Rroxscaffold_2G00097740

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000942
Physical Location & Seq
Forward (+)
18946147 .. 18950791
4645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097740.1

Sequence Viewer

Length: 1359 bp
ATGAGAACGTCGTCGTTTTCTCTCCTCTCTCGCCTCCGCCACTCCCGCCGCTACTCCTCCTCTAACTCTCTTCTTTTCCACTTGGCCCATCCAAACGACGATCGCCGCTTCTCCCAAAACGCGCCGTTTAGCTCCTCCTCCCACTCCACTCACACTCACACTCAGCTGCAGCGGCTCTTCTCCTCAGCTGCCTGCTCCAAACGGTTCGTTTTGGTCGACTTGCTTCCTTCCCCCCAGGACGCTGTTAATCACCACTTGTTGCATCGAAATTTCTTCACCAGAGCTAAACCTGTTAAACAGGTCAGGCTTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTAGTGTTTTCGCTCAAGTTCACAGTCTGGTTATCCACCTCCAGCCATGTCATGTTGGCTGAAGTTGTGCATTCCGTTGCTGATTTTGCTAATCAGGCACTTCTTGCTTATGGGTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTTACGGATACTCCAAGGAAAGATTTGTTTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCCGGTGCAACTATAGTTCATGGAGTTCAAAACTTGTGGGTTTCACAGCCCCCTCCAAATATTCAATATGCAGCTTTAGTGATTGGTGGTTCGTTTATTATTGAAGGCGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGGTGCTGCTGCTGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATGATCCTACCTCTGTTGCAGTCATGACTGAGGATGGCGCTGCTGTAGCTGGTCTTGCTATTGCTGCAGCATCATTGGTTGCTGTAAATGTCACAGGAAATGGAATTTACGATCCAATAGGTTCAATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATTTTTCTGATACAAAGGAATCGACATGCTTTGATTGGTAGGGCAATGGATGACCATGATGTGGAGAAGGTTCTTCAGTTCTTGAAAAATGACCCGGTTGTGGATTCTTTATATGATTGCAAAAGCGAGGTGATTGGCCCGGGATTCTTCAGATTTAAGGCTGAGATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTATCTCTATAGGACTGGACGTCAGGAATGGGCAAGACAGTTTCGTGAAGCTGCAAAGGAGAAGGATGATGCTGCTTTGCTGAAAATGATGTCCAATTATGGTGAAGAAGTAGTTACAGCCTTGGGAAGTGAAGTAGATAGATTGGAAAAGGAGATCCAAGAGCTTGTTCCTGGTATACGGCATGTCGACATTGAGGCACACAATCCAATAGAGCTGGCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

452

Amino Acids

49.99

Weight (kDa)

