MD09G1160600.v1.1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000942
Physical Location & Seq
Reverse (-)
12954610 .. 12958587
3978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1160600.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGAGAACGTCCTCCTACACTCTCGTCTCCCGCCTTCGCCCCACCCACCGCTTCAGTCCTCGCTGCTCCTCCCAATTTCTGCTCTTTCACTACTCAGACCCCAAGGACGACGATCGCAGCGTCCCCCAAAACGCTCCGTTTAGCTCCTCGGCTCACACTCTGCTGCGCGGCCTGGTTTCCTCGGCTGCTTGCTCGAAACGGGTGGTCTTGCCGGACTTGGTTTCTTCGGGTAGTGTTGCTCTTGGTCAACAGCAGCAGCAGCACTTGCTTCATCGCAACTTCTTTACTAGAGCTAAGCAGGTGAAGATTGAATTCAATGACCAGCATAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTTGTATTTTCTCTCAAGTTTGGTGTCTGGTTATCGACCTCTAGCCATGTTATGTTTGCTGAAGTTGTGCACTCGGTTGCGGATTTTGCAAATCAGGCGCTTCTTGCTTATGGTTTGAGTAGCTCAAGGCGCGCACCGGATGCTCTCCACCCTTATGGATACTCCAAGGAAAGATTTGTCTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCGGGTGCGACTATCGTTCATGGAGTTCAAAACTTGTGGACTTCACAGCCTCCTCCAAATAGTCAGTATGCAGCTCTTGTGATTGGTGGCTCATTTATTATTGAAGGTGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGGTGCAGCTGCTGAGGGCATGAAATTGAGAGACTTTATATGGCGCGGACACGATCCCACATCTGTTGCAGTCATGACAGAGGATGGCGCAGCTGTAACTGGTCTTGCTATTGCTGCGGCATCACTGGTTGCAGTGAATAGAACAGGAAATGCAATTTATGACCCCATAGGATCGATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATTTTTCTGATCCAAAGGAATCGACATGCTTTGATTGGCAGGGCAATGGACGACCATGATATGGAGAAGGTTCTCCAGTTTTTGAAAAATGACCCGGTTGTGGATTCTCTATATGATTGCAAAAGTGAGGTGATTGGTCCTGGATTCTTCAGATTTAAGGCTGAAATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTATCTCTCTAGGACTGGGCGTGAAGAATGGGCGAGACAGTTTTGTGAAGCTGCAAAGGAGAAGGATGATACTGCATTGCTTAAAATGATGTCAAACTATGGTGAAGAGGTAGTGACAGCGTTAGGAAGTGAAGTTGATAGATTGGAAAAGGAGATCCAAGCACTTGTTCCTGGTATACGGCATGTTGATATCGAGGCACACAATCCAATAGAACTATCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

453

Amino Acids

49.61

Weight (kDa)

7.26

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 115 - 323 1.3e-29 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 289
Acc36I ACCTGC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 997
AccI GTMKAC 1 cut(s) 1309
AccII CGCG 3 cut(s) 168, 498, 774
AciI CCGC 6 cut(s) 31, 49, 168, 446, 774, 845
AclWI GGATC 4 cut(s) 776, 907, 940, 1282
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 3 cut(s) 37, 447, 1069
AfiI CCNNNNNNNGG 2 cut(s) 997, 1036
AgsI TTSAA 7 cut(s) 311, 316, 614, 689, 1021, 1111, 1130
AjnI CCWGG 3 cut(s) 171, 1075, 1303
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
AluBI AGCT 8 cut(s) 144, 293, 489, 659, 719, 737, 821, 1184
AluI AGCT 8 cut(s) 144, 293, 489, 659, 719, 737, 821, 1184
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 3 cut(s) 31, 753, 1162
Alw44I GTGCAC 1 cut(s) 434
AlwI GGATC 4 cut(s) 776, 907, 940, 1282
AlwNI CAGNNNCTG 1 cut(s) 740
AoxI GGCC 1 cut(s) 169
ApaLI GTGCAC 1 cut(s) 434
ApoI RAATTY 1 cut(s) 311
AspLEI GCGC 6 cut(s) 168, 466, 498, 500, 774, 818
AspS9I GGNCC 1 cut(s) 1073
AsuC2I CCSGG 1 cut(s) 1031
