Rh2CG461100

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000942
Physical Location & Seq
Reverse (-)
62409792 .. 62415006
5215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG461100.1

Sequence Viewer

Length: 1284 bp
ATGAGAACGTCGTCGTTTTCTCTCCTCTCTCGCCTCCGCCACTCCCGCCGCTGCTGCTACTCCTCTAACTCTCTGCTTTTCCACTTGGCCCATCAAAACGACGATCGCCGCTCCTCCCAAAACGCGCCGTTTAGCTCCTCCTCCTTTCACTCCTACTCCACTCACACTCAGTTGCAGCGGCTCTTCTCCTCAGCTGCCTCCTCCAAACGGTTCGTTTTGCTCGACTCGCTTCCTTCCCCCGACGCTGTTAATCACCACTTGTTGCATCGAAATTTCTTCACCAGAGCTAAACCTGTTAAACAAGTCAAGCTTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTAGTGTTTTCGCTCAAGTTCACAGTCTGGTTATCCACCTCCAGCCATGTAATGTTGGCTGAAGTTGTGCATTCCGTTGCTGATTTTGCTAATCAGGCACTTCTTGCTTATGGGTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTTACGGATACTCCAAGGAAAGATTTGTTTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCTGGTGCAACTATAGTTCATGGAGTTCAAAACTTGTGGGTTTCACAGCCCCCTCCAAATATTCAATATGCAGCTTTAGTGATTGGTGGTTCGTTTATTATTGAAGGCGCTTCTCTTCTTGTTGCCATACAAGCAGTCAAGAAAGGTGCTGCTGCTGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATGATCCTACCTCTGTTGCAGTCATGACTGAGGATGGCGCTGCTGTAGCTGGTCTTGCTATTGCTGCGGCATCATTGGTTGCTGTAAATGTCACAGGAAATGGAATTTACGATCCTATAGGTTCAATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATCTTTCTGATACAAAGGAATCGACATGCTTTAATTGGTAGGGCAATGGATGACTATGATGTAGAGAAGGTTCTTCAGTTCTTGAAAAATGACCCGGTTGTAGATTCTTTATATGATTGCAAAAGCGAGGTGATTGGCCCGGGATTCTTCAGATTTAAGGCTGAGATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTATCTCTATAGGACTGGACGTCAAGAATGGGCAAGACAGTTTCGTGAAGCTGCAAAGGAGAAGGATGATGCTGCTTTGCTGAAAATGATGTCCAATTATGGTATGCATTATGATTTTACTTATATTTATCATTATTTGGGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

427

Amino Acids

47.47

Weight (kDa)

