pycom08g05510

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000942
Physical Location & Seq
Reverse (-)
4218080 .. 4218718
639 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05510.1

Sequence Viewer

Length: 639 bp
ATGGATAGCACATTAGCACTACCAACTGCTTCAATCTTAATTCTCTTGTTTGATGGAATGTCTAAGAGACATAATTACAACTGGAATTCTCGTAGCTGCTCATATGCTGTTTGGAATGCTCCTATGCTATGTGGAATGCACAAACTTATGCATCATTTGGTGTTGCAAATTCATGACCATGATTGGCACCCCATTGGTGATAAGAAGTCAATTAAAGTAGAAGTTAAAGTGTCTGTGGGAATTGGTCATGGAGGGTACACTATTCTTTGGTGGGTGCTGTGGAGGATGAAAGCTATAGGAATCACAACTTCTTTTGCAAAAGCACCAATGGAATTTGTTCATGGAGTTCAAAACTTGTGGACTTCACTGCCTCCTCCAAAAATTCGGTATGCAGCTCTTGTGATTGGTACCTCATTTACCATTGAAGGTGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGGAAGGTGCAGCTGCTGAGGGCATGAAGTTGACAGACTTTATATGGCGCGGACATGATCCCACATCTGTTTCAGTCTTGACGGAGGATGGCGCAGCTGTAACTGGTCTTGCTATTGCTGTGGCATCATTGGTTGCAGTGAATAGAACAGGAAATGCACTTTATTGGAAAATATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

213

Amino Acids

23.28

Weight (kDa)

