTATGACTGAGGATGGCGCTGCAGTGGCAGGTTTGGCAATTGCTGCCGCATCTCTTGTCGCTGTTAGGATGACAGGAAATCCTATCTATGATCCTATAGGATCAATTGTAGTCGGAAATTTACTTGGAATGGTTGCTATATTTCTAATTCAACGAAACCGACATGCCTTGATTGGAAGAGCAATGGATGATCAAGACATGAGTAAAGTTCTTAAATTTTTGAGAAATGACTCGGTTGTAGATTCTTTATATGACTGCAAAAGTGAAGTGATTGGTCCTGGATCCTTCAGATTTAAAGCTGAAATAGATTTCAACGGGCAGATGGTTGTCCAAAACTATTTGAAGAGAACTGGGCGTGAAGAGTGGGCAAAAATGTTTCGCGAGGCTGCAAAGAACGGAGATGATTCTGCAATGCTCAATATTATGTCAAACTACGGTGAGGAAGTTGTCACGGCTTTAGGAAGCGAAGTAGACCGGTTAGAGAAAGAGATCCAAGAGCTTGTCCCAGGAATCCAGCATGTTGACATTGAAGCACACAACCCCACACCCACAGACCCATCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

457

Amino Acids

50.1

Weight (kDa)

7.24

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 118 - 326 1.1e-30 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 827
AccI GTMKAC 1 cut(s) 1278
AccII CGCG 1 cut(s) 1188
AciI CCGC 1 cut(s) 855
AclWI GGATC 6 cut(s) 785, 893, 916, 1083, 1096, 1291
AcsI RAATTY 4 cut(s) 18, 283, 925, 1022
AcuI CTGAAG 2 cut(s) 456, 1078
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 2 cut(s) 50, 279
AgeI ACCGGT 1 cut(s) 1281
AgsI TTSAA 6 cut(s) 698, 725, 959, 1120, 1150, 1337
AjnI CCWGG 3 cut(s) 468, 1084, 1312
AleI CACNNNNGTG 1 cut(s) 364
AloI GAACNNNNNNTCC 2 cut(s) 133, 165
AluBI AGCT 7 cut(s) 347, 455, 498, 665, 743, 1106, 1306
AluI AGCT 7 cut(s) 347, 455, 498, 665, 743, 1106, 1306
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 6 cut(s) 785, 893, 916, 1083, 1096, 1291
Ama87I CYCGRG 1 cut(s) 294
ApeKI GCWGC 5 cut(s) 665, 743, 827, 851, 1193
ApoI RAATTY 4 cut(s) 18, 283, 925, 1022
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AsiGI ACCGGT 1 cut(s) 1281
Asp700I GAANNNNTTC 2 cut(s) 287, 441
AspLEI GCGC 3 cut(s) 97, 704, 827
AspS9I GGNCC 1 cut(s) 1082
AsuHPI GGTGA 2 cut(s) 269, 1256
AvaI CYCGRG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 1082
BaeGI GKGCMC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 1088
BanII GRGCYC 1 cut(s) 492
BauI CACGAG 1 cut(s) 377
BbvI GCAGC 5 cut(s) 652, 730, 814, 838, 1180
BccI CCATC 3 cut(s) 815, 1123, 1372
BceAI ACGGC 1 cut(s) 1275
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 3 cut(s) 470, 1086, 1314
BclI TGATCA 2 cut(s) 328, 997
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 2 cut(s) 45, 417
BfmI CTRYAG 2 cut(s) 828, 903
BfoI RGCGCY 3 cut(s) 98, 705, 828
BfuAI ACCTGC 1 cut(s) 827
BisI GCNGC 6 cut(s) 666, 744, 828, 852, 855, 1194
BlsI GCNGC 6 cut(s) 667, 745, 829, 853, 856, 1195
Bme1390I CCNGG 3 cut(s) 470, 1086, 1314
Bme18I GGWCC 1 cut(s) 1082
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 1082
BmiI GGNNCC 1 cut(s) 1090
BmrFI CCNGG 3 cut(s) 470, 1086, 1314
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 4 cut(s) 22, 505, 588, 866
BmuI ACTGGG 1 cut(s) 1167
BpuEI CTTGAG 3 cut(s) 374, 484, 732
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 589, 1312
