Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 469 |
| AccB1I | GGYRCC | 2 cut(s) | 469, 884 |
| AccI | GTMKAC | 2 cut(s) | 337, 477 |
| AciI | CCGC | 2 cut(s) | 549, 1356 |
| AclI | AACGTT | 2 cut(s) | 1227, 1409 |
| AclWI | GGATC | 1 cut(s) | 510 |
| AcoI | YGGCCR | 1 cut(s) | 675 |
| AcsI | RAATTY | 2 cut(s) | 219, 1419 |
| AcuI | CTGAAG | 3 cut(s) | 60, 507, 698 |
| AdeI | CACNNNGTG | 1 cut(s) | 404 |
| AfaI | GTAC | 4 cut(s) | 343, 471, 630, 1330 |
| AfeI | AGCGCT | 1 cut(s) | 148 |
| AgsI | TTSAA | 4 cut(s) | 270, 704, 1300, 1424 |
| AjnI | CCWGG | 4 cut(s) | 103, 587, 979, 1193 |
| AluBI | AGCT | 7 cut(s) | 74, 101, 316, 527, 1007, 1241, 1320 |
| AluI | AGCT | 7 cut(s) | 74, 101, 316, 527, 1007, 1241, 1320 |
| Alw21I | GWGCWC | 3 cut(s) | 301, 1002, 1322 |
| Alw26I | GTCTC | 1 cut(s) | 666 |
| Alw44I | GTGCAC | 1 cut(s) | 297 |
| AlwI | GGATC | 1 cut(s) | 510 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 721, 1102 |
| Aor51HI | AGCGCT | 1 cut(s) | 148 |
| AoxI | GGCC | 2 cut(s) | 235, 675 |
| ApaLI | GTGCAC | 1 cut(s) | 297 |
| ApeKI | GCWGC | 4 cut(s) | 71, 371, 755, 790 |
| ApoI | RAATTY | 2 cut(s) | 219, 1419 |
| AseI | ATTAAT | 1 cut(s) | 842 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1419 |
| Asp718I | GGTACC | 1 cut(s) | 469 |
| AspLEI | GCGC | 1 cut(s) | 149 |
| AspS9I | GGNCC | 2 cut(s) | 235, 1099 |
| AsuC2I | CCSGG | 1 cut(s) | 376 |
| AsuHPI | GGTGA | 1 cut(s) | 92 |
| AvaII | GGWCC | 1 cut(s) | 1099 |
| BaeGI | GKGCMC | 1 cut(s) | 301 |
| BalI | TGGCCA | 1 cut(s) | 677 |
| BanI | GGYRCC | 2 cut(s) | 469, 884 |
| BanII | GRGCYC | 2 cut(s) | 861, 1322 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 18, 50 |
| BbsI | GAAGAC | 1 cut(s) | 1027 |
| Bbv12I | GWGCWC | 3 cut(s) | 301, 1002, 1322 |
| BbvCI | CCTCAGC | 1 cut(s) | 121 |
| BbvI | GCAGC | 4 cut(s) | 83, 358, 767, 802 |
| BccI | CCATC | 2 cut(s) | 628, 1303 |
| BciT130I | CCWGG | 4 cut(s) | 105, 589, 981, 1195 |
| BcnI | CCSGG | 1 cut(s) | 376 |
| BcoDI | GTCTC | 1 cut(s) | 666 |
| BfaI | CTAG | 2 cut(s) | 809, 1385 |
| BfmI | CTRYAG | 1 cut(s) | 189 |
| BfoI | RGCGCY | 1 cut(s) | 150 |
| BisI | GCNGC | 5 cut(s) | 72, 372, 550, 756, 791 |
| BlpI | GCTNAGC | 2 cut(s) | 553, 1321 |
| BlsI | GCNGC | 5 cut(s) | 73, 373, 551, 757, 792 |
| Bme1390I | CCNGG | 5 cut(s) | 105, 376, 589, 981, 1195 |
| Bme18I | GGWCC | 1 cut(s) | 1099 |
| BmgT120I | GGNCC | 2 cut(s) | 235, 1099 |
| BmiI | GGNNCC | 3 cut(s) | 169, 471, 886 |
| BmrFI | CCNGG | 5 cut(s) | 105, 376, 589, 981, 1195 |
| BmrI | ACTGGG | 1 cut(s) | 125 |
| BmsI | GCATC | 4 cut(s) | 102, 727, 916, 1436 |
| BmuI | ACTGGG | 1 cut(s) | 125 |
| BoxI | GACNNNNGTC | 1 cut(s) | 430 |
| BpiI | GAAGAC | 1 cut(s) | 1027 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 779, 811, 817, 849, 843, 875 |
