Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1206 |
| AccI | GTMKAC | 1 cut(s) | 1083 |
| AciI | CCGC | 6 cut(s) | 215, 234, 410, 517, 681, 819 |
| AclI | AACGTT | 1 cut(s) | 269 |
| AclWI | GGATC | 5 cut(s) | 886, 898, 987, 1000, 1133 |
| AcsI | RAATTY | 4 cut(s) | 354, 477, 687, 1046 |
| AcuI | CTGAAG | 1 cut(s) | 82 |
| AdeI | CACNNNGTG | 1 cut(s) | 1206 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 169, 563, 1206, 1365 |
| AflIII | ACRYGT | 2 cut(s) | 427, 741 |
| AgsI | TTSAA | 9 cut(s) | 202, 453, 467, 713, 723, 837, 1228, 1241, 1343 |
| AluBI | AGCT | 8 cut(s) | 56, 102, 224, 387, 815, 857, 914, 1014 |
| AluI | AGCT | 8 cut(s) | 56, 102, 224, 387, 815, 857, 914, 1014 |
| Alw21I | GWGCWC | 1 cut(s) | 541 |
| Alw26I | GTCTC | 1 cut(s) | 865 |
| AlwI | GGATC | 5 cut(s) | 886, 898, 987, 1000, 1133 |
| ApoI | RAATTY | 4 cut(s) | 354, 477, 687, 1046 |
| AseI | ATTAAT | 1 cut(s) | 510 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 12, 355, 1050 |
| AspLEI | GCGC | 3 cut(s) | 134, 440, 1250 |
| AspS9I | GGNCC | 2 cut(s) | 394, 1197 |
| AsuHPI | GGTGA | 1 cut(s) | 579 |
| AsuNHI | GCTAGC | 1 cut(s) | 815 |
| AvaII | GGWCC | 2 cut(s) | 394, 1197 |
| BamHI | GGATCC | 1 cut(s) | 992 |
| BauI | CACGAG | 1 cut(s) | 1313 |
| BbsI | GAAGAC | 3 cut(s) | 268, 955, 1057 |
| Bbv12I | GWGCWC | 1 cut(s) | 541 |
| BbvCI | CCTCAGC | 1 cut(s) | 68 |
| BccI | CCATC | 3 cut(s) | 588, 936, 1270 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1173, 1207 |
| BcoDI | GTCTC | 1 cut(s) | 865 |
| BfaI | CTAG | 3 cut(s) | 384, 770, 816 |
| BfoI | RGCGCY | 1 cut(s) | 135 |
| BisI | GCNGC | 1 cut(s) | 681 |
| BlsI | GCNGC | 1 cut(s) | 682 |
| Bme18I | GGWCC | 2 cut(s) | 394, 1197 |
| BmgT120I | GGNCC | 2 cut(s) | 394, 1197 |
| BmiI | GGNNCC | 4 cut(s) | 193, 396, 994, 1258 |
| BmrI | ACTGGG | 1 cut(s) | 1212 |
| BmsI | GCATC | 5 cut(s) | 375, 649, 649, 1299, 1364 |
| BmtI | GCTAGC | 1 cut(s) | 819 |
| BmuI | ACTGGG | 1 cut(s) | 1212 |
| BpiI | GAAGAC | 3 cut(s) | 268, 955, 1057 |
| BpmI | CTGGAG | 1 cut(s) | 831 |
| Bpu10I | CCTNAGC | 1 cut(s) | 68 |
| BsaAI | YACGTR | 1 cut(s) | 391 |
| BsaJI | CCNNGG | 2 cut(s) | 253, 1121 |
| BsaWI | WCCGGW | 1 cut(s) | 580 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 201, 231, 942, 972 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 169, 563, 1206, 1365 |
| Bse1I | ACTGG | 4 cut(s) | 557, 729, 1055, 1207 |
| Bse3DI | GCAATG | 2 cut(s) | 312, 1292 |
| BseDI | CCNNGG | 2 cut(s) | 253, 1121 |
| BseGI | GGATG | 4 cut(s) | 113, 249, 1314, 1355 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 169, 563, 1206, 1365 |
