FvH4_6g37160

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000942
Physical Location & Seq
Forward (+)
29238818 .. 29243487
4670 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37160.t1

Sequence Viewer

Length: 1347 bp
ATGAAAACGTCGTCGTTTTCTCTCCTCTCTCGCCTCCGCCACTCCCGCCGCTCCTCCTCCTCCTCAAAGTGTCTGCTTTTCCTCTACGACGATCGCCGCTCCTCCCAAAACGCACCGTTTGGCTCCTCCTCCTACTACTACTCCACTCAAACTCACACTCAGCTAAACCGGCTCTTCTCTTCAAAACGGTTCGTTTTGCTCGACTTGCTTCCTTCCCACGGTGTTCATCAGCAGAATCAGCTCCTCTTGCATCGAAATTTCTTGACCAGAGCTAAACCTGTTAAGCAAGTTAAAATTGAATTCAATGACCAGCACAGTCAAAGAGCAGTCACAACTGCATTGTGGTGCAACTTCTTAGTGTTTTCACTTAAGTTTACGGTTTGGTTATCCACCTCGAGCCATGTGATGTTTGCTGAGGTTGTGCACTCGGTTGCGGATTTCGCTAATCAGGCGCTTCTTGCTTACGGGTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTTATGGATACTCCAAAGAAAGATTTGTTTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCTGGTGCAACTATTGTTCATGGAGTTCAAAACTTGTGGGTTTCACAGCCCCCTCCAAATATTCAATATGCCGCTATAGTGATTGGTGGTTCATTTATAATTGAAGGCGCGTCTCTTCTTGTTGCCATCCAAGCAGTCAAGAAAGGTGCCGCTCAAGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATGATCCTACCGCTGTTGCAGTCATGACTGAGGATGGTGCCGCTGTAGCTGGTCTTGCTATTGCTGCAGCATCATTGGTTGCAACAAAAGTCACAGGAAATGGAATTTACGATCCTATAGGTTCAATTGTAGTTGGCAACCTACTCGGAATGGTGGCTATTTTTCTGATACAAAGGAATCGACATGCTTTGATTGGTAGGGCAATGGATGACCAAGATGTGGAGAAGGTTCTTCAATTCTTGAAAAATGACCCGGTTGTGGATTCTTTATATGATTGCAAAAGTGAGGTGATTGGCCCGGGATTCTTCAGATTCAAGGCTGAGATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTATCTCTATAGGACTGGACGTCAAGAATGGGCAAGACAGTTTCGTGAAGCTGCAAAGGAGAAAGACGATGCTGCTTTGCTGAAAATGATGTCCAATTATGGTGAAGAAGTAGTTACAGCCTTGGGGAGTGAAGTCGATAGATTGGAAAAGGAGATCCAAGAGCTTGTTCCTGGTATACGGCATGTCGACATAGAGGCACACAATCCAATAGAGCTAACCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

449

Amino Acids

49.73

Weight (kDa)