AsuHPI GGTGA 3 cut(s) 313, 1078, 1247
AvaII GGWCC 1 cut(s) 1073
BaeGI GKGCMC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 741
BccI CCATC 1 cut(s) 806
BceAI ACGGC 1 cut(s) 1328
BciT130I CCWGG 3 cut(s) 173, 1077, 1305
BciVI GTATCC 1 cut(s) 518
BcnI CCSGG 1 cut(s) 1031
BcoDI GTCTC 3 cut(s) 31, 753, 1162
BfaI CTAG 3 cut(s) 288, 408, 1143
BfoI RGCGCY 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 289
BfuI GTATCC 1 cut(s) 518
BlpI GCTNAGC 1 cut(s) 294
Bme1390I CCNGG 4 cut(s) 173, 1031, 1077, 1305
Bme18I GGWCC 1 cut(s) 1073
BmgT120I GGNCC 1 cut(s) 1073
BmrFI CCNGG 4 cut(s) 173, 1031, 1077, 1305
BmrI ACTGGG 1 cut(s) 1158
BmsI GCATC 2 cut(s) 496, 857
BmuI ACTGGG 1 cut(s) 1158
BpmI CTGGAG 1 cut(s) 995
Bpu10I CCTNAGC 1 cut(s) 741
Bpu1102I GCTNAGC 1 cut(s) 294
BpuEI CTTGAG 2 cut(s) 365, 475
BpuMI CCSGG 1 cut(s) 1031
Bsa29I ATCGAT 1 cut(s) 902
BsaJI CCNNGG 4 cut(s) 102, 147, 180, 531
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 1107, 1137
Bsc4I CCNNNNNNNGG 2 cut(s) 997, 1036
Bse1I ACTGG 4 cut(s) 832, 858, 1012, 1153
Bse3DI GCAATG 2 cut(s) 987, 1208
BseBI CCWGG 3 cut(s) 173, 1077, 1305
BseCI ATCGAT 1 cut(s) 902
BseDI CCNNGG 4 cut(s) 102, 147, 180, 531
BseGI GGATG 3 cut(s) 511, 817, 1204
BseLI CCNNNNNNNGG 2 cut(s) 997, 1036
BseMI GCAATG 2 cut(s) 987, 1208
BseMII CTCAG 2 cut(s) 108, 732
BseNI ACTGG 4 cut(s) 832, 858, 1012, 1153
BsePI GCGCGC 1 cut(s) 496
BseRI GAGGAG 3 cut(s) 58, 136, 627
BseSI GKGCMC 1 cut(s) 438
BsgI GTGCAG 1 cut(s) 753
Bsh1236I CGCG 3 cut(s) 168, 498, 774
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 902
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 438
BsiSI CCGG 3 cut(s) 212, 503, 1031
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 997, 1036
BsmAI GTCTC 3 cut(s) 31, 753, 1162
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 5 cut(s) 112, 781, 899, 945, 1287
Bsp1720I GCTNAGC 1 cut(s) 294
BspACI CCGC 6 cut(s) 31, 49, 168, 446, 774, 845
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 107, 733
BspDI ATCGAT 1 cut(s) 902
BspFNI CGCG 3 cut(s) 168, 498, 774
BspHI TCATGA 1 cut(s) 801
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 4 cut(s) 776, 907, 940, 1282
BsrDI GCAATG 2 cut(s) 987, 1208
BsrI ACTGG 4 cut(s) 832, 858, 1012, 1153
BssECI CCNNGG 4 cut(s) 102, 147, 180, 531
BssHII GCGCGC 1 cut(s) 496
BssMI GATC 5 cut(s) 112, 781, 899, 945, 1287
BssNAI GTATAC 1 cut(s) 1310
BssT1I CCWWGG 2 cut(s) 102, 531
Bst1107I GTATAC 1 cut(s) 1310
Bst2UI CCWGG 3 cut(s) 173, 1077, 1305
Bst4CI ACNGT 1 cut(s) 1173
Bst6I CTCTTC 2 cut(s) 705, 1233
BstAPI GCANNNNNTGC 2 cut(s) 265, 506
BstC8I GCNNGC 2 cut(s) 190, 498
BstDEI CTNAG 3 cut(s) 94, 294, 741
BstF5I GGATG 3 cut(s) 511, 817, 1204
BstFNI CGCG 3 cut(s) 168, 498, 774
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 6 cut(s) 168, 466, 498, 500, 774, 818
BstKTI GATC 5 cut(s) 115, 784, 902, 948, 1290
BstMAI GTCTC 3 cut(s) 31, 753, 1162
