pycom09g07920

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000942
Physical Location & Seq
Reverse (-)
6050484 .. 6053966
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g07920.3

Sequence Viewer

Length: 1353 bp
ATGAGAACGTCCTCCTACACTCTCGTCTCCCGCCTTCGCCGCACCCACTGCTTCAGTCCTCGCTGCTCCTCCCAATTTCTGCTCTTTCACTACTCAGACACCAAGAACGACGATCGCAGCGTCCCCCAAAACGCTCCGTTTAGCTCCTTCACTCACACTCTGCTGCGCGGCCTGGTTTCCTCGGCTCCTTGCTCGAAACGGGTCGTCTCGCCGGACTTGGTTTCTTCGGGTAGTGTTGCTCTTCGTCAACAGCAGCAGCAGCACTTGCTCCATCGCAGCTTCTTTACTAGAGCGAAGCAGGTGAAGATTGAATTCAATGACCAGCACAGTCAAAGGGCAGTCACAACTGCATTGTGGTGCAACTTCCTTGTATTTTCTCTCAAGTTTGGTGTCTGGTTATCGACCTCTAGCCATGTTATGTTTGCTGAAGTTGTGCACTCGGTTGCGGATTTTGCAAATCAGGCGCTTCTTGCTTATGGTTTGAGTAGCTCAAGGCGTGCACCGGATGCTCTCCACCCTTATGGATACTCCAAGGAAAGATTTGTCTGGTCCTTGATATCTGCTGTTGGTATCTTTTGTCTTGGTTCGGGTGCGACTATCGTTCATGGAGTTCAAAACTTGTGGACTTCACAGCCTCCTCCAAATATTCAGTATGCAGCTCTTGTGATTGGTGGCTCATTTATTATTGAAGGTGCTTCTCTTCTTGTTGCCATACAAGCTGTCAAGAAAGGTGCAGCTGCTGAGGGCATGAAATTGAGAGACTTTATATGGCGCGGACACGATCCCACATCTGTTGCAGTCATGACAGAGGATGGTGCAGCTGTAACTGGTCTTGCTATTGCTGCGGCATCACTGGTTGCAGTGAATAGAACAGGAAATGCAATTTATGATCGATCGATTGTAGTTGGCAACCTACTTGGAATGGTGGCTATTTTTCTGATCCAAAGGAATCGACATGCTTTGATTGGCAGGGCAATGGATGACCATGATATGGAGAAGGTTCTCCAGTTTTTGAAAAATGACCCGGTTGTGGATTCTCTATATGATTGCAAAAGTGAGGTGATTGGTCCTGGATTCTTCAGATTTAAGGCTGAAATAGATTTCAATGGAGTGGTGGTAGTTCAAAATTATCTCTCTAGGACTGGGCGTGAAGAATGGGCAAGACAGTTTCATGAAGCTGCAAAGGAGAAGGATGATACTGCATTGCTTAAAATGATGTCAAACTATGGTGAAGAGGTAGTGACAGCGTTAGGAAGTGAAGTTGATAGATTGGAGAAGGAGATCCAAGCACTTGTTCCTGGTATACGGCATGTTGATATCGAGGCACACAATCCAATAGAACTATCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux

Protein Analysis

451

Amino Acids

49.73

Weight (kDa)