8.87

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 111 - 319 2.4e-31 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AatII GACGTC 1 cut(s) 1144
AccB1I GGYRCC 2 cut(s) 719, 803
AccB7I CCANNNNNTGG 1 cut(s) 985
AccBSI CCGCTC 3 cut(s) 51, 99, 725
AccI GTMKAC 2 cut(s) 1297, 1308
AccII CGCG 1 cut(s) 683
AciI CCGC 9 cut(s) 37, 46, 49, 97, 434, 645, 723, 777, 807
AclWI GGATC 3 cut(s) 764, 872, 1270
AcsI RAATTY 3 cut(s) 256, 299, 870
AcuI CTGAAG 1 cut(s) 1057
AcvI CACGTG 1 cut(s) 1344
AcyI GRCGYC 1 cut(s) 1141
AfiI CCNNNNNNNGG 3 cut(s) 218, 985, 1024
AflII CTTAAG 1 cut(s) 368
AhdI GACNNNNNGTC 1 cut(s) 1140
AjnI CCWGG 1 cut(s) 1291
AjuI GAANNNNNNNTTGG 2 cut(s) 1272, 1304
AluBI AGCT 8 cut(s) 163, 241, 272, 477, 815, 1172, 1285, 1336
AluI AGCT 8 cut(s) 163, 241, 272, 477, 815, 1172, 1285, 1336
Alw21I GWGCWC 2 cut(s) 426, 490
Alw26I GTCTC 2 cut(s) 690, 741
Alw44I GTGCAC 2 cut(s) 422, 486
AlwI GGATC 3 cut(s) 764, 872, 1270
Ama87I CYCGRG 2 cut(s) 394, 1063
AoxI GGCC 1 cut(s) 1060
ApaLI GTGCAC 2 cut(s) 422, 486
ApeKI GCWGC 4 cut(s) 830, 833, 1172, 1193
ApoI RAATTY 3 cut(s) 256, 299, 870
AspLEI GCGC 2 cut(s) 454, 683
AspS9I GGNCC 1 cut(s) 1061
AsuC2I CCSGG 3 cut(s) 1019, 1064, 1065
AsuHPI GGTGA 2 cut(s) 1066, 1235
AvaI CYCGRG 2 cut(s) 394, 1063
BaeGI GKGCMC 2 cut(s) 426, 490
BanI GGYRCC 2 cut(s) 719, 803
BbrPI CACGTG 1 cut(s) 1344
Bbv12I GWGCWC 2 cut(s) 426, 490
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 4 cut(s) 817, 845, 1159, 1180
BccI CCATC 2 cut(s) 707, 794
BceAI ACGGC 1 cut(s) 1316
BcgI CGANNNNNNTGC 2 cut(s) 892, 926
BciT130I CCWGG 1 cut(s) 1293
BciVI GTATCC 1 cut(s) 506
BcnI CCSGG 3 cut(s) 1019, 1064, 1065
BcoDI GTCTC 2 cut(s) 690, 741
BfmI CTRYAG 5 cut(s) 648, 810, 831, 882, 1129
BfoI RGCGCY 1 cut(s) 455
BfrI CTTAAG 1 cut(s) 368
BfuI GTATCC 1 cut(s) 506
BisI GCNGC 9 cut(s) 49, 97, 645, 723, 807, 831, 834, 1173, 1194
BlsI GCNGC 9 cut(s) 50, 98, 646, 724, 808, 832, 835, 1174, 1195
Bme1390I CCNGG 4 cut(s) 1019, 1064, 1065, 1293
BmeRI GACNNNNNGTC 1 cut(s) 1140
BmeT110I CYCGRG 2 cut(s) 394, 1063
BmgT120I GGNCC 1 cut(s) 1061
BmiI GGNNCC 3 cut(s) 124, 721, 805
BmrFI CCNGG 4 cut(s) 1019, 1064, 1065, 1293
BmsI GCATC 4 cut(s) 259, 484, 845, 1180
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 463, 711
BpuMI CCSGG 3 cut(s) 1019, 1064, 1065
BsaAI YACGTR 1 cut(s) 1344
BsaHI GRCGYC 1 cut(s) 1141
BsaJI CCNNGG 3 cut(s) 217, 1063, 1242
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 1095, 1125
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 985, 1024
Bse118I RCCGGY 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 1141
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 1 cut(s) 1293
BseDI CCNNGG 3 cut(s) 217, 1063, 1242
BseGI GGATG 3 cut(s) 699, 805, 979
BseLI CCNNNNNNNGG 3 cut(s) 218, 985, 1024
