Rroxscaffold_2G00117160

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000942
Physical Location & Seq
Forward (+)
44669527 .. 44671449
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117160.1

Sequence Viewer

Length: 486 bp
ATGTTAGTTGGGTTTTTGGTGGAATTGAGATTGGTTTATGGAAATGATTTGAGTTTTGATTACTTGGGTTTGTTATGGTTTTTGCAGCTTAATCTTGGTGTGGATGAGATCTATACTCTGTTTGTATTGAAGAAAGATGGGAAAGCTATTGTTGGGGAGGCCACGATTGAGGCAAACACGGTGTATGGTGCGTTGTGGGGGTTAGAGCCCCCTCCAAATATTCAATATGCAGATATAGTGATTGGTGGTTCGTTTATTATTGAAGGCACTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGTCATGACCGAGGATGGCGCTGCTGTAGCTGGTCTTGCTATTGCTGCAGCATCATTGCTTGCTGTAAATGTCACAGGAAATGGAATTTACTATCCTATAGGTTCATTTGTAGTTGGCAAGGATCTTGGAATGGTGGCTATACGAAATTGTTCCAGATTCACGGTTTCACTTACCATTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

161

Amino Acids

17.13

Weight (kDa)

4.53

Isoelectric Point (pI)

21.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycohydro_20b2 PF14845 29 - 69 9.6e-06 beta-acetyl hexosaminidase like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 432
AcsI RAATTY 1 cut(s) 387
AgsI TTSAA 3 cut(s) 130, 224, 263
AluBI AGCT 4 cut(s) 88, 146, 293, 332
AluI AGCT 4 cut(s) 88, 146, 293, 332
AlwI GGATC 1 cut(s) 432
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 4 cut(s) 85, 323, 347, 350
ApoI RAATTY 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 451
AspLEI GCGC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 210
BbvI GCAGC 4 cut(s) 97, 310, 334, 362
BccI CCATC 2 cut(s) 131, 311
BfaI CTAG 1 cut(s) 484
BfmI CTRYAG 3 cut(s) 327, 348, 399
BfoI RGCGCY 1 cut(s) 324
BglII AGATCT 1 cut(s) 108
BisI GCNGC 4 cut(s) 86, 324, 348, 351
BlsI GCNGC 4 cut(s) 87, 325, 349, 352
BmsI GCATC 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 312
Bse3DI GCAATG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 2 cut(s) 109, 322
BseMI GCAATG 1 cut(s) 356
BseXI GCAGC 4 cut(s) 97, 310, 334, 362
BshFI GGCC 1 cut(s) 161
BsnI GGCC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 108, 424
BspANI GGCC 1 cut(s) 161
BspHI TCATGA 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 2 cut(s) 108, 424
Bst4CI ACNGT 2 cut(s) 181, 466
Bst6I CTCTTC 1 cut(s) 279
BstC8I GCNNGC 1 cut(s) 363
BstF5I GGATG 2 cut(s) 109, 322
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 2 cut(s) 111, 427
BstMBI GATC 2 cut(s) 108, 424
BstMWI GCNNNNNNNGC 4 cut(s) 290, 329, 338, 347
BstSFI CTRYAG 3 cut(s) 327, 348, 399
BstV1I GCAGC 4 cut(s) 97, 310, 334, 362
BstX2I RGATCY 2 cut(s) 108, 424
BstYI RGATCY 2 cut(s) 108, 424
BsuRI GGCC 1 cut(s) 161
BtsCI GGATG 2 cut(s) 109, 322
Cac8I GCNNGC 1 cut(s) 363
CciI TCATGA 1 cut(s) 306
CfoI GCGC 1 cut(s) 323
CviAII CATG 1 cut(s) 307
CviJI RGCY 7 cut(s) 88, 146, 161, 208, 293, 332, 440
CviKI_1 RGCY 7 cut(s) 88, 146, 161, 208, 293, 332, 440
DpnI GATC 2 cut(s) 110, 426
DpnII GATC 2 cut(s) 108, 424
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 210
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 1 cut(s) 310
FatI CATG 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 86, 324, 348, 351
FokI GGATG 2 cut(s) 116, 329
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 4 cut(s) 86, 324, 348, 351
FspBI CTAG 1 cut(s) 484
GlaI GCGC 1 cut(s) 322
GluI GCNGC 4 cut(s) 86, 324, 348, 351
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 1 cut(s) 161
HhaI GCGC 1 cut(s) 323
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HinfI GANTC 1 cut(s) 459
Hpy188III TCNNGA 3 cut(s) 298, 307, 456
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 2 cut(s) 181, 466
HpyCH4V TGCA 3 cut(s) 85, 230, 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 290, 329, 338, 347
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 1 cut(s) 321
Kzo9I GATC 2 cut(s) 108, 424
LpnPI CCDG 3 cut(s) 318, 363, 469
Lsp1109I GCAGC 4 cut(s) 97, 310, 334, 362
LweI GCATC 1 cut(s) 362
MaeI CTAG 1 cut(s) 484
MaeIII GTNAC 1 cut(s) 373
MalI GATC 2 cut(s) 110, 426
MboI GATC 2 cut(s) 108, 424
MboII GAAGA 2 cut(s) 142, 266
MflI RGATCY 2 cut(s) 108, 424
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 3 cut(s) 23, 387, 448
MnlI CCTC 4 cut(s) 151, 163, 222, 307
MroXI GAANNNNTTC 1 cut(s) 451
MseI TTAA 1 cut(s) 90
MwoI GCNNNNNNNGC 4 cut(s) 290, 329, 338, 347
NdeII GATC 2 cut(s) 108, 424
NlaIII CATG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 373
PagI TCATGA 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 451
PfeI GAWTC 1 cut(s) 459
PkrI GCNGC 4 cut(s) 87, 325, 349, 352
PstI CTGCAG 1 cut(s) 352
PsuI RGATCY 2 cut(s) 108, 424
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 4 cut(s) 86, 324, 348, 351
Sau3AI GATC 2 cut(s) 108, 424
SduI GDGCHC 1 cut(s) 210
SetI ASST 5 cut(s) 90, 148, 295, 334, 406
SfaNI GCATC 1 cut(s) 362
SfcI CTRYAG 3 cut(s) 327, 348, 399
Sse9I AATT 3 cut(s) 23, 387, 448
SspI AATATT 1 cut(s) 220
SspMI CTAG 1 cut(s) 484
TaaI ACNGT 2 cut(s) 181, 466
TaqII GACCGA 1 cut(s) 326
TasI AATT 3 cut(s) 23, 387, 448
TfiI GAWTC 1 cut(s) 459
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 4 cut(s) 85, 323, 347, 350
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 396
XapI RAATTY 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 451
XspI CTAG 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.