BstMBI GATC 5 cut(s) 112, 781, 899, 945, 1287
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 9 cut(s) 259, 265, 452, 461, 470, 495, 506, 716, 842
BstNI CCWGG 3 cut(s) 173, 1077, 1305
BstNSI RCATGY 2 cut(s) 965, 1319
BstSCI CCNGG 4 cut(s) 171, 1029, 1075, 1303
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 3 cut(s) 168, 498, 774
BstX2I RGATCY 1 cut(s) 1287
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 1 cut(s) 1287
BstZ17I GTATAC 1 cut(s) 1310
Bsu15I ATCGAT 1 cut(s) 902
BsuI GTATCC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 902
BtgZI GCGATG 1 cut(s) 257
BtsCI GGATG 3 cut(s) 511, 817, 1204
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 2 cut(s) 851, 867
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 190, 498
CaiI CAGNNNCTG 1 cut(s) 740
CciI TCATGA 1 cut(s) 801
CfoI GCGC 6 cut(s) 168, 466, 498, 500, 774, 818
Cfr13I GGNCC 1 cut(s) 1073
ClaI ATCGAT 1 cut(s) 902
CseI GACGC 1 cut(s) 109
CspCI CAANNNNNGTGG 4 cut(s) 602, 637, 775, 810
CviAII CATG 8 cut(s) 413, 605, 748, 802, 962, 992, 1316, 1356
DdeI CTNAG 3 cut(s) 94, 294, 741
DpnI GATC 5 cut(s) 114, 783, 901, 947, 1289
DpnII GATC 5 cut(s) 112, 781, 899, 945, 1287
Eam1104I CTCTTC 2 cut(s) 705, 1233
EarI CTCTTC 2 cut(s) 705, 1233
Eco130I CCWWGG 2 cut(s) 102, 531
Eco32I GATATC 2 cut(s) 558, 1324
Eco47I GGWCC 1 cut(s) 1073
Eco57I CTGAAG 3 cut(s) 37, 447, 1069
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 3 cut(s) 171, 1075, 1303
EcoRV GATATC 2 cut(s) 558, 1324
EcoT14I CCWWGG 2 cut(s) 102, 531
ErhI CCWWGG 2 cut(s) 102, 531
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 8 cut(s) 416, 608, 751, 805, 965, 995, 1319, 1359
FaqI GGGAC 1 cut(s) 107
FatI CATG 8 cut(s) 412, 604, 747, 801, 961, 991, 1315, 1355
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1309
FokI GGATG 3 cut(s) 518, 824, 1211
FspBI CTAG 3 cut(s) 288, 408, 1143
GlaI GCGC 6 cut(s) 167, 465, 497, 499, 773, 817
GsuI CTGGAG 1 cut(s) 995
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 1 cut(s) 171
HapII CCGG 3 cut(s) 212, 503, 1031
HgaI GACGC 1 cut(s) 109
HhaI GCGC 6 cut(s) 168, 466, 498, 500, 774, 818
Hin1II CATG 8 cut(s) 416, 608, 751, 805, 965, 995, 1319, 1359
Hin6I GCGC 6 cut(s) 166, 464, 496, 498, 772, 816
HinP1I GCGC 6 cut(s) 166, 464, 496, 498, 772, 816
HincII GTYRAC 1 cut(s) 248
HindII GTYRAC 1 cut(s) 248
HinfI GANTC 3 cut(s) 955, 1040, 1080
HpaII CCGG 3 cut(s) 212, 503, 1031
HphI GGTGA 3 cut(s) 313, 1078, 1247
Hpy166II GTNNAC 4 cut(s) 248, 436, 624, 1310
Hpy188I TCNGA 3 cut(s) 97, 945, 1088
Hpy188III TCNNGA 2 cut(s) 724, 802
Hpy8I GTNNAC 4 cut(s) 248, 436, 624, 1310
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 44, 683, 997, 1189
HpyCH4III ACNGT 1 cut(s) 1173
HpyCH4IV ACGT 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 9 cut(s) 259, 265, 452, 461, 470, 495, 506, 716, 842
HpyF3I CTNAG 3 cut(s) 94, 294, 741
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 8 cut(s) 416, 608, 751, 805, 965, 995, 1319, 1359