8.46

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 289
Acc36I ACCTGC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 991
AccI GTMKAC 1 cut(s) 1303
AccII CGCG 2 cut(s) 168, 774
AciI CCGC 6 cut(s) 31, 40, 168, 446, 774, 845
AclWI GGATC 3 cut(s) 776, 934, 1276
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 3 cut(s) 37, 447, 1063
AfiI CCNNNNNNNGG 2 cut(s) 991, 1030
AgsI TTSAA 7 cut(s) 311, 316, 614, 689, 1015, 1105, 1124
AjnI CCWGG 3 cut(s) 171, 1069, 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 1278, 1310
AluBI AGCT 8 cut(s) 144, 279, 489, 659, 719, 737, 821, 1178
AluI AGCT 8 cut(s) 144, 279, 489, 659, 719, 737, 821, 1178
Alw21I GWGCWC 2 cut(s) 438, 502
Alw26I GTCTC 3 cut(s) 31, 211, 753
Alw44I GTGCAC 2 cut(s) 434, 498
AlwI GGATC 3 cut(s) 776, 934, 1276
AlwNI CAGNNNCTG 1 cut(s) 740
AoxI GGCC 1 cut(s) 169
ApaLI GTGCAC 2 cut(s) 434, 498
ApoI RAATTY 1 cut(s) 311
AspLEI GCGC 3 cut(s) 168, 466, 774
AspS9I GGNCC 2 cut(s) 549, 1067
AsuC2I CCSGG 1 cut(s) 1025
AsuHPI GGTGA 3 cut(s) 313, 1072, 1241
AvaII GGWCC 2 cut(s) 549, 1067
BaeGI GKGCMC 2 cut(s) 438, 502
Bbv12I GWGCWC 2 cut(s) 438, 502
BbvCI CCTCAGC 1 cut(s) 741
BccI CCATC 2 cut(s) 279, 806
BceAI ACGGC 1 cut(s) 1322
BciT130I CCWGG 3 cut(s) 173, 1071, 1299
BciVI GTATCC 1 cut(s) 518
BcnI CCSGG 1 cut(s) 1025
BcoDI GTCTC 3 cut(s) 31, 211, 753
BfaI CTAG 3 cut(s) 288, 408, 1137
BfoI RGCGCY 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 289
BfuI GTATCC 1 cut(s) 518
Bme1390I CCNGG 4 cut(s) 173, 1025, 1071, 1299
Bme18I GGWCC 2 cut(s) 549, 1067
BmgT120I GGNCC 2 cut(s) 549, 1067
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 4 cut(s) 173, 1025, 1071, 1299
BmrI ACTGGG 1 cut(s) 1152
BmsI GCATC 2 cut(s) 496, 857
BmuI ACTGGG 1 cut(s) 1152
BpmI CTGGAG 1 cut(s) 989
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 365, 475
BpuMI CCSGG 1 cut(s) 1025
Bsa29I ATCGAT 2 cut(s) 892, 896
BsaJI CCNNGG 2 cut(s) 180, 531
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 1101, 1131
Bsc4I CCNNNNNNNGG 2 cut(s) 991, 1030
Bse1I ACTGG 4 cut(s) 832, 858, 1006, 1147
Bse3DI GCAATG 2 cut(s) 981, 1202
BseBI CCWGG 3 cut(s) 173, 1071, 1299
BseCI ATCGAT 2 cut(s) 892, 896
BseDI CCNNGG 2 cut(s) 180, 531
BseGI GGATG 4 cut(s) 511, 817, 985, 1198
BseLI CCNNNNNNNGG 2 cut(s) 991, 1030
BseMI GCAATG 2 cut(s) 981, 1202
BseMII CTCAG 2 cut(s) 108, 732
BseNI ACTGG 4 cut(s) 832, 858, 1006, 1147
BseRI GAGGAG 2 cut(s) 58, 627
BseSI GKGCMC 2 cut(s) 438, 502
BsgI GTGCAG 2 cut(s) 753, 837
Bsh1236I CGCG 2 cut(s) 168, 774
Bsh1285I CGRYCG 2 cut(s) 115, 896
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 2 cut(s) 892, 896
BsiEI CGRYCG 2 cut(s) 115, 896
BsiHKAI GWGCWC 2 cut(s) 438, 502
BsiSI CCGG 3 cut(s) 212, 503, 1025
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 991, 1030
BsmAI GTCTC 3 cut(s) 31, 211, 753
BsmBI CGTCTC 2 cut(s) 31, 211
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 438, 502
Bsp143I GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
BspACI CCGC 6 cut(s) 31, 40, 168, 446, 774, 845
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 107, 733
BspDI ATCGAT 2 cut(s) 892, 896
BspFNI CGCG 2 cut(s) 168, 774
BspHI TCATGA 2 cut(s) 801, 1171
BspLI GGNNCC 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 3 cut(s) 776, 934, 1276
