Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1160 |
| Acc65I | GGTACC | 1 cut(s) | 454 |
| AccB1I | GGYRCC | 1 cut(s) | 454 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 573 |
| AccI | GTMKAC | 3 cut(s) | 610, 673, 758 |
| AciI | CCGC | 2 cut(s) | 105, 423 |
| AcsI | RAATTY | 1 cut(s) | 618 |
| AcuI | CTGAAG | 1 cut(s) | 492 |
| AfaI | GTAC | 3 cut(s) | 355, 456, 464 |
| AfeI | AGCGCT | 1 cut(s) | 1104 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 573, 654 |
| AgsI | TTSAA | 6 cut(s) | 255, 689, 734, 908, 924, 1285 |
| AhlI | ACTAGT | 1 cut(s) | 113 |
| AjnI | CCWGG | 4 cut(s) | 572, 604, 1178, 1262 |
| AleI | CACNNNNGTG | 2 cut(s) | 116, 137 |
| AluBI | AGCT | 9 cut(s) | 191, 326, 512, 531, 692, 884, 897, 1305, 1369 |
| AluI | AGCT | 9 cut(s) | 191, 326, 512, 531, 692, 884, 897, 1305, 1369 |
| Alw21I | GWGCWC | 4 cut(s) | 58, 134, 987, 1307 |
| Alw26I | GTCTC | 1 cut(s) | 779 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 194 |
| Aor51HI | AGCGCT | 1 cut(s) | 1104 |
| AoxI | GGCC | 1 cut(s) | 141 |
| ApeKI | GCWGC | 4 cut(s) | 191, 587, 856, 1031 |
| ApoI | RAATTY | 1 cut(s) | 618 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 693 |
| Asp718I | GGTACC | 1 cut(s) | 454 |
| AspLEI | GCGC | 2 cut(s) | 579, 1105 |
| AspS9I | GGNCC | 5 cut(s) | 142, 220, 487, 682, 1084 |
| AvaII | GGWCC | 4 cut(s) | 220, 487, 682, 1084 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 446, 479 |
| BanI | GGYRCC | 1 cut(s) | 454 |
| BanII | GRGCYC | 1 cut(s) | 1307 |
| BbsI | GAAGAC | 3 cut(s) | 219, 418, 1012 |
| Bbv12I | GWGCWC | 4 cut(s) | 58, 134, 987, 1307 |
| BbvI | GCAGC | 4 cut(s) | 178, 599, 868, 1018 |
| BccI | CCATC | 3 cut(s) | 74, 648, 1431 |
| BceAI | ACGGC | 1 cut(s) | 863 |
| BciT130I | CCWGG | 4 cut(s) | 574, 606, 1180, 1264 |
| BciVI | GTATCC | 1 cut(s) | 954 |
| BcoDI | GTCTC | 1 cut(s) | 779 |
| BcuI | ACTAGT | 1 cut(s) | 113 |
| BfaI | CTAG | 3 cut(s) | 114, 623, 1397 |
| BfmI | CTRYAG | 4 cut(s) | 174, 357, 372, 854 |
| BfoI | RGCGCY | 1 cut(s) | 1106 |
| BfuAI | ACCTGC | 1 cut(s) | 1160 |
| BfuI | GTATCC | 1 cut(s) | 954 |
| BisI | GCNGC | 4 cut(s) | 192, 588, 857, 1032 |
| BlsI | GCNGC | 4 cut(s) | 193, 589, 858, 1033 |
| BmcAI | AGTACT | 1 cut(s) | 355 |
| Bme1390I | CCNGG | 4 cut(s) | 574, 606, 1180, 1264 |
| Bme18I | GGWCC | 4 cut(s) | 220, 487, 682, 1084 |
| BmgT120I | GGNCC | 5 cut(s) | 142, 220, 487, 682, 1084 |
| BmiI | GGNNCC | 1 cut(s) | 456 |
| BmrFI | CCNGG | 4 cut(s) | 574, 606, 1180, 1264 |
| BmrI | ACTGGG | 1 cut(s) | 1199 |
| BmsI | GCATC | 3 cut(s) | 901, 1157, 1421 |
| BmuI | ACTGGG | 1 cut(s) | 1199 |
| BpiI | GAAGAC | 3 cut(s) | 219, 418, 1012 |
| BpuEI | CTTGAG | 2 cut(s) | 403, 1066 |
| BsaAI | YACGTR | 1 cut(s) | 451 |
