Rmu_sc0018143.1_g000001

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018143.1
OG0000942
Physical Location & Seq
Reverse (-)
1022 .. 5190
4169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018143.1_g000001.1.cds

Sequence Viewer

Length: 1362 bp
atgagaacgtcgtcgttttctctcctctctcgcctccgccactcccgccgccgctgctactcctctaaccctctgcttttccgcttggcccatcaaaacgacgatcgccgctcctcccaaaacgcgccgtttagctcctcctcctttcactcctactccactcacactcagttgcagcggctcttctcctcagctgcctcctccaaacggttcgttttgctcgacctgcttccttcccccggcgctgttaatcaccactcgttgcatcgacgtttcttcaccagagctaaacctgttaaacaagtcaatcttgaattcaatgaccagcacagtcaaagggcagtcacaactgcattgtggtgcaacttcctagtgttttcgctcaagttcacagtctggttatccacctccagccatgtaatgttggctgaagttgtgcattccgttgctgattttgctaatcaggcacttcttgcttatgggttgagtagctcaaggcgtgcaccagatgctctccacccttacggatactccaaggaaagatttgtttggtctttgatatctgctgttggtatcttttgtcttggttccggtgcaactatagttcatggagttcaaaacttgtgggtttcacagccccctccaaatattcaatatgcagctttagtgattggtggttcgtttattattgaaggcgcttctcttcttgttgccatacaagctgtcaagaaaggtgctgctgctgagggcatgaaattaagagactatatatggcgtggtcatgatcctacctctgttgcagtcatgactgaggatggcgctgctgtagctggtcttgctattgctgcagcatcattggttgctgtaaatgtcacaggaaatggaatttacgatcctataggttcaattgtagttggcaacctacttggaatggtggctatctttctgatacaaaggaatcgacatgctttaattggtagggcaatggatgactatgatgtagagaaggttcttcagttcttgaaaaatgacccggttgtagattctttatatgattgcaaaagcgaggtgattggcccgggattcttcagatttaaggctgagatagatttcaatggagtggtggtagttcaaaattacctctataggactggacgtcaagaatgggcaagacagtttcgtgaagctgcaaaggagaaggatgatgctgctttgctgaaaatgatgtccaattatggtgaagaagtagttacagccttgggaagtgaggttgatagattggaaaaggagattcaagagcttgttcctggtatacggcatgtcgacattgaggcacacaatccaatagagctgtccacgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

453

Amino Acids

50.15

Weight (kDa)

