Rh2DG496100

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000942
Physical Location & Seq
Reverse (-)
71617005 .. 71625317
8313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG496100.1

Sequence Viewer

Length: 1023 bp
ATGAGAACGTCGTCGTTTTCTCTCCTCTCTCGCCTCCGCCACTCCCGCCGCTGCTGCTACTCCTCCTCTAACTCTCTGCTTTTCCACTTGGCCCATCAAAACGACGATCGCCGCTCCTCCCAAAACGCGCCGTTTAGCTCCTCCTCCTTTCACTCCTACTCCACTCACACTCACACTCAGTTGCAGCGGCTCTTCTCCTCAGCTGCCTCCTCCAAACGGTTCGTTTTGCTCGACTTGCTTCCTTCCCCCGACGCTGTTAATCACCACTCGTTGCATCGAAGTTTCTTCACCAGAGCTAAACCTGTTAAACAAGTCAATCTTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTAGTGTTTTCGCTCAAGTTCACAGTCTGGTTATCCACCTCCAGCCATGTAATGTTGGCTGAAGTTGTGCATTCCGTTGCTGATTTTGCTAATCAGGCACTTCTTGCTTATGGGTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTTACGGATACTCCAAGGAAAGATTTGTTTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCCGGTGCAACTATAGTTCATGGAGTTCAAAACTTGTGGGTTTCACAGCCCCCTCCAAATATTCAATATGCAGCTTTAGTGATTGGTGGTTCGTTTATTATTGAAGGCGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGGTGCTGCTGCTGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATGATCCTACCTCTGTTGCAGTCATGACTGAGGATGGCGCTGCTGTAGCTGGTCTTGCTATTGCTGCAGCATCATTGGTTGCTGTAAATGTCACAGGAAATGGAATTTACGATCCTATAGGTTCAATTGTAGTTGGCAACCTACTTGGAATGGTATGGACTGCTTCTTGTTATTCACAGTTATGTAGATACTGCAAGAGATTAGTACACTTCTGTTCCAAAGAAATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

340

Amino Acids

37.26

Weight (kDa)