| BseMI | GCAATG | 2 cut(s) | 312, 1292 |
| BseMII | CTCAG | 2 cut(s) | 82, 1128 |
| BseNI | ACTGG | 4 cut(s) | 557, 729, 1055, 1207 |
| BsiHKAI | GWGCWC | 1 cut(s) | 541 |
| BsiSI | CCGG | 3 cut(s) | 129, 581, 1319 |
| BslFI | GGGAC | 1 cut(s) | 380 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 169, 563, 1206, 1365 |
| BsmAI | GTCTC | 1 cut(s) | 865 |
| BsmFI | GGGAC | 1 cut(s) | 380 |
| Bsp1286I | GDGCHC | 1 cut(s) | 541 |
| Bsp143I | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| BspACI | CCGC | 6 cut(s) | 215, 234, 410, 517, 681, 819 |
| BspCNI | CTCAG | 2 cut(s) | 81, 1129 |
| BspHI | TCATGA | 1 cut(s) | 147 |
| BspLI | GGNNCC | 4 cut(s) | 193, 396, 994, 1258 |
| BspOI | GCTAGC | 1 cut(s) | 819 |
| BspPI | GGATC | 5 cut(s) | 886, 898, 987, 1000, 1133 |
| BspQI | GCTCTTC | 1 cut(s) | 546 |
| BsrDI | GCAATG | 2 cut(s) | 312, 1292 |
| BsrI | ACTGG | 4 cut(s) | 557, 729, 1055, 1207 |
| BssECI | CCNNGG | 2 cut(s) | 253, 1121 |
| BssMI | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| BssSI | CACGAG | 1 cut(s) | 1313 |
| BssT1I | CCWWGG | 2 cut(s) | 253, 1121 |
| Bst2BI | CACGAG | 1 cut(s) | 1313 |
| Bst4CI | ACNGT | 3 cut(s) | 473, 505, 1335 |
| Bst6I | CTCTTC | 1 cut(s) | 546 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 446 |
| BstBAI | YACGTR | 1 cut(s) | 391 |
| BstC8I | GCNNGC | 4 cut(s) | 280, 817, 859, 1353 |
| BstDEI | CTNAG | 3 cut(s) | 68, 647, 1137 |
| BstF5I | GGATG | 4 cut(s) | 113, 249, 1314, 1355 |
| BstH2I | RGCGCY | 1 cut(s) | 135 |
| BstHHI | GCGC | 3 cut(s) | 134, 440, 1250 |
| BstKTI | GATC | 6 cut(s) | 43, 564, 881, 906, 995, 1128 |
| BstMAI | GTCTC | 1 cut(s) | 865 |
| BstMBI | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 140, 221, 384, 446 |
| BstNSI | RCATGY | 3 cut(s) | 282, 431, 745 |
| BstV2I | GAAGAC | 3 cut(s) | 268, 955, 1057 |
| BstX2I | RGATCY | 1 cut(s) | 992 |
| BstYI | RGATCY | 1 cut(s) | 992 |
| BtgZI | GCGATG | 2 cut(s) | 626, 1289 |
| BtsCI | GGATG | 4 cut(s) | 113, 249, 1314, 1355 |
| BtsIMutI | CAGTG | 3 cut(s) | 478, 564, 1200 |
| Cac8I | GCNNGC | 4 cut(s) | 280, 817, 859, 1353 |
| CciI | TCATGA | 1 cut(s) | 147 |
| CfoI | GCGC | 3 cut(s) | 134, 440, 1250 |
| Cfr13I | GGNCC | 2 cut(s) | 394, 1197 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 945, 980 |
| CviAII | CATG | 6 cut(s) | 23, 148, 279, 428, 546, 742 |
| DdeI | CTNAG | 3 cut(s) | 68, 647, 1137 |
| DpnI | GATC | 6 cut(s) | 42, 563, 880, 905, 994, 1127 |
| DpnII | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| DraIII | CACNNNGTG | 1 cut(s) | 1206 |
| Eam1104I | CTCTTC | 1 cut(s) | 546 |
| EarI | CTCTTC | 1 cut(s) | 546 |
| Eco130I | CCWWGG | 2 cut(s) | 253, 1121 |