8.28

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 115 - 323 1.2e-30 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1156
AccB7I CCANNNNNTGG 1 cut(s) 997
AccI GTMKAC 3 cut(s) 216, 1309, 1320
AccII CGCG 1 cut(s) 122
AciI CCGC 5 cut(s) 37, 46, 49, 106, 172
AclWI GGATC 3 cut(s) 776, 884, 1282
AcoI YGGCCR 1 cut(s) 1350
AcsI RAATTY 3 cut(s) 268, 311, 882
AcuI CTGAAG 3 cut(s) 447, 995, 1069
AcvI CACGTG 1 cut(s) 1356
AcyI GRCGYC 1 cut(s) 1153
AfiI CCNNNNNNNGG 2 cut(s) 997, 1036
AgsI TTSAA 9 cut(s) 311, 316, 614, 650, 689, 903, 1021, 1111, 1130
AhdI GACNNNNNGTC 1 cut(s) 1152
AjnI CCWGG 2 cut(s) 234, 1303
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 1 cut(s) 753
Alw44I GTGCAC 1 cut(s) 498
AlwI GGATC 3 cut(s) 776, 884, 1282
Ama87I CYCGRG 1 cut(s) 1075
AoxI GGCC 3 cut(s) 84, 1072, 1350
ApaLI GTGCAC 1 cut(s) 498
ApoI RAATTY 3 cut(s) 268, 311, 882
AspLEI GCGC 3 cut(s) 124, 695, 818
AspS9I GGNCC 2 cut(s) 85, 1073
AsuC2I CCSGG 3 cut(s) 1031, 1076, 1077
AsuHPI GGTGA 4 cut(s) 242, 268, 1078, 1247
AvaI CYCGRG 1 cut(s) 1075
BaeGI GKGCMC 1 cut(s) 502
BalI TGGCCA 1 cut(s) 1352
BbrPI CACGTG 1 cut(s) 1356
Bbv12I GWGCWC 1 cut(s) 502
BbvCI CCTCAGC 2 cut(s) 184, 741
BccI CCATC 2 cut(s) 96, 806
BceAI ACGGC 2 cut(s) 109, 1328
BciT130I CCWGG 2 cut(s) 236, 1305
BciVI GTATCC 1 cut(s) 518
BcnI CCSGG 3 cut(s) 1031, 1076, 1077
BcoDI GTCTC 1 cut(s) 753
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 5 cut(s) 167, 597, 822, 843, 1141
BfoI RGCGCY 2 cut(s) 696, 819
BfuI GTATCC 1 cut(s) 518
Bme1390I CCNGG 5 cut(s) 236, 1031, 1076, 1077, 1305
BmeRI GACNNNNNGTC 1 cut(s) 1152
BmeT110I CYCGRG 1 cut(s) 1075
BmgT120I GGNCC 2 cut(s) 85, 1073
BmiI GGNNCC 1 cut(s) 586
BmrFI CCNGG 5 cut(s) 236, 1031, 1076, 1077, 1305
BmsI GCATC 4 cut(s) 271, 496, 857, 1192
BpmI CTGGAG 1 cut(s) 391
Bpu10I CCTNAGC 2 cut(s) 184, 741
BpuEI CTTGAG 2 cut(s) 365, 475
BpuMI CCSGG 3 cut(s) 1031, 1076, 1077
BsaAI YACGTR 1 cut(s) 1356
BsaHI GRCGYC 1 cut(s) 1153
BsaJI CCNNGG 4 cut(s) 234, 531, 1075, 1254
BsaWI WCCGGW 1 cut(s) 587
BsaXI ACNNNNNCTCC 4 cut(s) 512, 542, 1107, 1137
Bsc4I CCNNNNNNNGG 2 cut(s) 997, 1036
Bse1I ACTGG 1 cut(s) 1153
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 2 cut(s) 236, 1305
BseDI CCNNGG 4 cut(s) 234, 531, 1075, 1254
BseGI GGATG 4 cut(s) 88, 817, 991, 1204
BseLI CCNNNNNNNGG 2 cut(s) 997, 1036
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 5 cut(s) 176, 198, 732, 798, 1089
BseNI ACTGG 1 cut(s) 1153
BseRI GAGGAG 6 cut(s) 14, 46, 49, 124, 127, 172
