Rh2CG327000

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000942
Physical Location & Seq
Reverse (-)
42468839 .. 42471133
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG327000.1

Sequence Viewer

Length: 969 bp
ATGGAGGAGGAGTTTGTGACGAACCAGGAGCGGCTGAAGCCTTATGAGGAGAAGGCTGAGGAGGACAGATCTAAGGTCGACGATCTACGTGCCTCGCCCATGAGCGTCGGGAATCTCGAGGAGCTGATCGACGAGAACCACGCGATTGTTTCGTCGTCGATGGGGCCCGAGTACTATGTGGGGGTCCTCTCCTTTGTCGATAAGGACCAGTTGGAGCCTGGGTGCGCCATTTTGATGCACAATAAGAAAGATGGGAAAGCTATTGTTGGGGAGGCCACCATTGAGGCAAACACGGTGTATGGTGCGTTGCGGGGGTTAGAGACTTTCAGCCAATTGTGTACTTTTGACTATGAAACTAAATCCATGCAAGTACATAAGTCACCATGGAACATACGAGACAAACCAAGGTTTGCATATCGAGGGCTTTTGCTTGGTGCAACTATAGTTCATGGAGTTCAAAACTTGTGGGTTTCACAACCCCCTCCAAATATTCAATATGCAGCTATAGTGATTGGTGGTTCGTTTATTATTGAAGGCGCTTCTCTTCTTGTTCCCATACAAGCTGTCAAGAAAGGTGCTGCTGCTGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATGATCCTACCTCTGTTGCAGTCATGACTAAGGATGGCGCTGCTGTAGCTGCTCTTGCTATTGCTGCAGCATCATTGGTTGCTGTAAATTTCACAGGAAATGGAATTTACGATCCTATAGGTTCAATTGTAGTTGGCAACCTTCTTGGAATGGTGGCTATCTTTCTGATACAAAGGAATCGACATGCTTTAATTGGTAGGGCAATGGATGACTATGATGTAGAGAAGGTTCTTCAGTTCTTGAAAAATGACCTGGTTGTAGATTCTTTCTTTTTATTTGTTTTGCACGTGGTGCATTCTTGGTTTTCCTTTTGCTGTTGGCATATTAATTTACATTTTGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

322

Amino Acids

35.62

Weight (kDa)