| BsaJI | CCNNGG | 6 cut(s) | 45, 483, 582, 1178, 1179, 1383 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 573, 654 |
| Bse118I | RCCGGY | 2 cut(s) | 164, 914 |
| Bse1I | ACTGG | 5 cut(s) | 458, 951, 1026, 1093, 1205 |
| Bse3DI | GCAATG | 1 cut(s) | 788 |
| BseBI | CCWGG | 4 cut(s) | 574, 606, 1180, 1264 |
| BseDI | CCNNGG | 6 cut(s) | 45, 483, 582, 1178, 1179, 1383 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 573, 654 |
| BseMI | GCAATG | 1 cut(s) | 788 |
| BseMII | CTCAG | 4 cut(s) | 255, 505, 1320, 1344 |
| BseNI | ACTGG | 5 cut(s) | 458, 951, 1026, 1093, 1205 |
| BseRI | GAGGAG | 4 cut(s) | 82, 85, 88, 91 |
| BseXI | GCAGC | 4 cut(s) | 178, 599, 868, 1018 |
| Bsh1285I | CGRYCG | 1 cut(s) | 319 |
| BshFI | GGCC | 1 cut(s) | 143 |
| BshNI | GGYRCC | 1 cut(s) | 454 |
| BsiEI | CGRYCG | 1 cut(s) | 319 |
| BsiHKAI | GWGCWC | 4 cut(s) | 58, 134, 987, 1307 |
| BsiSI | CCGG | 2 cut(s) | 165, 915 |
| BslFI | GGGAC | 2 cut(s) | 631, 949 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 573, 654 |
| BsmAI | GTCTC | 1 cut(s) | 779 |
| BsmFI | GGGAC | 2 cut(s) | 631, 949 |
| BsmI | GAATGC | 1 cut(s) | 394 |
| BsnI | GGCC | 1 cut(s) | 143 |
| Bsp1286I | GDGCHC | 4 cut(s) | 58, 134, 987, 1307 |
| Bsp1407I | TGTACA | 1 cut(s) | 462 |
| Bsp143I | GATC | 1 cut(s) | 307 |
| Bsp19I | CCATGG | 2 cut(s) | 45, 582 |
| BspACI | CCGC | 2 cut(s) | 105, 423 |
| BspANI | GGCC | 1 cut(s) | 143 |
| BspCNI | CTCAG | 4 cut(s) | 254, 504, 1319, 1343 |
| BspLI | GGNNCC | 1 cut(s) | 456 |
| BspMAI | CTGCAG | 2 cut(s) | 361, 858 |
| BspMI | ACCTGC | 1 cut(s) | 1160 |
| BspT107I | GGYRCC | 1 cut(s) | 454 |
| BsrDI | GCAATG | 1 cut(s) | 788 |
| BsrFI | RCCGGY | 2 cut(s) | 164, 914 |
| BsrGI | TGTACA | 1 cut(s) | 462 |
| BsrI | ACTGG | 5 cut(s) | 458, 951, 1026, 1093, 1205 |
| BssAI | RCCGGY | 2 cut(s) | 164, 914 |
| BssECI | CCNNGG | 6 cut(s) | 45, 483, 582, 1178, 1179, 1383 |
| BssMI | GATC | 1 cut(s) | 307 |
| BssNAI | GTATAC | 1 cut(s) | 759 |
| BssT1I | CCWWGG | 4 cut(s) | 45, 483, 582, 1383 |
| Bst1107I | GTATAC | 1 cut(s) | 759 |
| Bst2UI | CCWGG | 4 cut(s) | 574, 606, 1180, 1264 |
| Bst4CI | ACNGT | 2 cut(s) | 849, 1343 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 389, 596 |
| BstAUI | TGTACA | 1 cut(s) | 462 |
| BstBAI | YACGTR | 1 cut(s) | 451 |
| BstC8I | GCNNGC | 2 cut(s) | 660, 820 |
| BstDEI | CTNAG | 6 cut(s) | 62, 241, 491, 707, 1306, 1330 |
| BstDSI | CCRYGG | 2 cut(s) | 45, 582 |
| BstH2I | RGCGCY | 1 cut(s) | 1106 |
| BstHHI | GCGC | 2 cut(s) | 579, 1105 |
| BstKTI | GATC | 1 cut(s) | 310 |
| BstMAI | GTCTC | 1 cut(s) | 779 |
| BstMBI | GATC | 1 cut(s) | 307 |
| BstMCI | CGRYCG | 1 cut(s) | 319 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 389, 429, 596 |
| BstNI | CCWGG | 4 cut(s) | 574, 606, 1180, 1264 |