BseSI GKGCMC 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 3 cut(s) 86, 1074, 1352
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 1075
BsiSI CCGG 3 cut(s) 588, 1031, 1076
BslI CCNNNNNNNGG 2 cut(s) 997, 1036
BsmAI GTCTC 1 cut(s) 753
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 3 cut(s) 86, 1074, 1352
BsoBI CYCGRG 1 cut(s) 1075
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 4 cut(s) 100, 781, 889, 1287
BspACI CCGC 5 cut(s) 37, 46, 49, 106, 172
BspANI GGCC 3 cut(s) 86, 1074, 1352
BspCNI CTCAG 5 cut(s) 175, 197, 733, 799, 1090
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 2 cut(s) 778, 801
BspLI GGNNCC 1 cut(s) 586
BspMAI CTGCAG 2 cut(s) 171, 847
BspPI GGATC 3 cut(s) 776, 884, 1282
BspQI GCTCTTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 987
BsrI ACTGG 1 cut(s) 1153
BssECI CCNNGG 4 cut(s) 234, 531, 1075, 1254
BssMI GATC 4 cut(s) 100, 781, 889, 1287
BssNAI GTATAC 1 cut(s) 1310
BssNI GRCGYC 1 cut(s) 1153
BssT1I CCWWGG 2 cut(s) 531, 1254
Bst1107I GTATAC 1 cut(s) 1310
Bst2UI CCWGG 2 cut(s) 236, 1305
Bst4CI ACNGT 4 cut(s) 204, 329, 391, 1173
Bst6I CTCTTC 3 cut(s) 75, 182, 705
BstACI GRCGYC 1 cut(s) 1153
BstAPI GCANNNNNTGC 2 cut(s) 470, 506
BstBAI YACGTR 1 cut(s) 1356
BstC8I GCNNGC 3 cut(s) 193, 498, 1350
BstDEI CTNAG 5 cut(s) 162, 184, 741, 807, 1098
BstF5I GGATG 4 cut(s) 88, 817, 991, 1204
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 2 cut(s) 696, 819
BstHHI GCGC 3 cut(s) 124, 695, 818
BstKTI GATC 4 cut(s) 103, 784, 892, 1290
BstMAI GTCTC 1 cut(s) 753
BstMBI GATC 4 cut(s) 100, 781, 889, 1287
BstMCI CGRYCG 1 cut(s) 103
BstNI CCWGG 2 cut(s) 236, 1305
BstNSI RCATGY 2 cut(s) 965, 1319
BstSCI CCNGG 5 cut(s) 234, 1029, 1074, 1075, 1303
BstSFI CTRYAG 5 cut(s) 167, 597, 822, 843, 1141
BstSLI GKGCMC 1 cut(s) 502
BstUI CGCG 1 cut(s) 122
BstX2I RGATCY 1 cut(s) 1287
BstYI RGATCY 1 cut(s) 1287
BstZ17I GTATAC 1 cut(s) 1310
BsuI GTATCC 1 cut(s) 518
BsuRI GGCC 3 cut(s) 86, 1074, 1352
BtsCI GGATG 4 cut(s) 88, 817, 991, 1204
Cac8I GCNNGC 3 cut(s) 193, 498, 1350
CciI TCATGA 2 cut(s) 778, 801
CfoI GCGC 3 cut(s) 124, 695, 818
Cfr13I GGNCC 2 cut(s) 85, 1073
Cfr9I CCCGGG 1 cut(s) 1075
CseI GACGC 1 cut(s) 248
CspCI CAANNNNNGTGG 2 cut(s) 602, 637
CviAII CATG 9 cut(s) 413, 418, 605, 748, 779, 802, 962, 992, 1316
DdeI CTNAG 5 cut(s) 162, 184, 741, 807, 1098
DpnI GATC 4 cut(s) 102, 783, 891, 1289
DpnII GATC 4 cut(s) 100, 781, 889, 1287
DriI GACNNNNNGTC 1 cut(s) 1152
EaeI YGGCCR 1 cut(s) 1350
Eam1104I CTCTTC 3 cut(s) 75, 182, 705
Eam1105I GACNNNNNGTC 1 cut(s) 1152
EarI CTCTTC 3 cut(s) 75, 182, 705
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 531, 1254
Eco32I GATATC 1 cut(s) 558
Eco57I CTGAAG 3 cut(s) 447, 995, 1069
Eco72I CACGTG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 1075
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 234, 1303
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 2 cut(s) 531, 1254
ErhI CCWWGG 2 cut(s) 531, 1254
FaeI CATG 9 cut(s) 416, 421, 608, 751, 782, 805, 965, 995, 1319
FatI CATG 9 cut(s) 412, 417, 604, 747, 778, 801, 961, 991, 1315
FauI CCCGC 1 cut(s) 53
FblI GTMKAC 3 cut(s) 216, 1309, 1320
FokI GGATG 4 cut(s) 75, 824, 998, 1211
FspBI CTAG 1 cut(s) 368
GlaI GCGC 3 cut(s) 123, 694, 817
GsuI CTGGAG 1 cut(s) 391
HaeII RGCGCY 2 cut(s) 696, 819
HaeIII GGCC 3 cut(s) 86, 1074, 1352
HapII CCGG 3 cut(s) 588, 1031, 1076
HgaI GACGC 1 cut(s) 248
HhaI GCGC 3 cut(s) 124, 695, 818
Hin1I GRCGYC 1 cut(s) 1153
Hin1II CATG 9 cut(s) 416, 421, 608, 751, 782, 805, 965, 995, 1319
Hin6I GCGC 3 cut(s) 122, 693, 816
HinP1I GCGC 3 cut(s) 122, 693, 816
HincII GTYRAC 2 cut(s) 217, 1321
HindII GTYRAC 2 cut(s) 217, 1321
HinfI GANTC 3 cut(s) 955, 1040, 1080
HpaII CCGG 3 cut(s) 588, 1031, 1076
HphI GGTGA 4 cut(s) 242, 268, 1078, 1247
Hpy166II GTNNAC 5 cut(s) 217, 387, 500, 1310, 1321
Hpy188I TCNGA 2 cut(s) 945, 1088
Hpy188III TCNNGA 6 cut(s) 724, 779, 802, 1018, 1157, 1178
Hpy8I GTNNAC 5 cut(s) 217, 387, 500, 1310, 1321
Hpy99I CGWCG 3 cut(s) 13, 16, 101
HpyAV CCTTC 4 cut(s) 237, 683, 997, 1189
HpyCH4III ACNGT 4 cut(s) 204, 329, 391, 1173
HpyCH4IV ACGT 3 cut(s) 8, 1153, 1355
HpyF3I CTNAG 5 cut(s) 162, 184, 741, 807, 1098
HpySE526I ACGT 3 cut(s) 8, 1153, 1355
Hsp92I GRCGYC 1 cut(s) 1153
Hsp92II CATG 9 cut(s) 416, 421, 608, 751, 782, 805, 965, 995, 1319
HspAI GCGC 3 cut(s) 122, 693, 816
Kzo9I GATC 4 cut(s) 100, 781, 889, 1287
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 2 cut(s) 137, 200
LweI GCATC 4 cut(s) 271, 496, 857, 1192
MaeI CTAG 1 cut(s) 368
MaeII ACGT 3 cut(s) 8, 1153, 1355
MaeIII GTNAC 3 cut(s) 340, 868, 1246
MalI GATC 4 cut(s) 102, 783, 891, 1289
MboI GATC 4 cut(s) 100, 781, 889, 1287
MboII GAAGA 7 cut(s) 62, 169, 265, 692, 1001, 1075, 1250
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 1287
MhlI GDGCHC 1 cut(s) 502
MlsI TGGCCA 1 cut(s) 1352
MluCI AATT 7 cut(s) 268, 311, 752, 882, 903, 1132, 1228
MluNI TGGCCA 1 cut(s) 1352
Mox20I TGGCCA 1 cut(s) 1352
MscI TGGCCA 1 cut(s) 1352
MseI TTAA 4 cut(s) 246, 294, 755, 1092
MslI CAYNNNNRTG 1 cut(s) 355
Msp20I TGGCCA 1 cut(s) 1352
