Rh2BG487000

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000942
Physical Location & Seq
Reverse (-)
68697136 .. 68701314
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG487000.1

Sequence Viewer

Length: 1062 bp
ATGAGAACGTCGTCGTTTTCTCTCCTCTCTCGCCTCCGCCACTCCCGCCGCTGCTGCTACTCCTCCTCTAACTCTCTGCTTTTCCACTTGGCCCATCAAAACGACGATCGCCGCTCCTCCCAAAACGCGCCGTTTAGCTCCTCCTCCTTTCACTCCTACTCCACTCACACTCACACTCAGTTGCAGCGGCTCTTCTCCTCAGCTGCCTCCTCCAAACGGTTCGTTTTGCTCGACTTGCTTCCTTCCCCCGACGCTGTTAATCACCACTCGTTGCATCGAAGTTTCTTCACCAGAGCTAAACCTGTTAAACAAGTCAATCTTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTAGTGTTTTCGCTCAAGTTCACAGTCTGGTTATCCACCTCCAGCCATGTAATGTTGGCTGAAGTTGTGCATTCCGTTGCTGATTTTGCTAATCAGGCACTTCTTGCTTATGGGTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTATTCTATTAGGATTGCCTACTGAACGATACATAAATATACCGCATTATGTGAATGATGCATATAATATGATCAGATTCAATGGGTCAATGAATGCTAACCTGGTGGCTATCTTTCTGATACAAAGGAATCGACATGCTTTAATTGGTAGGGCAATGGATGACTATGATGTAGAGAAGGTTCTTCAGTTCTTGAAAAATGACCCGGTTGTAGATTCTTTATATGATTGCAAAAGCGAGGTGATTGGCCCGGGATTCTTCAGATTTAAGGCTGAGATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTACCTCTATAGGACTGGACGTCAAGAATGGGCAAGACAGTTTCGTGAAGCTGCAAAGGAGAAGGATGATGCTGCTTTGCTGAAAATGATGTCCAATTATGGTGAAGAAGTAGTTACAGCCTTGGGAAGTGAGGTTGATAGATTGGAAAAGGAGATTCAAGAGCTTGTTCCTGGTATACGGCATGTCGACATTGAGGCACACAATCCAATAGAGCTGTCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

353

Amino Acids

40.24

Weight (kDa)

