Prupe.3G019200_v2.0.a1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000942
Physical Location & Seq
Reverse (-)
1369203 .. 1374435
5233 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G019200.2

Sequence Viewer

Length: 1380 bp
ATGAGATCGTCCTCTTATACTCTGCTCTCTCGCCTCCGCCATACCCATCGCTTCAATTCGCGCTGCCAATCCCAATTTCTGCTCTTTCACTACTCAGACCCAAAGCTCACACAAAATAACGACTGCAGCTTCTCCCAGAACGCTCCGTTTAGCTCCTCCGCTCACACTCTGCTGCGCGGTCTGGTTTCGTCATCTGCTTGCTCAAAACGGCTCGTTCTGCTCGACTTGGTTTCTTCTGGTAGTGTTGTTCACCATCAACATCAGCAGCAGCACTTGTTCCATCGCAATTTCTTTACTAGAGCTAAACAGGTCAAGATTCAATTCAATGACGAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTTGTGTTTTCTCTCAAATTTGGTGTCTGGTTATCAACCGCCAGTCATGTTATGTTTGCTGAAGTTGTTCACTCAGTTGCAGATTTTGCAAATCAGGCGCTTCTTGCTTATGGTTTGAGTAGCTCAAGGCGTGCACCGGATGCTCTCCACCCTTATGGATACTCCAAGGAAAGATTTGTCTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCTGGTGCGACTATTGTTCATGGATTTCAAAACTTGTGGACTTCACAGCCCCCTCCAAATATTCAGTATGCAGCTCTTGTGATTGGTGGCTCATTTATTATTGAAGGTGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGGTGCAGCTGCTGAGGGCATGAAATTGAGAGACTACATTTGGCGTGGCCATGATCCCACATCTGTTGCTGTCATGACAGAGGATGGCGCGGCTGTAACTGGTCTTGCTATTGCTGCGGCATCATTGGTTGCAGTGAATAGAACAGGAAATGCAATTTATGATCCCATAGGATCAATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATTTTTCTGATCCAAAGGAATAGGCATGCTTTGATTGGTAGGGCAATGGACGACCATGATATGGAGAAGGTTCTCCAGTTCTTGAAAAATGACCCGGTTGTGGATTCCCTATATGATTGCAAAAGTGAGGTGATTGGTCCTGGGTTCTTCCGATTTAAGGCTGAAATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTATCTCCACAGGACTGGGCGTGAAGAATGGGCTAAGCAGTTTCGTGATGCTGCAAAGGAGAAGGATGATACTGCGTTGCTTAAAATCATGTCAAATTATGGTGAAGAGGTAGTGACAGCCTTAGGAAGTGAAGTTGATAGGTTGGAAAAGGAGATCCAAGAGCTTGTTCCCGGTATACGGCATGTCGACATTGAGATGTATCATGATAGCTCGAGGAAACTACTGCGTATTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

460

Amino Acids

51.08

Weight (kDa)