| BpmI | CTGGAG | 1 cut(s) | 87 |
| Bpu10I | CCTNAGC | 1 cut(s) | 121 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 553, 1321 |
| BpuEI | CTTGAG | 2 cut(s) | 1012, 1081 |
| BpuMI | CCSGG | 1 cut(s) | 376 |
| BsaBI | GATNNNNATC | 1 cut(s) | 199 |
| BsaJI | CCNNGG | 7 cut(s) | 21, 498, 571, 597, 665, 1193, 1194 |
| Bse1I | ACTGG | 6 cut(s) | 131, 306, 564, 966, 1041, 1271 |
| Bse8I | GATNNNNATC | 1 cut(s) | 199 |
| BseBI | CCWGG | 4 cut(s) | 105, 589, 981, 1195 |
| BseDI | CCNNGG | 7 cut(s) | 21, 498, 571, 597, 665, 1193, 1194 |
| BseGI | GGATG | 1 cut(s) | 200 |
| BseJI | GATNNNNATC | 1 cut(s) | 199 |
| BseMII | CTCAG | 7 cut(s) | 135, 270, 497, 713, 761, 1335, 1359 |
| BseNI | ACTGG | 6 cut(s) | 131, 306, 564, 966, 1041, 1271 |
| BseSI | GKGCMC | 1 cut(s) | 301 |
| BseXI | GCAGC | 4 cut(s) | 83, 358, 767, 802 |
| BshFI | GGCC | 2 cut(s) | 237, 677 |
| BshNI | GGYRCC | 2 cut(s) | 469, 884 |
| BsiHKAI | GWGCWC | 3 cut(s) | 301, 1002, 1322 |
| BsiSI | CCGG | 2 cut(s) | 375, 1391 |
| BslFI | GGGAC | 2 cut(s) | 850, 964 |
| BsmAI | GTCTC | 1 cut(s) | 666 |
| BsmFI | GGGAC | 2 cut(s) | 850, 964 |
| BsmI | GAATGC | 1 cut(s) | 1085 |
| BsnI | GGCC | 2 cut(s) | 237, 677 |
| Bsp1286I | GDGCHC | 4 cut(s) | 301, 861, 1002, 1322 |
| Bsp1407I | TGTACA | 1 cut(s) | 628 |
| Bsp143I | GATC | 1 cut(s) | 502 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 553, 1321 |
| Bsp19I | CCATGG | 1 cut(s) | 21 |
| BspACI | CCGC | 2 cut(s) | 549, 1356 |
| BspANI | GGCC | 2 cut(s) | 237, 677 |
| BspCNI | CTCAG | 7 cut(s) | 134, 269, 498, 714, 760, 1334, 1358 |
| BspHI | TCATGA | 1 cut(s) | 1305 |
| BspLI | GGNNCC | 3 cut(s) | 169, 471, 886 |
| BspPI | GGATC | 1 cut(s) | 510 |
| BspQI | GCTCTTC | 1 cut(s) | 717 |
| BspT107I | GGYRCC | 2 cut(s) | 469, 884 |
| BsrGI | TGTACA | 1 cut(s) | 628 |
| BsrI | ACTGG | 6 cut(s) | 131, 306, 564, 966, 1041, 1271 |
| BssECI | CCNNGG | 7 cut(s) | 21, 498, 571, 597, 665, 1193, 1194 |
| BssMI | GATC | 1 cut(s) | 502 |
| BssNAI | GTATAC | 1 cut(s) | 478 |
| BssT1I | CCWWGG | 5 cut(s) | 21, 498, 571, 597, 665 |
| Bst1107I | GTATAC | 1 cut(s) | 478 |
| Bst2UI | CCWGG | 4 cut(s) | 105, 589, 981, 1195 |
| Bst4CI | ACNGT | 4 cut(s) | 244, 429, 580, 1366 |
| Bst6I | CTCTTC | 1 cut(s) | 717 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 404, 1433 |
| BstAUI | TGTACA | 1 cut(s) | 628 |
| BstC8I | GCNNGC | 1 cut(s) | 675 |
| BstDSI | CCRYGG | 1 cut(s) | 21 |
| BstF5I | GGATG | 1 cut(s) | 200 |
| BstH2I | RGCGCY | 1 cut(s) | 150 |
| BstHHI | GCGC | 1 cut(s) | 149 |
| BstKTI | GATC | 1 cut(s) | 505 |
| BstMAI | GTCTC | 1 cut(s) | 666 |
| BstMBI | GATC | 1 cut(s) | 502 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 155, 404, 1433 |
