RchiOBHm_Chr2g0149771

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000942
Physical Location & Seq
Reverse (-)
67567402 .. 67572152
4751 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51916

Sequence Viewer

Length: 1365 bp
ATGAGAACGTCGTCGTTTTCTCTCCTCTCTCGCCTCCGCCACTCCCGCCGCTGCTGCTACACCTCCTCTAACTCTCTGCTTTTCCACTTGGCCCATCAAAACGACGATCGCCGCTCCTCCCAAAACGCGCCGTTTAGCTCCTCCTCCTTTCACTCCTACTCCACTCACACTCAGTTGCAGCGGCTCTTCTCCTCAGCTGCCTCCTCCAAACGGTTCGTTTTGCTCGACTTGCTTCCTTCCCCCGACGCTGTTAATCACCACTCGTTGCATCGAAGTTTCTTCACCAGAGCTAAACCTGTTAAACAAGTCAATCTTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTAGTGTTTTCGCTCAAGTTCACTGTCTGGTTATCCACCTCCAGCCATGTAATGTTGGCTGAAGTTGTGCATTCCGTTGCTGATTTTGCTAATCAGGCACTTCTTGCTTATGGGTTGAGTAGCTCAAGGCGTGCACCAGATGCTCTCCACCCTTACGGATACTCCAAGGAAAGATTTGTTTGGTCTTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCCGGTGCAACTATAGTTCATGGAGTTCAAAACTTGTGGGTTTCACAGCCCCCTCCAAATATTCAATATGCAGCTTTAGTGATTGGTGGTTCGTTTATTATTGAAGGCGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGGTGCTGCTGCTGAGGGCATGAAATTAAGAGACTATATATGGCGTGGTCATGATCCTACCTCTGTTGCAGTCATGACTGAGGATGGCGCTGCTGTAGCTGGTCTTGCTATTGCTGCAGCATCATTGGTTGCTGTAAATGTCACAGGAAATGGAATTTACGATCCTATAGGTTCAATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATCTTTCTGATACAAAGGAATCGACATGCTTTAATTGGTAGGGCAATGGATGACTATGATGTAGAGAAGGTTCTTCAGTTCTTGAAAAATGACCCGGTTGTAGATTCTTTATATGATTGCAAAAGCGAGGTGATTGGCCCGGGATTCTTCAGATTTAAGGCTGAGATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTACCTCTATAGGACTGGACGTCAAGAATGGGCAAGACAGTTTCGTGAAGCTGCAAAGGAGAAGGATGATGCTGCTTTGCTGAAAATGATGTCCAATTATGGTGAAGAAGTAGTTACAGCCTTGGGAAGTGAGGTTGATAGATTGGAAAAGGAGATTCAAGAGCTTGTTCCTGGTATACGGCATGTCGACATTGAGGCACACAATCCAATAGAGCTGTCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

454

Amino Acids

50.16

Weight (kDa)