8.63

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1006
AccBSI CCGCTC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 1318, 1329
AccII CGCG 3 cut(s) 61, 177, 827
AciI CCGC 6 cut(s) 37, 159, 177, 414, 827, 854
AclWI GGATC 5 cut(s) 785, 893, 916, 949, 1291
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 456
AfiI CCNNNNNNNGG 4 cut(s) 1006, 1045, 1102, 1320
AgsI TTSAA 8 cut(s) 55, 320, 325, 623, 698, 1030, 1120, 1139
AjnI CCWGG 1 cut(s) 1084
AjuI GAANNNNNNNTTGG 4 cut(s) 60, 92, 1293, 1325
Alw21I GWGCWC 2 cut(s) 336, 511
Alw26I GTCTC 1 cut(s) 762
Alw44I GTGCAC 1 cut(s) 507
AlwI GGATC 5 cut(s) 785, 893, 916, 949, 1291
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 1354
AoxI GGCC 1 cut(s) 784
ApaLI GTGCAC 1 cut(s) 507
ApoI RAATTY 1 cut(s) 392
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 4 cut(s) 63, 177, 475, 827
AspS9I GGNCC 1 cut(s) 1082
AsuC2I CCSGG 2 cut(s) 1040, 1314
AsuHPI GGTGA 3 cut(s) 242, 1087, 1256
AvaI CYCGRG 1 cut(s) 1354
AvaII GGWCC 1 cut(s) 1082
AxyI CCTNAGG 1 cut(s) 1264
BaeGI GKGCMC 1 cut(s) 511
BalI TGGCCA 1 cut(s) 786
Bbv12I GWGCWC 2 cut(s) 336, 511
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 4 cut(s) 54, 261, 288, 815
BceAI ACGGC 2 cut(s) 224, 1337
BciT130I CCWGG 1 cut(s) 1086
BciVI GTATCC 1 cut(s) 527
BcnI CCSGG 2 cut(s) 1040, 1314
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 1 cut(s) 297
BfmI CTRYAG 1 cut(s) 124
BfoI RGCGCY 1 cut(s) 476
BfuI GTATCC 1 cut(s) 527
BlpI GCTNAGC 1 cut(s) 1176
Bme1390I CCNGG 3 cut(s) 1040, 1086, 1314
Bme18I GGWCC 1 cut(s) 1082
BmeT110I CYCGRG 1 cut(s) 1354
BmgT120I GGNCC 1 cut(s) 1082
BmrFI CCNGG 3 cut(s) 1040, 1086, 1314
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 3 cut(s) 505, 866, 1180
BmuI ACTGGG 1 cut(s) 1167
BpmI CTGGAG 1 cut(s) 1004
Bpu10I CCTNAGC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 1176
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 2 cut(s) 1040, 1314
BsaJI CCNNGG 2 cut(s) 540, 1085
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 4 cut(s) 1006, 1045, 1102, 1320
Bse1I ACTGG 4 cut(s) 417, 841, 1021, 1162
Bse21I CCTNAGG 1 cut(s) 1264
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 1 cut(s) 1086
BseDI CCNNGG 2 cut(s) 540, 1085
BseGI GGATG 3 cut(s) 520, 826, 1213
BseLI CCNNNNNNNGG 4 cut(s) 1006, 1045, 1102, 1320
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 3 cut(s) 108, 462, 741
BseNI ACTGG 4 cut(s) 417, 841, 1021, 1162
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 3 cut(s) 61, 177, 827
BshFI GGCC 1 cut(s) 786
BsiHKAI GWGCWC 2 cut(s) 336, 511
BsiHKCI CYCGRG 1 cut(s) 1354
BsiSI CCGG 3 cut(s) 512, 1040, 1314
BslI CCNNNNNNNGG 4 cut(s) 1006, 1045, 1102, 1320
BsmAI GTCTC 1 cut(s) 762
BsnI GGCC 1 cut(s) 786
BsoBI CYCGRG 1 cut(s) 1354
Bsp1286I GDGCHC 2 cut(s) 336, 511
Bsp143I GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
Bsp1720I GCTNAGC 1 cut(s) 1176
BspACI CCGC 6 cut(s) 37, 159, 177, 414, 827, 854
BspANI GGCC 1 cut(s) 786
BspCNI CTCAG 3 cut(s) 107, 461, 742
BspFNI CGCG 3 cut(s) 61, 177, 827
BspHI TCATGA 2 cut(s) 810, 1345