8.91

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1159
Acc36I ACCTGC 1 cut(s) 234
AccBSI CCGCTC 1 cut(s) 111
AccI GTMKAC 2 cut(s) 1312, 1323
AccII CGCG 1 cut(s) 125
AciI CCGC 7 cut(s) 37, 46, 49, 52, 82, 109, 178
AclWI GGATC 2 cut(s) 779, 887
AcsI RAATTY 2 cut(s) 314, 885
AcuI CTGAAG 3 cut(s) 450, 998, 1072
AcvI CACGTG 1 cut(s) 1359
AcyI GRCGYC 1 cut(s) 1156
AfiI CCNNNNNNNGG 2 cut(s) 207, 239
AhdI GACNNNNNGTC 1 cut(s) 1155
AjnI CCWGG 1 cut(s) 1306
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 756
Alw44I GTGCAC 1 cut(s) 501
AlwI GGATC 2 cut(s) 779, 887
Ama87I CYCGRG 1 cut(s) 1078
AoxI GGCC 2 cut(s) 87, 1075
ApaLI GTGCAC 1 cut(s) 501
ApoI RAATTY 2 cut(s) 314, 885
AspLEI GCGC 4 cut(s) 127, 245, 698, 821
AspS9I GGNCC 2 cut(s) 88, 1076
AsuC2I CCSGG 4 cut(s) 240, 1034, 1079, 1080
AsuHPI GGTGA 4 cut(s) 245, 271, 1081, 1250
AvaI CYCGRG 1 cut(s) 1078
BaeGI GKGCMC 1 cut(s) 505
BbrPI CACGTG 1 cut(s) 1359
Bbv12I GWGCWC 1 cut(s) 505
BbvCI CCTCAGC 2 cut(s) 190, 744
BccI CCATC 2 cut(s) 99, 809
BceAI ACGGC 2 cut(s) 112, 1331
BciT130I CCWGG 1 cut(s) 1308
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 4 cut(s) 240, 1034, 1079, 1080
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 5 cut(s) 600, 825, 846, 897, 1144
BfoI RGCGCY 3 cut(s) 246, 699, 822
BfuAI ACCTGC 1 cut(s) 234
BfuI GTATCC 1 cut(s) 521
Bme1390I CCNGG 5 cut(s) 240, 1034, 1079, 1080, 1308
BmeRI GACNNNNNGTC 1 cut(s) 1155
BmeT110I CYCGRG 1 cut(s) 1078
BmgT120I GGNCC 2 cut(s) 88, 1076
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 5 cut(s) 240, 1034, 1079, 1080, 1308
BmsI GCATC 4 cut(s) 274, 499, 860, 1195
BpmI CTGGAG 1 cut(s) 394
Bpu10I CCTNAGC 2 cut(s) 190, 744
BpuEI CTTGAG 2 cut(s) 368, 478
BpuMI CCSGG 4 cut(s) 240, 1034, 1079, 1080
BsaAI YACGTR 1 cut(s) 1359
BsaHI GRCGYC 1 cut(s) 1156
BsaJI CCNNGG 4 cut(s) 238, 534, 1078, 1257
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 515, 545, 1110, 1140
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 239
Bse1I ACTGG 1 cut(s) 1156
Bse3DI GCAATG 1 cut(s) 990
BseBI CCWGG 1 cut(s) 1308
BseDI CCNNGG 4 cut(s) 238, 534, 1078, 1257
BseGI GGATG 3 cut(s) 820, 994, 1207
BseLI CCNNNNNNNGG 2 cut(s) 207, 239
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 5 cut(s) 182, 204, 735, 801, 1092
BseNI ACTGG 1 cut(s) 1156
BseRI GAGGAG 7 cut(s) 14, 52, 103, 127, 130, 178, 190
BseSI GKGCMC 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 89, 1077
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 1078
BsiSI CCGG 4 cut(s) 240, 591, 1034, 1079
BslI CCNNNNNNNGG 2 cut(s) 207, 239
BsmAI GTCTC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 89, 1077
BsoBI CYCGRG 1 cut(s) 1078
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 3 cut(s) 103, 784, 892
BspACI CCGC 7 cut(s) 37, 46, 49, 52, 82, 109, 178
BspANI GGCC 2 cut(s) 89, 1077
BspCNI CTCAG 5 cut(s) 181, 203, 736, 802, 1093
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 2 cut(s) 781, 804
BspLI GGNNCC 1 cut(s) 589
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 2 cut(s) 779, 887
BspQI GCTCTTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 990
BsrI ACTGG 1 cut(s) 1156
BssECI CCNNGG 4 cut(s) 238, 534, 1078, 1257
BssMI GATC 3 cut(s) 103, 784, 892
