Rh5DG280300

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000942
Physical Location & Seq
Reverse (-)
34331231 .. 34333130
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG280300.1

Sequence Viewer

Length: 435 bp
ATGAGTTGTGGTGCAGTCGGCGAGCGAAGATGCAAACACGGTGTATGGTGCATTGCAGGGGTTAGAGGATGCCTTTGTTCTGGTGCAACTATAGTTCATGGAGTTCAAAACTTGTGGGTTTCACAGCCCCCTCCAAATATTCAATATGTAGATATAGTGATTGGTGGTTCGTTTATTATTGAAGGCGCTTCTCTTCATGTTGCCATACAAGCTGTCAAGAAAGGTGCTGCTGCTGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATTATCCTACCTCTGTTGCAGTCATGACTGAGGATGGCGCTGCTGTAGCTGGTCTTGCTATTGTTGCAGCATCATTGGTTGCTGTAAATGTCACAGGAAATGGAATTTACGATCCTATAGGTTCATTTGTAGTTGGCAAGGATCTTGGAATGGTGGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

144

Amino Acids

14.85

Weight (kDa)

7.66

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 377, 420
AcsI RAATTY 1 cut(s) 375
AgsI TTSAA 3 cut(s) 107, 143, 182
AluBI AGCT 2 cut(s) 212, 320
AluI AGCT 2 cut(s) 212, 320
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 2 cut(s) 377, 420
ApeKI GCWGC 4 cut(s) 227, 230, 311, 338
ApoI RAATTY 1 cut(s) 375
AspLEI GCGC 2 cut(s) 188, 311
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 214, 217, 298, 350
BccI CCATC 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 246
BfmI CTRYAG 3 cut(s) 90, 315, 387
BfoI RGCGCY 2 cut(s) 189, 312
BisI GCNGC 4 cut(s) 228, 231, 312, 339
BlsI GCNGC 4 cut(s) 229, 232, 313, 340
BmsI GCATC 3 cut(s) 20, 59, 350
Bpu10I CCTNAGC 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 51
BseGI GGATG 2 cut(s) 74, 310
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 2 cut(s) 225, 291
BseXI GCAGC 4 cut(s) 214, 217, 298, 350
BsgI GTGCAG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 246
Bsp143I GATC 2 cut(s) 382, 412
BspCNI CTCAG 2 cut(s) 226, 292
BspHI TCATGA 1 cut(s) 294
BspPI GGATC 2 cut(s) 377, 420
BsrDI GCAATG 1 cut(s) 51
BssMI GATC 2 cut(s) 382, 412
Bst4CI ACNGT 1 cut(s) 41
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 234, 300
BstF5I GGATG 2 cut(s) 74, 310
BstH2I RGCGCY 2 cut(s) 189, 312
BstHHI GCGC 2 cut(s) 188, 311
BstKTI GATC 2 cut(s) 385, 415
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 2 cut(s) 382, 412
BstMWI GCNNNNNNNGC 4 cut(s) 209, 317, 326, 335
BstSFI CTRYAG 3 cut(s) 90, 315, 387
BstV1I GCAGC 4 cut(s) 214, 217, 298, 350
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BtsCI GGATG 2 cut(s) 74, 310
Cac8I GCNNGC 1 cut(s) 23
CciI TCATGA 1 cut(s) 294
CfoI GCGC 2 cut(s) 188, 311
CspCI CAANNNNNGTGG 2 cut(s) 95, 130
CviAII CATG 4 cut(s) 98, 197, 241, 295
CviJI RGCY 4 cut(s) 127, 212, 320, 428
CviKI_1 RGCY 4 cut(s) 127, 212, 320, 428
DdeI CTNAG 2 cut(s) 234, 300
DpnI GATC 2 cut(s) 384, 414
DpnII GATC 2 cut(s) 382, 412
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
FaeI CATG 4 cut(s) 101, 200, 244, 298
FatI CATG 4 cut(s) 97, 196, 240, 294
Fnu4HI GCNGC 4 cut(s) 228, 231, 312, 339
FokI GGATG 2 cut(s) 81, 317
Fsp4HI GCNGC 4 cut(s) 228, 231, 312, 339
GlaI GCGC 2 cut(s) 187, 310
GluI GCNGC 4 cut(s) 228, 231, 312, 339
HaeII RGCGCY 2 cut(s) 189, 312
HhaI GCGC 2 cut(s) 188, 311
Hin1II CATG 4 cut(s) 101, 200, 244, 298
Hin6I GCGC 2 cut(s) 186, 309
HinP1I GCGC 2 cut(s) 186, 309
Hpy188I TCNGA 1 cut(s) 434
Hpy188III TCNNGA 2 cut(s) 217, 295
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4V TGCA 7 cut(s) 14, 33, 51, 56, 86, 290, 338
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 317, 326, 335
HpyF3I CTNAG 2 cut(s) 234, 300
Hsp92II CATG 4 cut(s) 101, 200, 244, 298
HspAI GCGC 2 cut(s) 186, 309
Kzo9I GATC 2 cut(s) 382, 412
LpnPI CCDG 4 cut(s) 42, 66, 306, 351
Lsp1109I GCAGC 4 cut(s) 214, 217, 298, 350
LweI GCATC 3 cut(s) 20, 59, 350
MaeIII GTNAC 1 cut(s) 361
MalI GATC 2 cut(s) 384, 414
MboI GATC 2 cut(s) 382, 412
MboII GAAGA 2 cut(s) 39, 185
MflI RGATCY 1 cut(s) 412
MluCI AATT 2 cut(s) 245, 375
MnlI CCTC 5 cut(s) 59, 141, 229, 292, 295
MseI TTAA 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 209, 317, 326, 335
NdeII GATC 2 cut(s) 382, 412
NlaIII CATG 4 cut(s) 101, 200, 244, 298
NmuCI GTSAC 1 cut(s) 361
PagI TCATGA 1 cut(s) 294
PkrI GCNGC 4 cut(s) 229, 232, 313, 340
PsuI RGATCY 1 cut(s) 412
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 4 cut(s) 228, 231, 312, 339
Sau3AI GATC 2 cut(s) 382, 412
SetI ASST 5 cut(s) 214, 226, 284, 322, 394
SfaNI GCATC 3 cut(s) 20, 59, 350
SfcI CTRYAG 3 cut(s) 90, 315, 387
Sse9I AATT 2 cut(s) 245, 375
SspI AATATT 1 cut(s) 139
TaaI ACNGT 1 cut(s) 41
TasI AATT 2 cut(s) 245, 375
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 227, 230, 311, 338
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 4 cut(s) 86, 185, 257, 384
XapI RAATTY 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.