MD17G1146400.v1.1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000942
Physical Location & Seq
Reverse (-)
13260208 .. 13264263
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1146400.v1.1.491

Sequence Viewer

Length: 1371 bp
ATGAGAACGTCCTCCTACACTCTCGCCTCCCGCCTCCGCCGCACCCACCGCTTCAGTACTCGCTGCCCCTCCCAATTTCTGCTCTTTCACTACACAGACCCCCGGAACGACGATCGCAGCCTCCCCCAAAAGGCTCCGTTTAGCTCCTCTGCTCACACTCTTCTGCGCGGCCTGGTTTCCTCAGCTGCCTGCTCGAAACGGGTCGTTTTGCTCGACTTGGTTTCGTCGGGTAGTGTTGTTCATCGTCACCATCAGCAGCAGCAGCAGCAGCGGCACTTGCTTCATCGCAGTTTCTTTACTAGAGCTAAGCAGGTGAAGATTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACTACTGCATTGTGGTGCAACTTCCTTGTCTTTTCACTTAAGTTCGGTGTCTGGTTATCCACCTCTAGCCATGTTATGTTTGCTGAAGTTGTGCACTCGGTTGCTGATTTTGCAAATCAGGCGCTTCTTGCTTATGGTTTGAGTAGCTCAAGGCGTGCACCGGATGCTCTCCACCCTTATGGATACTCCAAGGAAAGATTTGTCTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCTGGTGCGACTATCATTCATGGAGTTCAAAACTTGTGGACTTCACAGCCCCCTCCAAATATTCAGTATGCAGCTCTTGTGATTGGTGGCTCATTTATTATTGAAGGTGCTTCTCTTCTTGTTGCCATACAAGCCGTCAAGAAAGGTGCAGCTGCTGAGGGCATGAAACTGAGAGACTATATATGGCGCGGACATGATCCCACATCTGTTGCAGTCATGACAGAGGATGGGGCAGCTGTAACTGGGCTTGCTATTGCTGCGGCATCATTGGTTGCAGTGAATAGAACAGGAAATGCAATTTATGATCCCATAGGATCGATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATTTTTCTGATCCAAAGGAATCGACATGCTTTGATTGGCAGGGCAATGGATGACCATGATATGGAGAAGGTTCTCCAATTTTTGAAAAATGACCCGGTTGTGGATTCTCTATATGATTGCAAAAGCGAGGTGATTGGTCCTGGGTTCTTCAGATTTAAGGCTGAAATAGATTTCAATGGAGTAGTGGTAGTTCAAAATTATCTCCATAGGACTGGGCGTGGAGAATGGGCAAGACAGTTTCGTGAAGCTGCAAAGGAGAAGGATGATACTGCATTGCTTAAAATGATGTCAAACTATGGTGAAGAGGTAGTGACAGCATTAGGAAGTGAAGTTGATAGATTGGAAAAGGAGATCCAAAAGCTTGTTCCTGGTATACGGCATGTTGATATCGAGGCACACAATCCAACAGAAGTATCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

457

Amino Acids

50.54

Weight (kDa)