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 4 cut(s) 173, 405, 786, 1077
BseNI ACTGG 1 cut(s) 1141
BseRI GAGGAG 9 cut(s) 14, 43, 46, 49, 52, 91, 115, 118, 233
BseSI GKGCMC 2 cut(s) 426, 490
BseXI GCAGC 4 cut(s) 817, 845, 1159, 1180
Bsh1236I CGCG 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 1062
BshNI GGYRCC 2 cut(s) 719, 803
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 426, 490
BsiHKCI CYCGRG 2 cut(s) 394, 1063
BsiSI CCGG 3 cut(s) 169, 1019, 1064
BslI CCNNNNNNNGG 3 cut(s) 218, 985, 1024
BsmAI GTCTC 2 cut(s) 690, 741
BsmBI CGTCTC 1 cut(s) 690
BsnI GGCC 1 cut(s) 1062
BsoBI CYCGRG 2 cut(s) 394, 1063
Bsp1286I GDGCHC 2 cut(s) 426, 490
Bsp143I GATC 4 cut(s) 91, 769, 877, 1275
BspACI CCGC 9 cut(s) 37, 46, 49, 97, 434, 645, 723, 777, 807
BspANI GGCC 1 cut(s) 1062
BspCNI CTCAG 4 cut(s) 172, 406, 787, 1078
BspFNI CGCG 1 cut(s) 683
BspHI TCATGA 2 cut(s) 766, 789
BspLI GGNNCC 3 cut(s) 124, 721, 805
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 3 cut(s) 764, 872, 1270
BspQI GCTCTTC 1 cut(s) 179
BspT107I GGYRCC 2 cut(s) 719, 803
BspTI CTTAAG 1 cut(s) 368
BsrBI CCGCTC 3 cut(s) 51, 99, 725
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 1 cut(s) 168
BsrI ACTGG 1 cut(s) 1141
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 3 cut(s) 217, 1063, 1242
BssMI GATC 4 cut(s) 91, 769, 877, 1275
BssNAI GTATAC 1 cut(s) 1298
BssNI GRCGYC 1 cut(s) 1141
BssT1I CCWWGG 1 cut(s) 1242
Bst1107I GTATAC 1 cut(s) 1298
Bst2UI CCWGG 1 cut(s) 1293
Bst4CI ACNGT 6 cut(s) 117, 189, 221, 317, 379, 1161
Bst6I CTCTTC 3 cut(s) 179, 184, 693
BstACI GRCGYC 1 cut(s) 1141
BstAFI CTTAAG 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 1 cut(s) 1344
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 5 cut(s) 159, 355, 414, 795, 1086
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 3 cut(s) 699, 805, 979
BstFNI CGCG 1 cut(s) 683
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 454, 683
BstKTI GATC 4 cut(s) 94, 772, 880, 1278
BstMAI GTCTC 2 cut(s) 690, 741
BstMBI GATC 4 cut(s) 91, 769, 877, 1275
BstMCI CGRYCG 1 cut(s) 94
BstNI CCWGG 1 cut(s) 1293
BstNSI RCATGY 2 cut(s) 953, 1307
BstSCI CCNGG 4 cut(s) 1017, 1062, 1063, 1291
BstSFI CTRYAG 5 cut(s) 648, 810, 831, 882, 1129
BstSLI GKGCMC 2 cut(s) 426, 490
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 4 cut(s) 817, 845, 1159, 1180
BstX2I RGATCY 1 cut(s) 1275
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 1 cut(s) 1275
BstZ17I GTATAC 1 cut(s) 1298
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 1 cut(s) 1062
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 699, 805, 979
Cac8I GCNNGC 1 cut(s) 486
CciI TCATGA 2 cut(s) 766, 789
CfoI GCGC 2 cut(s) 454, 683
Cfr10I RCCGGY 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 1061
