Rmu_sc0003767.1_g000027

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003767.1
OG0000942
Physical Location & Seq
Reverse (-)
110835 .. 113696
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003767.1_g000027.1.cds

Sequence Viewer

Length: 1002 bp
atggactcgtcatcttcatcttcatcttttacctctgcaaccttcgctctcaacttagccatggcgcctccctcatggttgtttccacctttaacatccacaaacgcagcgtcgaccaagtcaacggctggctcatccagatccgcctcaatctgccgtctcgcgttaccgccgaagaggacgccgacgaagaagattacatcaaaaaagatgatcaggagtggccgcgagggtttgggttcggactgcgagaccgttattgatcagaaaacaacgccgtccaggtccttggacgagaaatcaattccctcttacagaatctcatcctcgctgcccaattcgaatatgagaagaggcggtttcatcgaggaggccaagtttgatccactctcgaattatcgagctcctagtttttcttcttcagtggacaagctcaatttcaccgcctccctccactccattggcatagtcgactcagattgctcgcctccagtccagggtcaagcaccgcctccctccagtccatccttccctcttctctgtgccggcaggcttgggctaagttctgaacatgagttgtggcgcagtcggcgagcgaagatcttaatcttggtgtggatgagagctatactctggtgtcttggttccggtgcaactatagttcatggagttcaaaacttgtgggtttcacagccccctccaaatattcaatatgcaggtatagtgattggtggctcgtttattattgaaggcgcttctcttcttgttgccatacaagctgtcaagaaaggtgttgctgctgagggcatgaaattaagagactatatatggcgtggtcatgatcctacctctgttgcagtcatgactgaggatggcactgctgtagctggtcttgctattgctgcaccatcattggttgctgtaaatgtcacaggaaatggaatttacgatcctataggttcatttgtagttggcaaggatcttggaatggtggctatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

333

Amino Acids

35.16

Weight (kDa)