8.3

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 119 - 177 1.8e-10 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 859
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 2 cut(s) 1012, 1023
AccII CGCG 1 cut(s) 128
AciI CCGC 6 cut(s) 37, 46, 49, 112, 187, 572
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 3 cut(s) 459, 698, 772
AcvI CACGTG 1 cut(s) 1059
AcyI GRCGYC 1 cut(s) 856
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 7 cut(s) 323, 328, 610, 724, 814, 833, 995
AhdI GACNNNNNGTC 1 cut(s) 855
AjnI CCWGG 2 cut(s) 630, 1006
AluBI AGCT 7 cut(s) 138, 203, 296, 501, 887, 1000, 1051
AluI AGCT 7 cut(s) 138, 203, 296, 501, 887, 1000, 1051
Alw21I GWGCWC 1 cut(s) 514
Alw44I GTGCAC 1 cut(s) 510
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 2 cut(s) 90, 775
ApaLI GTGCAC 1 cut(s) 510
ApeKI GCWGC 6 cut(s) 51, 54, 184, 203, 887, 908
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 91, 776
AsuC2I CCSGG 3 cut(s) 734, 779, 780
AsuHPI GGTGA 4 cut(s) 254, 280, 781, 950
AvaI CYCGRG 1 cut(s) 778
BaeGI GKGCMC 1 cut(s) 514
BbrPI CACGTG 1 cut(s) 1059
Bbv12I GWGCWC 1 cut(s) 514
BbvCI CCTCAGC 1 cut(s) 199
BbvI GCAGC 6 cut(s) 38, 41, 190, 196, 874, 895
BccI CCATC 1 cut(s) 102
BceAI ACGGC 2 cut(s) 115, 1031
BciT130I CCWGG 2 cut(s) 632, 1008
BclI TGATCA 1 cut(s) 600
BcnI CCSGG 3 cut(s) 734, 779, 780
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 844
BisI GCNGC 9 cut(s) 49, 52, 55, 112, 185, 188, 204, 888, 909
BlsI GCNGC 9 cut(s) 50, 53, 56, 113, 186, 189, 205, 889, 910
Bme1390I CCNGG 5 cut(s) 632, 734, 779, 780, 1008
BmeRI GACNNNNNGTC 1 cut(s) 855
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 2 cut(s) 91, 776
BmrFI CCNGG 5 cut(s) 632, 734, 779, 780, 1008
BmsI GCATC 4 cut(s) 283, 508, 577, 895
BpmI CTGGAG 1 cut(s) 403
Bpu10I CCTNAGC 1 cut(s) 199
BpuEI CTTGAG 2 cut(s) 377, 487
BpuMI CCSGG 3 cut(s) 734, 779, 780
BsaAI YACGTR 1 cut(s) 1059
BsaHI GRCGYC 1 cut(s) 856
BsaJI CCNNGG 2 cut(s) 778, 957
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 810, 840
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 1 cut(s) 856
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 2 cut(s) 632, 1008
BseDI CCNNGG 2 cut(s) 778, 957
BseGI GGATG 2 cut(s) 694, 907
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 3 cut(s) 191, 213, 792
BseNI ACTGG 1 cut(s) 856
BseRI GAGGAG 8 cut(s) 14, 52, 55, 106, 130, 133, 187, 199
BseSI GKGCMC 1 cut(s) 514
BseXI GCAGC 6 cut(s) 38, 41, 190, 196, 874, 895
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 92, 777
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 2 cut(s) 734, 779
BslI CCNNNNNNNGG 1 cut(s) 216
BsmI GAATGC 2 cut(s) 448, 628
BsnI GGCC 2 cut(s) 92, 777
BsoBI CYCGRG 1 cut(s) 778
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 2 cut(s) 106, 600
BspACI CCGC 6 cut(s) 37, 46, 49, 112, 187, 572
BspANI GGCC 2 cut(s) 92, 777
BspCNI CTCAG 3 cut(s) 190, 212, 793
BspFNI CGCG 1 cut(s) 128
BspQI GCTCTTC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 690
BsrI ACTGG 1 cut(s) 856
BssECI CCNNGG 2 cut(s) 778, 957
BssMI GATC 2 cut(s) 106, 600
BssNAI GTATAC 1 cut(s) 1013
BssNI GRCGYC 1 cut(s) 856
BssT1I CCWWGG 1 cut(s) 957
Bst1107I GTATAC 1 cut(s) 1013
Bst2UI CCWGG 2 cut(s) 632, 1008
Bst4CI ACNGT 4 cut(s) 219, 341, 403, 876
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 856
BstAPI GCANNNNNTGC 2 cut(s) 482, 518
BstBAI YACGTR 1 cut(s) 1059
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 3 cut(s) 177, 199, 801
BstF5I GGATG 2 cut(s) 694, 907
BstFNI CGCG 1 cut(s) 128
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 2 cut(s) 109, 603
BstMBI GATC 2 cut(s) 106, 600
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 7 cut(s) 45, 54, 235, 464, 473, 482, 518
BstNI CCWGG 2 cut(s) 632, 1008
BstNSI RCATGY 2 cut(s) 668, 1022
BstSCI CCNGG 5 cut(s) 630, 732, 777, 778, 1006
BstSFI CTRYAG 1 cut(s) 844
BstSLI GKGCMC 1 cut(s) 514
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 6 cut(s) 38, 41, 190, 196, 874, 895
BstZ17I GTATAC 1 cut(s) 1013
BsuRI GGCC 2 cut(s) 92, 777
BtsCI GGATG 2 cut(s) 694, 907
Cac8I GCNNGC 1 cut(s) 510
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 91, 776
Cfr9I CCCGGG 1 cut(s) 778
CseI GACGC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 630
CviAII CATG 3 cut(s) 425, 665, 1019
DdeI CTNAG 3 cut(s) 177, 199, 801
DpnI GATC 2 cut(s) 108, 602
DpnII GATC 2 cut(s) 106, 600
DriI GACNNNNNGTC 1 cut(s) 855
Eam1104I CTCTTC 1 cut(s) 197
Eam1105I GACNNNNNGTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 957
Eco57I CTGAAG 3 cut(s) 459, 698, 772
Eco72I CACGTG 1 cut(s) 1059
Eco88I CYCGRG 1 cut(s) 778
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 630, 1006