Cfr9I CCCGGG 1 cut(s) 1063
CseI GACGC 1 cut(s) 672
CspCI CAANNNNNGTGG 2 cut(s) 590, 625
CviAII CATG 7 cut(s) 401, 593, 736, 767, 790, 950, 1304
DdeI CTNAG 5 cut(s) 159, 355, 414, 795, 1086
DpnI GATC 4 cut(s) 93, 771, 879, 1277
DpnII GATC 4 cut(s) 91, 769, 877, 1275
DriI GACNNNNNGTC 1 cut(s) 1140
Eam1104I CTCTTC 3 cut(s) 179, 184, 693
Eam1105I GACNNNNNGTC 1 cut(s) 1140
EarI CTCTTC 3 cut(s) 179, 184, 693
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 1242
Eco32I GATATC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 1057
Eco72I CACGTG 1 cut(s) 1344
Eco88I CYCGRG 2 cut(s) 394, 1063
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 1291
EcoRV GATATC 1 cut(s) 546
EcoT14I CCWWGG 1 cut(s) 1242
ErhI CCWWGG 1 cut(s) 1242
Esp3I CGTCTC 1 cut(s) 690
FaeI CATG 7 cut(s) 404, 596, 739, 770, 793, 953, 1307
FatI CATG 7 cut(s) 400, 592, 735, 766, 789, 949, 1303
FauI CCCGC 1 cut(s) 53
FblI GTMKAC 2 cut(s) 1297, 1308
Fnu4HI GCNGC 9 cut(s) 49, 97, 645, 723, 807, 831, 834, 1173, 1194
FokI GGATG 3 cut(s) 686, 812, 986
Fsp4HI GCNGC 9 cut(s) 49, 97, 645, 723, 807, 831, 834, 1173, 1194
GlaI GCGC 2 cut(s) 453, 682
GluI GCNGC 9 cut(s) 49, 97, 645, 723, 807, 831, 834, 1173, 1194
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 1 cut(s) 1062
HapII CCGG 3 cut(s) 169, 1019, 1064
HgaI GACGC 1 cut(s) 672
HhaI GCGC 2 cut(s) 454, 683
Hin1I GRCGYC 1 cut(s) 1141
Hin1II CATG 7 cut(s) 404, 596, 739, 770, 793, 953, 1307
Hin6I GCGC 2 cut(s) 452, 681
HinP1I GCGC 2 cut(s) 452, 681
HincII GTYRAC 1 cut(s) 1309
HindII GTYRAC 1 cut(s) 1309
HinfI GANTC 5 cut(s) 235, 943, 1028, 1068, 1077
HpaII CCGG 3 cut(s) 169, 1019, 1064
HphI GGTGA 2 cut(s) 1066, 1235
Hpy166II GTNNAC 5 cut(s) 375, 424, 488, 1298, 1309
Hpy188I TCNGA 3 cut(s) 914, 933, 1076
Hpy188III TCNNGA 8 cut(s) 262, 712, 728, 767, 790, 1006, 1145, 1166
Hpy8I GTNNAC 5 cut(s) 375, 424, 488, 1298, 1309
Hpy99I CGWCG 3 cut(s) 13, 16, 92
HpyAV CCTTC 3 cut(s) 222, 671, 985
HpyCH4III ACNGT 6 cut(s) 117, 189, 221, 317, 379, 1161
HpyCH4IV ACGT 3 cut(s) 8, 1141, 1343
HpyF3I CTNAG 5 cut(s) 159, 355, 414, 795, 1086
HpySE526I ACGT 3 cut(s) 8, 1141, 1343
Hsp92I GRCGYC 1 cut(s) 1141
Hsp92II CATG 7 cut(s) 404, 596, 739, 770, 793, 953, 1307
HspAI GCGC 2 cut(s) 452, 681
Kzo9I GATC 4 cut(s) 91, 769, 877, 1275
LguI GCTCTTC 1 cut(s) 179
LmnI GCTCC 4 cut(s) 56, 104, 128, 246
Lsp1109I GCAGC 4 cut(s) 817, 845, 1159, 1180
LweI GCATC 4 cut(s) 259, 484, 845, 1180
MaeII ACGT 3 cut(s) 8, 1141, 1343
MaeIII GTNAC 3 cut(s) 328, 856, 1234
MalI GATC 4 cut(s) 93, 771, 879, 1277
MbiI CCGCTC 3 cut(s) 51, 99, 725
MboI GATC 4 cut(s) 91, 769, 877, 1275
MboII GAAGA 6 cut(s) 166, 171, 680, 989, 1063, 1238
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 1275
MhlI GDGCHC 2 cut(s) 426, 490
MseI TTAA 4 cut(s) 282, 291, 369, 743