7.76

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 117 - 325 1.2e-30 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1162
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 2 cut(s) 1315, 1326
AccII CGCG 1 cut(s) 128
AciI CCGC 5 cut(s) 37, 46, 49, 112, 181
AclWI GGATC 2 cut(s) 782, 890
AcsI RAATTY 2 cut(s) 317, 888
AcuI CTGAAG 3 cut(s) 453, 1001, 1075
AcvI CACGTG 1 cut(s) 1362
AcyI GRCGYC 1 cut(s) 1159
AfiI CCNNNNNNNGG 1 cut(s) 210
AhdI GACNNNNNGTC 1 cut(s) 1158
AjnI CCWGG 1 cut(s) 1309
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 1 cut(s) 759
Alw44I GTGCAC 1 cut(s) 504
AlwI GGATC 2 cut(s) 782, 890
Ama87I CYCGRG 1 cut(s) 1081
AoxI GGCC 2 cut(s) 90, 1078
ApaLI GTGCAC 1 cut(s) 504
ApoI RAATTY 2 cut(s) 317, 888
AspLEI GCGC 3 cut(s) 130, 701, 824
AspS9I GGNCC 2 cut(s) 91, 1079
AsuC2I CCSGG 3 cut(s) 1037, 1082, 1083
AsuHPI GGTGA 4 cut(s) 248, 274, 1084, 1253
AvaI CYCGRG 1 cut(s) 1081
BaeGI GKGCMC 1 cut(s) 508
BbrPI CACGTG 1 cut(s) 1362
Bbv12I GWGCWC 1 cut(s) 508
BbvCI CCTCAGC 2 cut(s) 193, 747
BccI CCATC 2 cut(s) 102, 812
BceAI ACGGC 2 cut(s) 115, 1334
BciT130I CCWGG 1 cut(s) 1311
BciVI GTATCC 1 cut(s) 524
BcnI CCSGG 3 cut(s) 1037, 1082, 1083
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 5 cut(s) 603, 828, 849, 900, 1147
BfoI RGCGCY 2 cut(s) 702, 825
BfuI GTATCC 1 cut(s) 524
Bme1390I CCNGG 4 cut(s) 1037, 1082, 1083, 1311
BmeRI GACNNNNNGTC 1 cut(s) 1158
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 2 cut(s) 91, 1079
BmiI GGNNCC 1 cut(s) 592
BmrFI CCNGG 4 cut(s) 1037, 1082, 1083, 1311
BmsI GCATC 4 cut(s) 277, 502, 863, 1198
BpmI CTGGAG 1 cut(s) 397
Bpu10I CCTNAGC 2 cut(s) 193, 747
BpuEI CTTGAG 2 cut(s) 371, 481
BpuMI CCSGG 3 cut(s) 1037, 1082, 1083
BsaAI YACGTR 1 cut(s) 1362
BsaHI GRCGYC 1 cut(s) 1159
BsaJI CCNNGG 3 cut(s) 537, 1081, 1260
BsaWI WCCGGW 1 cut(s) 593
BsaXI ACNNNNNCTCC 6 cut(s) 143, 173, 518, 548, 1113, 1143
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 1159
Bse3DI GCAATG 1 cut(s) 993
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 3 cut(s) 537, 1081, 1260
BseGI GGATG 3 cut(s) 823, 997, 1210
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMI GCAATG 1 cut(s) 993
BseMII CTCAG 5 cut(s) 185, 207, 738, 804, 1095
BseNI ACTGG 1 cut(s) 1159
BseRI GAGGAG 7 cut(s) 14, 55, 106, 130, 133, 181, 193
BseSI GKGCMC 1 cut(s) 508
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 92, 1080
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 508
BsiHKCI CYCGRG 1 cut(s) 1081
BsiSI CCGG 3 cut(s) 594, 1037, 1082
BslI CCNNNNNNNGG 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 2 cut(s) 92, 1080
BsoBI CYCGRG 1 cut(s) 1081
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 3 cut(s) 106, 787, 895
BspACI CCGC 5 cut(s) 37, 46, 49, 112, 181
BspANI GGCC 2 cut(s) 92, 1080
BspCNI CTCAG 5 cut(s) 184, 206, 739, 805, 1096
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 2 cut(s) 784, 807
BspLI GGNNCC 1 cut(s) 592
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 2 cut(s) 782, 890
BspQI GCTCTTC 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 993
BsrI ACTGG 1 cut(s) 1159
BssECI CCNNGG 3 cut(s) 537, 1081, 1260
BssMI GATC 3 cut(s) 106, 787, 895
BssNAI GTATAC 1 cut(s) 1316
BssNI GRCGYC 1 cut(s) 1159
BssT1I CCWWGG 2 cut(s) 537, 1260
Bst1107I GTATAC 1 cut(s) 1316
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 4 cut(s) 213, 335, 397, 1179
Bst6I CTCTTC 2 cut(s) 191, 711
BstACI GRCGYC 1 cut(s) 1159
BstAPI GCANNNNNTGC 2 cut(s) 476, 512
BstBAI YACGTR 1 cut(s) 1362
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 5 cut(s) 171, 193, 747, 813, 1104
BstF5I GGATG 3 cut(s) 823, 997, 1210
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 2 cut(s) 702, 825
BstHHI GCGC 3 cut(s) 130, 701, 824
BstKTI GATC 3 cut(s) 109, 790, 898
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 3 cut(s) 106, 787, 895
BstMCI CGRYCG 1 cut(s) 109
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 2 cut(s) 971, 1325
BstSCI CCNGG 4 cut(s) 1035, 1080, 1081, 1309
BstSFI CTRYAG 5 cut(s) 603, 828, 849, 900, 1147
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 1 cut(s) 128
BstZ17I GTATAC 1 cut(s) 1316
BsuI GTATCC 1 cut(s) 524
BsuRI GGCC 2 cut(s) 92, 1080
BtsCI GGATG 3 cut(s) 823, 997, 1210
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 504
CciI TCATGA 2 cut(s) 784, 807
CfoI GCGC 3 cut(s) 130, 701, 824
Cfr13I GGNCC 2 cut(s) 91, 1079
Cfr9I CCCGGG 1 cut(s) 1081
CseI GACGC 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 608, 643
CviAII CATG 7 cut(s) 419, 611, 754, 785, 808, 968, 1322
DdeI CTNAG 5 cut(s) 171, 193, 747, 813, 1104
DpnI GATC 3 cut(s) 108, 789, 897
DpnII GATC 3 cut(s) 106, 787, 895
DriI GACNNNNNGTC 1 cut(s) 1158
Eam1104I CTCTTC 2 cut(s) 191, 711
Eam1105I GACNNNNNGTC 1 cut(s) 1158
EarI CTCTTC 2 cut(s) 191, 711
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 537, 1260
Eco32I GATATC 1 cut(s) 564
Eco57I CTGAAG 3 cut(s) 453, 1001, 1075
Eco72I CACGTG 1 cut(s) 1362
Eco88I CYCGRG 1 cut(s) 1081
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 1309
EcoRV GATATC 1 cut(s) 564
EcoT14I CCWWGG 2 cut(s) 537, 1260
ErhI CCWWGG 2 cut(s) 537, 1260
FaeI CATG 7 cut(s) 422, 614, 757, 788, 811, 971, 1325
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FatI CATG 7 cut(s) 418, 610, 753, 784, 807, 967, 1321
FauI CCCGC 1 cut(s) 53
FblI GTMKAC 2 cut(s) 1315, 1326
FokI GGATG 3 cut(s) 830, 1004, 1217
FspBI CTAG 1 cut(s) 374
GlaI GCGC 3 cut(s) 129, 700, 823
GsuI CTGGAG 1 cut(s) 397
HaeII RGCGCY 2 cut(s) 702, 825
HaeIII GGCC 2 cut(s) 92, 1080
HapII CCGG 3 cut(s) 594, 1037, 1082
HgaI GACGC 1 cut(s) 254
HhaI GCGC 3 cut(s) 130, 701, 824
Hin1I GRCGYC 1 cut(s) 1159
Hin1II CATG 7 cut(s) 422, 614, 757, 788, 811, 971, 1325
Hin6I GCGC 3 cut(s) 128, 699, 822
HinP1I GCGC 3 cut(s) 128, 699, 822
HincII GTYRAC 1 cut(s) 1327
HindII GTYRAC 1 cut(s) 1327
HinfI GANTC 4 cut(s) 961, 1046, 1086, 1294
HpaII CCGG 3 cut(s) 594, 1037, 1082
HphI GGTGA 4 cut(s) 248, 274, 1084, 1253
Hpy166II GTNNAC 5 cut(s) 393, 506, 1316, 1327, 1359
Hpy188I TCNGA 2 cut(s) 951, 1094
Hpy188III TCNNGA 8 cut(s) 314, 730, 785, 808, 1024, 1163, 1184, 1298
Hpy8I GTNNAC 5 cut(s) 393, 506, 1316, 1327, 1359
Hpy99I CGWCG 4 cut(s) 13, 16, 107, 248
HpyAV CCTTC 4 cut(s) 246, 689, 1003, 1195
HpyCH4III ACNGT 4 cut(s) 213, 335, 397, 1179
HpyCH4IV ACGT 3 cut(s) 8, 1159, 1361
HpyF3I CTNAG 5 cut(s) 171, 193, 747, 813, 1104
HpySE526I ACGT 3 cut(s) 8, 1159, 1361
Hsp92I GRCGYC 1 cut(s) 1159
Hsp92II CATG 7 cut(s) 422, 614, 757, 788, 811, 971, 1325
HspAI GCGC 3 cut(s) 128, 699, 822
Kzo9I GATC 3 cut(s) 106, 787, 895
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 2 cut(s) 119, 143
LweI GCATC 4 cut(s) 277, 502, 863, 1198
MaeI CTAG 1 cut(s) 374
MaeII ACGT 3 cut(s) 8, 1159, 1361
MaeIII GTNAC 3 cut(s) 346, 874, 1252
MalI GATC 3 cut(s) 108, 789, 897
MbiI CCGCTC 1 cut(s) 114
MboI GATC 3 cut(s) 106, 787, 895
MboII GAAGA 6 cut(s) 178, 271, 698, 1007, 1081, 1256
MfeI CAATTG 1 cut(s) 909
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 7 cut(s) 317, 758, 888, 909, 975, 1138, 1234
MseI TTAA 5 cut(s) 252, 300, 761, 974, 1098
MslI CAYNNNNRTG 1 cut(s) 361
MspA1I CMGCKG 3 cut(s) 51, 181, 197
MspI CCGG 3 cut(s) 594, 1037, 1082
MspR9I CCNGG 4 cut(s) 1037, 1082, 1083, 1311
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 1 cut(s) 1311
MvnI CGCG 1 cut(s) 128
NciI CCSGG 3 cut(s) 1037, 1082, 1083
NdeII GATC 3 cut(s) 106, 787, 895
NlaIII CATG 7 cut(s) 422, 614, 757, 788, 811, 971, 1325
NlaIV GGNNCC 1 cut(s) 592
NmuCI GTSAC 2 cut(s) 346, 874
NspI RCATGY 2 cut(s) 971, 1325
PagI TCATGA 2 cut(s) 784, 807
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 442
PfeI GAWTC 4 cut(s) 961, 1046, 1086, 1294
Ple19I CGATCG 1 cut(s) 109
PmaCI CACGTG 1 cut(s) 1362
PmlI CACGTG 1 cut(s) 1362
Ppu21I YACGTR 1 cut(s) 1362
Psp6I CCWGG 1 cut(s) 1309
PspCI CACGTG 1 cut(s) 1362
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 1 cut(s) 592
PspPI GGNCC 2 cut(s) 91, 1079
PsrI GAACNNNNNNTAC 2 cut(s) 996, 1028
PstI CTGCAG 1 cut(s) 853
PvuI CGATCG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 361
SalI GTCGAC 1 cut(s) 1325
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 5 cut(s) 252, 300, 761, 974, 1098
Sau3AI GATC 3 cut(s) 106, 787, 895
Sau96I GGNCC 2 cut(s) 91, 1079
ScrFI CCNGG 4 cut(s) 1037, 1082, 1083, 1311
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 4 cut(s) 277, 502, 863, 1198
SfcI CTRYAG 5 cut(s) 603, 828, 849, 900, 1147
SmaI CCCGGG 1 cut(s) 1083
SmiMI CAYNNNNRTG 1 cut(s) 361
SmlI CTYRAG 2 cut(s) 386, 496
SmoI CTYRAG 2 cut(s) 386, 496
Sse9I AATT 7 cut(s) 317, 758, 888, 909, 975, 1138, 1234
SsiI CCGC 5 cut(s) 37, 46, 49, 112, 181
SspI AATATT 1 cut(s) 652
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 4 cut(s) 1035, 1080, 1081, 1309
StyI CCWWGG 2 cut(s) 537, 1260
TaaI ACNGT 4 cut(s) 213, 335, 397, 1179
TaiI ACGT 3 cut(s) 11, 1162, 1364
TaqI TCGA 4 cut(s) 225, 271, 964, 1326
TasI AATT 7 cut(s) 317, 758, 888, 909, 975, 1138, 1234
TauI GCSGC 3 cut(s) 51, 114, 184
TfiI GAWTC 4 cut(s) 961, 1046, 1086, 1294
Tru1I TTAA 5 cut(s) 252, 300, 761, 974, 1098
Tru9I TTAA 5 cut(s) 252, 300, 761, 974, 1098
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 2 cut(s) 346, 874
Tsp45I GTSAC 2 cut(s) 346, 874
TspDTI ATGAA 2 cut(s) 599, 770
TspGWI ACGGA 2 cut(s) 436, 543
TspMI CCCGGG 1 cut(s) 1081
TspRI CASTG 1 cut(s) 400
VneI GTGCAC 1 cut(s) 504
XapI RAATTY 2 cut(s) 317, 888
XceI RCATGY 2 cut(s) 971, 1325
XmaI CCCGGG 1 cut(s) 1081
XmiI GTMKAC 2 cut(s) 1315, 1326
XspI CTAG 1 cut(s) 374
ZraI GACGTC 1 cut(s) 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.