5.47

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 36 - 82 6.9e-10 Proteasomal ATPase OB C-terminal domain
Glycohydro_20b2 PF14845 90 - 112 1.3e-06 beta-acetyl hexosaminidase like
Cation_efflux PF01545 148 - 271 1.9e-06 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 143
AciI CCGC 2 cut(s) 31, 310
AclWI GGATC 2 cut(s) 620, 728
AcsI RAATTY 2 cut(s) 709, 726
AcuI CTGAAG 2 cut(s) 56, 839
AcvI CACGTG 1 cut(s) 910
AdeI CACNNNGTG 1 cut(s) 913
AfaI GTAC 3 cut(s) 173, 340, 372
AgsI TTSAA 5 cut(s) 458, 494, 533, 747, 865
AjnI CCWGG 3 cut(s) 24, 217, 873
AluBI AGCT 5 cut(s) 124, 260, 503, 563, 671
AluI AGCT 5 cut(s) 124, 260, 503, 563, 671
Alw26I GTCTC 3 cut(s) 314, 390, 597
AlwI GGATC 2 cut(s) 620, 728
Ama87I CYCGRG 2 cut(s) 116, 167
AoxI GGCC 2 cut(s) 164, 273
ApaI GGGCCC 1 cut(s) 168
ApeKI GCWGC 7 cut(s) 500, 578, 581, 662, 671, 686, 689
ApoI RAATTY 2 cut(s) 709, 726
AseI ATTAAT 1 cut(s) 948
AspLEI GCGC 3 cut(s) 227, 539, 662
AspS9I GGNCC 4 cut(s) 164, 165, 184, 205
AsuHPI GGTGA 1 cut(s) 372
AvaI CYCGRG 2 cut(s) 116, 167
AvaII GGWCC 2 cut(s) 184, 205
BaeGI GKGCMC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 168
BbrPI CACGTG 1 cut(s) 910
BbvCI CCTCAGC 2 cut(s) 57, 585
BbvI GCAGC 7 cut(s) 512, 565, 568, 649, 658, 673, 701
BccI CCATC 3 cut(s) 154, 245, 650
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 3 cut(s) 26, 219, 875
BcoDI GTCTC 3 cut(s) 314, 390, 597
BfmI CTRYAG 5 cut(s) 441, 504, 666, 687, 738
BfoI RGCGCY 2 cut(s) 540, 663
BglII AGATCT 1 cut(s) 68
BisI GCNGC 8 cut(s) 32, 501, 579, 582, 663, 672, 687, 690
BlsI GCNGC 8 cut(s) 33, 502, 580, 583, 664, 673, 688, 691
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 3 cut(s) 26, 219, 875
Bme18I GGWCC 2 cut(s) 184, 205
BmeT110I CYCGRG 2 cut(s) 116, 167
BmgT120I GGNCC 4 cut(s) 164, 165, 184, 205
BmiI GGNNCC 4 cut(s) 165, 166, 185, 216
BmrFI CCNGG 3 cut(s) 26, 219, 875
BmsI GCATC 2 cut(s) 225, 701
Bpu10I CCTNAGC 2 cut(s) 57, 585
BsaAI YACGTR 2 cut(s) 89, 910
BsaJI CCNNGG 3 cut(s) 218, 383, 404
BsaXI ACNNNNNCTCC 3 cut(s) 26, 206, 236
Bse1I ACTGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 831
BseBI CCWGG 3 cut(s) 26, 219, 875
BseDI CCNNGG 3 cut(s) 218, 383, 404
BseGI GGATG 2 cut(s) 661, 835
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 2 cut(s) 48, 576
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 5 cut(s) 20, 23, 62, 74, 134
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 7 cut(s) 512, 565, 568, 649, 658, 673, 701
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 166, 275
BsiHKCI CYCGRG 2 cut(s) 116, 167
BsmAI GTCTC 3 cut(s) 314, 390, 597
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 2 cut(s) 166, 275
BsoBI CYCGRG 2 cut(s) 116, 167
Bsp120I GGGCCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 5 cut(s) 68, 82, 126, 625, 733
Bsp19I CCATGG 1 cut(s) 383
BspACI CCGC 2 cut(s) 31, 310
BspANI GGCC 2 cut(s) 166, 275
BspCNI CTCAG 2 cut(s) 49, 577
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 2 cut(s) 622, 645
BspLI GGNNCC 4 cut(s) 165, 166, 185, 216
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 2 cut(s) 620, 728
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 1 cut(s) 208
BssECI CCNNGG 3 cut(s) 218, 383, 404
BssMI GATC 5 cut(s) 68, 82, 126, 625, 733