BssNAI GTATAC 1 cut(s) 1313
BssNI GRCGYC 1 cut(s) 1156
BssT1I CCWWGG 2 cut(s) 534, 1257
Bst1107I GTATAC 1 cut(s) 1313
Bst2UI CCWGG 1 cut(s) 1308
Bst4CI ACNGT 4 cut(s) 210, 332, 394, 1176
Bst6I CTCTTC 2 cut(s) 188, 708
BstACI GRCGYC 1 cut(s) 1156
BstAPI GCANNNNNTGC 2 cut(s) 473, 509
BstBAI YACGTR 1 cut(s) 1359
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 5 cut(s) 168, 190, 744, 810, 1101
BstF5I GGATG 3 cut(s) 820, 994, 1207
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 3 cut(s) 246, 699, 822
BstHHI GCGC 4 cut(s) 127, 245, 698, 821
BstKTI GATC 3 cut(s) 106, 787, 895
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 3 cut(s) 103, 784, 892
BstMCI CGRYCG 1 cut(s) 106
BstNI CCWGG 1 cut(s) 1308
BstNSI RCATGY 2 cut(s) 968, 1322
BstSCI CCNGG 5 cut(s) 238, 1032, 1077, 1078, 1306
BstSFI CTRYAG 5 cut(s) 600, 825, 846, 897, 1144
BstSLI GKGCMC 1 cut(s) 505
BstUI CGCG 1 cut(s) 125
BstZ17I GTATAC 1 cut(s) 1313
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 2 cut(s) 89, 1077
BtsCI GGATG 3 cut(s) 820, 994, 1207
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 501
CciI TCATGA 2 cut(s) 781, 804
CfoI GCGC 4 cut(s) 127, 245, 698, 821
Cfr13I GGNCC 2 cut(s) 88, 1076
Cfr9I CCCGGG 1 cut(s) 1078
CspCI CAANNNNNGTGG 2 cut(s) 605, 640
CviAII CATG 7 cut(s) 416, 608, 751, 782, 805, 965, 1319
DdeI CTNAG 5 cut(s) 168, 190, 744, 810, 1101
DpnI GATC 3 cut(s) 105, 786, 894
DpnII GATC 3 cut(s) 103, 784, 892
DriI GACNNNNNGTC 1 cut(s) 1155
Eam1104I CTCTTC 2 cut(s) 188, 708
Eam1105I GACNNNNNGTC 1 cut(s) 1155
EarI CTCTTC 2 cut(s) 188, 708
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 534, 1257
Eco32I GATATC 1 cut(s) 561
Eco57I CTGAAG 3 cut(s) 450, 998, 1072
Eco72I CACGTG 1 cut(s) 1359
Eco88I CYCGRG 1 cut(s) 1078
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 1306
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 2 cut(s) 534, 1257
ErhI CCWWGG 2 cut(s) 534, 1257
FaeI CATG 7 cut(s) 419, 611, 754, 785, 808, 968, 1322
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 7 cut(s) 415, 607, 750, 781, 804, 964, 1318
FauI CCCGC 1 cut(s) 53
FblI GTMKAC 2 cut(s) 1312, 1323
FokI GGATG 3 cut(s) 827, 1001, 1214
FspBI CTAG 1 cut(s) 371
GlaI GCGC 4 cut(s) 126, 244, 697, 820
GsuI CTGGAG 1 cut(s) 394
HaeII RGCGCY 3 cut(s) 246, 699, 822
HaeIII GGCC 2 cut(s) 89, 1077
HapII CCGG 4 cut(s) 240, 591, 1034, 1079
HhaI GCGC 4 cut(s) 127, 245, 698, 821
Hin1I GRCGYC 1 cut(s) 1156
Hin1II CATG 7 cut(s) 419, 611, 754, 785, 808, 968, 1322
Hin6I GCGC 4 cut(s) 125, 243, 696, 819
HinP1I GCGC 4 cut(s) 125, 243, 696, 819
HincII GTYRAC 1 cut(s) 1324
HindII GTYRAC 1 cut(s) 1324
HinfI GANTC 4 cut(s) 958, 1043, 1083, 1291
HpaII CCGG 4 cut(s) 240, 591, 1034, 1079
HphI GGTGA 4 cut(s) 245, 271, 1081, 1250
Hpy166II GTNNAC 5 cut(s) 390, 503, 1313, 1324, 1356
Hpy188I TCNGA 2 cut(s) 948, 1091
Hpy188III TCNNGA 8 cut(s) 311, 727, 782, 805, 1021, 1160, 1181, 1295
Hpy8I GTNNAC 5 cut(s) 390, 503, 1313, 1324, 1356
Hpy99I CGWCG 4 cut(s) 13, 16, 104, 273
HpyAV CCTTC 4 cut(s) 243, 686, 1000, 1192
HpyCH4III ACNGT 4 cut(s) 210, 332, 394, 1176
HpyCH4IV ACGT 4 cut(s) 8, 271, 1156, 1358
HpyF3I CTNAG 5 cut(s) 168, 190, 744, 810, 1101