| Eco47I | GGWCC | 2 cut(s) | 394, 1197 |
| Eco57I | CTGAAG | 1 cut(s) | 82 |
| EcoT14I | CCWWGG | 2 cut(s) | 253, 1121 |
| ErhI | CCWWGG | 2 cut(s) | 253, 1121 |
| FaeI | CATG | 6 cut(s) | 26, 151, 282, 431, 549, 745 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 29, 32, 317, 349, 343, 375, 1344, 1376 |
| FaqI | GGGAC | 1 cut(s) | 380 |
| FatI | CATG | 6 cut(s) | 22, 147, 278, 427, 545, 741 |
| FauI | CCCGC | 1 cut(s) | 510 |
| FblI | GTMKAC | 1 cut(s) | 1083 |
| Fnu4HI | GCNGC | 1 cut(s) | 681 |
| FokI | GGATG | 4 cut(s) | 120, 236, 1321, 1342 |
| Fsp4HI | GCNGC | 1 cut(s) | 681 |
| FspBI | CTAG | 3 cut(s) | 384, 770, 816 |
| GlaI | GCGC | 3 cut(s) | 133, 439, 1249 |
| GluI | GCNGC | 1 cut(s) | 681 |
| GsuI | CTGGAG | 1 cut(s) | 831 |
| HaeII | RGCGCY | 1 cut(s) | 135 |
| HapII | CCGG | 3 cut(s) | 129, 581, 1319 |
| HhaI | GCGC | 3 cut(s) | 134, 440, 1250 |
| Hin1II | CATG | 6 cut(s) | 26, 151, 282, 431, 549, 745 |
| Hin6I | GCGC | 3 cut(s) | 132, 438, 1248 |
| HinP1I | GCGC | 3 cut(s) | 132, 438, 1248 |
| HindIII | AAGCTT | 1 cut(s) | 912 |
| HinfI | GANTC | 4 cut(s) | 584, 719, 928, 1033 |
| HpaII | CCGG | 3 cut(s) | 129, 581, 1319 |
| HphI | GGTGA | 1 cut(s) | 579 |
| Hpy166II | GTNNAC | 2 cut(s) | 1084, 1197 |
| Hpy188III | TCNNGA | 4 cut(s) | 148, 810, 901, 1043 |
| Hpy8I | GTNNAC | 2 cut(s) | 1084, 1197 |
| Hpy99I | CGWCG | 1 cut(s) | 48 |
| HpyAV | CCTTC | 1 cut(s) | 106 |
| HpyCH4III | ACNGT | 3 cut(s) | 473, 505, 1335 |
| HpyCH4IV | ACGT | 3 cut(s) | 269, 390, 1019 |
| HpyCH4V | TGCA | 7 cut(s) | 143, 294, 323, 640, 1100, 1184, 1351 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 140, 221, 384, 446 |
| HpyF3I | CTNAG | 3 cut(s) | 68, 647, 1137 |
| HpySE526I | ACGT | 3 cut(s) | 269, 390, 1019 |
| Hsp92II | CATG | 6 cut(s) | 26, 151, 282, 431, 549, 745 |
| HspAI | GCGC | 3 cut(s) | 132, 438, 1248 |
| Kzo9I | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| LguI | GCTCTTC | 1 cut(s) | 546 |
| LmnI | GCTCC | 3 cut(s) | 221, 812, 1245 |
| LweI | GCATC | 5 cut(s) | 375, 649, 649, 1299, 1364 |
| MaeI | CTAG | 3 cut(s) | 384, 770, 816 |
| MaeII | ACGT | 3 cut(s) | 269, 390, 1019 |
| MaeIII | GTNAC | 2 cut(s) | 737, 1020 |
| MalI | GATC | 6 cut(s) | 42, 563, 880, 905, 994, 1127 |
| MboI | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| MflI | RGATCY | 1 cut(s) | 992 |
| MhlI | GDGCHC | 1 cut(s) | 541 |
| MmeI | TCCRAC | 2 cut(s) | 757, 958 |
| MnlI | CCTC | 4 cut(s) | 77, 203, 644, 1106 |
| MroXI | GAANNNNTTC | 3 cut(s) | 12, 355, 1050 |
| MseI | TTAA | 4 cut(s) | 510, 618, 788, 1187 |
| MslI | CAYNNNNRTG | 4 cut(s) | 371, 432, 557, 592 |