BspQI GCTCTTC 1 cut(s) 246
BsrDI GCAATG 2 cut(s) 981, 1202
BsrI ACTGG 4 cut(s) 832, 858, 1006, 1147
BssECI CCNNGG 2 cut(s) 180, 531
BssMI GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
BssNAI GTATAC 1 cut(s) 1304
BssT1I CCWWGG 1 cut(s) 531
Bst1107I GTATAC 1 cut(s) 1304
Bst2UI CCWGG 3 cut(s) 173, 1071, 1299
Bst4CI ACNGT 2 cut(s) 329, 1167
Bst6I CTCTTC 3 cut(s) 246, 705, 1227
BstAPI GCANNNNNTGC 3 cut(s) 48, 265, 506
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 94, 741
BstF5I GGATG 4 cut(s) 511, 817, 985, 1198
BstFNI CGCG 2 cut(s) 168, 774
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 3 cut(s) 168, 466, 774
BstKTI GATC 6 cut(s) 115, 784, 892, 896, 942, 1284
BstMAI GTCTC 3 cut(s) 31, 211, 753
BstMBI GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
BstMCI CGRYCG 2 cut(s) 115, 896
BstNI CCWGG 3 cut(s) 173, 1071, 1299
BstNSI RCATGY 2 cut(s) 959, 1313
BstSCI CCNGG 4 cut(s) 171, 1023, 1069, 1297
BstSLI GKGCMC 2 cut(s) 438, 502
BstUI CGCG 2 cut(s) 168, 774
BstX2I RGATCY 1 cut(s) 1281
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 1 cut(s) 1281
BstZ17I GTATAC 1 cut(s) 1304
Bsu15I ATCGAT 2 cut(s) 892, 896
BsuI GTATCC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 2 cut(s) 892, 896
BtgZI GCGATG 1 cut(s) 257
BtsCI GGATG 4 cut(s) 511, 817, 985, 1198
BtsI GCAGTG 2 cut(s) 46, 867
BtsIMutI CAGTG 3 cut(s) 46, 851, 867
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 498
CaiI CAGNNNCTG 1 cut(s) 740
CciI TCATGA 2 cut(s) 801, 1171
CfoI GCGC 3 cut(s) 168, 466, 774
Cfr13I GGNCC 2 cut(s) 549, 1067
ClaI ATCGAT 2 cut(s) 892, 896
CseI GACGC 1 cut(s) 109
CspCI CAANNNNNGTGG 4 cut(s) 602, 637, 775, 810
CviAII CATG 9 cut(s) 413, 605, 748, 802, 956, 986, 1172, 1310, 1350
DdeI CTNAG 2 cut(s) 94, 741
DpnI GATC 6 cut(s) 114, 783, 891, 895, 941, 1283
DpnII GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
Eam1104I CTCTTC 3 cut(s) 246, 705, 1227
EarI CTCTTC 3 cut(s) 246, 705, 1227
Eco130I CCWWGG 1 cut(s) 531
Eco32I GATATC 2 cut(s) 558, 1318
Eco47I GGWCC 2 cut(s) 549, 1067
Eco57I CTGAAG 3 cut(s) 37, 447, 1063
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 3 cut(s) 171, 1069, 1297
EcoRV GATATC 2 cut(s) 558, 1318
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 2 cut(s) 31, 211
FaeI CATG 9 cut(s) 416, 608, 751, 805, 959, 989, 1175, 1313, 1353
FaqI GGGAC 1 cut(s) 107
FatI CATG 9 cut(s) 412, 604, 747, 801, 955, 985, 1171, 1309, 1349
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1303
FokI GGATG 4 cut(s) 518, 824, 992, 1205
FspBI CTAG 3 cut(s) 288, 408, 1137
GlaI GCGC 3 cut(s) 167, 465, 773
GsuI CTGGAG 1 cut(s) 989
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 1 cut(s) 171
HapII CCGG 3 cut(s) 212, 503, 1025
HgaI GACGC 1 cut(s) 109
HhaI GCGC 3 cut(s) 168, 466, 774
Hin1II CATG 9 cut(s) 416, 608, 751, 805, 959, 989, 1175, 1313, 1353
Hin6I GCGC 3 cut(s) 166, 464, 772
HinP1I GCGC 3 cut(s) 166, 464, 772
HincII GTYRAC 1 cut(s) 248
HindII GTYRAC 1 cut(s) 248
HinfI GANTC 3 cut(s) 949, 1034, 1074
HpaII CCGG 3 cut(s) 212, 503, 1025
HphI GGTGA 3 cut(s) 313, 1072, 1241
Hpy166II GTNNAC 5 cut(s) 248, 436, 500, 624, 1304
Hpy188I TCNGA 3 cut(s) 97, 939, 1082
Hpy188III TCNNGA 3 cut(s) 724, 802, 1172
Hpy8I GTNNAC 5 cut(s) 248, 436, 500, 624, 1304
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 6 cut(s) 44, 157, 683, 991, 1183, 1270
HpyCH4III ACNGT 2 cut(s) 329, 1167
HpyCH4IV ACGT 1 cut(s) 8
HpyF3I CTNAG 2 cut(s) 94, 741
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 9 cut(s) 416, 608, 751, 805, 959, 989, 1175, 1313, 1353
HspAI GCGC 3 cut(s) 166, 464, 772
Kzo9I GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 5 cut(s) 71, 139, 149, 190, 273
LweI GCATC 2 cut(s) 496, 857
MaeI CTAG 3 cut(s) 288, 408, 1137
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 3 cut(s) 340, 823, 1240
MalI GATC 6 cut(s) 114, 783, 891, 895, 941, 1283
MboI GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
MboII GAAGA 7 cut(s) 216, 233, 316, 692, 1069, 1163, 1244
MflI RGATCY 1 cut(s) 1281
MhlI GDGCHC 2 cut(s) 438, 502
MluCI AATT 5 cut(s) 74, 311, 752, 882, 1126
MseI TTAA 2 cut(s) 1086, 1209
MslI CAYNNNNRTG 2 cut(s) 355, 519
MspA1I CMGCKG 2 cut(s) 737, 821
MspI CCGG 3 cut(s) 212, 503, 1025
MspR9I CCNGG 4 cut(s) 173, 1025, 1071, 1299
MvaI CCWGG 3 cut(s) 173, 1071, 1299
MvnI CGCG 2 cut(s) 168, 774
NciI CCSGG 1 cut(s) 1025
NdeII GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
NlaIII CATG 9 cut(s) 416, 608, 751, 805, 959, 989, 1175, 1313, 1353
NlaIV GGNNCC 1 cut(s) 186
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 2 cut(s) 340, 1240
NspI RCATGY 2 cut(s) 959, 1313
PagI TCATGA 2 cut(s) 801, 1171
PaqCI CACCTGC 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 3 cut(s) 949, 1034, 1074
PflMI CCANNNNNTGG 1 cut(s) 991
PfoI TCCNGGA 1 cut(s) 1069
Ple19I CGATCG 2 cut(s) 115, 896
Psp6I CCWGG 3 cut(s) 171, 1069, 1297
PspGI CCWGG 3 cut(s) 171, 1069, 1297
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 2 cut(s) 549, 1067
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 1 cut(s) 1281
PvuI CGATCG 2 cut(s) 115, 896
PvuII CAGCTG 2 cut(s) 737, 821
RseI CAYNNNNRTG 2 cut(s) 355, 519
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 1086, 1209
Sau3AI GATC 6 cut(s) 112, 781, 889, 893, 939, 1281
Sau96I GGNCC 2 cut(s) 549, 1067
ScrFI CCNGG 4 cut(s) 173, 1025, 1071, 1299
SduI GDGCHC 2 cut(s) 438, 502
SfaNI GCATC 2 cut(s) 496, 857
SinI GGWCC 2 cut(s) 549, 1067
SmiMI CAYNNNNRTG 2 cut(s) 355, 519
SmlI CTYRAG 2 cut(s) 380, 490
SmoI CTYRAG 2 cut(s) 380, 490
Sse9I AATT 5 cut(s) 74, 311, 752, 882, 1126
SsiI CCGC 6 cut(s) 31, 40, 168, 446, 774, 845
SspI AATATT 1 cut(s) 646
SspMI CTAG 3 cut(s) 288, 408, 1137
StyD4I CCNGG 4 cut(s) 171, 1023, 1069, 1297
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 2 cut(s) 329, 1167
TaiI ACGT 1 cut(s) 11
TaqI TCGA 6 cut(s) 194, 401, 892, 896, 952, 1320
TasI AATT 5 cut(s) 74, 311, 752, 882, 1126
TauI GCSGC 3 cut(s) 42, 171, 848
TfiI GAWTC 3 cut(s) 949, 1034, 1074
Tru1I TTAA 2 cut(s) 1086, 1209
Tru9I TTAA 2 cut(s) 1086, 1209
TscAI CASTG 3 cut(s) 53, 858, 867
TseFI GTSAC 2 cut(s) 340, 1240
Tsp45I GTSAC 2 cut(s) 340, 1240
TspDTI ATGAA 4 cut(s) 593, 764, 1160, 1188
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 3 cut(s) 53, 858, 867
Van91I CCANNNNNTGG 1 cut(s) 991
VneI GTGCAC 2 cut(s) 434, 498
VpaK11BI GGWCC 2 cut(s) 549, 1067
XapI RAATTY 1 cut(s) 311
XceI RCATGY 2 cut(s) 959, 1313
XmiI GTMKAC 1 cut(s) 1303
XspI CTAG 3 cut(s) 288, 408, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.