HpySE526I ACGT 4 cut(s) 8, 271, 1156, 1358
Hsp92I GRCGYC 1 cut(s) 1156
Hsp92II CATG 7 cut(s) 419, 611, 754, 785, 808, 968, 1322
HspAI GCGC 4 cut(s) 125, 243, 696, 819
Kzo9I GATC 3 cut(s) 103, 784, 892
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 2 cut(s) 116, 140
LweI GCATC 4 cut(s) 274, 499, 860, 1195
MaeI CTAG 1 cut(s) 371
MaeII ACGT 4 cut(s) 8, 271, 1156, 1358
MaeIII GTNAC 3 cut(s) 343, 871, 1249
MalI GATC 3 cut(s) 105, 786, 894
MbiI CCGCTC 1 cut(s) 111
MboI GATC 3 cut(s) 103, 784, 892
MboII GAAGA 6 cut(s) 175, 268, 695, 1004, 1078, 1253
MfeI CAATTG 1 cut(s) 906
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 7 cut(s) 314, 755, 885, 906, 972, 1135, 1231
MseI TTAA 5 cut(s) 249, 297, 758, 971, 1095
MslI CAYNNNNRTG 1 cut(s) 358
MspA1I CMGCKG 3 cut(s) 54, 178, 194
MspI CCGG 4 cut(s) 240, 591, 1034, 1079
MspR9I CCNGG 5 cut(s) 240, 1034, 1079, 1080, 1308
MunI CAATTG 1 cut(s) 906
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 1 cut(s) 1308
MvnI CGCG 1 cut(s) 125
NciI CCSGG 4 cut(s) 240, 1034, 1079, 1080
NdeII GATC 3 cut(s) 103, 784, 892
NlaIII CATG 7 cut(s) 419, 611, 754, 785, 808, 968, 1322
NlaIV GGNNCC 1 cut(s) 589
NmuCI GTSAC 2 cut(s) 343, 871
NspI RCATGY 2 cut(s) 968, 1322
PagI TCATGA 2 cut(s) 781, 804
PciSI GCTCTTC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 4 cut(s) 958, 1043, 1083, 1291
Ple19I CGATCG 1 cut(s) 106
PmaCI CACGTG 1 cut(s) 1359
PmlI CACGTG 1 cut(s) 1359
Ppu21I YACGTR 1 cut(s) 1359
Psp6I CCWGG 1 cut(s) 1306
PspCI CACGTG 1 cut(s) 1359
PspGI CCWGG 1 cut(s) 1306
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 2 cut(s) 88, 1076
PsrI GAACNNNNNNTAC 2 cut(s) 993, 1025
PstI CTGCAG 1 cut(s) 850
PvuI CGATCG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 358
SalI GTCGAC 1 cut(s) 1322
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 5 cut(s) 249, 297, 758, 971, 1095
Sau3AI GATC 3 cut(s) 103, 784, 892
Sau96I GGNCC 2 cut(s) 88, 1076
ScrFI CCNGG 5 cut(s) 240, 1034, 1079, 1080, 1308
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 4 cut(s) 274, 499, 860, 1195
SfcI CTRYAG 5 cut(s) 600, 825, 846, 897, 1144
SmaI CCCGGG 1 cut(s) 1080
SmiMI CAYNNNNRTG 1 cut(s) 358
SmlI CTYRAG 2 cut(s) 383, 493
SmoI CTYRAG 2 cut(s) 383, 493
Sse9I AATT 7 cut(s) 314, 755, 885, 906, 972, 1135, 1231
SsiI CCGC 7 cut(s) 37, 46, 49, 52, 82, 109, 178
SspI AATATT 1 cut(s) 649
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 5 cut(s) 238, 1032, 1077, 1078, 1306
StyI CCWWGG 2 cut(s) 534, 1257
TaaI ACNGT 4 cut(s) 210, 332, 394, 1176
TaiI ACGT 4 cut(s) 11, 274, 1159, 1361
TaqI TCGA 4 cut(s) 222, 268, 961, 1323
TasI AATT 7 cut(s) 314, 755, 885, 906, 972, 1135, 1231
TauI GCSGC 4 cut(s) 51, 54, 111, 181
TfiI GAWTC 4 cut(s) 958, 1043, 1083, 1291
Tru1I TTAA 5 cut(s) 249, 297, 758, 971, 1095
Tru9I TTAA 5 cut(s) 249, 297, 758, 971, 1095
TseFI GTSAC 2 cut(s) 343, 871
Tsp45I GTSAC 2 cut(s) 343, 871
TspDTI ATGAA 2 cut(s) 596, 767
TspGWI ACGGA 2 cut(s) 433, 540
TspMI CCCGGG 1 cut(s) 1078
VneI GTGCAC 1 cut(s) 501
XapI RAATTY 2 cut(s) 314, 885
XceI RCATGY 2 cut(s) 968, 1322
XmaI CCCGGG 1 cut(s) 1078
XmiI GTMKAC 2 cut(s) 1312, 1323
XspI CTAG 1 cut(s) 371
ZraI GACGTC 1 cut(s) 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.