9.05

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 116 - 324 1e-30 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1159
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 1 cut(s) 125
AciI CCGC 6 cut(s) 37, 46, 49, 109, 178, 848
AclWI GGATC 2 cut(s) 779, 887
AcsI RAATTY 3 cut(s) 271, 314, 885
AcuI CTGAAG 3 cut(s) 450, 998, 1072
AcyI GRCGYC 1 cut(s) 1156
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 9 cut(s) 314, 319, 617, 653, 692, 906, 1024, 1114, 1133
AhdI GACNNNNNGTC 1 cut(s) 1155
AluBI AGCT 8 cut(s) 135, 194, 287, 310, 492, 662, 830, 1187
AluI AGCT 8 cut(s) 135, 194, 287, 310, 492, 662, 830, 1187
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 756
Alw44I GTGCAC 1 cut(s) 501
AlwI GGATC 2 cut(s) 779, 887
Ama87I CYCGRG 1 cut(s) 1078
AoxI GGCC 2 cut(s) 87, 1075
ApaLI GTGCAC 1 cut(s) 501
ApoI RAATTY 3 cut(s) 271, 314, 885
AspLEI GCGC 3 cut(s) 127, 698, 821
AspS9I GGNCC 2 cut(s) 88, 1076
AsuC2I CCSGG 3 cut(s) 1034, 1079, 1080
AsuHPI GGTGA 3 cut(s) 245, 271, 1081
AvaI CYCGRG 1 cut(s) 1078
BaeGI GKGCMC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 505
BbvCI CCTCAGC 2 cut(s) 190, 744
BccI CCATC 2 cut(s) 99, 809
BceAI ACGGC 1 cut(s) 112
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 3 cut(s) 1034, 1079, 1080
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 4 cut(s) 600, 825, 897, 1144
BfoI RGCGCY 2 cut(s) 699, 822
BfuI GTATCC 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 1034, 1079, 1080
BmeRI GACNNNNNGTC 1 cut(s) 1155
BmeT110I CYCGRG 1 cut(s) 1078
BmgT120I GGNCC 2 cut(s) 88, 1076
BmrFI CCNGG 3 cut(s) 1034, 1079, 1080
BmsI GCATC 4 cut(s) 274, 499, 860, 1195
BpmI CTGGAG 1 cut(s) 394
Bpu10I CCTNAGC 2 cut(s) 190, 744
BpuEI CTTGAG 2 cut(s) 368, 478
BpuMI CCSGG 3 cut(s) 1034, 1079, 1080
BsaHI GRCGYC 1 cut(s) 1156
BsaJI CCNNGG 2 cut(s) 534, 1078
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 515, 545, 1110, 1140
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 1156
Bse3DI GCAATG 1 cut(s) 990
BseDI CCNNGG 2 cut(s) 534, 1078
BseGI GGATG 3 cut(s) 820, 994, 1207
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 5 cut(s) 182, 204, 735, 801, 1092
BseNI ACTGG 1 cut(s) 1156
BseRI GAGGAG 7 cut(s) 14, 52, 103, 127, 130, 178, 190
BseSI GKGCMC 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 89, 1077
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 1078
BsiSI CCGG 2 cut(s) 1034, 1079
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 89, 1077
BsoBI CYCGRG 1 cut(s) 1078
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 3 cut(s) 103, 784, 892
BspACI CCGC 6 cut(s) 37, 46, 49, 109, 178, 848
BspANI GGCC 2 cut(s) 89, 1077
BspCNI CTCAG 5 cut(s) 181, 203, 736, 802, 1093
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 2 cut(s) 781, 804
BspPI GGATC 2 cut(s) 779, 887
BspQI GCTCTTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 990
BsrI ACTGG 1 cut(s) 1156
BssECI CCNNGG 2 cut(s) 534, 1078
BssMI GATC 3 cut(s) 103, 784, 892
BssNI GRCGYC 1 cut(s) 1156
BssT1I CCWWGG 1 cut(s) 534
Bst4CI ACNGT 4 cut(s) 210, 332, 394, 1176
Bst6I CTCTTC 2 cut(s) 188, 708
BstACI GRCGYC 1 cut(s) 1156
BstAPI GCANNNNNTGC 2 cut(s) 473, 509
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 5 cut(s) 168, 190, 744, 810, 1101
BstF5I GGATG 3 cut(s) 820, 994, 1207
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 2 cut(s) 699, 822
BstHHI GCGC 3 cut(s) 127, 698, 821
BstKTI GATC 3 cut(s) 106, 787, 895
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 3 cut(s) 103, 784, 892
BstMCI CGRYCG 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 968
BstSCI CCNGG 3 cut(s) 1032, 1077, 1078
BstSFI CTRYAG 4 cut(s) 600, 825, 897, 1144
BstSLI GKGCMC 1 cut(s) 505
BstUI CGCG 1 cut(s) 125
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 2 cut(s) 89, 1077
BtsCI GGATG 3 cut(s) 820, 994, 1207
Cac8I GCNNGC 1 cut(s) 501
CciI TCATGA 2 cut(s) 781, 804
CfoI GCGC 3 cut(s) 127, 698, 821
Cfr13I GGNCC 2 cut(s) 88, 1076
Cfr9I CCCGGG 1 cut(s) 1078
CseI GACGC 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 605, 640
CviAII CATG 6 cut(s) 416, 608, 751, 782, 805, 965
DdeI CTNAG 5 cut(s) 168, 190, 744, 810, 1101
DpnI GATC 3 cut(s) 105, 786, 894
DpnII GATC 3 cut(s) 103, 784, 892
DriI GACNNNNNGTC 1 cut(s) 1155
Eam1104I CTCTTC 2 cut(s) 188, 708
Eam1105I GACNNNNNGTC 1 cut(s) 1155
EarI CTCTTC 2 cut(s) 188, 708
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 534
Eco32I GATATC 1 cut(s) 561
Eco57I CTGAAG 3 cut(s) 450, 998, 1072
Eco88I CYCGRG 1 cut(s) 1078
EcoRI GAATTC 1 cut(s) 314
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 1245
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 6 cut(s) 419, 611, 754, 785, 808, 968
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 6 cut(s) 415, 607, 750, 781, 804, 964
FauI CCCGC 1 cut(s) 53
FokI GGATG 3 cut(s) 827, 1001, 1214
FspBI CTAG 1 cut(s) 371
GlaI GCGC 3 cut(s) 126, 697, 820
GsuI CTGGAG 1 cut(s) 394
HaeII RGCGCY 2 cut(s) 699, 822
HaeIII GGCC 2 cut(s) 89, 1077
HapII CCGG 2 cut(s) 1034, 1079
HgaI GACGC 1 cut(s) 251
HhaI GCGC 3 cut(s) 127, 698, 821
Hin1I GRCGYC 1 cut(s) 1156
Hin1II CATG 6 cut(s) 419, 611, 754, 785, 808, 968
Hin6I GCGC 3 cut(s) 125, 696, 819
HinP1I GCGC 3 cut(s) 125, 696, 819
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 4 cut(s) 224, 958, 1043, 1083
HpaII CCGG 2 cut(s) 1034, 1079
HphI GGTGA 3 cut(s) 245, 271, 1081
Hpy166II GTNNAC 2 cut(s) 390, 503
Hpy188I TCNGA 2 cut(s) 948, 1091
Hpy188III TCNNGA 6 cut(s) 727, 782, 805, 1021, 1160, 1181
Hpy8I GTNNAC 2 cut(s) 390, 503
Hpy99I CGWCG 4 cut(s) 13, 16, 104, 245
HpyAV CCTTC 4 cut(s) 243, 686, 1000, 1192
HpyCH4III ACNGT 4 cut(s) 210, 332, 394, 1176
HpyCH4IV ACGT 2 cut(s) 8, 1156
HpyF3I CTNAG 5 cut(s) 168, 190, 744, 810, 1101
HpySE526I ACGT 2 cut(s) 8, 1156
Hsp92I GRCGYC 1 cut(s) 1156
Hsp92II CATG 6 cut(s) 419, 611, 754, 785, 808, 968
HspAI GCGC 3 cut(s) 125, 696, 819
Kzo9I GATC 3 cut(s) 103, 784, 892
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 2 cut(s) 116, 140
LweI GCATC 4 cut(s) 274, 499, 860, 1195
MaeI CTAG 1 cut(s) 371
MaeII ACGT 2 cut(s) 8, 1156
MaeIII GTNAC 2 cut(s) 343, 871
MalI GATC 3 cut(s) 105, 786, 894
MbiI CCGCTC 1 cut(s) 111
MboI GATC 3 cut(s) 103, 784, 892
MboII GAAGA 5 cut(s) 175, 268, 695, 1004, 1078
MfeI CAATTG 1 cut(s) 906
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 8 cut(s) 271, 314, 755, 885, 906, 972, 1135, 1231
MlyI GAGTC 1 cut(s) 218
Mph1103I ATGCAT 1 cut(s) 1245
MseI TTAA 5 cut(s) 249, 297, 758, 971, 1095
MslI CAYNNNNRTG 1 cut(s) 358
MspA1I CMGCKG 3 cut(s) 51, 178, 194
MspI CCGG 2 cut(s) 1034, 1079
MspR9I CCNGG 3 cut(s) 1034, 1079, 1080
MunI CAATTG 1 cut(s) 906
Mva1269I GAATGC 1 cut(s) 439
MvnI CGCG 1 cut(s) 125
NciI CCSGG 3 cut(s) 1034, 1079, 1080
NdeII GATC 3 cut(s) 103, 784, 892
NlaIII CATG 6 cut(s) 419, 611, 754, 785, 808, 968
NmuCI GTSAC 2 cut(s) 343, 871
NsiI ATGCAT 1 cut(s) 1245
NspI RCATGY 1 cut(s) 968
PagI TCATGA 2 cut(s) 781, 804
PciSI GCTCTTC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 3 cut(s) 958, 1043, 1083
Ple19I CGATCG 1 cut(s) 106
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PspPI GGNCC 2 cut(s) 88, 1076
PsrI GAACNNNNNNTAC 2 cut(s) 993, 1025
PvuI CGATCG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 5 cut(s) 249, 297, 758, 971, 1095
Sau3AI GATC 3 cut(s) 103, 784, 892
Sau96I GGNCC 2 cut(s) 88, 1076
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 3 cut(s) 1034, 1079, 1080
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 4 cut(s) 274, 499, 860, 1195
SfcI CTRYAG 4 cut(s) 600, 825, 897, 1144
SmaI CCCGGG 1 cut(s) 1080
SmiMI CAYNNNNRTG 1 cut(s) 358
SmlI CTYRAG 2 cut(s) 383, 493
SmoI CTYRAG 2 cut(s) 383, 493
Sse9I AATT 8 cut(s) 271, 314, 755, 885, 906, 972, 1135, 1231
SsiI CCGC 6 cut(s) 37, 46, 49, 109, 178, 848
SspI AATATT 1 cut(s) 649
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 3 cut(s) 1032, 1077, 1078
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 4 cut(s) 210, 332, 394, 1176
TaiI ACGT 2 cut(s) 11, 1159
TaqI TCGA 3 cut(s) 222, 268, 961
TasI AATT 8 cut(s) 271, 314, 755, 885, 906, 972, 1135, 1231
TauI GCSGC 4 cut(s) 51, 111, 181, 851
TfiI GAWTC 3 cut(s) 958, 1043, 1083
Tru1I TTAA 5 cut(s) 249, 297, 758, 971, 1095
Tru9I TTAA 5 cut(s) 249, 297, 758, 971, 1095
TseFI GTSAC 2 cut(s) 343, 871
Tsp45I GTSAC 2 cut(s) 343, 871
TspDTI ATGAA 2 cut(s) 596, 767
TspGWI ACGGA 2 cut(s) 433, 540
TspMI CCCGGG 1 cut(s) 1078
VneI GTGCAC 1 cut(s) 501
XapI RAATTY 3 cut(s) 271, 314, 885
XceI RCATGY 1 cut(s) 968
XmaI CCCGGG 1 cut(s) 1078
XspI CTAG 1 cut(s) 371
ZraI GACGTC 1 cut(s) 1157
Zsp2I ATGCAT 1 cut(s) 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.