BssT1I CCWWGG 2 cut(s) 383, 404
Bst2UI CCWGG 3 cut(s) 26, 219, 875
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 549
BstAPI GCANNNNNTGC 1 cut(s) 913
BstBAI YACGTR 2 cut(s) 89, 910
BstDEI CTNAG 4 cut(s) 57, 72, 585, 651
BstDSI CCRYGG 1 cut(s) 383
BstF5I GGATG 2 cut(s) 661, 835
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 2 cut(s) 540, 663
BstHHI GCGC 3 cut(s) 227, 539, 662
BstKTI GATC 5 cut(s) 71, 85, 129, 628, 736
BstMAI GTCTC 3 cut(s) 314, 390, 597
BstMBI GATC 5 cut(s) 68, 82, 126, 625, 733
BstMWI GCNNNNNNNGC 6 cut(s) 37, 668, 671, 677, 686, 913
BstNI CCWGG 3 cut(s) 26, 219, 875
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 3 cut(s) 24, 217, 873
BstSFI CTRYAG 5 cut(s) 441, 504, 666, 687, 738
BstSLI GKGCMC 1 cut(s) 168
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 7 cut(s) 512, 565, 568, 649, 658, 673, 701
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 2 cut(s) 166, 275
BtgI CCRYGG 1 cut(s) 383
BtsCI GGATG 2 cut(s) 661, 835
CciI TCATGA 2 cut(s) 622, 645
CfoI GCGC 3 cut(s) 227, 539, 662
Cfr13I GGNCC 4 cut(s) 164, 165, 184, 205
CseI GACGC 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 3 cut(s) 172, 339, 371
CspCI CAANNNNNGTGG 2 cut(s) 446, 481
CviAII CATG 8 cut(s) 100, 364, 384, 449, 592, 623, 646, 806
CviQI GTAC 3 cut(s) 172, 339, 371
DdeI CTNAG 4 cut(s) 57, 72, 585, 651
DpnI GATC 5 cut(s) 70, 84, 128, 627, 735
DpnII GATC 5 cut(s) 68, 82, 126, 625, 733
DraIII CACNNNGTG 1 cut(s) 913
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Eco130I CCWWGG 2 cut(s) 383, 404
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 2 cut(s) 184, 205
Eco57I CTGAAG 2 cut(s) 56, 839
Eco72I CACGTG 1 cut(s) 910
Eco88I CYCGRG 2 cut(s) 116, 167
EcoO109I RGGNCCY 2 cut(s) 164, 184
EcoRII CCWGG 3 cut(s) 24, 217, 873
EcoT14I CCWWGG 2 cut(s) 383, 404
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 2 cut(s) 383, 404
FaeI CATG 8 cut(s) 103, 367, 387, 452, 595, 626, 649, 809
FatI CATG 8 cut(s) 99, 363, 383, 448, 591, 622, 645, 805
FauI CCCGC 1 cut(s) 303
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 8 cut(s) 32, 501, 579, 582, 663, 672, 687, 690
FokI GGATG 2 cut(s) 668, 842
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 8 cut(s) 32, 501, 579, 582, 663, 672, 687, 690
GlaI GCGC 3 cut(s) 226, 538, 661
GluI GCNGC 8 cut(s) 32, 501, 579, 582, 663, 672, 687, 690
HaeII RGCGCY 2 cut(s) 540, 663
HaeIII GGCC 2 cut(s) 166, 275
HgaI GACGC 1 cut(s) 94
HhaI GCGC 3 cut(s) 227, 539, 662
Hin1II CATG 8 cut(s) 103, 367, 387, 452, 595, 626, 649, 809
Hin6I GCGC 3 cut(s) 225, 537, 660
HinP1I GCGC 3 cut(s) 225, 537, 660
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 3 cut(s) 112, 799, 884
HphI GGTGA 1 cut(s) 372
Hpy166II GTNNAC 2 cut(s) 79, 339
Hpy188I TCNGA 1 cut(s) 789
Hpy188III TCNNGA 6 cut(s) 109, 116, 568, 623, 646, 862
Hpy8I GTNNAC 2 cut(s) 79, 339
Hpy99I CGWCG 5 cut(s) 83, 110, 134, 157, 160
HpyAV CCTTC 4 cut(s) 46, 527, 773, 841
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 88, 909
HpyCH4V TGCA 9 cut(s) 238, 367, 413, 437, 500, 641, 689, 907, 916
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 668, 671, 677, 686, 913
HpyF3I CTNAG 4 cut(s) 57, 72, 585, 651
HpySE526I ACGT 2 cut(s) 88, 909
Hsp92II CATG 8 cut(s) 103, 367, 387, 452, 595, 626, 649, 809