BsaWI WCCGGW 2 cut(s) 279, 1281
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 279
Bse118I RCCGGY 1 cut(s) 1281
Bse1I ACTGG 2 cut(s) 165, 1162
Bse3DI GCAATG 2 cut(s) 996, 1224
BseBI CCWGG 3 cut(s) 470, 1086, 1314
BseDI CCNNGG 2 cut(s) 589, 1312
BseGI GGATG 4 cut(s) 523, 826, 882, 1000
BseLI CCNNNNNNNGG 2 cut(s) 50, 279
BseMI GCAATG 2 cut(s) 996, 1224
BseMII CTCAG 2 cut(s) 505, 807
BseNI ACTGG 2 cut(s) 165, 1162
BseRI GAGGAG 1 cut(s) 262
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 5 cut(s) 652, 730, 814, 838, 1180
BsgI GTGCAG 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 1188
BshTI ACCGGT 1 cut(s) 1281
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 2 cut(s) 280, 1282
BslFI GGGAC 1 cut(s) 1295
BslI CCNNNNNNNGG 2 cut(s) 50, 279
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 1295
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 2 cut(s) 492, 511
Bsp143I GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
Bsp68I TCGCGA 1 cut(s) 1188
BspACI CCGC 1 cut(s) 855
BspCNI CTCAG 2 cut(s) 504, 808
BspFNI CGCG 1 cut(s) 1188
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 1 cut(s) 1090
BspMAI CTGCAG 1 cut(s) 832
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 6 cut(s) 785, 893, 916, 1083, 1096, 1291
BspQI GCTCTTC 2 cut(s) 525, 979
BsrDI GCAATG 2 cut(s) 996, 1224
BsrFI RCCGGY 1 cut(s) 1281
BsrI ACTGG 2 cut(s) 165, 1162
BssAI RCCGGY 1 cut(s) 1281
BssECI CCNNGG 2 cut(s) 589, 1312
BssMI GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
BssSI CACGAG 1 cut(s) 377
BssT1I CCWWGG 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 377
Bst2UI CCWGG 3 cut(s) 470, 1086, 1314
Bst4CI ACNGT 2 cut(s) 338, 1244
Bst6I CTCTTC 5 cut(s) 305, 525, 979, 1145, 1161
BstAPI GCANNNNNTGC 3 cut(s) 479, 585, 851
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 2 cut(s) 491, 816
BstENI CCTNNNNNAGG 1 cut(s) 48
BstF5I GGATG 4 cut(s) 523, 826, 882, 1000
BstFNI CGCG 1 cut(s) 1188
BstH2I RGCGCY 3 cut(s) 98, 705, 828
BstHHI GCGC 3 cut(s) 97, 704, 827
BstKTI GATC 8 cut(s) 324, 331, 793, 901, 911, 1000, 1091, 1299
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
BstMWI GCNNNNNNNGC 9 cut(s) 10, 461, 470, 479, 515, 585, 833, 842, 851
BstNI CCWGG 3 cut(s) 470, 1086, 1314
BstNSI RCATGY 2 cut(s) 974, 1328
BstSCI CCNGG 3 cut(s) 468, 1084, 1312
BstSFI CTRYAG 2 cut(s) 828, 903
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 1 cut(s) 1188
BstV1I GCAGC 5 cut(s) 652, 730, 814, 838, 1180
BstX2I RGATCY 2 cut(s) 1088, 1296
BstYI RGATCY 2 cut(s) 1088, 1296
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 4 cut(s) 523, 826, 882, 1000
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 1 cut(s) 837
BtuMI TCGCGA 1 cut(s) 1188
BveI ACCTGC 1 cut(s) 827
Cac8I GCNNGC 1 cut(s) 507
CciI TCATGA 1 cut(s) 787
CfoI GCGC 3 cut(s) 97, 704, 827
Cfr10I RCCGGY 1 cut(s) 1281
Cfr13I GGNCC 1 cut(s) 1082
