Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 761, 1157 |
| Acc65I | GGTACC | 2 cut(s) | 403, 725 |
| AccB1I | GGYRCC | 3 cut(s) | 345, 403, 725 |
| AciI | CCGC | 3 cut(s) | 483, 526, 891 |
| AclI | AACGTT | 1 cut(s) | 1068 |
| AclWI | GGATC | 1 cut(s) | 264 |
| AcuI | CTGAAG | 2 cut(s) | 54, 441 |
| AcyI | GRCGYC | 1 cut(s) | 381 |
| AfaI | GTAC | 6 cut(s) | 277, 405, 413, 564, 727, 980 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 955 |
| AgsI | TTSAA | 4 cut(s) | 204, 780, 1141, 1189 |
| AjnI | CCWGG | 5 cut(s) | 348, 521, 648, 820, 1034 |
| AluBI | AGCT | 6 cut(s) | 35, 68, 461, 933, 1082, 1161 |
| AluI | AGCT | 6 cut(s) | 35, 68, 461, 933, 1082, 1161 |
| Alw21I | GWGCWC | 4 cut(s) | 92, 843, 902, 1163 |
| Alw26I | GTCTC | 1 cut(s) | 600 |
| AlwI | GGATC | 1 cut(s) | 264 |
| Ama87I | CYCGRG | 1 cut(s) | 901 |
| AoxI | GGCC | 1 cut(s) | 178 |
| ApeKI | GCWGC | 2 cut(s) | 305, 631 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 624 |
| Asp718I | GGTACC | 2 cut(s) | 403, 725 |
| AspA2I | CCTAGG | 1 cut(s) | 505 |
| AspS9I | GGNCC | 2 cut(s) | 178, 528 |
| AsuHPI | GGTGA | 2 cut(s) | 1114, 1126 |
| AvaI | CYCGRG | 1 cut(s) | 901 |
| AvaII | GGWCC | 1 cut(s) | 528 |
| AvrII | CCTAGG | 1 cut(s) | 505 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 395, 428 |
| BanI | GGYRCC | 3 cut(s) | 345, 403, 725 |
| BanII | GRGCYC | 2 cut(s) | 702, 1163 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 12, 44 |
| BbsI | GAAGAC | 2 cut(s) | 177, 868 |
| Bbv12I | GWGCWC | 4 cut(s) | 92, 843, 902, 1163 |
| BbvI | GCAGC | 2 cut(s) | 292, 643 |
| BccI | CCATC | 2 cut(s) | 562, 1144 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 79, 113 |
| BciT130I | CCWGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| BciVI | GTATCC | 2 cut(s) | 131, 810 |
| BcoDI | GTCTC | 1 cut(s) | 600 |
| BfaI | CTAG | 2 cut(s) | 32, 506 |
| BfmI | CTRYAG | 3 cut(s) | 132, 306, 321 |
| BfuAI | ACCTGC | 2 cut(s) | 761, 1157 |
| BfuI | GTATCC | 2 cut(s) | 131, 810 |
| BisI | GCNGC | 2 cut(s) | 306, 632 |
| BlnI | CCTAGG | 1 cut(s) | 505 |
| BlpI | GCTNAGC | 1 cut(s) | 1162 |
| BlsI | GCNGC | 2 cut(s) | 307, 633 |
| Bme1390I | CCNGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| Bme18I | GGWCC | 1 cut(s) | 528 |
| BmeT110I | CYCGRG | 1 cut(s) | 901 |
| BmgT120I | GGNCC | 2 cut(s) | 178, 528 |
| BmiI | GGNNCC | 4 cut(s) | 112, 347, 405, 727 |
| BmrFI | CCNGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| BmsI | GCATC | 2 cut(s) | 462, 757 |
| BoxI | GACNNNNGTC | 1 cut(s) | 364 |
| BpiI | GAAGAC | 2 cut(s) | 177, 868 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 658, 690, 684, 716 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1162 |
| BsaAI | YACGTR | 1 cut(s) | 400 |
| BsaBI | GATNNNNATC | 1 cut(s) | 142 |
| BsaHI | GRCGYC | 1 cut(s) | 381 |
| BsaJI | CCNNGG | 6 cut(s) | 15, 505, 531, 599, 1034, 1035 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 955 |
| Bse1I | ACTGG | 1 cut(s) | 1112 |
| Bse8I | GATNNNNATC | 1 cut(s) | 142 |
| BseBI | CCWGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| BseDI | CCNNGG | 6 cut(s) | 15, 505, 531, 599, 1034, 1035 |
| BseJI | GATNNNNATC | 1 cut(s) | 142 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 955 |
| BseMII | CTCAG | 4 cut(s) | 77, 479, 1085, 1176 |
| BseNI | ACTGG | 1 cut(s) | 1112 |
| BseXI | GCAGC | 2 cut(s) | 292, 643 |
| BshFI | GGCC | 1 cut(s) | 180 |
| BshNI | GGYRCC | 3 cut(s) | 345, 403, 725 |
| BsiHKAI | GWGCWC | 4 cut(s) | 92, 843, 902, 1163 |
| BsiHKCI | CYCGRG | 1 cut(s) | 901 |
| BslFI | GGGAC | 2 cut(s) | 259, 805 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 955 |
| BsmAI | GTCTC | 1 cut(s) | 600 |
| BsmFI | GGGAC | 2 cut(s) | 259, 805 |
| BsmI | GAATGC | 2 cut(s) | 343, 926 |
| BsnI | GGCC | 1 cut(s) | 180 |
| BsoBI | CYCGRG | 1 cut(s) | 901 |
| Bsp1286I | GDGCHC | 5 cut(s) | 92, 702, 843, 902, 1163 |
| Bsp1407I | TGTACA | 3 cut(s) | 411, 562, 978 |
| Bsp143I | GATC | 2 cut(s) | 256, 436 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1162 |
| Bsp19I | CCATGG | 1 cut(s) | 15 |
| BspACI | CCGC | 3 cut(s) | 483, 526, 891 |
| BspANI | GGCC | 1 cut(s) | 180 |
| BspCNI | CTCAG | 4 cut(s) | 78, 478, 1086, 1175 |
| BspLI | GGNNCC | 4 cut(s) | 112, 347, 405, 727 |
| BspMAI | CTGCAG | 1 cut(s) | 310 |
| BspMI | ACCTGC | 2 cut(s) | 761, 1157 |
| BspPI | GGATC | 1 cut(s) | 264 |
| BspT107I | GGYRCC | 3 cut(s) | 345, 403, 725 |
| BsrGI | TGTACA | 3 cut(s) | 411, 562, 978 |
| BsrI | ACTGG | 1 cut(s) | 1112 |
| BssECI | CCNNGG | 6 cut(s) | 15, 505, 531, 599, 1034, 1035 |
| BssMI | GATC | 2 cut(s) | 256, 436 |
| BssNI | GRCGYC | 1 cut(s) | 381 |
| BssT1I | CCWWGG | 4 cut(s) | 15, 505, 531, 599 |
| Bst2UI | CCWGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| Bst4CI | ACNGT | 1 cut(s) | 363 |
| Bst6I | CTCTTC | 1 cut(s) | 15 |
| BstACI | GRCGYC | 1 cut(s) | 381 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 338, 746 |
| BstAUI | TGTACA | 3 cut(s) | 411, 562, 978 |
| BstBAI | YACGTR | 1 cut(s) | 400 |
| BstC8I | GCNNGC | 2 cut(s) | 889, 1091 |
| BstDEI | CTNAG | 5 cut(s) | 86, 440, 465, 1094, 1162 |
| BstDSI | CCRYGG | 1 cut(s) | 15 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 953 |
| BstKTI | GATC | 2 cut(s) | 259, 439 |
| BstMAI | GTCTC | 1 cut(s) | 600 |
| BstMBI | GATC | 2 cut(s) | 256, 436 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 338, 746, 897 |
| BstNI | CCWGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 364 |
| BstSCI | CCNGG | 5 cut(s) | 348, 521, 648, 820, 1034 |
| BstSFI | CTRYAG | 3 cut(s) | 132, 306, 321 |
| BstV1I | GCAGC | 2 cut(s) | 292, 643 |
| BstV2I | GAAGAC | 2 cut(s) | 177, 868 |
| BstX2I | RGATCY | 1 cut(s) | 256 |
| BstYI | RGATCY | 1 cut(s) | 256 |
| BsuI | GTATCC | 2 cut(s) | 131, 810 |
| BsuRI | GGCC | 1 cut(s) | 180 |
| BtgI | CCRYGG | 1 cut(s) | 15 |
| BtsI | GCAGTG | 1 cut(s) | 707 |
| BtsIMutI | CAGTG | 4 cut(s) | 67, 307, 375, 707 |
| BveI | ACCTGC | 2 cut(s) | 761, 1157 |
| Cac8I | GCNNGC | 2 cut(s) | 889, 1091 |
| Cfr13I | GGNCC | 2 cut(s) | 178, 528 |
| CseI | GACGC | 1 cut(s) | 370 |
| CsiI | ACCWGGT | 1 cut(s) | 820 |
| Csp6I | GTAC | 6 cut(s) | 276, 404, 412, 563, 726, 979 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 43, 78 |
| CviAII | CATG | 4 cut(s) | 16, 703, 1021, 1147 |
| CviQI | GTAC | 6 cut(s) | 276, 404, 412, 563, 726, 979 |
| DdeI | CTNAG | 5 cut(s) | 86, 440, 465, 1094, 1162 |
| DpnI | GATC | 2 cut(s) | 258, 438 |
| DpnII | GATC | 2 cut(s) | 256, 436 |
| Eam1104I | CTCTTC | 1 cut(s) | 15 |
| EarI | CTCTTC | 1 cut(s) | 15 |
| EciI | GGCGGA | 2 cut(s) | 541, 906 |
| Ecl136II | GAGCTC | 1 cut(s) | 1161 |
| Eco130I | CCWWGG | 4 cut(s) | 15, 505, 531, 599 |
| Eco24I | GRGCYC | 2 cut(s) | 702, 1163 |
| Eco47I | GGWCC | 1 cut(s) | 528 |
| Eco53kI | GAGCTC | 1 cut(s) | 1161 |
| Eco57I | CTGAAG | 2 cut(s) | 54, 441 |
| Eco88I | CYCGRG | 1 cut(s) | 901 |
| EcoICRI | GAGCTC | 1 cut(s) | 1161 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 953 |
| EcoRII | CCWGG | 5 cut(s) | 348, 521, 648, 820, 1034 |
| EcoT14I | CCWWGG | 4 cut(s) | 15, 505, 531, 599 |
| EcoT38I | GRGCYC | 2 cut(s) | 702, 1163 |
| ErhI | CCWWGG | 4 cut(s) | 15, 505, 531, 599 |
| FaeI | CATG | 4 cut(s) | 19, 706, 1024, 1150 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 439, 471 |
| FaqI | GGGAC | 2 cut(s) | 259, 805 |
| FatI | CATG | 4 cut(s) | 15, 702, 1020, 1146 |
| Fnu4HI | GCNGC | 2 cut(s) | 306, 632 |
| FriOI | GRGCYC | 2 cut(s) | 702, 1163 |
| Fsp4HI | GCNGC | 2 cut(s) | 306, 632 |
| FspBI | CTAG | 2 cut(s) | 32, 506 |
| GluI | GCNGC | 2 cut(s) | 306, 632 |
| HaeIII | GGCC | 1 cut(s) | 180 |
| HgaI | GACGC | 1 cut(s) | 370 |
| Hin1I | GRCGYC | 1 cut(s) | 381 |
| Hin1II | CATG | 4 cut(s) | 19, 706, 1024, 1150 |
| HinfI | GANTC | 3 cut(s) | 155, 593, 645 |
| HphI | GGTGA | 2 cut(s) | 1114, 1126 |
| Hpy166II | GTNNAC | 5 cut(s) | 412, 453, 565, 979, 1105 |
| Hpy188I | TCNGA | 5 cut(s) | 87, 292, 640, 860, 1051 |
| Hpy188III | TCNNGA | 3 cut(s) | 159, 590, 938 |
| Hpy8I | GTNNAC | 5 cut(s) | 412, 453, 565, 979, 1105 |
| Hpy99I | CGWCG | 1 cut(s) | 386 |
| HpyAV | CCTTC | 4 cut(s) | 124, 441, 949, 1147 |
| HpyCH4III | ACNGT | 1 cut(s) | 363 |
| HpyCH4IV | ACGT | 2 cut(s) | 399, 1068 |
| HpyCH4V | TGCA | 7 cut(s) | 233, 308, 332, 712, 734, 749, 770 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 338, 746, 897 |
| HpyF3I | CTNAG | 5 cut(s) | 86, 440, 465, 1094, 1162 |
| HpySE526I | ACGT | 2 cut(s) | 399, 1068 |
| Hsp92I | GRCGYC | 1 cut(s) | 381 |
| Hsp92II | CATG | 4 cut(s) | 19, 706, 1024, 1150 |
| KpnI | GGTACC | 2 cut(s) | 407, 729 |
| Kzo9I | GATC | 2 cut(s) | 256, 436 |
| LmnI | GCTCC | 2 cut(s) | 65, 1158 |
| Lsp1109I | GCAGC | 2 cut(s) | 292, 643 |
| LweI | GCATC | 2 cut(s) | 462, 757 |
| MabI | ACCWGGT | 1 cut(s) | 820 |
| MaeI | CTAG | 2 cut(s) | 32, 506 |
| MaeII | ACGT | 2 cut(s) | 399, 1068 |
| MaeIII | GTNAC | 4 cut(s) | 705, 826, 961, 1126 |
| MalI | GATC | 2 cut(s) | 258, 438 |
| MboI | GATC | 2 cut(s) | 256, 436 |
| MboII | GAAGA | 4 cut(s) | 32, 182, 873, 1177 |
| MflI | RGATCY | 1 cut(s) | 256 |
| MhlI | GDGCHC | 5 cut(s) | 92, 702, 843, 902, 1163 |
| MluCI | AATT | 5 cut(s) | 162, 192, 716, 1141, 1189 |
| MlyI | GAGTC | 2 cut(s) | 602, 654 |
| MmeI | TCCRAC | 1 cut(s) | 838 |
| MnlI | CCTC | 5 cut(s) | 16, 212, 266, 437, 952 |
| MroXI | GAANNNNTTC | 1 cut(s) | 624 |
| MseI | TTAA | 2 cut(s) | 845, 1071 |
| MslI | CAYNNNNRTG | 1 cut(s) | 974 |
| MspA1I | CMGCKG | 1 cut(s) | 1082 |
| MspR9I | CCNGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| Mva1269I | GAATGC | 2 cut(s) | 343, 926 |
| MvaI | CCWGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 338, 746, 897 |
| NcoI | CCATGG | 1 cut(s) | 15 |
| NdeII | GATC | 2 cut(s) | 256, 436 |
| NlaIII | CATG | 4 cut(s) | 19, 706, 1024, 1150 |
| NlaIV | GGNNCC | 4 cut(s) | 112, 347, 405, 727 |
| NmuCI | GTSAC | 2 cut(s) | 705, 961 |
| PaeR7I | CTCGAG | 1 cut(s) | 901 |
| PasI | CCCWGGG | 1 cut(s) | 1035 |
| PctI | GAATGC | 2 cut(s) | 343, 926 |
| PdmI | GAANNNNTTC | 1 cut(s) | 624 |
| PfeI | GAWTC | 1 cut(s) | 155 |
| PflFI | GACNNNGTC | 2 cut(s) | 644, 854 |
| PfoI | TCCNGGA | 1 cut(s) | 648 |
| PkrI | GCNGC | 2 cut(s) | 307, 633 |
| PleI | GAGTC | 2 cut(s) | 601, 653 |
| PpsI | GAGTC | 2 cut(s) | 601, 653 |
| Ppu21I | YACGTR | 1 cut(s) | 400 |
| PshAI | GACNNNNGTC | 1 cut(s) | 364 |
| Psp124BI | GAGCTC | 1 cut(s) | 1163 |
| Psp1406I | AACGTT | 1 cut(s) | 1068 |
| Psp6I | CCWGG | 5 cut(s) | 348, 521, 648, 820, 1034 |
| PspGI | CCWGG | 5 cut(s) | 348, 521, 648, 820, 1034 |
| PspN4I | GGNNCC | 4 cut(s) | 112, 347, 405, 727 |
| PspPI | GGNCC | 2 cut(s) | 178, 528 |
| PspXI | VCTCGAGB | 1 cut(s) | 901 |
| PstI | CTGCAG | 1 cut(s) | 310 |
| PsuI | RGATCY | 1 cut(s) | 256 |
| PsyI | GACNNNGTC | 2 cut(s) | 644, 854 |
| PvuII | CAGCTG | 1 cut(s) | 1082 |
| RsaI | GTAC | 6 cut(s) | 277, 405, 413, 564, 727, 980 |
| RsaNI | GTAC | 6 cut(s) | 276, 404, 412, 563, 726, 979 |
| RseI | CAYNNNNRTG | 1 cut(s) | 974 |
| SacI | GAGCTC | 1 cut(s) | 1163 |
| SaqAI | TTAA | 2 cut(s) | 845, 1071 |
| SatI | GCNGC | 2 cut(s) | 306, 632 |
| Sau3AI | GATC | 2 cut(s) | 256, 436 |
| Sau96I | GGNCC | 2 cut(s) | 178, 528 |
| SchI | GAGTC | 2 cut(s) | 602, 654 |
| ScrFI | CCNGG | 5 cut(s) | 350, 523, 650, 822, 1036 |
| SduI | GDGCHC | 5 cut(s) | 92, 702, 843, 902, 1163 |
| SexAI | ACCWGGT | 1 cut(s) | 820 |
| SfaNI | GCATC | 2 cut(s) | 462, 757 |
| SfcI | CTRYAG | 3 cut(s) | 132, 306, 321 |
| Sfr274I | CTCGAG | 1 cut(s) | 901 |
| SinI | GGWCC | 1 cut(s) | 528 |
| SlaI | CTCGAG | 1 cut(s) | 901 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 974 |
| SmlI | CTYRAG | 1 cut(s) | 901 |
| SmoI | CTYRAG | 1 cut(s) | 901 |
| Sse9I | AATT | 5 cut(s) | 162, 192, 716, 1141, 1189 |
| SsiI | CCGC | 3 cut(s) | 483, 526, 891 |
| SspI | AATATT | 1 cut(s) | 297 |
| SspMI | CTAG | 2 cut(s) | 32, 506 |
| SstI | GAGCTC | 1 cut(s) | 1163 |
| StyD4I | CCNGG | 5 cut(s) | 348, 521, 648, 820, 1034 |
| StyI | CCWWGG | 4 cut(s) | 15, 505, 531, 599 |
| TaaI | ACNGT | 1 cut(s) | 363 |
| TaiI | ACGT | 2 cut(s) | 402, 1071 |
| TaqI | TCGA | 2 cut(s) | 833, 902 |
| TasI | AATT | 5 cut(s) | 162, 192, 716, 1141, 1189 |
| TatI | WGTACW | 3 cut(s) | 411, 562, 978 |
| TfiI | GAWTC | 1 cut(s) | 155 |
| Tru1I | TTAA | 2 cut(s) | 845, 1071 |
| Tru9I | TTAA | 2 cut(s) | 845, 1071 |
| TscAI | CASTG | 4 cut(s) | 67, 307, 375, 714 |
| TseFI | GTSAC | 2 cut(s) | 705, 961 |
| TseI | GCWGC | 2 cut(s) | 305, 631 |
| Tsp45I | GTSAC | 2 cut(s) | 705, 961 |
| TspDTI | ATGAA | 1 cut(s) | 574 |
| TspGWI | ACGGA | 1 cut(s) | 269 |
| TspRI | CASTG | 4 cut(s) | 67, 307, 375, 714 |
| Tth111I | GACNNNGTC | 2 cut(s) | 644, 854 |
| VpaK11BI | GGWCC | 1 cut(s) | 528 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 953 |
| XhoI | CTCGAG | 1 cut(s) | 901 |
| XmaJI | CCTAGG | 1 cut(s) | 505 |
| XmnI | GAANNNNTTC | 1 cut(s) | 624 |
| XspI | CTAG | 2 cut(s) | 32, 506 |