| BstNI | CCWGG | 4 cut(s) | 105, 589, 981, 1195 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 430 |
| BstSCI | CCNGG | 5 cut(s) | 103, 374, 587, 979, 1193 |
| BstSFI | CTRYAG | 1 cut(s) | 189 |
| BstSLI | GKGCMC | 1 cut(s) | 301 |
| BstV1I | GCAGC | 4 cut(s) | 83, 358, 767, 802 |
| BstV2I | GAAGAC | 1 cut(s) | 1027 |
| BstX2I | RGATCY | 1 cut(s) | 502 |
| BstYI | RGATCY | 1 cut(s) | 502 |
| BstZ17I | GTATAC | 1 cut(s) | 478 |
| BsuRI | GGCC | 2 cut(s) | 237, 677 |
| BtgI | CCRYGG | 1 cut(s) | 21 |
| BtsCI | GGATG | 1 cut(s) | 200 |
| BtsI | GCAGTG | 1 cut(s) | 94 |
| BtsIMutI | CAGTG | 4 cut(s) | 94, 299, 557, 959 |
| Cac8I | GCNNGC | 1 cut(s) | 675 |
| CaiI | CAGNNNCTG | 2 cut(s) | 721, 1102 |
| CciI | TCATGA | 1 cut(s) | 1305 |
| CfoI | GCGC | 1 cut(s) | 149 |
| Cfr13I | GGNCC | 2 cut(s) | 235, 1099 |
| Csp6I | GTAC | 4 cut(s) | 342, 470, 629, 1329 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 115, 150 |
| CviAII | CATG | 6 cut(s) | 22, 766, 862, 1180, 1204, 1306 |
| CviQI | GTAC | 4 cut(s) | 342, 470, 629, 1329 |
| DpnI | GATC | 1 cut(s) | 504 |
| DpnII | GATC | 1 cut(s) | 502 |
| DraIII | CACNNNGTG | 1 cut(s) | 404 |
| EaeI | YGGCCR | 1 cut(s) | 675 |
| Eam1104I | CTCTTC | 1 cut(s) | 717 |
| EarI | CTCTTC | 1 cut(s) | 717 |
| Ecl136II | GAGCTC | 1 cut(s) | 1320 |
| Eco130I | CCWWGG | 5 cut(s) | 21, 498, 571, 597, 665 |
| Eco24I | GRGCYC | 2 cut(s) | 861, 1322 |
| Eco47I | GGWCC | 1 cut(s) | 1099 |
| Eco47III | AGCGCT | 1 cut(s) | 148 |
| Eco53kI | GAGCTC | 1 cut(s) | 1320 |
| Eco57I | CTGAAG | 3 cut(s) | 60, 507, 698 |
| EcoICRI | GAGCTC | 1 cut(s) | 1320 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1099 |
| EcoRII | CCWGG | 4 cut(s) | 103, 587, 979, 1193 |
| EcoT14I | CCWWGG | 5 cut(s) | 21, 498, 571, 597, 665 |
| EcoT38I | GRGCYC | 2 cut(s) | 861, 1322 |
| ErhI | CCWWGG | 5 cut(s) | 21, 498, 571, 597, 665 |
| FaeI | CATG | 6 cut(s) | 25, 769, 865, 1183, 1207, 1309 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 505, 537, 696, 728 |
| FaqI | GGGAC | 2 cut(s) | 850, 964 |
| FatI | CATG | 6 cut(s) | 21, 765, 861, 1179, 1203, 1305 |
| FblI | GTMKAC | 2 cut(s) | 337, 477 |
| Fnu4HI | GCNGC | 5 cut(s) | 72, 372, 550, 756, 791 |
| FokI | GGATG | 1 cut(s) | 187 |
| FriOI | GRGCYC | 2 cut(s) | 861, 1322 |
| Fsp4HI | GCNGC | 5 cut(s) | 72, 372, 550, 756, 791 |
| FspBI | CTAG | 2 cut(s) | 809, 1385 |
| GlaI | GCGC | 1 cut(s) | 148 |
| GluI | GCNGC | 5 cut(s) | 72, 372, 550, 756, 791 |
| GsuI | CTGGAG | 1 cut(s) | 87 |
| HaeII | RGCGCY | 1 cut(s) | 150 |
| HaeIII | GGCC | 2 cut(s) | 237, 677 |
| HapII | CCGG | 2 cut(s) | 375, 1391 |
| HhaI | GCGC | 1 cut(s) | 149 |
| Hin1II | CATG | 6 cut(s) | 25, 769, 865, 1183, 1207, 1309 |