Csp6I GTAC 1 cut(s) 620
CspAI ACCGGT 1 cut(s) 1281
CviAII CATG 6 cut(s) 422, 757, 788, 971, 1006, 1325
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 2 cut(s) 491, 816
DpnI GATC 8 cut(s) 323, 330, 792, 900, 910, 999, 1090, 1298
DpnII GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
DraI TTTAAA 2 cut(s) 204, 1102
Eam1104I CTCTTC 5 cut(s) 305, 525, 979, 1145, 1161
EarI CTCTTC 5 cut(s) 305, 525, 979, 1145, 1161
Eco130I CCWWGG 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 492
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 1082
Eco57I CTGAAG 2 cut(s) 456, 1078
Eco88I CYCGRG 1 cut(s) 294
EcoNI CCTNNNNNAGG 1 cut(s) 48
EcoRII CCWGG 3 cut(s) 468, 1084, 1312
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 589
FaeI CATG 6 cut(s) 425, 760, 791, 974, 1009, 1328
FaqI GGGAC 1 cut(s) 1295
FatI CATG 6 cut(s) 421, 756, 787, 970, 1005, 1324
FbaI TGATCA 2 cut(s) 328, 997
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 6 cut(s) 666, 744, 828, 852, 855, 1194
FokI GGATG 4 cut(s) 510, 833, 889, 1007
FriOI GRGCYC 1 cut(s) 492
Fsp4HI GCNGC 6 cut(s) 666, 744, 828, 852, 855, 1194
FspBI CTAG 2 cut(s) 45, 417
GlaI GCGC 3 cut(s) 96, 703, 826
GluI GCNGC 6 cut(s) 666, 744, 828, 852, 855, 1194
HaeII RGCGCY 3 cut(s) 98, 705, 828
HapII CCGG 2 cut(s) 280, 1282
HhaI GCGC 3 cut(s) 97, 704, 827
Hin1II CATG 6 cut(s) 425, 760, 791, 974, 1009, 1328
Hin6I GCGC 3 cut(s) 95, 702, 825
HinP1I GCGC 3 cut(s) 95, 702, 825
HincII GTYRAC 1 cut(s) 1330
HindII GTYRAC 1 cut(s) 1330
HinfI GANTC 4 cut(s) 1037, 1049, 1211, 1317
HpaII CCGG 2 cut(s) 280, 1282
HphI GGTGA 2 cut(s) 269, 1256
Hpy166II GTNNAC 6 cut(s) 85, 408, 445, 633, 1279, 1330
Hpy188I TCNGA 5 cut(s) 62, 141, 651, 923, 1097
Hpy188III TCNNGA 4 cut(s) 749, 788, 1001, 1187
Hpy8I GTNNAC 6 cut(s) 85, 408, 445, 633, 1279, 1330
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 44, 692, 746, 1102
HpyCH4III ACNGT 2 cut(s) 338, 1244
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 8 cut(s) 4, 359, 369, 719, 830, 1065, 1196, 1217
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 461, 470, 479, 515, 585, 833, 842, 851
HpyF3I CTNAG 2 cut(s) 491, 816
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 6 cut(s) 425, 760, 791, 974, 1009, 1328
HspAI GCGC 3 cut(s) 95, 702, 825
Ksp22I TGATCA 2 cut(s) 328, 997
Kzo9I GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
LguI GCTCTTC 2 cut(s) 525, 979
LmnI GCTCC 1 cut(s) 740
Lsp1109I GCAGC 5 cut(s) 652, 730, 814, 838, 1180
LweI GCATC 4 cut(s) 22, 505, 588, 866
MaeI CTAG 2 cut(s) 45, 417
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 53, 349, 1255
MalI GATC 8 cut(s) 323, 330, 792, 900, 910, 999, 1090, 1298
MboI GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
MboII GAAGA 7 cut(s) 280, 322, 512, 630, 996, 1162, 1178
MfeI CAATTG 3 cut(s) 196, 846, 912
MflI RGATCY 2 cut(s) 1088, 1296