| BstNSI | RCATGY | 1 cut(s) | 1135 |
| BstSCI | CCNGG | 4 cut(s) | 572, 604, 1178, 1262 |
| BstSFI | CTRYAG | 4 cut(s) | 174, 357, 372, 854 |
| BstV1I | GCAGC | 4 cut(s) | 178, 599, 868, 1018 |
| BstV2I | GAAGAC | 3 cut(s) | 219, 418, 1012 |
| BstZ17I | GTATAC | 1 cut(s) | 759 |
| BsuI | GTATCC | 1 cut(s) | 954 |
| BsuRI | GGCC | 1 cut(s) | 143 |
| BtgI | CCRYGG | 2 cut(s) | 45, 582 |
| BtsIMutI | CAGTG | 4 cut(s) | 465, 944, 1100, 1338 |
| BveI | ACCTGC | 1 cut(s) | 1160 |
| Cac8I | GCNNGC | 2 cut(s) | 660, 820 |
| CaiI | CAGNNNCTG | 1 cut(s) | 194 |
| CfoI | GCGC | 2 cut(s) | 579, 1105 |
| Cfr10I | RCCGGY | 2 cut(s) | 164, 914 |
| Cfr13I | GGNCC | 5 cut(s) | 142, 220, 487, 682, 1084 |
| CseI | GACGC | 1 cut(s) | 421 |
| Csp6I | GTAC | 3 cut(s) | 354, 455, 463 |
| CviAII | CATG | 6 cut(s) | 31, 46, 583, 751, 1132, 1165 |
| CviQI | GTAC | 3 cut(s) | 354, 455, 463 |
| DdeI | CTNAG | 6 cut(s) | 62, 241, 491, 707, 1306, 1330 |
| DpnI | GATC | 1 cut(s) | 309 |
| DpnII | GATC | 1 cut(s) | 307 |
| Ecl136II | GAGCTC | 1 cut(s) | 1305 |
| Eco130I | CCWWGG | 4 cut(s) | 45, 483, 582, 1383 |
| Eco24I | GRGCYC | 1 cut(s) | 1307 |
| Eco47I | GGWCC | 4 cut(s) | 220, 487, 682, 1084 |
| Eco47III | AGCGCT | 1 cut(s) | 1104 |
| Eco53kI | GAGCTC | 1 cut(s) | 1305 |
| Eco57I | CTGAAG | 1 cut(s) | 492 |
| EcoICRI | GAGCTC | 1 cut(s) | 1305 |
| EcoO109I | RGGNCCY | 3 cut(s) | 487, 682, 1084 |
| EcoRII | CCWGG | 4 cut(s) | 572, 604, 1178, 1262 |
| EcoT14I | CCWWGG | 4 cut(s) | 45, 483, 582, 1383 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| EcoT38I | GRGCYC | 1 cut(s) | 1307 |
| ErhI | CCWWGG | 4 cut(s) | 45, 483, 582, 1383 |
| FaeI | CATG | 6 cut(s) | 34, 49, 586, 754, 1135, 1168 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 490, 522 |
| FaqI | GGGAC | 2 cut(s) | 631, 949 |
| FatI | CATG | 6 cut(s) | 30, 45, 582, 750, 1131, 1164 |
| FblI | GTMKAC | 3 cut(s) | 610, 673, 758 |
| Fnu4HI | GCNGC | 4 cut(s) | 192, 588, 857, 1032 |
| FriOI | GRGCYC | 1 cut(s) | 1307 |
| Fsp4HI | GCNGC | 4 cut(s) | 192, 588, 857, 1032 |
| FspBI | CTAG | 3 cut(s) | 114, 623, 1397 |
| GlaI | GCGC | 2 cut(s) | 578, 1104 |
| GluI | GCNGC | 4 cut(s) | 192, 588, 857, 1032 |
| HaeII | RGCGCY | 1 cut(s) | 1106 |
| HaeIII | GGCC | 1 cut(s) | 143 |
| HapII | CCGG | 2 cut(s) | 165, 915 |
| HgaI | GACGC | 1 cut(s) | 421 |
| HhaI | GCGC | 2 cut(s) | 579, 1105 |
| Hin1II | CATG | 6 cut(s) | 34, 49, 586, 754, 1135, 1168 |
| Hin6I | GCGC | 2 cut(s) | 577, 1103 |
| HinP1I | GCGC | 2 cut(s) | 577, 1103 |
| HindIII | AAGCTT | 1 cut(s) | 1367 |
| HinfI | GANTC | 5 cut(s) | 86, 197, 414, 501, 644 |
| HpaII | CCGG | 2 cut(s) | 165, 915 |
| Hpy166II | GTNNAC | 7 cut(s) | 155, 463, 611, 674, 759, 1175, 1210 |