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 957
FaeI CATG 3 cut(s) 428, 668, 1022
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FatI CATG 3 cut(s) 424, 664, 1018
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 600
FblI GTMKAC 2 cut(s) 1012, 1023
Fnu4HI GCNGC 9 cut(s) 49, 52, 55, 112, 185, 188, 204, 888, 909
FokI GGATG 2 cut(s) 701, 914
Fsp4HI GCNGC 9 cut(s) 49, 52, 55, 112, 185, 188, 204, 888, 909
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 129
GluI GCNGC 9 cut(s) 49, 52, 55, 112, 185, 188, 204, 888, 909
GsuI CTGGAG 1 cut(s) 403
HaeIII GGCC 2 cut(s) 92, 777
HapII CCGG 2 cut(s) 734, 779
HgaI GACGC 1 cut(s) 260
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 856
Hin1II CATG 3 cut(s) 428, 668, 1022
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HinfI GANTC 5 cut(s) 606, 658, 743, 783, 991
HpaII CCGG 2 cut(s) 734, 779
HphI GGTGA 4 cut(s) 254, 280, 781, 950
Hpy166II GTNNAC 5 cut(s) 399, 512, 1013, 1024, 1056
Hpy188I TCNGA 3 cut(s) 605, 648, 791
Hpy188III TCNNGA 5 cut(s) 320, 721, 860, 881, 995
Hpy8I GTNNAC 5 cut(s) 399, 512, 1013, 1024, 1056
Hpy99I CGWCG 4 cut(s) 13, 16, 107, 254
HpyAV CCTTC 3 cut(s) 252, 700, 892
HpyCH4III ACNGT 4 cut(s) 219, 341, 403, 876
HpyCH4IV ACGT 3 cut(s) 8, 856, 1058
HpyCH4V TGCA 9 cut(s) 184, 274, 362, 372, 448, 512, 590, 759, 890
HpyF10VI GCNNNNNNNGC 7 cut(s) 45, 54, 235, 464, 473, 482, 518
HpyF3I CTNAG 3 cut(s) 177, 199, 801
HpySE526I ACGT 3 cut(s) 8, 856, 1058
Hsp92I GRCGYC 1 cut(s) 856
Hsp92II CATG 3 cut(s) 428, 668, 1022
HspAI GCGC 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 600
Kzo9I GATC 2 cut(s) 106, 600
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 2 cut(s) 119, 143
Lsp1109I GCAGC 6 cut(s) 38, 41, 190, 196, 874, 895
LweI GCATC 4 cut(s) 283, 508, 577, 895
MabI ACCWGGT 1 cut(s) 630
MaeI CTAG 1 cut(s) 380
MaeII ACGT 3 cut(s) 8, 856, 1058
MaeIII GTNAC 2 cut(s) 352, 949
MalI GATC 2 cut(s) 108, 602
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 106, 600
MboII GAAGA 5 cut(s) 184, 277, 704, 778, 953
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 4 cut(s) 323, 672, 835, 931
Mph1103I ATGCAT 1 cut(s) 592
MseI TTAA 4 cut(s) 258, 306, 671, 795
MslI CAYNNNNRTG 1 cut(s) 367
MspA1I CMGCKG 3 cut(s) 51, 187, 203
MspI CCGG 2 cut(s) 734, 779
MspR9I CCNGG 5 cut(s) 632, 734, 779, 780, 1008
Mva1269I GAATGC 2 cut(s) 448, 628
MvaI CCWGG 2 cut(s) 632, 1008
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 7 cut(s) 45, 54, 235, 464, 473, 482, 518
NciI CCSGG 3 cut(s) 734, 779, 780
NdeII GATC 2 cut(s) 106, 600
NlaIII CATG 3 cut(s) 428, 668, 1022
NmuCI GTSAC 1 cut(s) 352
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 2 cut(s) 668, 1022
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 2 cut(s) 448, 628
PfeI GAWTC 5 cut(s) 606, 658, 743, 783, 991
PkrI GCNGC 9 cut(s) 50, 53, 56, 113, 186, 189, 205, 889, 910
Ple19I CGATCG 1 cut(s) 109
PmaCI CACGTG 1 cut(s) 1059
PmlI CACGTG 1 cut(s) 1059
Ppu21I YACGTR 1 cut(s) 1059
Psp6I CCWGG 2 cut(s) 630, 1006
PspCI CACGTG 1 cut(s) 1059
PspGI CCWGG 2 cut(s) 630, 1006
PspPI GGNCC 2 cut(s) 91, 776
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PvuI CGATCG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 367
SalI GTCGAC 1 cut(s) 1022
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 4 cut(s) 258, 306, 671, 795
SatI GCNGC 9 cut(s) 49, 52, 55, 112, 185, 188, 204, 888, 909
Sau3AI GATC 2 cut(s) 106, 600
Sau96I GGNCC 2 cut(s) 91, 776
ScrFI CCNGG 5 cut(s) 632, 734, 779, 780, 1008
SduI GDGCHC 1 cut(s) 514
SexAI ACCWGGT 1 cut(s) 630
SfaNI GCATC 4 cut(s) 283, 508, 577, 895
SfcI CTRYAG 1 cut(s) 844
SmaI CCCGGG 1 cut(s) 780
SmiMI CAYNNNNRTG 1 cut(s) 367
SmlI CTYRAG 2 cut(s) 392, 502
SmoI CTYRAG 2 cut(s) 392, 502
Sse9I AATT 4 cut(s) 323, 672, 835, 931
SsiI CCGC 6 cut(s) 37, 46, 49, 112, 187, 572
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 5 cut(s) 630, 732, 777, 778, 1006
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 4 cut(s) 219, 341, 403, 876
TaiI ACGT 3 cut(s) 11, 859, 1061
TaqI TCGA 4 cut(s) 231, 277, 661, 1023
TasI AATT 4 cut(s) 323, 672, 835, 931
TauI GCSGC 3 cut(s) 51, 114, 190
TfiI GAWTC 5 cut(s) 606, 658, 743, 783, 991
Tru1I TTAA 4 cut(s) 258, 306, 671, 795
Tru9I TTAA 4 cut(s) 258, 306, 671, 795
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 6 cut(s) 51, 54, 184, 203, 887, 908
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 1 cut(s) 442
TspMI CCCGGG 1 cut(s) 778
VneI GTGCAC 1 cut(s) 510
XapI RAATTY 1 cut(s) 323
XceI RCATGY 2 cut(s) 668, 1022
XmaI CCCGGG 1 cut(s) 778
XmiI GTMKAC 2 cut(s) 1012, 1023
XspI CTAG 1 cut(s) 380
ZraI GACGTC 1 cut(s) 857
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.