pycom17g13910

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000942
Physical Location & Seq
Reverse (-)
11513057 .. 11516554
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g13910.5

Sequence Viewer

Length: 1362 bp
ATGAGAACGTCCTCCTACACTCTCGTCTCCCGCCTCCGCCGCACCCACCGCTTCAGTCCTCGCTGCTCCTCCCAATGTCTGCTCTTTCACTACTCAGACCCCCGGAACGACGATCGCAGCCTCCCCCAAAAGGCTCCGTTTAGCTCCTCTGCTCACACTCTGCTGCGCGGCCTGGTTTCCTCAGCTGCCTGCTCGAAACGGGTCGTTTTGTTCGACTTGGTTTCGTCCGGTAGTGTTGTTCATCGTCGTCAGCAGCCGCAGCGGCACTTGCTCCATCGCAGTTTCTTTACTAGAGCTAAGCAGGTGAAGATTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTTGTCTTTTCACTCAAGTTCGGTGTCTGGTTATCCACCTCTAGCCATGTTATGTTTGCCGAAGTTGTGCACTCAGTTGCTGATTTTGCAAATCAGGCGCTTCTTGCTTATGGTTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTTATGGATACTCCAAGGAAAGATTTGTCTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCTGGTGCGACTATCATTCATGGAGTTCAAAACTTGTGGACTTCACAGCCCCCTCCAAATATTCAGTATGCAGCTCTTGTGATTGGTGGCTCATTTATTATTGAAGGTGCTTCTCTTCTTGTTGCCATACAAGCCGTCAAGAAAGGTGCAGCTGCTGAGGGCATGAAACTGAGAGACTATATATGGCGTGGACATGATCCCACATCTGTTGCAGTCATGACAGAGGATGGGGCAGCTGTAACTGGGCTTGCTATTGCTGCGGCATCATTGGTTGCAGTGAATAGAACAGGAAATGCAATTTATGATCCCATAGGATCGATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATTTTTCTAATCCAAAGGAATCGACATGCTTTGATTGGCAGGGCAATGGATGACCATGATATGGAGAAGGTTCTCCAATTTTTGAAAAATGACCCGGTTGTGGATTCTCTATATGATTGCAAAAGCGAGGTGATTGGTCCTGGGTTCTTCAGATTTAAGGCTGAAATAGATTTCAATGGAGTAGTGGTAGTTCAAAATTATCTCCATAGGACTGGGCGTGGAGAATGGGCAAGACAGTTTCGTGAGGCTGCAAAGGAGAAGGATGATACTGCATTGCTTAAAATGATGTCAAACTATGGTGAAGAGGTAGTGACAGCATTAGGAAGTGAAGTTGATAGATTGGAAAAGGAGATCCAAAAGCTTGTTCCTGGTATACGGCATGTTGATATCGAGGCGCACAATCCAACGGAAGTATCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

454

Amino Acids

50.12

Weight (kDa)

9.23

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 1 cut(s) 292
AccB7I CCANNNNNTGG 1 cut(s) 1000
AccI GTMKAC 1 cut(s) 1312
AccII CGCG 1 cut(s) 168
AciI CCGC 8 cut(s) 31, 37, 40, 49, 168, 257, 262, 848
AclWI GGATC 4 cut(s) 779, 887, 910, 1285
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 2 cut(s) 37, 1072
AfiI CCNNNNNNNGG 3 cut(s) 130, 1000, 1039
AgsI TTSAA 7 cut(s) 314, 319, 617, 692, 1024, 1114, 1133
AjnI CCWGG 3 cut(s) 171, 1078, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 1287, 1319
AluBI AGCT 8 cut(s) 144, 185, 296, 492, 662, 740, 824, 1300
AluI AGCT 8 cut(s) 144, 185, 296, 492, 662, 740, 824, 1300
Alw21I GWGCWC 2 cut(s) 441, 505
Alw26I GTCTC 2 cut(s) 31, 756
Alw44I GTGCAC 2 cut(s) 437, 501
AlwI GGATC 4 cut(s) 779, 887, 910, 1285
AlwNI CAGNNNCTG 2 cut(s) 449, 743
AoxI GGCC 1 cut(s) 169
ApaLI GTGCAC 2 cut(s) 437, 501
ApoI RAATTY 1 cut(s) 314
AspLEI GCGC 3 cut(s) 168, 469, 1336
AspS9I GGNCC 1 cut(s) 1076
AsuC2I CCSGG 2 cut(s) 103, 1034
AsuHPI GGTGA 3 cut(s) 316, 1081, 1250
AvaII GGWCC 1 cut(s) 1076
BaeGI GKGCMC 2 cut(s) 441, 505
BaeI ACNNNNGTAYC 1 cut(s) 1335
Bbv12I GWGCWC 2 cut(s) 441, 505
BbvCI CCTCAGC 2 cut(s) 181, 744
BccI CCATC 2 cut(s) 282, 809
BceAI ACGGC 2 cut(s) 707, 1331
BciT130I CCWGG 3 cut(s) 173, 1080, 1308
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 2 cut(s) 103, 1034
BcoDI GTCTC 2 cut(s) 31, 756
BfaI CTAG 2 cut(s) 291, 411
BfoI RGCGCY 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 292
BfuI GTATCC 1 cut(s) 521
BglI GCCNNNNNGGC 1 cut(s) 262
BlpI GCTNAGC 1 cut(s) 297
Bme1390I CCNGG 5 cut(s) 103, 173, 1034, 1080, 1308
Bme18I GGWCC 1 cut(s) 1076
BmgT120I GGNCC 1 cut(s) 1076
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 5 cut(s) 103, 173, 1034, 1080, 1308
BmrI ACTGGG 2 cut(s) 840, 1161
BmsI GCATC 2 cut(s) 499, 860
BmuI ACTGGG 2 cut(s) 840, 1161
Bpu10I CCTNAGC 2 cut(s) 181, 744
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 2 cut(s) 368, 478
BpuMI CCSGG 2 cut(s) 103, 1034
Bsa29I ATCGAT 1 cut(s) 905
BsaJI CCNNGG 3 cut(s) 101, 534, 1079
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 1110, 1140
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 1000, 1039
Bse1I ACTGG 2 cut(s) 835, 1156
Bse3DI GCAATG 2 cut(s) 990, 1211
BseBI CCWGG 3 cut(s) 173, 1080, 1308
BseCI ATCGAT 1 cut(s) 905
BseDI CCNNGG 3 cut(s) 101, 534, 1079
BseGI GGATG 3 cut(s) 820, 994, 1207
BseLI CCNNNNNNNGG 3 cut(s) 130, 1000, 1039
BseMI GCAATG 2 cut(s) 990, 1211
BseMII CTCAG 5 cut(s) 108, 195, 456, 735, 749
BseNI ACTGG 2 cut(s) 835, 1156
BseRI GAGGAG 2 cut(s) 58, 136
BseSI GKGCMC 2 cut(s) 441, 505
BsgI GTGCAG 1 cut(s) 756
Bsh1236I CGCG 1 cut(s) 168
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 905
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 2 cut(s) 441, 505
BsiSI CCGG 3 cut(s) 103, 228, 1034
BslI CCNNNNNNNGG 3 cut(s) 130, 1000, 1039
BsmAI GTCTC 2 cut(s) 31, 756
BsmBI CGTCTC 1 cut(s) 31
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 441, 505
Bsp143I GATC 5 cut(s) 112, 784, 892, 902, 1290
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 8 cut(s) 31, 37, 40, 49, 168, 257, 262, 848
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 5 cut(s) 107, 194, 455, 736, 750
BspDI ATCGAT 1 cut(s) 905
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 292
BspPI GGATC 4 cut(s) 779, 887, 910, 1285
BsrDI GCAATG 2 cut(s) 990, 1211
BsrI ACTGG 2 cut(s) 835, 1156
BssECI CCNNGG 3 cut(s) 101, 534, 1079
BssMI GATC 5 cut(s) 112, 784, 892, 902, 1290
BssNAI GTATAC 1 cut(s) 1313
BssT1I CCWWGG 1 cut(s) 534
Bst1107I GTATAC 1 cut(s) 1313
Bst2UI CCWGG 3 cut(s) 173, 1080, 1308
Bst4CI ACNGT 2 cut(s) 332, 1176
Bst6I CTCTTC 2 cut(s) 708, 1236
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 190, 501, 837
BstDEI CTNAG 6 cut(s) 94, 181, 297, 442, 744, 758
BstF5I GGATG 3 cut(s) 820, 994, 1207
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 3 cut(s) 168, 469, 1336
BstKTI GATC 5 cut(s) 115, 787, 895, 905, 1293
BstMAI GTCTC 2 cut(s) 31, 756
BstMBI GATC 5 cut(s) 112, 784, 892, 902, 1290
BstMCI CGRYCG 1 cut(s) 115
BstNI CCWGG 3 cut(s) 173, 1080, 1308
BstNSI RCATGY 2 cut(s) 968, 1322
BstSCI CCNGG 5 cut(s) 101, 171, 1032, 1078, 1306
BstSLI GKGCMC 2 cut(s) 441, 505
BstUI CGCG 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 1290
BstXI CCANNNNNNTGG 2 cut(s) 524, 1151
BstYI RGATCY 1 cut(s) 1290
BstZ17I GTATAC 1 cut(s) 1313
Bsu15I ATCGAT 1 cut(s) 905
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 905
BtgZI GCGATG 1 cut(s) 260
BtsCI GGATG 3 cut(s) 820, 994, 1207
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 1 cut(s) 870
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 3 cut(s) 190, 501, 837
CaiI CAGNNNCTG 2 cut(s) 449, 743
CciI TCATGA 1 cut(s) 804
CfoI GCGC 3 cut(s) 168, 469, 1336
Cfr13I GGNCC 1 cut(s) 1076
ClaI ATCGAT 1 cut(s) 905
CspCI CAANNNNNGTGG 4 cut(s) 605, 640, 778, 813
CviAII CATG 9 cut(s) 416, 608, 751, 782, 805, 965, 995, 1319, 1359
DdeI CTNAG 6 cut(s) 94, 181, 297, 442, 744, 758
DpnI GATC 5 cut(s) 114, 786, 894, 904, 1292
DpnII GATC 5 cut(s) 112, 784, 892, 902, 1290
Eam1104I CTCTTC 2 cut(s) 708, 1236
EarI CTCTTC 2 cut(s) 708, 1236
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 534
Eco32I GATATC 2 cut(s) 561, 1327
Eco47I GGWCC 1 cut(s) 1076
Eco57I CTGAAG 2 cut(s) 37, 1072
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 3 cut(s) 171, 1078, 1306
EcoRV GATATC 2 cut(s) 561, 1327
EcoT14I CCWWGG 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 9 cut(s) 419, 611, 754, 785, 808, 968, 998, 1322, 1362
FatI CATG 9 cut(s) 415, 607, 750, 781, 804, 964, 994, 1318, 1358
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1312
FokI GGATG 3 cut(s) 827, 1001, 1214
FspBI CTAG 2 cut(s) 291, 411
GlaI GCGC 3 cut(s) 167, 468, 1335
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 1 cut(s) 171
HapII CCGG 3 cut(s) 103, 228, 1034
HhaI GCGC 3 cut(s) 168, 469, 1336
Hin1II CATG 9 cut(s) 419, 611, 754, 785, 808, 968, 998, 1322, 1362
Hin6I GCGC 3 cut(s) 166, 467, 1334
HinP1I GCGC 3 cut(s) 166, 467, 1334
HindIII AAGCTT 1 cut(s) 1298
HinfI GANTC 2 cut(s) 958, 1043
HpaII CCGG 3 cut(s) 103, 228, 1034
HphI GGTGA 3 cut(s) 316, 1081, 1250
Hpy166II GTNNAC 5 cut(s) 439, 503, 627, 779, 1313
Hpy188I TCNGA 2 cut(s) 97, 1091
Hpy188III TCNNGA 3 cut(s) 727, 805, 1181
Hpy8I GTNNAC 5 cut(s) 439, 503, 627, 779, 1313
Hpy99I CGWCG 2 cut(s) 113, 249
HpyAV CCTTC 3 cut(s) 686, 1000, 1192
HpyCH4III ACNGT 2 cut(s) 332, 1176
HpyCH4IV ACGT 1 cut(s) 8
HpyF3I CTNAG 6 cut(s) 94, 181, 297, 442, 744, 758
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 9 cut(s) 419, 611, 754, 785, 808, 968, 998, 1322, 1362
HspAI GCGC 3 cut(s) 166, 467, 1334
Kzo9I GATC 5 cut(s) 112, 784, 892, 902, 1290
LmnI GCTCC 4 cut(s) 71, 139, 149, 276
LweI GCATC 2 cut(s) 499, 860
MaeI CTAG 2 cut(s) 291, 411
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 3 cut(s) 343, 826, 1249
MalI GATC 5 cut(s) 114, 786, 894, 904, 1292
MboI GATC 5 cut(s) 112, 784, 892, 902, 1290
MboII GAAGA 4 cut(s) 319, 695, 1078, 1253
MflI RGATCY 1 cut(s) 1290
MhlI GDGCHC 2 cut(s) 441, 505
MluCI AATT 4 cut(s) 314, 885, 1016, 1135
MseI TTAA 2 cut(s) 1095, 1218
MslI CAYNNNNRTG 2 cut(s) 358, 522
MspA1I CMGCKG 4 cut(s) 185, 262, 740, 824
MspI CCGG 3 cut(s) 103, 228, 1034
MspR9I CCNGG 5 cut(s) 103, 173, 1034, 1080, 1308
MvaI CCWGG 3 cut(s) 173, 1080, 1308
MvnI CGCG 1 cut(s) 168
NciI CCSGG 2 cut(s) 103, 1034
NdeII GATC 5 cut(s) 112, 784, 892, 902, 1290
NlaIII CATG 9 cut(s) 419, 611, 754, 785, 808, 968, 998, 1322, 1362
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 343, 1249
NspI RCATGY 2 cut(s) 968, 1322
PagI TCATGA 1 cut(s) 804
PaqCI CACCTGC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 2 cut(s) 958, 1043
PflMI CCANNNNNTGG 1 cut(s) 1000
Ple19I CGATCG 1 cut(s) 115
Psp6I CCWGG 3 cut(s) 171, 1078, 1306
PspGI CCWGG 3 cut(s) 171, 1078, 1306
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 1076
PsrI GAACNNNNNNTAC 2 cut(s) 1113, 1145
PstNI CAGNNNCTG 2 cut(s) 449, 743
PsuI RGATCY 1 cut(s) 1290
PvuI CGATCG 1 cut(s) 115
PvuII CAGCTG 3 cut(s) 185, 740, 824
RseI CAYNNNNRTG 2 cut(s) 358, 522
SaqAI TTAA 2 cut(s) 1095, 1218
Sau3AI GATC 5 cut(s) 112, 784, 892, 902, 1290
Sau96I GGNCC 1 cut(s) 1076
ScrFI CCNGG 5 cut(s) 103, 173, 1034, 1080, 1308
SduI GDGCHC 2 cut(s) 441, 505
SfaNI GCATC 2 cut(s) 499, 860
SinI GGWCC 1 cut(s) 1076
SmiMI CAYNNNNRTG 2 cut(s) 358, 522
SmlI CTYRAG 2 cut(s) 383, 493
SmoI CTYRAG 2 cut(s) 383, 493
Sse9I AATT 4 cut(s) 314, 885, 1016, 1135
SsiI CCGC 8 cut(s) 31, 37, 40, 49, 168, 257, 262, 848
SspI AATATT 1 cut(s) 649
SspMI CTAG 2 cut(s) 291, 411
StyD4I CCNGG 5 cut(s) 101, 171, 1032, 1078, 1306
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 332, 1176
TaiI ACGT 1 cut(s) 11
TaqI TCGA 5 cut(s) 194, 213, 905, 961, 1329
TasI AATT 4 cut(s) 314, 885, 1016, 1135
TauI GCSGC 5 cut(s) 42, 171, 259, 265, 851
TfiI GAWTC 2 cut(s) 958, 1043
Tru1I TTAA 2 cut(s) 1095, 1218
Tru9I TTAA 2 cut(s) 1095, 1218
TscAI CASTG 1 cut(s) 870
TseFI GTSAC 2 cut(s) 343, 1249
Tsp45I GTSAC 2 cut(s) 343, 1249
TspDTI ATGAA 3 cut(s) 230, 596, 767
TspGWI ACGGA 2 cut(s) 126, 1361
TspRI CASTG 1 cut(s) 870
Van91I CCANNNNNTGG 1 cut(s) 1000
VneI GTGCAC 2 cut(s) 437, 501
VpaK11BI GGWCC 1 cut(s) 1076
XapI RAATTY 1 cut(s) 314
XceI RCATGY 2 cut(s) 968, 1322
XmiI GTMKAC 1 cut(s) 1312
XspI CTAG 2 cut(s) 291, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.