| Hin6I | GCGC | 1 cut(s) | 147 |
| HinP1I | GCGC | 1 cut(s) | 147 |
| HinfI | GANTC | 5 cut(s) | 212, 659, 779, 804, 976 |
| HpaII | CCGG | 2 cut(s) | 375, 1391 |
| HphI | GGTGA | 1 cut(s) | 92 |
| Hpy166II | GTNNAC | 8 cut(s) | 299, 307, 338, 384, 478, 519, 631, 1264 |
| Hpy188I | TCNGA | 4 cut(s) | 507, 723, 799, 1210 |
| Hpy188III | TCNNGA | 5 cut(s) | 216, 656, 749, 1097, 1306 |
| Hpy8I | GTNNAC | 8 cut(s) | 299, 307, 338, 384, 478, 519, 631, 1264 |
| HpyAV | CCTTC | 3 cut(s) | 23, 507, 1306 |
| HpyCH4III | ACNGT | 4 cut(s) | 244, 429, 580, 1366 |
| HpyCH4IV | ACGT | 3 cut(s) | 340, 1227, 1409 |
| HpyCH4V | TGCA | 8 cut(s) | 71, 87, 248, 299, 398, 871, 908, 929 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 155, 404, 1433 |
| HpySE526I | ACGT | 3 cut(s) | 340, 1227, 1409 |
| Hsp92II | CATG | 6 cut(s) | 25, 769, 865, 1183, 1207, 1309 |
| HspAI | GCGC | 1 cut(s) | 147 |
| KpnI | GGTACC | 1 cut(s) | 473 |
| Kzo9I | GATC | 1 cut(s) | 502 |
| LguI | GCTCTTC | 1 cut(s) | 717 |
| LmnI | GCTCC | 2 cut(s) | 106, 1317 |
| Lsp1109I | GCAGC | 4 cut(s) | 83, 358, 767, 802 |
| LweI | GCATC | 4 cut(s) | 102, 727, 916, 1436 |
| MaeI | CTAG | 2 cut(s) | 809, 1385 |
| MaeII | ACGT | 3 cut(s) | 340, 1227, 1409 |
| MaeIII | GTNAC | 3 cut(s) | 33, 1120, 1285 |
| MalI | GATC | 1 cut(s) | 504 |
| MboI | GATC | 1 cut(s) | 502 |
| MboII | GAAGA | 8 cut(s) | 18, 38, 239, 640, 704, 1032, 1333, 1427 |
| MfeI | CAATTG | 2 cut(s) | 279, 1431 |
| MflI | RGATCY | 1 cut(s) | 502 |
| MhlI | GDGCHC | 4 cut(s) | 301, 861, 1002, 1322 |
| MlsI | TGGCCA | 1 cut(s) | 677 |
| MluNI | TGGCCA | 1 cut(s) | 677 |
| MlyI | GAGTC | 2 cut(s) | 668, 813 |
| MmeI | TCCRAC | 2 cut(s) | 829, 997 |
| MnlI | CCTC | 6 cut(s) | 130, 214, 278, 503, 719, 1111 |
| Mox20I | TGGCCA | 1 cut(s) | 677 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1419 |
| MscI | TGGCCA | 1 cut(s) | 677 |
| MseI | TTAA | 3 cut(s) | 842, 1230, 1361 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1133 |
| Msp20I | TGGCCA | 1 cut(s) | 677 |
| MspA1I | CMGCKG | 1 cut(s) | 1241 |
| MspI | CCGG | 2 cut(s) | 375, 1391 |
| MspR9I | CCNGG | 5 cut(s) | 105, 376, 589, 981, 1195 |
| MunI | CAATTG | 2 cut(s) | 279, 1431 |
| Mva1269I | GAATGC | 1 cut(s) | 1085 |
| MvaI | CCWGG | 4 cut(s) | 105, 589, 981, 1195 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 155, 404, 1433 |
| NciI | CCSGG | 1 cut(s) | 376 |
| NcoI | CCATGG | 1 cut(s) | 21 |
| NdeII | GATC | 1 cut(s) | 502 |
| NlaIII | CATG | 6 cut(s) | 25, 769, 865, 1183, 1207, 1309 |
| NlaIV | GGNNCC | 3 cut(s) | 169, 471, 886 |
| NmuCI | GTSAC | 1 cut(s) | 1120 |
| PagI | TCATGA | 1 cut(s) | 1305 |
| PasI | CCCWGGG | 1 cut(s) | 1194 |
| PciSI | GCTCTTC | 1 cut(s) | 717 |