9.44

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 707
AccB1I GGYRCC 1 cut(s) 64
AccI GTMKAC 2 cut(s) 113, 471
AccII CGCG 2 cut(s) 164, 228
AciI CCGC 6 cut(s) 144, 170, 226, 357, 444, 509
AclWI GGATC 5 cut(s) 135, 377, 836, 944, 987
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 942
AcuI CTGAAG 1 cut(s) 405
AcyI GRCGYC 2 cut(s) 65, 182
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 3 cut(s) 674, 710, 749
AjnI CCWGG 2 cut(s) 281, 495
AluBI AGCT 5 cut(s) 404, 433, 626, 779, 887
AluI AGCT 5 cut(s) 404, 433, 626, 779, 887
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 3 cut(s) 164, 245, 813
AlwI GGATC 5 cut(s) 135, 377, 836, 944, 987
AoxI GGCC 2 cut(s) 223, 372
ApeKI GCWGC 4 cut(s) 107, 331, 797, 902
ApoI RAATTY 1 cut(s) 942
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspLEI GCGC 3 cut(s) 67, 585, 755
AspS9I GGNCC 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 433
AsuII TTCGAA 1 cut(s) 341
AvaII GGWCC 1 cut(s) 285
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 406
BbvCI CCTCAGC 1 cut(s) 801
BbvI GCAGC 4 cut(s) 119, 318, 784, 889
BccI CCATC 3 cut(s) 532, 866, 916
BceAI ACGGC 3 cut(s) 141, 141, 262
BciT130I CCWGG 2 cut(s) 283, 497
BclI TGATCA 2 cut(s) 213, 262
BcoDI GTCTC 3 cut(s) 164, 245, 813
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 3 cut(s) 657, 882, 954
BfoI RGCGCY 2 cut(s) 68, 756
BfuAI ACCTGC 1 cut(s) 707
BglII AGATCT 1 cut(s) 600
BisI GCNGC 5 cut(s) 108, 226, 332, 798, 903
BlsI GCNGC 5 cut(s) 109, 227, 333, 799, 904
Bme1390I CCNGG 2 cut(s) 283, 497
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 2 cut(s) 66, 646
BmrFI CCNGG 2 cut(s) 283, 497
BplI GAGNNNNNCTC 2 cut(s) 615, 647
BpmI CTGGAG 2 cut(s) 474, 502
Bpu10I CCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 605
BsaHI GRCGYC 2 cut(s) 65, 182
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 60, 288, 496
BsaWI WCCGGW 1 cut(s) 647
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 2 cut(s) 491, 519
Bse8I GATNNNNATC 1 cut(s) 605
BseBI CCWGG 2 cut(s) 283, 497
BseDI CCNNGG 3 cut(s) 60, 288, 496
BseGI GGATG 6 cut(s) 95, 134, 323, 524, 624, 877
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 3 cut(s) 489, 792, 858
BseNI ACTGG 2 cut(s) 491, 519
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 4 cut(s) 119, 318, 784, 889
BsgI GTGCAG 1 cut(s) 888
Bsh1236I CGCG 2 cut(s) 164, 228
BshFI GGCC 2 cut(s) 225, 374
BshNI GGYRCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 2 cut(s) 546, 648
BslI CCNNNNNNNGG 1 cut(s) 497
BsmAI GTCTC 3 cut(s) 164, 245, 813
BsmBI CGTCTC 1 cut(s) 164
BsnI GGCC 2 cut(s) 225, 374
Bso31I GGTCTC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 6 cut(s) 144, 170, 226, 357, 444, 509
BspANI GGCC 2 cut(s) 225, 374
BspCNI CTCAG 3 cut(s) 488, 793, 859
BspFNI CGCG 2 cut(s) 164, 228
BspHI TCATGA 2 cut(s) 838, 861
BspLI GGNNCC 2 cut(s) 66, 646
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 5 cut(s) 135, 377, 836, 944, 987
BspT104I TTCGAA 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 2 cut(s) 491, 519
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 3 cut(s) 60, 288, 496
BssMI GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
BssNI GRCGYC 2 cut(s) 65, 182
BssT1I CCWWGG 2 cut(s) 60, 288
Bst2UI CCWGG 2 cut(s) 283, 497
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 4 cut(s) 170, 346, 540, 765
BstACI GRCGYC 2 cut(s) 65, 182
BstBI TTCGAA 1 cut(s) 341
BstC8I GCNNGC 5 cut(s) 130, 485, 547, 551, 594
BstDEI CTNAG 5 cut(s) 55, 475, 560, 801, 867
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 6 cut(s) 95, 134, 323, 524, 624, 877
BstFNI CGCG 2 cut(s) 164, 228
BstH2I RGCGCY 2 cut(s) 68, 756
BstHHI GCGC 3 cut(s) 67, 585, 755
BstKTI GATC 8 cut(s) 143, 216, 265, 385, 603, 844, 952, 982
BstMAI GTCTC 3 cut(s) 164, 245, 813
BstMBI GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
BstMWI GCNNNNNNNGC 5 cut(s) 44, 589, 776, 893, 902
BstNI CCWGG 2 cut(s) 283, 497
BstSCI CCNGG 2 cut(s) 281, 495
BstSFI CTRYAG 3 cut(s) 657, 882, 954
BstUI CGCG 2 cut(s) 164, 228
BstV1I GCAGC 4 cut(s) 119, 318, 784, 889
BstX2I RGATCY 3 cut(s) 140, 600, 979
BstXI CCANNNNNNTGG 2 cut(s) 289, 461
BstYI RGATCY 3 cut(s) 140, 600, 979
BsuRI GGCC 2 cut(s) 225, 374
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 6 cut(s) 95, 134, 323, 524, 624, 877
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 2 cut(s) 429, 876
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 5 cut(s) 130, 485, 547, 551, 594
CciI TCATGA 2 cut(s) 838, 861
CfoI GCGC 3 cut(s) 67, 585, 755
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 285
CseI GACGC 2 cut(s) 99, 190
CspCI CAANNNNNGTGG 2 cut(s) 662, 697
CviAII CATG 7 cut(s) 61, 75, 572, 665, 808, 839, 862
DdeI CTNAG 5 cut(s) 55, 475, 560, 801, 867
DinI GGCGCC 1 cut(s) 66
DpnI GATC 8 cut(s) 142, 215, 264, 384, 602, 843, 951, 981
DpnII GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 4 cut(s) 170, 346, 540, 765
EarI CTCTTC 4 cut(s) 170, 346, 540, 765
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 404
Eco130I CCWWGG 2 cut(s) 60, 288
Eco24I GRGCYC 1 cut(s) 406
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 405
EcoICRI GAGCTC 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 281, 495
EcoT14I CCWWGG 2 cut(s) 60, 288
EcoT38I GRGCYC 1 cut(s) 406
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
ErhI CCWWGG 2 cut(s) 60, 288
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 7 cut(s) 64, 78, 575, 668, 811, 842, 865
FatI CATG 7 cut(s) 60, 74, 571, 664, 807, 838, 861
FbaI TGATCA 2 cut(s) 213, 262
FblI GTMKAC 2 cut(s) 113, 471
Fnu4HI GCNGC 5 cut(s) 108, 226, 332, 798, 903
FokI GGATG 6 cut(s) 82, 121, 310, 511, 631, 884
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 5 cut(s) 108, 226, 332, 798, 903
FspBI CTAG 1 cut(s) 408
GlaI GCGC 3 cut(s) 66, 584, 754
GluI GCNGC 5 cut(s) 108, 226, 332, 798, 903
GsuI CTGGAG 2 cut(s) 474, 502
HaeII RGCGCY 2 cut(s) 68, 756
HaeIII GGCC 2 cut(s) 225, 374
HapII CCGG 2 cut(s) 546, 648
HgaI GACGC 2 cut(s) 99, 190
HhaI GCGC 3 cut(s) 67, 585, 755
Hin1I GRCGYC 2 cut(s) 65, 182
Hin1II CATG 7 cut(s) 64, 78, 575, 668, 811, 842, 865
Hin6I GCGC 3 cut(s) 65, 583, 753
HinP1I GCGC 3 cut(s) 65, 583, 753
HincII GTYRAC 3 cut(s) 114, 123, 472
HindII GTYRAC 3 cut(s) 114, 123, 472
HinfI GANTC 3 cut(s) 5, 318, 473
HpaII CCGG 2 cut(s) 546, 648
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 4 cut(s) 114, 123, 427, 472
Hpy188I TCNGA 5 cut(s) 244, 267, 478, 568, 1001
Hpy188III TCNNGA 6 cut(s) 138, 217, 391, 784, 839, 862
Hpy8I GTNNAC 4 cut(s) 114, 123, 427, 472
Hpy99I CGWCG 2 cut(s) 115, 190
HpyAV CCTTC 3 cut(s) 52, 538, 743
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4V TGCA 5 cut(s) 38, 653, 716, 857, 905
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 589, 776, 893, 902
HpyF3I CTNAG 5 cut(s) 55, 475, 560, 801, 867
Hsp92I GRCGYC 2 cut(s) 65, 182
Hsp92II CATG 7 cut(s) 64, 78, 575, 668, 811, 842, 865
HspAI GCGC 3 cut(s) 65, 583, 753
KasI GGCGCC 1 cut(s) 64
KroI GCCGGC 1 cut(s) 545
KroNI GCCGGC 1 cut(s) 547
Ksp22I TGATCA 2 cut(s) 213, 262
Kzo9I GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 4 cut(s) 119, 318, 784, 889
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 165, 928
MalI GATC 8 cut(s) 142, 215, 264, 384, 602, 843, 951, 981
MboI GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
MflI RGATCY 3 cut(s) 140, 600, 979
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 6 cut(s) 303, 337, 394, 436, 812, 942
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 1 cut(s) 467
MroNI GCCGGC 1 cut(s) 545
MseI TTAA 3 cut(s) 92, 605, 815
MspI CCGG 2 cut(s) 546, 648
MspR9I CCNGG 2 cut(s) 283, 497
MvaI CCWGG 2 cut(s) 283, 497
MvnI CGCG 2 cut(s) 164, 228
MwoI GCNNNNNNNGC 5 cut(s) 44, 589, 776, 893, 902
NaeI GCCGGC 1 cut(s) 547
NarI GGCGCC 1 cut(s) 65
NcoI CCATGG 1 cut(s) 60
NdeII GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
NgoMIV GCCGGC 1 cut(s) 545
NlaIII CATG 7 cut(s) 64, 78, 575, 668, 811, 842, 865
NlaIV GGNNCC 2 cut(s) 66, 646
NmuCI GTSAC 1 cut(s) 928
NspV TTCGAA 1 cut(s) 341
PagI TCATGA 2 cut(s) 838, 861
PdiI GCCGGC 1 cut(s) 547
PfeI GAWTC 1 cut(s) 318
PflFI GACNNNGTC 1 cut(s) 118
PkrI GCNGC 5 cut(s) 109, 227, 333, 799, 904
PleI GAGTC 1 cut(s) 467
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 406
Psp5II RGGWCCY 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 281, 495
PspGI CCWGG 2 cut(s) 281, 495
PspN4I GGNNCC 2 cut(s) 66, 646
PspPI GGNCC 1 cut(s) 285
PspPPI RGGWCCY 1 cut(s) 285
PsuI RGATCY 3 cut(s) 140, 600, 979
PsyI GACNNNGTC 1 cut(s) 118
SacI GAGCTC 1 cut(s) 406
SalI GTCGAC 2 cut(s) 112, 470
SaqAI TTAA 3 cut(s) 92, 605, 815
SatI GCNGC 5 cut(s) 108, 226, 332, 798, 903
Sau3AI GATC 8 cut(s) 140, 213, 262, 382, 600, 841, 949, 979
Sau96I GGNCC 1 cut(s) 285
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 2 cut(s) 283, 497
SduI GDGCHC 1 cut(s) 406
SfcI CTRYAG 3 cut(s) 657, 882, 954
SfoI GGCGCC 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 341
SinI GGWCC 1 cut(s) 285
Sse9I AATT 6 cut(s) 303, 337, 394, 436, 812, 942
SsiI CCGC 6 cut(s) 144, 170, 226, 357, 444, 509
SspDI GGCGCC 1 cut(s) 64
SspI AATATT 1 cut(s) 706
SspMI CTAG 1 cut(s) 408
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 281, 495
StyI CCWWGG 2 cut(s) 60, 288
TaaI ACNGT 1 cut(s) 256
TaqI TCGA 6 cut(s) 113, 341, 366, 392, 400, 471
TasI AATT 6 cut(s) 303, 337, 394, 436, 812, 942
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 1 cut(s) 318
Tru1I TTAA 3 cut(s) 92, 605, 815
Tru9I TTAA 3 cut(s) 92, 605, 815
TscAI CASTG 2 cut(s) 429, 883
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 4 cut(s) 107, 331, 797, 902
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 6 cut(s) 6, 12, 352, 653, 824, 951
TspRI CASTG 2 cut(s) 429, 883
Tth111I GACNNNGTC 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 285
XapI RAATTY 1 cut(s) 942
XmiI GTMKAC 2 cut(s) 113, 471
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.