MhlI GDGCHC 2 cut(s) 492, 511
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 1 cut(s) 901
MnlI CCTC 8 cut(s) 41, 173, 283, 386, 657, 811, 1183, 1240
MroXI GAANNNNTTC 2 cut(s) 287, 441
MseI TTAA 5 cut(s) 203, 257, 612, 1020, 1101
MslI CAYNNNNRTG 2 cut(s) 364, 528
MspI CCGG 2 cut(s) 280, 1282
MspR9I CCNGG 3 cut(s) 470, 1086, 1314
MunI CAATTG 3 cut(s) 196, 846, 912
MvaI CCWGG 3 cut(s) 470, 1086, 1314
MvnI CGCG 1 cut(s) 1188
MwoI GCNNNNNNNGC 9 cut(s) 10, 461, 470, 479, 515, 585, 833, 842, 851
NdeII GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
NlaIII CATG 6 cut(s) 425, 760, 791, 974, 1009, 1328
NlaIV GGNNCC 1 cut(s) 1090
NmuCI GTSAC 2 cut(s) 349, 1255
NruI TCGCGA 1 cut(s) 1188
NspI RCATGY 2 cut(s) 974, 1328
OliI CACNNNNGTG 1 cut(s) 364
PagI TCATGA 1 cut(s) 787
PciSI GCTCTTC 2 cut(s) 525, 979
PcsI WCGNNNNNNNCGW 1 cut(s) 647
PdmI GAANNNNTTC 2 cut(s) 287, 441
PfeI GAWTC 3 cut(s) 1049, 1211, 1317
PfoI TCCNGGA 1 cut(s) 1084
PinAI ACCGGT 1 cut(s) 1281
PkrI GCNGC 6 cut(s) 667, 745, 829, 853, 856, 1195
PleI GAGTC 1 cut(s) 1031
PpsI GAGTC 1 cut(s) 1031
Ppu21I YACGTR 1 cut(s) 175
Psp6I CCWGG 3 cut(s) 468, 1084, 1312
PspGI CCWGG 3 cut(s) 468, 1084, 1312
PspN4I GGNNCC 1 cut(s) 1090
PspPI GGNCC 1 cut(s) 1082
PstI CTGCAG 1 cut(s) 832
PsuI RGATCY 2 cut(s) 1088, 1296
RruI TCGCGA 1 cut(s) 1188
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
RseI CAYNNNNRTG 2 cut(s) 364, 528
SapI GCTCTTC 2 cut(s) 525, 979
SaqAI TTAA 5 cut(s) 203, 257, 612, 1020, 1101
SatI GCNGC 6 cut(s) 666, 744, 828, 852, 855, 1194
Sau3AI GATC 8 cut(s) 321, 328, 790, 898, 908, 997, 1088, 1296
Sau96I GGNCC 1 cut(s) 1082
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 3 cut(s) 470, 1086, 1314
SduI GDGCHC 2 cut(s) 492, 511
SfaNI GCATC 4 cut(s) 22, 505, 588, 866
SfcI CTRYAG 2 cut(s) 828, 903
SinI GGWCC 1 cut(s) 1082
SmiMI CAYNNNNRTG 2 cut(s) 364, 528
SmlI CTYRAG 3 cut(s) 389, 499, 747
SmoI CTYRAG 3 cut(s) 389, 499, 747
SsiI CCGC 1 cut(s) 855
SspI AATATT 1 cut(s) 1228
SspMI CTAG 2 cut(s) 45, 417
StyD4I CCNGG 3 cut(s) 468, 1084, 1312
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 2 cut(s) 338, 1244
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 16, 102
TatI WGTACW 1 cut(s) 619
TauI GCSGC 1 cut(s) 857
TfiI GAWTC 3 cut(s) 1049, 1211, 1317
Tru1I TTAA 5 cut(s) 203, 257, 612, 1020, 1101
Tru9I TTAA 5 cut(s) 203, 257, 612, 1020, 1101
TscAI CASTG 1 cut(s) 837
TseFI GTSAC 2 cut(s) 349, 1255
TseI GCWGC 5 cut(s) 665, 743, 827, 851, 1193
Tsp45I GTSAC 2 cut(s) 349, 1255
TspDTI ATGAA 2 cut(s) 26, 512
TspGWI ACGGA 3 cut(s) 149, 439, 1218
TspRI CASTG 1 cut(s) 837
VpaK11BI GGWCC 1 cut(s) 1082
XagI CCTNNNNNAGG 1 cut(s) 48
XapI RAATTY 4 cut(s) 18, 283, 925, 1022
XceI RCATGY 2 cut(s) 974, 1328
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 2 cut(s) 287, 441
XspI CTAG 2 cut(s) 45, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.