MslI CAYNNNNRTG 2 cut(s) 343, 507
MspA1I CMGCKG 2 cut(s) 779, 809
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 3 cut(s) 169, 1019, 1064
MspR9I CCNGG 4 cut(s) 1019, 1064, 1065, 1293
MunI CAATTG 1 cut(s) 891
MvaI CCWGG 1 cut(s) 1293
MvnI CGCG 1 cut(s) 683
NciI CCSGG 3 cut(s) 1019, 1064, 1065
NdeII GATC 4 cut(s) 91, 769, 877, 1275
NlaIII CATG 7 cut(s) 404, 596, 739, 770, 793, 953, 1307
NlaIV GGNNCC 3 cut(s) 124, 721, 805
NmuCI GTSAC 2 cut(s) 328, 856
NspI RCATGY 2 cut(s) 953, 1307
PaeR7I CTCGAG 1 cut(s) 394
PagI TCATGA 2 cut(s) 766, 789
PciSI GCTCTTC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 5 cut(s) 235, 943, 1028, 1068, 1077
PflMI CCANNNNNTGG 1 cut(s) 985
PkrI GCNGC 9 cut(s) 50, 98, 646, 724, 808, 832, 835, 1174, 1195
Ple19I CGATCG 1 cut(s) 94
PmaCI CACGTG 1 cut(s) 1344
PmlI CACGTG 1 cut(s) 1344
Ppu21I YACGTR 1 cut(s) 1344
PsiI TTATAA 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 1291
PspCI CACGTG 1 cut(s) 1344
PspGI CCWGG 1 cut(s) 1291
PspN4I GGNNCC 3 cut(s) 124, 721, 805
PspPI GGNCC 1 cut(s) 1061
PspXI VCTCGAGB 1 cut(s) 394
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 1 cut(s) 1275
PvuI CGATCG 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 343, 507
SalI GTCGAC 1 cut(s) 1307
SapI GCTCTTC 1 cut(s) 179
SaqAI TTAA 4 cut(s) 282, 291, 369, 743
SatI GCNGC 9 cut(s) 49, 97, 645, 723, 807, 831, 834, 1173, 1194
Sau3AI GATC 4 cut(s) 91, 769, 877, 1275
Sau96I GGNCC 1 cut(s) 1061
ScrFI CCNGG 4 cut(s) 1019, 1064, 1065, 1293
SduI GDGCHC 2 cut(s) 426, 490
SfaNI GCATC 4 cut(s) 259, 484, 845, 1180
SfcI CTRYAG 5 cut(s) 648, 810, 831, 882, 1129
Sfr274I CTCGAG 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 394
SmaI CCCGGG 1 cut(s) 1065
SmiMI CAYNNNNRTG 2 cut(s) 343, 507
SmlI CTYRAG 4 cut(s) 368, 394, 478, 726
SmoI CTYRAG 4 cut(s) 368, 394, 478, 726
SsiI CCGC 9 cut(s) 37, 46, 49, 97, 434, 645, 723, 777, 807
SspI AATATT 1 cut(s) 634
StyD4I CCNGG 4 cut(s) 1017, 1062, 1063, 1291
StyI CCWWGG 1 cut(s) 1242
TaaI ACNGT 6 cut(s) 117, 189, 221, 317, 379, 1161
TaiI ACGT 3 cut(s) 11, 1144, 1346
TaqI TCGA 6 cut(s) 201, 253, 395, 946, 1257, 1308
TauI GCSGC 5 cut(s) 51, 99, 647, 725, 809
TfiI GAWTC 5 cut(s) 235, 943, 1028, 1068, 1077
Tru1I TTAA 4 cut(s) 282, 291, 369, 743
Tru9I TTAA 4 cut(s) 282, 291, 369, 743
TseFI GTSAC 2 cut(s) 328, 856
TseI GCWGC 4 cut(s) 830, 833, 1172, 1193
Tsp45I GTSAC 2 cut(s) 328, 856
TspDTI ATGAA 5 cut(s) 17, 215, 581, 654, 752
TspMI CCCGGG 1 cut(s) 1063
Van91I CCANNNNNTGG 1 cut(s) 985
Vha464I CTTAAG 1 cut(s) 368
VneI GTGCAC 2 cut(s) 422, 486
XapI RAATTY 3 cut(s) 256, 299, 870
XceI RCATGY 2 cut(s) 953, 1307
XhoI CTCGAG 1 cut(s) 394
XmaI CCCGGG 1 cut(s) 1063
XmiI GTMKAC 2 cut(s) 1297, 1308
ZraI GACGTC 1 cut(s) 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.