Rorug07G0127900

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000942
Physical Location & Seq
Forward (+)
10080783 .. 10082790
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0127900.1

Sequence Viewer

Length: 672 bp
ATGACGGGTCTCATAAACCCCGCTCCTCTACTTCACCTTCACCTTCAGAAGAAAGTGACAATGGAAGCTGGGGAGAAATCAGAAGAAACTGTATCAAAACATTCTCCGAAACGAGCCGGAACTGTCAACTGGGGAACAGCAACTGTCGCCGGAGTATTTGCAGGCATGTTGTACGGGGGTAGCAAGGAGGCATCTGCTTCGGTTAGCAAGGATGCTGAAGTAATGTTGAAGCTTGGGAGCACACCAGACAAGCGTGAGCAATATCGTTTGATGAGAGATGCAATGGAGAAACGGTTCATTCGAGTCACGCGGGGCTCAATAGTTGGTGGAGTGCGTCTTGGATTGTTCACTGCTACCTTTTATGGTTTACAAAATTTGCTTGCCGAGAAAAGGGGTGTGCAAGATGTTTTCAATATTGTGGGAGCTGGTTCAGCCACAGCTGCGACATTCGGGCTATTATTGCCAGGATCATTACAATGGCGTGCAAGAAATATGTTACTGGGTTCGATCCTTGGAGCAGTCATTGGTTTCCCAATTGGGTGGGCACACTTGAAGCTGGTGCAGATAGCAAATGAAGGGAATCCTGCTGCGCATCCTAATTCAGATCAAAGAGAAGCAAAGAGTGGTGTTGGTGCTGCCATTGAGAGGCTTGAAGAAAATTTGAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

223

Amino Acids

23.85

Weight (kDa)