9.42

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 119 - 315 3.1e-30 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 128
AciI CCGC 5 cut(s) 37, 46, 49, 112, 187
AclWI GGATC 2 cut(s) 788, 896
AcsI RAATTY 2 cut(s) 323, 894
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 1 cut(s) 996
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 6 cut(s) 323, 328, 626, 662, 701, 915
AluBI AGCT 7 cut(s) 138, 203, 296, 501, 671, 731, 839
AluI AGCT 7 cut(s) 138, 203, 296, 501, 671, 731, 839
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 1 cut(s) 765
Alw44I GTGCAC 1 cut(s) 510
AlwI GGATC 2 cut(s) 788, 896
AoxI GGCC 1 cut(s) 90
ApaLI GTGCAC 1 cut(s) 510
ApoI RAATTY 2 cut(s) 323, 894
AspLEI GCGC 3 cut(s) 130, 707, 830
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 2 cut(s) 254, 280
BaeGI GKGCMC 1 cut(s) 514
Bbv12I GWGCWC 1 cut(s) 514
BbvCI CCTCAGC 2 cut(s) 199, 753
BccI CCATC 2 cut(s) 102, 818
BceAI ACGGC 1 cut(s) 115
BciVI GTATCC 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 765
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 4 cut(s) 609, 834, 855, 906
BfoI RGCGCY 2 cut(s) 708, 831
BfuI GTATCC 1 cut(s) 530
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 598
BmsI GCATC 3 cut(s) 283, 508, 869
BpmI CTGGAG 1 cut(s) 403
Bpu10I CCTNAGC 2 cut(s) 199, 753
BpuEI CTTGAG 2 cut(s) 377, 487
BsaJI CCNNGG 1 cut(s) 543
BsaWI WCCGGW 1 cut(s) 599
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 524, 554
Bsc4I CCNNNNNNNGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMII CTCAG 4 cut(s) 191, 213, 744, 810
BseRI GAGGAG 8 cut(s) 14, 52, 55, 106, 130, 133, 187, 199
BseSI GKGCMC 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 1 cut(s) 92
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 514
BsiSI CCGG 1 cut(s) 600
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 3 cut(s) 106, 793, 901
BspACI CCGC 5 cut(s) 37, 46, 49, 112, 187
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 4 cut(s) 190, 212, 745, 811
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 2 cut(s) 790, 813
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 2 cut(s) 788, 896
BspQI GCTCTTC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 3 cut(s) 106, 793, 901
BssT1I CCWWGG 1 cut(s) 543
Bst4CI ACNGT 4 cut(s) 219, 341, 403, 969
Bst6I CTCTTC 2 cut(s) 197, 717
BstAPI GCANNNNNTGC 2 cut(s) 482, 518
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 4 cut(s) 177, 199, 753, 819
BstF5I GGATG 1 cut(s) 829
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 2 cut(s) 708, 831
BstHHI GCGC 3 cut(s) 130, 707, 830
BstKTI GATC 3 cut(s) 109, 796, 904
BstMAI GTCTC 1 cut(s) 765
BstMBI GATC 3 cut(s) 106, 793, 901
BstMCI CGRYCG 1 cut(s) 109
BstSFI CTRYAG 4 cut(s) 609, 834, 855, 906
BstSLI GKGCMC 1 cut(s) 514
BstUI CGCG 1 cut(s) 128
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 829
Cac8I GCNNGC 1 cut(s) 510
CciI TCATGA 2 cut(s) 790, 813
CfoI GCGC 3 cut(s) 130, 707, 830
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 995
CspCI CAANNNNNGTGG 2 cut(s) 614, 649
CviAII CATG 5 cut(s) 425, 617, 760, 791, 814
CviQI GTAC 1 cut(s) 995
DdeI CTNAG 4 cut(s) 177, 199, 753, 819
DpnI GATC 3 cut(s) 108, 795, 903
DpnII GATC 3 cut(s) 106, 793, 901
Eam1104I CTCTTC 2 cut(s) 197, 717
EarI CTCTTC 2 cut(s) 197, 717
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 543
Eco32I GATATC 1 cut(s) 570
Eco57I CTGAAG 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 323
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 5 cut(s) 428, 620, 763, 794, 817
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FatI CATG 5 cut(s) 424, 616, 759, 790, 813
FauI CCCGC 1 cut(s) 53
FokI GGATG 1 cut(s) 836
FspBI CTAG 1 cut(s) 380
GlaI GCGC 3 cut(s) 129, 706, 829
GsuI CTGGAG 1 cut(s) 403
HaeII RGCGCY 2 cut(s) 708, 831
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 600
HgaI GACGC 1 cut(s) 260
HhaI GCGC 3 cut(s) 130, 707, 830
Hin1II CATG 5 cut(s) 428, 620, 763, 794, 817
Hin6I GCGC 3 cut(s) 128, 705, 828
HinP1I GCGC 3 cut(s) 128, 705, 828
HpaII CCGG 1 cut(s) 600
HphI GGTGA 2 cut(s) 254, 280
Hpy166II GTNNAC 3 cut(s) 399, 512, 997
Hpy188III TCNNGA 4 cut(s) 320, 736, 791, 814
Hpy8I GTNNAC 3 cut(s) 399, 512, 997
Hpy99I CGWCG 4 cut(s) 13, 16, 107, 254
HpyAV CCTTC 2 cut(s) 252, 695
HpyCH4III ACNGT 4 cut(s) 219, 341, 403, 969
HpyCH4IV ACGT 1 cut(s) 8
HpyF3I CTNAG 4 cut(s) 177, 199, 753, 819
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 5 cut(s) 428, 620, 763, 794, 817
HspAI GCGC 3 cut(s) 128, 705, 828
Kzo9I GATC 3 cut(s) 106, 793, 901
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 2 cut(s) 119, 143
LweI GCATC 3 cut(s) 283, 508, 869
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 352, 880
MalI GATC 3 cut(s) 108, 795, 903
MbiI CCGCTC 1 cut(s) 114
MboI GATC 3 cut(s) 106, 793, 901
MboII GAAGA 3 cut(s) 184, 277, 704
MfeI CAATTG 1 cut(s) 915
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 4 cut(s) 323, 764, 894, 915
MseI TTAA 3 cut(s) 258, 306, 767
MslI CAYNNNNRTG 2 cut(s) 367, 970
MspA1I CMGCKG 3 cut(s) 51, 187, 203
MspI CCGG 1 cut(s) 600
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 448
MvnI CGCG 1 cut(s) 128
NdeII GATC 3 cut(s) 106, 793, 901
NlaIII CATG 5 cut(s) 428, 620, 763, 794, 817
NlaIV GGNNCC 1 cut(s) 598
NmuCI GTSAC 2 cut(s) 352, 880
PagI TCATGA 2 cut(s) 790, 813
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 448
Ple19I CGATCG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 1 cut(s) 91
PstI CTGCAG 1 cut(s) 859
PvuI CGATCG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 996
RsaNI GTAC 1 cut(s) 995
RseI CAYNNNNRTG 2 cut(s) 367, 970
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 258, 306, 767
Sau3AI GATC 3 cut(s) 106, 793, 901
Sau96I GGNCC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 514
SfaNI GCATC 3 cut(s) 283, 508, 869
SfcI CTRYAG 4 cut(s) 609, 834, 855, 906
SmiMI CAYNNNNRTG 2 cut(s) 367, 970
SmlI CTYRAG 2 cut(s) 392, 502
SmoI CTYRAG 2 cut(s) 392, 502
Sse9I AATT 4 cut(s) 323, 764, 894, 915
SsiI CCGC 5 cut(s) 37, 46, 49, 112, 187
SspI AATATT 1 cut(s) 658
SspMI CTAG 1 cut(s) 380
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 4 cut(s) 219, 341, 403, 969
TaiI ACGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 231, 277
TasI AATT 4 cut(s) 323, 764, 894, 915
TatI WGTACW 1 cut(s) 994
TauI GCSGC 3 cut(s) 51, 114, 190
Tru1I TTAA 3 cut(s) 258, 306, 767
Tru9I TTAA 3 cut(s) 258, 306, 767
TseFI GTSAC 2 cut(s) 352, 880
Tsp45I GTSAC 2 cut(s) 352, 880
TspDTI ATGAA 2 cut(s) 605, 776
TspGWI ACGGA 2 cut(s) 442, 549
VneI GTGCAC 1 cut(s) 510
XapI RAATTY 2 cut(s) 323, 894
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.