HspAI GCGC 3 cut(s) 225, 537, 660
Kzo9I GATC 5 cut(s) 68, 82, 126, 625, 733
LmnI GCTCC 3 cut(s) 28, 121, 214
LpnPI CCDG 8 cut(s) 11, 38, 204, 221, 231, 702, 860, 887
Lsp1109I GCAGC 7 cut(s) 512, 565, 568, 649, 658, 673, 701
LweI GCATC 2 cut(s) 225, 701
MabI ACCWGGT 1 cut(s) 873
MaeII ACGT 2 cut(s) 88, 909
MaeIII GTNAC 2 cut(s) 16, 378
MalI GATC 5 cut(s) 70, 84, 128, 627, 735
MbiI CCGCTC 1 cut(s) 31
MboI GATC 5 cut(s) 68, 82, 126, 625, 733
MboII GAAGA 2 cut(s) 536, 845
MfeI CAATTG 2 cut(s) 332, 747
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 7 cut(s) 332, 596, 709, 726, 747, 813, 949
MmeI TCCRAC 1 cut(s) 192
MseI TTAA 3 cut(s) 599, 812, 948
MslI CAYNNNNRTG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 26, 219, 875
MunI CAATTG 2 cut(s) 332, 747
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 3 cut(s) 26, 219, 875
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 6 cut(s) 37, 668, 671, 677, 686, 913
NcoI CCATGG 1 cut(s) 383
NdeII GATC 5 cut(s) 68, 82, 126, 625, 733
NlaIII CATG 8 cut(s) 103, 367, 387, 452, 595, 626, 649, 809
NlaIV GGNNCC 4 cut(s) 165, 166, 185, 216
NmuCI GTSAC 2 cut(s) 16, 378
NspI RCATGY 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 2 cut(s) 622, 645
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 916
PfeI GAWTC 3 cut(s) 112, 799, 884
PkrI GCNGC 8 cut(s) 33, 502, 580, 583, 664, 673, 688, 691
PmaCI CACGTG 1 cut(s) 910
PmlI CACGTG 1 cut(s) 910
Ppu21I YACGTR 2 cut(s) 89, 910
PpuMI RGGWCCY 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 3 cut(s) 24, 217, 873
PspCI CACGTG 1 cut(s) 910
PspGI CCWGG 3 cut(s) 24, 217, 873
PspN4I GGNNCC 4 cut(s) 165, 166, 185, 216
PspOMI GGGCCC 1 cut(s) 164
PspPI GGNCC 4 cut(s) 164, 165, 184, 205
PspPPI RGGWCCY 1 cut(s) 184
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 3 cut(s) 173, 340, 372
RsaNI GTAC 3 cut(s) 172, 339, 371
RseI CAYNNNNRTG 1 cut(s) 233
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 3 cut(s) 599, 812, 948
SatI GCNGC 8 cut(s) 32, 501, 579, 582, 663, 672, 687, 690
Sau3AI GATC 5 cut(s) 68, 82, 126, 625, 733
Sau96I GGNCC 4 cut(s) 164, 165, 184, 205
ScaI AGTACT 1 cut(s) 173
ScrFI CCNGG 3 cut(s) 26, 219, 875
SduI GDGCHC 1 cut(s) 168
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 2 cut(s) 225, 701
SfcI CTRYAG 5 cut(s) 441, 504, 666, 687, 738
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 2 cut(s) 184, 205
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 7 cut(s) 332, 596, 709, 726, 747, 813, 949
SsiI CCGC 2 cut(s) 31, 310
SspI AATATT 1 cut(s) 490
StyD4I CCNGG 3 cut(s) 24, 217, 873
StyI CCWWGG 2 cut(s) 383, 404
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 2 cut(s) 91, 912
TaqI TCGA 7 cut(s) 78, 117, 129, 158, 198, 418, 802
TasI AATT 7 cut(s) 332, 596, 709, 726, 747, 813, 949
TatI WGTACW 3 cut(s) 171, 338, 370
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 3 cut(s) 112, 799, 884
Tru1I TTAA 3 cut(s) 599, 812, 948
Tru9I TTAA 3 cut(s) 599, 812, 948
TseFI GTSAC 2 cut(s) 16, 378
TseI GCWGC 7 cut(s) 500, 578, 581, 662, 671, 686, 689
Tsp45I GTSAC 2 cut(s) 16, 378
TspDTI ATGAA 3 cut(s) 366, 437, 608
VpaK11BI GGWCC 2 cut(s) 184, 205
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 2 cut(s) 709, 726
XceI RCATGY 1 cut(s) 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.