9.84

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 45 - 175 4.5e-09 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 591
AccBSI CCGCTC 1 cut(s) 23
AccII CGCG 1 cut(s) 310
AciI CCGC 2 cut(s) 21, 310
AclWI GGATC 2 cut(s) 475, 502
AcsI RAATTY 2 cut(s) 373, 658
AcuI CTGAAG 2 cut(s) 29, 237
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 390, 645
AgsI TTSAA 5 cut(s) 229, 412, 553, 653, 664
AjnI CCWGG 1 cut(s) 463
AloI GAACNNNNNNTCC 2 cut(s) 278, 310
AluBI AGCT 5 cut(s) 68, 232, 425, 440, 556
AluI AGCT 5 cut(s) 68, 232, 425, 440, 556
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 2 cut(s) 475, 502
AlwNI CAGNNNCTG 1 cut(s) 143
ApeKI GCWGC 3 cut(s) 440, 587, 635
ApoI RAATTY 2 cut(s) 373, 658
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 1 cut(s) 592
AsuHPI GGTGA 2 cut(s) 26, 32
BaeGI GKGCMC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 3 cut(s) 427, 574, 622
BcgI CGANNNNNNTGC 2 cut(s) 180, 214
BciT130I CCWGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 14
BisI GCNGC 3 cut(s) 441, 588, 636
BlsI GCNGC 3 cut(s) 442, 589, 637
Bme1390I CCNGG 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 465
BmrI ACTGGG 2 cut(s) 139, 509
BmsI GCATC 4 cut(s) 200, 202, 268, 601
BmuI ACTGGG 2 cut(s) 139, 509
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 645
Bse1I ACTGG 2 cut(s) 134, 504
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 2 cut(s) 217, 592
BseLI CCNNNNNNNGG 2 cut(s) 390, 645
BseMI GCAATG 1 cut(s) 288
BseNI ACTGG 2 cut(s) 134, 504
BseRI GAGGAG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 3 cut(s) 427, 574, 622
BseYI CCCAGC 1 cut(s) 68
BsgI GTGCAG 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 2 cut(s) 117, 150
BslI CCNNNNNNNGG 2 cut(s) 390, 645
BsmAI GTCTC 1 cut(s) 14
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 3 cut(s) 242, 317, 547
Bsp143I GATC 3 cut(s) 467, 507, 604
BspACI CCGC 2 cut(s) 21, 310
BspFNI CGCG 1 cut(s) 310
BspPI GGATC 2 cut(s) 475, 502
BspTNI GGTCTC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 2 cut(s) 134, 504
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 3 cut(s) 467, 507, 604
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 4 cut(s) 91, 124, 145, 294
BstC8I GCNNGC 3 cut(s) 163, 381, 483
BstF5I GGATG 2 cut(s) 217, 592
BstFNI CGCG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 3 cut(s) 470, 510, 607
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 3 cut(s) 467, 507, 604
BstMWI GCNNNNNNNGC 4 cut(s) 146, 431, 440, 460
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 463
BstSLI GKGCMC 1 cut(s) 547
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 3 cut(s) 427, 574, 622
BstXI CCANNNNNNTGG 1 cut(s) 540
BtsCI GGATG 2 cut(s) 217, 592
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 163, 381, 483
CaiI CAGNNNCTG 1 cut(s) 143
CfoI GCGC 1 cut(s) 592
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 172
CviAII CATG 1 cut(s) 166
CviQI GTAC 1 cut(s) 172
DpnI GATC 3 cut(s) 469, 509, 606
DpnII GATC 3 cut(s) 467, 507, 604
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 14
Eco57I CTGAAG 2 cut(s) 29, 237
EcoRII CCWGG 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 1 cut(s) 169
FaiI YATR 4 cut(s) 14, 167, 363, 494
FatI CATG 1 cut(s) 165
FauI CCCGC 2 cut(s) 28, 303
Fnu4HI GCNGC 3 cut(s) 441, 588, 636
FokI GGATG 2 cut(s) 224, 579
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 3 cut(s) 441, 588, 636
FspI TGCGCA 1 cut(s) 591
GlaI GCGC 1 cut(s) 591
GluI GCNGC 3 cut(s) 441, 588, 636
GsaI CCCAGC 1 cut(s) 72
HapII CCGG 2 cut(s) 117, 150
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 592
Hin1II CATG 1 cut(s) 169
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 303, 580
HpaII CCGG 2 cut(s) 117, 150
HphI GGTGA 2 cut(s) 26, 32
Hpy166II GTNNAC 3 cut(s) 127, 348, 368
Hpy188I TCNGA 4 cut(s) 48, 82, 108, 604
Hpy8I GTNNAC 3 cut(s) 127, 348, 368
HpyAV CCTTC 3 cut(s) 47, 53, 569
HpyCH4III ACNGT 4 cut(s) 91, 124, 145, 294
HpyCH4V TGCA 5 cut(s) 161, 281, 400, 485, 562
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 431, 440, 460
Hsp92II CATG 1 cut(s) 169
HspAI GCGC 1 cut(s) 590
Kzo9I GATC 3 cut(s) 467, 507, 604
LmnI GCTCC 4 cut(s) 28, 237, 422, 515
Lsp1109I GCAGC 3 cut(s) 427, 574, 622
LweI GCATC 4 cut(s) 200, 202, 268, 601
MaeIII GTNAC 3 cut(s) 55, 304, 495
MalI GATC 3 cut(s) 469, 509, 606
MbiI CCGCTC 1 cut(s) 23
MboI GATC 3 cut(s) 467, 507, 604
MboII GAAGA 3 cut(s) 61, 95, 665
MfeI CAATTG 1 cut(s) 534
MhlI GDGCHC 3 cut(s) 242, 317, 547
MluCI AATT 4 cut(s) 373, 534, 598, 658
MlyI GAGTC 1 cut(s) 312
MnlI CCTC 3 cut(s) 36, 181, 639
MroXI GAANNNNTTC 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 475
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 2 cut(s) 117, 150
MspR9I CCNGG 1 cut(s) 465
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 4 cut(s) 146, 431, 440, 460
NdeII GATC 3 cut(s) 467, 507, 604
NlaIII CATG 1 cut(s) 169
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 2 cut(s) 55, 304
NsbI TGCGCA 1 cut(s) 591
NspI RCATGY 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 1 cut(s) 580
PkrI GCNGC 3 cut(s) 442, 589, 637
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 475
SatI GCNGC 3 cut(s) 441, 588, 636
Sau3AI GATC 3 cut(s) 467, 507, 604
SchI GAGTC 1 cut(s) 312
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 3 cut(s) 242, 317, 547
SetI ASST 8 cut(s) 39, 45, 70, 234, 359, 427, 442, 558
SfaNI GCATC 4 cut(s) 200, 202, 268, 601
SmiMI CAYNNNNRTG 1 cut(s) 475
Sse9I AATT 4 cut(s) 373, 534, 598, 658
SsiI CCGC 2 cut(s) 21, 310
SspI AATATT 1 cut(s) 415
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 4 cut(s) 91, 124, 145, 294
TaqI TCGA 2 cut(s) 301, 506
TasI AATT 4 cut(s) 373, 534, 598, 658
TfiI GAWTC 1 cut(s) 580
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 2 cut(s) 55, 304
TseI GCWGC 3 cut(s) 440, 587, 635
Tsp45I GTSAC 2 cut(s) 55, 304
TspDTI ATGAA 2 cut(s) 286, 588
TspRI CASTG 1 cut(s) 355
XapI RAATTY 2 cut(s) 373, 658
XceI RCATGY 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.