| Hpy188I | TCNGA | 4 cut(s) | 494, 784, 1195, 1309 |
| Hpy188III | TCNNGA | 9 cut(s) | 201, 436, 641, 698, 734, 805, 958, 1082, 1397 |
| Hpy8I | GTNNAC | 7 cut(s) | 155, 463, 611, 674, 759, 1175, 1210 |
| Hpy99I | CGWCG | 1 cut(s) | 437 |
| HpyAV | CCTTC | 1 cut(s) | 1304 |
| HpyCH4III | ACNGT | 2 cut(s) | 849, 1343 |
| HpyCH4IV | ACGT | 1 cut(s) | 450 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 344, 359, 383, 856, 872, 974, 1148 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 389, 429, 596 |
| HpyF3I | CTNAG | 6 cut(s) | 62, 241, 491, 707, 1306, 1330 |
| HpySE526I | ACGT | 1 cut(s) | 450 |
| Hsp92II | CATG | 6 cut(s) | 34, 49, 586, 754, 1135, 1168 |
| HspAI | GCGC | 2 cut(s) | 577, 1103 |
| KpnI | GGTACC | 1 cut(s) | 458 |
| Kzo9I | GATC | 1 cut(s) | 307 |
| LmnI | GCTCC | 3 cut(s) | 53, 1265, 1302 |
| Lsp1109I | GCAGC | 4 cut(s) | 178, 599, 868, 1018 |
| LweI | GCATC | 3 cut(s) | 901, 1157, 1421 |
| MaeI | CTAG | 3 cut(s) | 114, 623, 1397 |
| MaeII | ACGT | 1 cut(s) | 450 |
| MaeIII | GTNAC | 4 cut(s) | 109, 301, 849, 1270 |
| MalI | GATC | 1 cut(s) | 309 |
| MboI | GATC | 1 cut(s) | 307 |
| MboII | GAAGA | 8 cut(s) | 224, 423, 510, 820, 920, 1017, 1318, 1412 |
| MfeI | CAATTG | 1 cut(s) | 264 |
| MhlI | GDGCHC | 4 cut(s) | 58, 134, 987, 1307 |
| MluCI | AATT | 8 cut(s) | 204, 264, 441, 618, 666, 860, 998, 1285 |
| MlyI | GAGTC | 2 cut(s) | 408, 653 |
| MmeI | TCCRAC | 1 cut(s) | 814 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MroXI | GAANNNNTTC | 1 cut(s) | 693 |
| MseI | TTAA | 4 cut(s) | 989, 1215, 1238, 1338 |
| MslI | CAYNNNNRTG | 3 cut(s) | 116, 137, 1118 |
| MspA1I | CMGCKG | 1 cut(s) | 191 |
| MspI | CCGG | 2 cut(s) | 165, 915 |
| MspR9I | CCNGG | 4 cut(s) | 574, 606, 1180, 1264 |
| MunI | CAATTG | 1 cut(s) | 264 |
| Mva1269I | GAATGC | 1 cut(s) | 394 |
| MvaI | CCWGG | 4 cut(s) | 574, 606, 1180, 1264 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 389, 429, 596 |
| NcoI | CCATGG | 2 cut(s) | 45, 582 |
| NdeII | GATC | 1 cut(s) | 307 |
| NlaIII | CATG | 6 cut(s) | 34, 49, 586, 754, 1135, 1168 |
| NlaIV | GGNNCC | 1 cut(s) | 456 |
| NmuCI | GTSAC | 1 cut(s) | 109 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 1135 |
| OliI | CACNNNNGTG | 2 cut(s) | 116, 137 |
| PasI | CCCWGGG | 1 cut(s) | 1179 |
| PctI | GAATGC | 1 cut(s) | 394 |
| PdmI | GAANNNNTTC | 1 cut(s) | 693 |
| PfeI | GAWTC | 3 cut(s) | 86, 197, 501 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 573 |
| PfoI | TCCNGGA | 1 cut(s) | 1262 |
| PkrI | GCNGC | 4 cut(s) | 193, 589, 858, 1033 |
| PleI | GAGTC | 2 cut(s) | 408, 652 |
| PpsI | GAGTC | 2 cut(s) | 408, 652 |
| Ppu21I | YACGTR | 1 cut(s) | 451 |
| PpuMI | RGGWCCY | 3 cut(s) | 487, 682, 1084 |
| Psp124BI | GAGCTC | 1 cut(s) | 1307 |