| PctI | GAATGC | 1 cut(s) | 1085 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1419 |
| PfeI | GAWTC | 3 cut(s) | 212, 779, 976 |
| PkrI | GCNGC | 5 cut(s) | 73, 373, 551, 757, 792 |
| PleI | GAGTC | 2 cut(s) | 667, 812 |
| PpsI | GAGTC | 2 cut(s) | 667, 812 |
| PpuMI | RGGWCCY | 1 cut(s) | 1099 |
| PshAI | GACNNNNGTC | 1 cut(s) | 430 |
| PshBI | ATTAAT | 1 cut(s) | 842 |
| Psp124BI | GAGCTC | 1 cut(s) | 1322 |
| Psp1406I | AACGTT | 2 cut(s) | 1227, 1409 |
| Psp5II | RGGWCCY | 1 cut(s) | 1099 |
| Psp6I | CCWGG | 4 cut(s) | 103, 587, 979, 1193 |
| PspGI | CCWGG | 4 cut(s) | 103, 587, 979, 1193 |
| PspN4I | GGNNCC | 3 cut(s) | 169, 471, 886 |
| PspPI | GGNCC | 2 cut(s) | 235, 1099 |
| PspPPI | RGGWCCY | 1 cut(s) | 1099 |
| PstNI | CAGNNNCTG | 2 cut(s) | 721, 1102 |
| PsuI | RGATCY | 1 cut(s) | 502 |
| PvuII | CAGCTG | 1 cut(s) | 1241 |
| RsaI | GTAC | 4 cut(s) | 343, 471, 630, 1330 |
| RsaNI | GTAC | 4 cut(s) | 342, 470, 629, 1329 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1133 |
| SacI | GAGCTC | 1 cut(s) | 1322 |
| SapI | GCTCTTC | 1 cut(s) | 717 |
| SaqAI | TTAA | 3 cut(s) | 842, 1230, 1361 |
| SatI | GCNGC | 5 cut(s) | 72, 372, 550, 756, 791 |
| Sau3AI | GATC | 1 cut(s) | 502 |
| Sau96I | GGNCC | 2 cut(s) | 235, 1099 |
| SchI | GAGTC | 2 cut(s) | 668, 813 |
| ScrFI | CCNGG | 5 cut(s) | 105, 376, 589, 981, 1195 |
| SduI | GDGCHC | 4 cut(s) | 301, 861, 1002, 1322 |
| SfaNI | GCATC | 4 cut(s) | 102, 727, 916, 1436 |
| SfcI | CTRYAG | 1 cut(s) | 189 |
| SinI | GGWCC | 1 cut(s) | 1099 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1133 |
| SmlI | CTYRAG | 2 cut(s) | 991, 1060 |
| SmoI | CTYRAG | 2 cut(s) | 991, 1060 |
| SsiI | CCGC | 2 cut(s) | 549, 1356 |
| SspMI | CTAG | 2 cut(s) | 809, 1385 |
| SstI | GAGCTC | 1 cut(s) | 1322 |
| StyD4I | CCNGG | 5 cut(s) | 103, 374, 587, 979, 1193 |
| StyI | CCWWGG | 5 cut(s) | 21, 498, 571, 597, 665 |
| TaaI | ACNGT | 4 cut(s) | 244, 429, 580, 1366 |
| TaiI | ACGT | 3 cut(s) | 343, 1230, 1412 |
| TatI | WGTACW | 2 cut(s) | 628, 1328 |
| TauI | GCSGC | 1 cut(s) | 552 |
| TfiI | GAWTC | 3 cut(s) | 212, 779, 976 |
| Tru1I | TTAA | 3 cut(s) | 842, 1230, 1361 |
| Tru9I | TTAA | 3 cut(s) | 842, 1230, 1361 |
| TscAI | CASTG | 4 cut(s) | 94, 306, 564, 966 |
| TseFI | GTSAC | 1 cut(s) | 1120 |
| TseI | GCWGC | 4 cut(s) | 71, 371, 755, 790 |
| Tsp45I | GTSAC | 1 cut(s) | 1120 |
| TspDTI | ATGAA | 7 cut(s) | 189, 316, 640, 1129, 1155, 1294, 1363 |
| TspRI | CASTG | 4 cut(s) | 94, 306, 564, 966 |
| VneI | GTGCAC | 1 cut(s) | 297 |
| VpaK11BI | GGWCC | 1 cut(s) | 1099 |
| VspI | ATTAAT | 1 cut(s) | 842 |
| XapI | RAATTY | 2 cut(s) | 219, 1419 |
| XmiI | GTMKAC | 2 cut(s) | 337, 477 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1419 |
| XspI | CTAG | 2 cut(s) | 809, 1385 |