9.19

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 119 - 327 3.5e-30 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 301
AccB7I CCANNNNNTGG 1 cut(s) 1009
AccI GTMKAC 1 cut(s) 1321
AccII CGCG 2 cut(s) 168, 786
AciI CCGC 8 cut(s) 31, 37, 40, 49, 168, 271, 786, 857
AclWI GGATC 5 cut(s) 788, 896, 919, 952, 1294
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 3 cut(s) 37, 459, 1081
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 3 cut(s) 130, 1009, 1048
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 7 cut(s) 323, 328, 626, 701, 1033, 1123, 1142
AjnI CCWGG 3 cut(s) 171, 1087, 1315
AjuI GAANNNNNNNTTGG 2 cut(s) 1296, 1328
AluBI AGCT 9 cut(s) 144, 185, 305, 501, 671, 749, 833, 1196, 1309
AluI AGCT 9 cut(s) 144, 185, 305, 501, 671, 749, 833, 1196, 1309
Alw21I GWGCWC 2 cut(s) 450, 514
Alw26I GTCTC 1 cut(s) 765
Alw44I GTGCAC 2 cut(s) 446, 510
AlwI GGATC 5 cut(s) 788, 896, 919, 952, 1294
AlwNI CAGNNNCTG 1 cut(s) 752
AoxI GGCC 1 cut(s) 169
ApaLI GTGCAC 2 cut(s) 446, 510
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 3 cut(s) 168, 478, 786
AspS9I GGNCC 1 cut(s) 1085
AsuC2I CCSGG 2 cut(s) 103, 1043
AsuHPI GGTGA 4 cut(s) 239, 325, 1090, 1259
AvaII GGWCC 1 cut(s) 1085
BaeGI GKGCMC 2 cut(s) 450, 514
BaeI ACNNNNGTAYC 1 cut(s) 1344
Bbv12I GWGCWC 2 cut(s) 450, 514
BbvCI CCTCAGC 2 cut(s) 181, 753
BccI CCATC 2 cut(s) 258, 818
BceAI ACGGC 2 cut(s) 716, 1340
BciT130I CCWGG 3 cut(s) 173, 1089, 1317
BciVI GTATCC 1 cut(s) 530
BcnI CCSGG 2 cut(s) 103, 1043
BcoDI GTCTC 1 cut(s) 765
BfaI CTAG 2 cut(s) 300, 420
BfoI RGCGCY 1 cut(s) 479
BfrI CTTAAG 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 530
BlpI GCTNAGC 1 cut(s) 306
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 5 cut(s) 103, 173, 1043, 1089, 1317
Bme18I GGWCC 1 cut(s) 1085
BmgT120I GGNCC 1 cut(s) 1085
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 5 cut(s) 103, 173, 1043, 1089, 1317
BmrI ACTGGG 2 cut(s) 849, 1170
BmsI GCATC 2 cut(s) 508, 869
BmuI ACTGGG 2 cut(s) 849, 1170
Bpu10I CCTNAGC 2 cut(s) 181, 753
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 487
BpuMI CCSGG 2 cut(s) 103, 1043
Bsa29I ATCGAT 1 cut(s) 914
BsaJI CCNNGG 3 cut(s) 101, 543, 1088
BsaWI WCCGGW 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 1119, 1149
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 1009, 1048
Bse1I ACTGG 2 cut(s) 844, 1165
Bse3DI GCAATG 2 cut(s) 999, 1220
BseBI CCWGG 3 cut(s) 173, 1089, 1317
BseCI ATCGAT 1 cut(s) 914
BseDI CCNNGG 3 cut(s) 101, 543, 1088
BseGI GGATG 4 cut(s) 523, 829, 1003, 1216
BseLI CCNNNNNNNGG 3 cut(s) 130, 1009, 1048
BseMI GCAATG 2 cut(s) 999, 1220
BseMII CTCAG 3 cut(s) 195, 744, 758
BseNI ACTGG 2 cut(s) 844, 1165
BseRI GAGGAG 1 cut(s) 136
BseSI GKGCMC 2 cut(s) 450, 514
BsgI GTGCAG 1 cut(s) 765
Bsh1236I CGCG 2 cut(s) 168, 786
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 914
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 2 cut(s) 450, 514
BsiSI CCGG 3 cut(s) 103, 515, 1043
BslI CCNNNNNNNGG 3 cut(s) 130, 1009, 1048
BsmAI GTCTC 1 cut(s) 765
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 450, 514
Bsp143I GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 8 cut(s) 31, 37, 40, 49, 168, 271, 786, 857
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 3 cut(s) 194, 745, 759
BspDI ATCGAT 1 cut(s) 914
BspFNI CGCG 2 cut(s) 168, 786
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 5 cut(s) 788, 896, 919, 952, 1294
BspTI CTTAAG 1 cut(s) 392
BsrDI GCAATG 2 cut(s) 999, 1220
BsrI ACTGG 2 cut(s) 844, 1165
BssECI CCNNGG 3 cut(s) 101, 543, 1088
BssMI GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
BssNAI GTATAC 1 cut(s) 1322
BssT1I CCWWGG 1 cut(s) 543
Bst1107I GTATAC 1 cut(s) 1322
Bst2UI CCWGG 3 cut(s) 173, 1089, 1317
Bst4CI ACNGT 2 cut(s) 341, 1185
Bst6I CTCTTC 3 cut(s) 165, 717, 1245
BstAFI CTTAAG 1 cut(s) 392
BstAPI GCANNNNNTGC 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 190, 510, 846
BstDEI CTNAG 4 cut(s) 181, 306, 753, 767
BstF5I GGATG 4 cut(s) 523, 829, 1003, 1216
BstFNI CGCG 2 cut(s) 168, 786
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 3 cut(s) 168, 478, 786
BstKTI GATC 6 cut(s) 115, 796, 904, 914, 960, 1302
BstMAI GTCTC 1 cut(s) 765
BstMBI GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
BstMCI CGRYCG 1 cut(s) 115
BstNI CCWGG 3 cut(s) 173, 1089, 1317
BstNSI RCATGY 2 cut(s) 977, 1331
BstSCI CCNGG 5 cut(s) 101, 171, 1041, 1087, 1315
BstSLI GKGCMC 2 cut(s) 450, 514
BstUI CGCG 2 cut(s) 168, 786
BstX2I RGATCY 1 cut(s) 1299
BstXI CCANNNNNNTGG 2 cut(s) 533, 1160
BstYI RGATCY 1 cut(s) 1299
BstZ17I GTATAC 1 cut(s) 1322
Bsu15I ATCGAT 1 cut(s) 914
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 914
BtgZI GCGATG 1 cut(s) 269
BtsCI GGATG 4 cut(s) 523, 829, 1003, 1216
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 1 cut(s) 879
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 3 cut(s) 190, 510, 846
CaiI CAGNNNCTG 1 cut(s) 752
CciI TCATGA 1 cut(s) 813
CfoI GCGC 3 cut(s) 168, 478, 786
Cfr13I GGNCC 1 cut(s) 1085
ClaI ATCGAT 1 cut(s) 914
Csp6I GTAC 1 cut(s) 57
CspCI CAANNNNNGTGG 4 cut(s) 614, 649, 787, 822
CviAII CATG 9 cut(s) 425, 617, 760, 791, 814, 974, 1004, 1328, 1368
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 4 cut(s) 181, 306, 753, 767
DpnI GATC 6 cut(s) 114, 795, 903, 913, 959, 1301
DpnII GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
Eam1104I CTCTTC 3 cut(s) 165, 717, 1245
EarI CTCTTC 3 cut(s) 165, 717, 1245
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 543
Eco32I GATATC 2 cut(s) 570, 1336
Eco47I GGWCC 1 cut(s) 1085
Eco57I CTGAAG 3 cut(s) 37, 459, 1081
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 171, 1087, 1315
EcoRV GATATC 2 cut(s) 570, 1336
EcoT14I CCWWGG 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 9 cut(s) 428, 620, 763, 794, 817, 977, 1007, 1331, 1371
FatI CATG 9 cut(s) 424, 616, 759, 790, 813, 973, 1003, 1327, 1367
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1321
FokI GGATG 4 cut(s) 530, 836, 1010, 1223
FspBI CTAG 2 cut(s) 300, 420
GlaI GCGC 3 cut(s) 167, 477, 785
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 1 cut(s) 171
HapII CCGG 3 cut(s) 103, 515, 1043
HhaI GCGC 3 cut(s) 168, 478, 786
Hin1II CATG 9 cut(s) 428, 620, 763, 794, 817, 977, 1007, 1331, 1371
Hin6I GCGC 3 cut(s) 166, 476, 784
HinP1I GCGC 3 cut(s) 166, 476, 784
HindIII AAGCTT 1 cut(s) 1307
HinfI GANTC 2 cut(s) 967, 1052
HpaII CCGG 3 cut(s) 103, 515, 1043
HphI GGTGA 4 cut(s) 239, 325, 1090, 1259
Hpy166II GTNNAC 4 cut(s) 448, 512, 636, 1322
Hpy188I TCNGA 2 cut(s) 957, 1100
Hpy188III TCNNGA 3 cut(s) 736, 814, 1190
Hpy8I GTNNAC 4 cut(s) 448, 512, 636, 1322
Hpy99I CGWCG 2 cut(s) 113, 229
HpyAV CCTTC 3 cut(s) 695, 1009, 1201
HpyCH4III ACNGT 2 cut(s) 341, 1185
HpyCH4IV ACGT 1 cut(s) 8
HpyF3I CTNAG 4 cut(s) 181, 306, 753, 767
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 9 cut(s) 428, 620, 763, 794, 817, 977, 1007, 1331, 1371
HspAI GCGC 3 cut(s) 166, 476, 784
Kzo9I GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
LmnI GCTCC 2 cut(s) 139, 149
LweI GCATC 2 cut(s) 508, 869
MaeI CTAG 2 cut(s) 300, 420
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 4 cut(s) 245, 352, 835, 1258
MalI GATC 6 cut(s) 114, 795, 903, 913, 959, 1301
MboI GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
MboII GAAGA 5 cut(s) 152, 328, 704, 1087, 1262
MflI RGATCY 1 cut(s) 1299
MhlI GDGCHC 2 cut(s) 450, 514
MluCI AATT 5 cut(s) 74, 323, 894, 1025, 1144
MseI TTAA 3 cut(s) 393, 1104, 1227
MslI CAYNNNNRTG 2 cut(s) 367, 531
MspA1I CMGCKG 4 cut(s) 185, 271, 749, 833
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 3 cut(s) 103, 515, 1043
MspR9I CCNGG 5 cut(s) 103, 173, 1043, 1089, 1317
MvaI CCWGG 3 cut(s) 173, 1089, 1317
MvnI CGCG 2 cut(s) 168, 786
NciI CCSGG 2 cut(s) 103, 1043
NdeII GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
NlaIII CATG 9 cut(s) 428, 620, 763, 794, 817, 977, 1007, 1331, 1371
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 3 cut(s) 245, 352, 1258
NspI RCATGY 2 cut(s) 977, 1331
PagI TCATGA 1 cut(s) 813
PaqCI CACCTGC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 2 cut(s) 967, 1052
PflMI CCANNNNNTGG 1 cut(s) 1009
Ple19I CGATCG 1 cut(s) 115
Psp6I CCWGG 3 cut(s) 171, 1087, 1315
PspGI CCWGG 3 cut(s) 171, 1087, 1315
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 1085
PsrI GAACNNNNNNTAC 2 cut(s) 1122, 1154
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 1 cut(s) 1299
PvuI CGATCG 1 cut(s) 115
PvuII CAGCTG 3 cut(s) 185, 749, 833
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 2 cut(s) 367, 531
SaqAI TTAA 3 cut(s) 393, 1104, 1227
Sau3AI GATC 6 cut(s) 112, 793, 901, 911, 957, 1299
Sau96I GGNCC 1 cut(s) 1085
ScaI AGTACT 1 cut(s) 58
ScrFI CCNGG 5 cut(s) 103, 173, 1043, 1089, 1317
SduI GDGCHC 2 cut(s) 450, 514
SfaNI GCATC 2 cut(s) 508, 869
SinI GGWCC 1 cut(s) 1085
SmiMI CAYNNNNRTG 2 cut(s) 367, 531
SmlI CTYRAG 2 cut(s) 392, 502
SmoI CTYRAG 2 cut(s) 392, 502
Sse9I AATT 5 cut(s) 74, 323, 894, 1025, 1144
SsiI CCGC 8 cut(s) 31, 37, 40, 49, 168, 271, 786, 857
SspI AATATT 1 cut(s) 658
SspMI CTAG 2 cut(s) 300, 420
StyD4I CCNGG 5 cut(s) 101, 171, 1041, 1087, 1315
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 2 cut(s) 341, 1185
TaiI ACGT 1 cut(s) 11
TaqI TCGA 5 cut(s) 194, 213, 914, 970, 1338
TasI AATT 5 cut(s) 74, 323, 894, 1025, 1144
TatI WGTACW 1 cut(s) 56
TauI GCSGC 4 cut(s) 42, 171, 274, 860
TfiI GAWTC 2 cut(s) 967, 1052
Tru1I TTAA 3 cut(s) 393, 1104, 1227
Tru9I TTAA 3 cut(s) 393, 1104, 1227
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 3 cut(s) 245, 352, 1258
Tsp45I GTSAC 3 cut(s) 245, 352, 1258
TspDTI ATGAA 4 cut(s) 230, 272, 605, 776
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 1 cut(s) 879
Van91I CCANNNNNTGG 1 cut(s) 1009
Vha464I CTTAAG 1 cut(s) 392
VneI GTGCAC 2 cut(s) 446, 510
VpaK11BI GGWCC 1 cut(s) 1085
XapI RAATTY 1 cut(s) 323
XceI RCATGY 2 cut(s) 977, 1331
XmiI GTMKAC 1 cut(s) 1321
XspI CTAG 2 cut(s) 300, 420
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.