BspMAI CTGCAG 1 cut(s) 128
BspPI GGATC 5 cut(s) 785, 893, 916, 949, 1291
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 996
BsrI ACTGG 4 cut(s) 417, 841, 1021, 1162
BssECI CCNNGG 2 cut(s) 540, 1085
BssMI GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
BssNAI GTATAC 1 cut(s) 1319
BssT1I CCWWGG 1 cut(s) 540
Bst1107I GTATAC 1 cut(s) 1319
Bst2UI CCWGG 1 cut(s) 1086
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 2 cut(s) 714, 1242
BstAPI GCANNNNNTGC 2 cut(s) 461, 515
BstC8I GCNNGC 3 cut(s) 199, 507, 972
BstDEI CTNAG 5 cut(s) 94, 448, 750, 1176, 1264
BstF5I GGATG 3 cut(s) 520, 826, 1213
BstFNI CGCG 3 cut(s) 61, 177, 827
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 4 cut(s) 63, 177, 475, 827
BstKTI GATC 6 cut(s) 8, 793, 901, 911, 957, 1299
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
BstMWI GCNNNNNNNGC 7 cut(s) 217, 461, 470, 479, 515, 725, 851
BstNI CCWGG 1 cut(s) 1086
BstNSI RCATGY 2 cut(s) 974, 1328
BstSCI CCNGG 3 cut(s) 1038, 1084, 1312
BstSFI CTRYAG 1 cut(s) 124
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 3 cut(s) 61, 177, 827
BstX2I RGATCY 1 cut(s) 1296
BstXI CCANNNNNNTGG 2 cut(s) 530, 1157
BstYI RGATCY 1 cut(s) 1296
BstZ17I GTATAC 1 cut(s) 1319
Bsu36I CCTNAGG 1 cut(s) 1264
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 1 cut(s) 786
BtgZI GCGATG 2 cut(s) 32, 266
BtsCI GGATG 3 cut(s) 520, 826, 1213
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 1 cut(s) 876
Cac8I GCNNGC 3 cut(s) 199, 507, 972
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 2 cut(s) 810, 1345
CfoI GCGC 4 cut(s) 63, 177, 475, 827
Cfr13I GGNCC 1 cut(s) 1082
CspCI CAANNNNNGTGG 4 cut(s) 611, 646, 784, 819
DdeI CTNAG 5 cut(s) 94, 448, 750, 1176, 1264
DpnI GATC 6 cut(s) 7, 792, 900, 910, 956, 1298
DpnII GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 2 cut(s) 714, 1242
EarI CTCTTC 2 cut(s) 714, 1242
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 540
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 1082
Eco57I CTGAAG 1 cut(s) 456
Eco81I CCTNAGG 1 cut(s) 1264
Eco88I CYCGRG 1 cut(s) 1354
EcoRII CCWGG 1 cut(s) 1084
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 540
FblI GTMKAC 2 cut(s) 1318, 1329
FokI GGATG 3 cut(s) 527, 833, 1220
FspBI CTAG 1 cut(s) 297
GlaI GCGC 4 cut(s) 62, 176, 474, 826
GsuI CTGGAG 1 cut(s) 1004
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 1 cut(s) 786
HapII CCGG 3 cut(s) 512, 1040, 1314
HhaI GCGC 4 cut(s) 63, 177, 475, 827
Hin6I GCGC 4 cut(s) 61, 175, 473, 825
HinP1I GCGC 4 cut(s) 61, 175, 473, 825
HincII GTYRAC 1 cut(s) 1330
HindII GTYRAC 1 cut(s) 1330
HinfI GANTC 2 cut(s) 316, 1049
HpaII CCGG 3 cut(s) 512, 1040, 1314
HphI GGTGA 3 cut(s) 242, 1087, 1256
Hpy166II GTNNAC 6 cut(s) 250, 445, 509, 633, 1319, 1330
Hpy188I TCNGA 3 cut(s) 97, 954, 1097
Hpy188III TCNNGA 6 cut(s) 313, 733, 811, 1027, 1187, 1346
Hpy8I GTNNAC 6 cut(s) 250, 445, 509, 633, 1319, 1330
HpyAV CCTTC 3 cut(s) 692, 1006, 1198
HpyCH4III ACNGT 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 7 cut(s) 217, 461, 470, 479, 515, 725, 851
HpyF3I CTNAG 5 cut(s) 94, 448, 750, 1176, 1264
HspAI GCGC 4 cut(s) 61, 175, 473, 825
Kzo9I GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
LmnI GCTCC 2 cut(s) 148, 158
LweI GCATC 3 cut(s) 505, 866, 1180
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 3 cut(s) 349, 832, 1255
MalI GATC 6 cut(s) 7, 792, 900, 910, 956, 1298
MbiI CCGCTC 1 cut(s) 161
MboI GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
MboII GAAGA 5 cut(s) 225, 701, 1084, 1178, 1259
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 1 cut(s) 1296
MhlI GDGCHC 2 cut(s) 336, 511
MlsI TGGCCA 1 cut(s) 786
MluNI TGGCCA 1 cut(s) 786
MmeI TCCRAC 1 cut(s) 1266
MnlI CCTC 9 cut(s) 22, 44, 166, 657, 745, 811, 1066, 1243, 1350
Mox20I TGGCCA 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 441
MscI TGGCCA 1 cut(s) 786
MseI TTAA 2 cut(s) 1101, 1224
MslI CAYNNNNRTG 3 cut(s) 364, 528, 1337
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 746
MspI CCGG 3 cut(s) 512, 1040, 1314
MspR9I CCNGG 3 cut(s) 1040, 1086, 1314
MunI CAATTG 1 cut(s) 912
MvaI CCWGG 1 cut(s) 1086
MvnI CGCG 3 cut(s) 61, 177, 827
MwoI GCNNNNNNNGC 7 cut(s) 217, 461, 470, 479, 515, 725, 851
NciI CCSGG 2 cut(s) 1040, 1314
NdeII GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
NmuCI GTSAC 2 cut(s) 349, 1255
NspI RCATGY 2 cut(s) 974, 1328
PaeI GCATGC 1 cut(s) 974
PaeR7I CTCGAG 1 cut(s) 1354
PagI TCATGA 2 cut(s) 810, 1345
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 316, 1049
PflMI CCANNNNNTGG 1 cut(s) 1006
Psp6I CCWGG 1 cut(s) 1084
PspGI CCWGG 1 cut(s) 1084
PspPI GGNCC 1 cut(s) 1082
PspXI VCTCGAGB 1 cut(s) 1354
PstI CTGCAG 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 1296
PvuII CAGCTG 1 cut(s) 746
RseI CAYNNNNRTG 3 cut(s) 364, 528, 1337
SalI GTCGAC 1 cut(s) 1328
SaqAI TTAA 2 cut(s) 1101, 1224
Sau3AI GATC 6 cut(s) 5, 790, 898, 908, 954, 1296
Sau96I GGNCC 1 cut(s) 1082
ScrFI CCNGG 3 cut(s) 1040, 1086, 1314
SduI GDGCHC 2 cut(s) 336, 511
SfaNI GCATC 3 cut(s) 505, 866, 1180
SfcI CTRYAG 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 1354
SinI GGWCC 1 cut(s) 1082
SlaI CTCGAG 1 cut(s) 1354
SmiMI CAYNNNNRTG 3 cut(s) 364, 528, 1337
SmlI CTYRAG 2 cut(s) 499, 1354
SmoI CTYRAG 2 cut(s) 499, 1354
SphI GCATGC 1 cut(s) 974
SsiI CCGC 6 cut(s) 37, 159, 177, 414, 827, 854
SspI AATATT 1 cut(s) 655
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 1038, 1084, 1312
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 1 cut(s) 338
TaqI TCGA 3 cut(s) 222, 1329, 1355
TauI GCSGC 2 cut(s) 830, 857
TfiI GAWTC 2 cut(s) 316, 1049
Tru1I TTAA 2 cut(s) 1101, 1224
Tru9I TTAA 2 cut(s) 1101, 1224
TscAI CASTG 1 cut(s) 876
TseFI GTSAC 2 cut(s) 349, 1255
Tsp45I GTSAC 2 cut(s) 349, 1255
TspDTI ATGAA 2 cut(s) 602, 773
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 1 cut(s) 876
Van91I CCANNNNNTGG 1 cut(s) 1006
VneI GTGCAC 1 cut(s) 507
VpaK11BI GGWCC 1 cut(s) 1082
XapI RAATTY 1 cut(s) 392
XceI RCATGY 2 cut(s) 974, 1328
XhoI CTCGAG 1 cut(s) 1354
XmiI GTMKAC 2 cut(s) 1318, 1329
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.