HspAI GCGC 6 cut(s) 166, 464, 496, 498, 772, 816
Kzo9I GATC 5 cut(s) 112, 781, 899, 945, 1287
LmnI GCTCC 3 cut(s) 71, 139, 149
LweI GCATC 2 cut(s) 496, 857
MaeI CTAG 3 cut(s) 288, 408, 1143
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 3 cut(s) 340, 823, 1246
MalI GATC 5 cut(s) 114, 783, 901, 947, 1289
MboI GATC 5 cut(s) 112, 781, 899, 945, 1287
MboII GAAGA 6 cut(s) 216, 316, 692, 1075, 1169, 1250
MflI RGATCY 1 cut(s) 1287
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 5 cut(s) 74, 311, 752, 882, 1132
MseI TTAA 2 cut(s) 1092, 1215
MslI CAYNNNNRTG 2 cut(s) 355, 519
MspA1I CMGCKG 2 cut(s) 737, 821
MspI CCGG 3 cut(s) 212, 503, 1031
MspR9I CCNGG 4 cut(s) 173, 1031, 1077, 1305
MvaI CCWGG 3 cut(s) 173, 1077, 1305
MvnI CGCG 3 cut(s) 168, 498, 774
MwoI GCNNNNNNNGC 9 cut(s) 259, 265, 452, 461, 470, 495, 506, 716, 842
NciI CCSGG 1 cut(s) 1031
NdeII GATC 5 cut(s) 112, 781, 899, 945, 1287
NlaIII CATG 8 cut(s) 416, 608, 751, 805, 965, 995, 1319, 1359
NmeAIII GCCGAG 2 cut(s) 128, 161
NmuCI GTSAC 2 cut(s) 340, 1246
NspI RCATGY 2 cut(s) 965, 1319
PagI TCATGA 1 cut(s) 801
PaqCI CACCTGC 1 cut(s) 289
PauI GCGCGC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 3 cut(s) 955, 1040, 1080
PflMI CCANNNNNTGG 1 cut(s) 997
PfoI TCCNGGA 1 cut(s) 1075
Ple19I CGATCG 1 cut(s) 115
Psp6I CCWGG 3 cut(s) 171, 1075, 1303
PspGI CCWGG 3 cut(s) 171, 1075, 1303
PspPI GGNCC 1 cut(s) 1073
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 1 cut(s) 1287
PteI GCGCGC 1 cut(s) 496
PvuI CGATCG 1 cut(s) 115
PvuII CAGCTG 2 cut(s) 737, 821
RseI CAYNNNNRTG 2 cut(s) 355, 519
SaqAI TTAA 2 cut(s) 1092, 1215
Sau3AI GATC 5 cut(s) 112, 781, 899, 945, 1287
Sau96I GGNCC 1 cut(s) 1073
ScrFI CCNGG 4 cut(s) 173, 1031, 1077, 1305
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 2 cut(s) 496, 857
SinI GGWCC 1 cut(s) 1073
SmiMI CAYNNNNRTG 2 cut(s) 355, 519
SmlI CTYRAG 2 cut(s) 380, 490
SmoI CTYRAG 2 cut(s) 380, 490
Sse9I AATT 5 cut(s) 74, 311, 752, 882, 1132
SsiI CCGC 6 cut(s) 31, 49, 168, 446, 774, 845
SspMI CTAG 3 cut(s) 288, 408, 1143
StyD4I CCNGG 4 cut(s) 171, 1029, 1075, 1303
StyI CCWWGG 2 cut(s) 102, 531
TaaI ACNGT 1 cut(s) 1173
TaiI ACGT 1 cut(s) 11
TaqI TCGA 5 cut(s) 194, 401, 902, 958, 1326
TasI AATT 5 cut(s) 74, 311, 752, 882, 1132
TauI GCSGC 2 cut(s) 171, 848
TfiI GAWTC 3 cut(s) 955, 1040, 1080
Tru1I TTAA 2 cut(s) 1092, 1215
Tru9I TTAA 2 cut(s) 1092, 1215
TscAI CASTG 2 cut(s) 858, 867
TseFI GTSAC 2 cut(s) 340, 1246
Tsp45I GTSAC 2 cut(s) 340, 1246
TspDTI ATGAA 3 cut(s) 260, 593, 764
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 2 cut(s) 858, 867
Van91I CCANNNNNTGG 1 cut(s) 997
VneI GTGCAC 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 1073
XapI RAATTY 1 cut(s) 311
XceI RCATGY 2 cut(s) 965, 1319
XmiI GTMKAC 1 cut(s) 1309
XspI CTAG 3 cut(s) 288, 408, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.