| MspA1I | CMGCKG | 1 cut(s) | 683 |
| MspI | CCGG | 3 cut(s) | 129, 581, 1319 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 140, 221, 384, 446 |
| NdeII | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| NheI | GCTAGC | 1 cut(s) | 815 |
| NlaIII | CATG | 6 cut(s) | 26, 151, 282, 431, 549, 745 |
| NlaIV | GGNNCC | 4 cut(s) | 193, 396, 994, 1258 |
| NmuCI | GTSAC | 1 cut(s) | 737 |
| NspI | RCATGY | 3 cut(s) | 282, 431, 745 |
| PaeI | GCATGC | 1 cut(s) | 282 |
| PagI | TCATGA | 1 cut(s) | 147 |
| PciI | ACATGT | 2 cut(s) | 427, 741 |
| PciSI | GCTCTTC | 1 cut(s) | 546 |
| PdmI | GAANNNNTTC | 3 cut(s) | 12, 355, 1050 |
| PfeI | GAWTC | 4 cut(s) | 584, 719, 928, 1033 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1206 |
| PkrI | GCNGC | 1 cut(s) | 682 |
| Ppu21I | YACGTR | 1 cut(s) | 391 |
| PscI | ACATGT | 2 cut(s) | 427, 741 |
| PshBI | ATTAAT | 1 cut(s) | 510 |
| Psp1406I | AACGTT | 1 cut(s) | 269 |
| PspN4I | GGNNCC | 4 cut(s) | 193, 396, 994, 1258 |
| PspPI | GGNCC | 2 cut(s) | 394, 1197 |
| PsuI | RGATCY | 1 cut(s) | 992 |
| RseI | CAYNNNNRTG | 4 cut(s) | 371, 432, 557, 592 |
| SapI | GCTCTTC | 1 cut(s) | 546 |
| SaqAI | TTAA | 4 cut(s) | 510, 618, 788, 1187 |
| SatI | GCNGC | 1 cut(s) | 681 |
| Sau3AI | GATC | 6 cut(s) | 40, 561, 878, 903, 992, 1125 |
| Sau96I | GGNCC | 2 cut(s) | 394, 1197 |
| SduI | GDGCHC | 1 cut(s) | 541 |
| SfaNI | GCATC | 5 cut(s) | 375, 649, 649, 1299, 1364 |
| SinI | GGWCC | 2 cut(s) | 394, 1197 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 371, 432, 557, 592 |
| SphI | GCATGC | 1 cut(s) | 282 |
| SsiI | CCGC | 6 cut(s) | 215, 234, 410, 517, 681, 819 |
| SspI | AATATT | 1 cut(s) | 1075 |
| SspMI | CTAG | 3 cut(s) | 384, 770, 816 |
| StyI | CCWWGG | 2 cut(s) | 253, 1121 |
| TaaI | ACNGT | 3 cut(s) | 473, 505, 1335 |
| TaiI | ACGT | 3 cut(s) | 272, 393, 1022 |
| TauI | GCSGC | 1 cut(s) | 683 |
| TfiI | GAWTC | 4 cut(s) | 584, 719, 928, 1033 |
| Tru1I | TTAA | 4 cut(s) | 510, 618, 788, 1187 |
| Tru9I | TTAA | 4 cut(s) | 510, 618, 788, 1187 |
| TscAI | CASTG | 3 cut(s) | 478, 564, 1207 |
| TseFI | GTSAC | 1 cut(s) | 737 |
| Tsp45I | GTSAC | 1 cut(s) | 737 |
| TspDTI | ATGAA | 7 cut(s) | 39, 164, 541, 565, 690, 1046, 1057 |
| TspRI | CASTG | 3 cut(s) | 478, 564, 1207 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1206 |
| VpaK11BI | GGWCC | 2 cut(s) | 394, 1197 |
| VspI | ATTAAT | 1 cut(s) | 510 |
| XapI | RAATTY | 4 cut(s) | 354, 477, 687, 1046 |
| XceI | RCATGY | 3 cut(s) | 282, 431, 745 |
| XmiI | GTMKAC | 1 cut(s) | 1083 |
| XmnI | GAANNNNTTC | 3 cut(s) | 12, 355, 1050 |
| XspI | CTAG | 3 cut(s) | 384, 770, 816 |