9.1

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 407
AccB1I GGYRCC 2 cut(s) 186, 407
AccII CGCG 1 cut(s) 510
AciI CCGC 1 cut(s) 510
AclWI GGATC 1 cut(s) 512
AcsI RAATTY 4 cut(s) 85, 168, 332, 381
AfaI GTAC 2 cut(s) 257, 409
AgsI TTSAA 3 cut(s) 33, 350, 425
AluBI AGCT 6 cut(s) 96, 293, 395, 455, 473, 557
AluI AGCT 6 cut(s) 96, 293, 395, 455, 473, 557
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 1 cut(s) 512
AlwNI CAGNNNCTG 1 cut(s) 476
ApeKI GCWGC 5 cut(s) 96, 392, 470, 473, 554
ApoI RAATTY 4 cut(s) 85, 168, 332, 381
Asp700I GAANNNNTTC 1 cut(s) 336
Asp718I GGTACC 1 cut(s) 407
AspLEI GCGC 2 cut(s) 510, 554
AsuHPI GGTGA 1 cut(s) 209
BanI GGYRCC 2 cut(s) 186, 407
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 83, 404, 460, 482, 566
BccI CCATC 2 cut(s) 47, 542
BcoDI GTCTC 1 cut(s) 61
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 5 cut(s) 97, 393, 471, 474, 555
BlsI GCNGC 5 cut(s) 98, 394, 472, 475, 556
BmiI GGNNCC 2 cut(s) 188, 409
BmsI GCATC 2 cut(s) 160, 593
Bpu10I CCTNAGC 1 cut(s) 477
Bse1I ACTGG 2 cut(s) 86, 568
BseGI GGATG 2 cut(s) 291, 553
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 2 cut(s) 86, 568
BseRI GAGGAG 1 cut(s) 363
BseXI GCAGC 5 cut(s) 83, 404, 460, 482, 566
BsgI GTGCAG 1 cut(s) 489
Bsh1236I CGCG 1 cut(s) 510
BshNI GGYRCC 2 cut(s) 186, 407
BsmAI GTCTC 1 cut(s) 61
BsmI GAATGC 2 cut(s) 121, 141
Bsp143I GATC 1 cut(s) 517
BspACI CCGC 1 cut(s) 510
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 188, 409
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 2 cut(s) 186, 407
BsrI ACTGG 2 cut(s) 86, 568
BssMI GATC 1 cut(s) 517
Bst6I CTCTTC 1 cut(s) 441
BstDEI CTNAG 2 cut(s) 63, 477
BstF5I GGATG 2 cut(s) 291, 553
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 2 cut(s) 510, 554
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 5 cut(s) 83, 404, 460, 482, 566
BtsCI GGATG 2 cut(s) 291, 553
BtsI GCAGTG 2 cut(s) 365, 603
BtsIMutI CAGTG 2 cut(s) 365, 603
CaiI CAGNNNCTG 1 cut(s) 476
CciI TCATGA 1 cut(s) 172
CfoI GCGC 2 cut(s) 510, 554
Csp6I GTAC 2 cut(s) 256, 408
CspCI CAANNNNNGTGG 2 cut(s) 338, 373
CviAII CATG 6 cut(s) 173, 179, 248, 341, 484, 515
CviJI RGCY 6 cut(s) 96, 293, 395, 455, 473, 557
CviKI_1 RGCY 6 cut(s) 96, 293, 395, 455, 473, 557
CviQI GTAC 2 cut(s) 256, 408
DdeI CTNAG 2 cut(s) 63, 477
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 153
FaeI CATG 6 cut(s) 176, 182, 251, 344, 487, 518
FatI CATG 6 cut(s) 172, 178, 247, 340, 483, 514
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 5 cut(s) 97, 393, 471, 474, 555
FokI GGATG 2 cut(s) 298, 560
Fsp4HI GCNGC 5 cut(s) 97, 393, 471, 474, 555
GlaI GCGC 2 cut(s) 509, 553
GluI GCNGC 5 cut(s) 97, 393, 471, 474, 555
HhaI GCGC 2 cut(s) 510, 554
Hin1II CATG 6 cut(s) 176, 182, 251, 344, 487, 518
Hin6I GCGC 2 cut(s) 508, 552
HinP1I GCGC 2 cut(s) 508, 552
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HinfI GANTC 1 cut(s) 300
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 3 cut(s) 258, 360, 492
Hpy188III TCNNGA 2 cut(s) 173, 538
Hpy8I GTNNAC 3 cut(s) 258, 360, 492
HpyAV CCTTC 2 cut(s) 419, 458
HpyCH4V TGCA 8 cut(s) 139, 151, 166, 317, 392, 470, 596, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 2 cut(s) 63, 477
Hsp92II CATG 6 cut(s) 176, 182, 251, 344, 487, 518
HspAI GCGC 2 cut(s) 508, 552
KpnI GGTACC 1 cut(s) 411
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 3 cut(s) 67, 549, 594
Lsp1109I GCAGC 5 cut(s) 83, 404, 460, 482, 566
LweI GCATC 2 cut(s) 160, 593
MaeIII GTNAC 1 cut(s) 559
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 1 cut(s) 428
MluCI AATT 8 cut(s) 39, 73, 85, 168, 210, 240, 332, 381
MnlI CCTC 7 cut(s) 245, 276, 381, 384, 421, 472, 538
Mph1103I ATGCAT 1 cut(s) 153
MroXI GAANNNNTTC 1 cut(s) 336
MseI TTAA 3 cut(s) 38, 213, 225
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 2 cut(s) 473, 557
Mva1269I GAATGC 2 cut(s) 121, 141
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 1 cut(s) 452
NdeI CATATG 1 cut(s) 103
NdeII GATC 1 cut(s) 517
NlaIII CATG 6 cut(s) 176, 182, 251, 344, 487, 518
NlaIV GGNNCC 2 cut(s) 188, 409
NsiI ATGCAT 1 cut(s) 153
PagI TCATGA 1 cut(s) 172
PctI GAATGC 2 cut(s) 121, 141
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 1 cut(s) 300
PkrI GCNGC 5 cut(s) 98, 394, 472, 475, 556
PspN4I GGNNCC 2 cut(s) 188, 409
PstNI CAGNNNCTG 1 cut(s) 476
PvuII CAGCTG 2 cut(s) 473, 557
RsaI GTAC 2 cut(s) 257, 409
RsaNI GTAC 2 cut(s) 256, 408
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 3 cut(s) 38, 213, 225
SatI GCNGC 5 cut(s) 97, 393, 471, 474, 555
Sau3AI GATC 1 cut(s) 517
SetI ASST 9 cut(s) 98, 295, 397, 413, 430, 457, 469, 475, 559
SfaNI GCATC 2 cut(s) 160, 593
SfcI CTRYAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 8 cut(s) 39, 73, 85, 168, 210, 240, 332, 381
SsiI CCGC 1 cut(s) 510
SspI AATATT 1 cut(s) 633
TasI AATT 8 cut(s) 39, 73, 85, 168, 210, 240, 332, 381
TfiI GAWTC 1 cut(s) 300
Tru1I TTAA 3 cut(s) 38, 213, 225
Tru9I TTAA 3 cut(s) 38, 213, 225
TscAI CASTG 2 cut(s) 372, 603
TseI GCWGC 5 cut(s) 96, 392, 470, 473, 554
TspDTI ATGAA 4 cut(s) 161, 302, 329, 500
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 2 cut(s) 372, 603
XapI RAATTY 4 cut(s) 85, 168, 332, 381
XmnI GAANNNNTTC 1 cut(s) 336
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.