MspA1I CMGCKG 3 cut(s) 166, 172, 188
MspI CCGG 3 cut(s) 588, 1031, 1076
MspR9I CCNGG 5 cut(s) 236, 1031, 1076, 1077, 1305
MunI CAATTG 1 cut(s) 903
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 2 cut(s) 236, 1305
MvnI CGCG 1 cut(s) 122
NciI CCSGG 3 cut(s) 1031, 1076, 1077
NdeII GATC 4 cut(s) 100, 781, 889, 1287
NlaIII CATG 9 cut(s) 416, 421, 608, 751, 782, 805, 965, 995, 1319
NlaIV GGNNCC 1 cut(s) 586
NmuCI GTSAC 2 cut(s) 340, 868
NspI RCATGY 2 cut(s) 965, 1319
PagI TCATGA 2 cut(s) 778, 801
PciSI GCTCTTC 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 436
PfeI GAWTC 3 cut(s) 955, 1040, 1080
PflMI CCANNNNNTGG 1 cut(s) 997
Ple19I CGATCG 1 cut(s) 103
PmaCI CACGTG 1 cut(s) 1356
PmlI CACGTG 1 cut(s) 1356
Ppu21I YACGTR 1 cut(s) 1356
Psp6I CCWGG 2 cut(s) 234, 1303
PspCI CACGTG 1 cut(s) 1356
PspGI CCWGG 2 cut(s) 234, 1303
PspN4I GGNNCC 1 cut(s) 586
PspPI GGNCC 2 cut(s) 85, 1073
PstI CTGCAG 2 cut(s) 171, 847
PsuI RGATCY 1 cut(s) 1287
PvuI CGATCG 1 cut(s) 103
PvuII CAGCTG 2 cut(s) 166, 188
RseI CAYNNNNRTG 1 cut(s) 355
SalI GTCGAC 2 cut(s) 215, 1319
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 4 cut(s) 246, 294, 755, 1092
Sau3AI GATC 4 cut(s) 100, 781, 889, 1287
Sau96I GGNCC 2 cut(s) 85, 1073
ScrFI CCNGG 5 cut(s) 236, 1031, 1076, 1077, 1305
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 4 cut(s) 271, 496, 857, 1192
SfcI CTRYAG 5 cut(s) 167, 597, 822, 843, 1141
SmaI CCCGGG 1 cut(s) 1077
SmiMI CAYNNNNRTG 1 cut(s) 355
SmlI CTYRAG 2 cut(s) 380, 490
SmoI CTYRAG 2 cut(s) 380, 490
Sse9I AATT 7 cut(s) 268, 311, 752, 882, 903, 1132, 1228
SsiI CCGC 5 cut(s) 37, 46, 49, 106, 172
SspI AATATT 1 cut(s) 646
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 5 cut(s) 234, 1029, 1074, 1075, 1303
StyI CCWWGG 2 cut(s) 531, 1254
TaaI ACNGT 4 cut(s) 204, 329, 391, 1173
TaiI ACGT 3 cut(s) 11, 1156, 1358
TaqI TCGA 4 cut(s) 216, 265, 958, 1320
TasI AATT 7 cut(s) 268, 311, 752, 882, 903, 1132, 1228
TauI GCSGC 3 cut(s) 51, 108, 175
TfiI GAWTC 3 cut(s) 955, 1040, 1080
Tru1I TTAA 4 cut(s) 246, 294, 755, 1092
Tru9I TTAA 4 cut(s) 246, 294, 755, 1092
TseFI GTSAC 2 cut(s) 340, 868
Tsp45I GTSAC 2 cut(s) 340, 868
TspDTI ATGAA 2 cut(s) 593, 764
TspGWI ACGGA 2 cut(s) 430, 537
TspMI CCCGGG 1 cut(s) 1075
Van91I CCANNNNNTGG 1 cut(s) 997
VneI GTGCAC 1 cut(s) 498
XapI RAATTY 3 cut(s) 268, 311, 882
XceI RCATGY 2 cut(s) 965, 1319
XmaI CCCGGG 1 cut(s) 1075
XmiI GTMKAC 3 cut(s) 216, 1309, 1320
XspI CTAG 1 cut(s) 368
ZraI GACGTC 1 cut(s) 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.