| Psp5II | RGGWCCY | 3 cut(s) | 487, 682, 1084 |
| Psp6I | CCWGG | 4 cut(s) | 572, 604, 1178, 1262 |
| PspGI | CCWGG | 4 cut(s) | 572, 604, 1178, 1262 |
| PspN4I | GGNNCC | 1 cut(s) | 456 |
| PspPI | GGNCC | 5 cut(s) | 142, 220, 487, 682, 1084 |
| PspPPI | RGGWCCY | 3 cut(s) | 487, 682, 1084 |
| PstI | CTGCAG | 2 cut(s) | 361, 858 |
| PstNI | CAGNNNCTG | 1 cut(s) | 194 |
| PvuII | CAGCTG | 1 cut(s) | 191 |
| RsaI | GTAC | 3 cut(s) | 355, 456, 464 |
| RsaNI | GTAC | 3 cut(s) | 354, 455, 463 |
| RseI | CAYNNNNRTG | 3 cut(s) | 116, 137, 1118 |
| SacI | GAGCTC | 1 cut(s) | 1307 |
| SaqAI | TTAA | 4 cut(s) | 989, 1215, 1238, 1338 |
| SatI | GCNGC | 4 cut(s) | 192, 588, 857, 1032 |
| Sau3AI | GATC | 1 cut(s) | 307 |
| Sau96I | GGNCC | 5 cut(s) | 142, 220, 487, 682, 1084 |
| ScaI | AGTACT | 1 cut(s) | 355 |
| SchI | GAGTC | 2 cut(s) | 408, 653 |
| ScrFI | CCNGG | 4 cut(s) | 574, 606, 1180, 1264 |
| SduI | GDGCHC | 4 cut(s) | 58, 134, 987, 1307 |
| SfaNI | GCATC | 3 cut(s) | 901, 1157, 1421 |
| SfcI | CTRYAG | 4 cut(s) | 174, 357, 372, 854 |
| SinI | GGWCC | 4 cut(s) | 220, 487, 682, 1084 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 116, 137, 1118 |
| SmlI | CTYRAG | 2 cut(s) | 418, 1045 |
| SmoI | CTYRAG | 2 cut(s) | 418, 1045 |
| SpeI | ACTAGT | 1 cut(s) | 113 |
| Sse9I | AATT | 8 cut(s) | 204, 264, 441, 618, 666, 860, 998, 1285 |
| SsiI | CCGC | 2 cut(s) | 105, 423 |
| SspI | AATATT | 1 cut(s) | 348 |
| SspMI | CTAG | 3 cut(s) | 114, 623, 1397 |
| SstI | GAGCTC | 1 cut(s) | 1307 |
| StyD4I | CCNGG | 4 cut(s) | 572, 604, 1178, 1262 |
| StyI | CCWWGG | 4 cut(s) | 45, 483, 582, 1383 |
| TaaI | ACNGT | 2 cut(s) | 849, 1343 |
| TaiI | ACGT | 1 cut(s) | 453 |
| TaqI | TCGA | 1 cut(s) | 78 |
| TaqII | GACCGA | 1 cut(s) | 305 |
| TasI | AATT | 8 cut(s) | 204, 264, 441, 618, 666, 860, 998, 1285 |
| TatI | WGTACW | 2 cut(s) | 353, 462 |
| TfiI | GAWTC | 3 cut(s) | 86, 197, 501 |
| Tru1I | TTAA | 4 cut(s) | 989, 1215, 1238, 1338 |
| Tru9I | TTAA | 4 cut(s) | 989, 1215, 1238, 1338 |
| TscAI | CASTG | 4 cut(s) | 465, 951, 1100, 1338 |
| TseFI | GTSAC | 1 cut(s) | 109 |
| TseI | GCWGC | 4 cut(s) | 191, 587, 856, 1031 |
| Tsp45I | GTSAC | 1 cut(s) | 109 |
| TspDTI | ATGAA | 5 cut(s) | 174, 273, 305, 544, 625 |
| TspGWI | ACGGA | 2 cut(s) | 906, 1007 |
| TspRI | CASTG | 4 cut(s) | 465, 951, 1100, 1338 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 573 |
| VpaK11BI | GGWCC | 4 cut(s) | 220, 487, 682, 1084 |
| XapI | RAATTY | 1 cut(s) | 618 |
| XbaI | TCTAGA | 1 cut(s) | 1396 |
| XceI | RCATGY | 1 cut(s) | 1135 |
| XmiI | GTMKAC | 3 cut(s) | 610, 673, 758 |
| XmnI | GAANNNNTTC | 1 cut(s) | 693 |
| XspI | CTAG | 3 cut(s) | 114, 623, 1397 |
| ZrmI | AGTACT | 1 cut(s) | 355 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |