Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1181 |
| AatII | GACGTC | 1 cut(s) | 75 |
| Acc36I | ACCTGC | 1 cut(s) | 899 |
| Acc65I | GGTACC | 1 cut(s) | 448 |
| AccB1I | GGYRCC | 2 cut(s) | 448, 863 |
| AccI | GTMKAC | 2 cut(s) | 456, 667 |
| AccII | CGCG | 1 cut(s) | 431 |
| AciI | CCGC | 1 cut(s) | 1335 |
| AclI | AACGTT | 1 cut(s) | 1206 |
| AclWI | GGATC | 2 cut(s) | 309, 489 |
| AcoI | YGGCCR | 3 cut(s) | 102, 579, 654 |
| AcsI | RAATTY | 1 cut(s) | 198 |
| AcuI | CTGAAG | 2 cut(s) | 486, 677 |
| AcyI | GRCGYC | 1 cut(s) | 72 |
| AdeI | CACNNNGTG | 1 cut(s) | 383 |
| AfaI | GTAC | 3 cut(s) | 322, 450, 609 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1257, 1335 |
| AgsI | TTSAA | 4 cut(s) | 57, 249, 918, 1279 |
| AjnI | CCWGG | 4 cut(s) | 82, 566, 958, 1172 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1100, 1132 |
| AluBI | AGCT | 6 cut(s) | 53, 295, 506, 737, 1220, 1299 |
| AluI | AGCT | 6 cut(s) | 53, 295, 506, 737, 1220, 1299 |
| Alw21I | GWGCWC | 2 cut(s) | 981, 1301 |
| Alw26I | GTCTC | 1 cut(s) | 645 |
| AlwI | GGATC | 2 cut(s) | 309, 489 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 878 |
| Ama87I | CYCGRG | 1 cut(s) | 775 |
| AoxI | GGCC | 4 cut(s) | 102, 214, 579, 654 |
| ApeKI | GCWGC | 4 cut(s) | 227, 350, 734, 769 |
| ApoI | RAATTY | 1 cut(s) | 198 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 409, 687 |
| Asp718I | GGTACC | 1 cut(s) | 448 |
| AspLEI | GCGC | 1 cut(s) | 128 |
| AspS9I | GGNCC | 3 cut(s) | 214, 1078, 1248 |
| AsuHPI | GGTGA | 1 cut(s) | 355 |
| AsuII | TTCGAA | 1 cut(s) | 681 |
| AvaI | CYCGRG | 1 cut(s) | 775 |
| AvaII | GGWCC | 2 cut(s) | 1078, 1248 |
| BalI | TGGCCA | 1 cut(s) | 656 |
| BanI | GGYRCC | 2 cut(s) | 448, 863 |
| BanII | GRGCYC | 2 cut(s) | 840, 1301 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 29 |
| BbsI | GAAGAC | 2 cut(s) | 213, 1006 |
| Bbv12I | GWGCWC | 2 cut(s) | 981, 1301 |
| BbvI | GCAGC | 4 cut(s) | 239, 337, 746, 781 |
| BccI | CCATC | 2 cut(s) | 607, 1282 |
| BceAI | ACGGC | 1 cut(s) | 566 |
| BciT130I | CCWGG | 4 cut(s) | 84, 568, 960, 1174 |
| BcoDI | GTCTC | 1 cut(s) | 645 |
| BfaI | CTAG | 2 cut(s) | 1364, 1391 |
| BfmI | CTRYAG | 2 cut(s) | 168, 615 |
| BfuAI | ACCTGC | 1 cut(s) | 899 |
| BisI | GCNGC | 4 cut(s) | 228, 351, 735, 770 |
| BlpI | GCTNAGC | 1 cut(s) | 1300 |
| BlsI | GCNGC | 4 cut(s) | 229, 352, 736, 771 |
| Bme1390I | CCNGG | 4 cut(s) | 84, 568, 960, 1174 |
| Bme18I | GGWCC | 2 cut(s) | 1078, 1248 |
| BmeT110I | CYCGRG | 1 cut(s) | 775 |
| BmgT120I | GGNCC | 3 cut(s) | 214, 1078, 1248 |
| BmiI | GGNNCC | 3 cut(s) | 148, 450, 865 |
| BmrFI | CCNGG | 4 cut(s) | 84, 568, 960, 1174 |
| BmsI | GCATC | 4 cut(s) | 435, 706, 895, 1415 |
| BpiI | GAAGAC | 2 cut(s) | 213, 1006 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 758, 790, 822, 854 |
| BpmI | CTGGAG | 1 cut(s) | 66 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1300 |
| Bpu14I | TTCGAA | 1 cut(s) | 681 |
| BpuEI | CTTGAG | 4 cut(s) | 321, 711, 991, 1060 |
| BsaBI | GATNNNNATC | 1 cut(s) | 178 |
| BsaHI | GRCGYC | 1 cut(s) | 72 |
| BsaJI | CCNNGG | 8 cut(s) | 99, 366, 477, 550, 576, 644, 1172, 1173 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1257, 1335 |
| Bse1I | ACTGG | 1 cut(s) | 543 |
| Bse3DI | GCAATG | 1 cut(s) | 1365 |
| Bse8I | GATNNNNATC | 1 cut(s) | 178 |
| BseBI | CCWGG | 4 cut(s) | 84, 568, 960, 1174 |
| BseDI | CCNNGG | 8 cut(s) | 99, 366, 477, 550, 576, 644, 1172, 1173 |
| BseGI | GGATG | 1 cut(s) | 179 |
| BseJI | GATNNNNATC | 1 cut(s) | 178 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1257, 1335 |
| BseMI | GCAATG | 1 cut(s) | 1365 |
| BseMII | CTCAG | 4 cut(s) | 113, 476, 1314, 1338 |
| BseNI | ACTGG | 1 cut(s) | 543 |
| BseRI | GAGGAG | 2 cut(s) | 85, 88 |
| BseXI | GCAGC | 4 cut(s) | 239, 337, 746, 781 |
| BseYI | CCCAGC | 1 cut(s) | 353 |
| Bsh1236I | CGCG | 1 cut(s) | 431 |
| BshFI | GGCC | 4 cut(s) | 104, 216, 581, 656 |
| BshNI | GGYRCC | 2 cut(s) | 448, 863 |
| BsiHKAI | GWGCWC | 2 cut(s) | 981, 1301 |
| BsiHKCI | CYCGRG | 1 cut(s) | 775 |
| BsiSI | CCGG | 1 cut(s) | 1370 |
| BslFI | GGGAC | 2 cut(s) | 829, 943 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1257, 1335 |
| BsmAI | GTCTC | 1 cut(s) | 645 |
| BsmFI | GGGAC | 2 cut(s) | 829, 943 |
| BsmI | GAATGC | 2 cut(s) | 718, 1064 |
| BsnI | GGCC | 4 cut(s) | 104, 216, 581, 656 |
| BsoBI | CYCGRG | 1 cut(s) | 775 |
| Bsp119I | TTCGAA | 1 cut(s) | 681 |
| Bsp1286I | GDGCHC | 3 cut(s) | 840, 981, 1301 |
| Bsp1407I | TGTACA | 1 cut(s) | 607 |
| Bsp143I | GATC | 2 cut(s) | 301, 481 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1300 |
| Bsp19I | CCATGG | 1 cut(s) | 366 |
| Bsp68I | TCGCGA | 1 cut(s) | 431 |
| BspACI | CCGC | 1 cut(s) | 1335 |
| BspANI | GGCC | 4 cut(s) | 104, 216, 581, 656 |
| BspCNI | CTCAG | 4 cut(s) | 114, 477, 1313, 1337 |
| BspFNI | CGCG | 1 cut(s) | 431 |
| BspHI | TCATGA | 1 cut(s) | 1284 |
| BspLI | GGNNCC | 3 cut(s) | 148, 450, 865 |
| BspMI | ACCTGC | 1 cut(s) | 899 |
| BspPI | GGATC | 2 cut(s) | 309, 489 |
| BspT104I | TTCGAA | 1 cut(s) | 681 |
| BspT107I | GGYRCC | 2 cut(s) | 448, 863 |
| BsrDI | GCAATG | 1 cut(s) | 1365 |
| BsrGI | TGTACA | 1 cut(s) | 607 |
| BsrI | ACTGG | 1 cut(s) | 543 |
| BssECI | CCNNGG | 8 cut(s) | 99, 366, 477, 550, 576, 644, 1172, 1173 |
| BssMI | GATC | 2 cut(s) | 301, 481 |
| BssNAI | GTATAC | 1 cut(s) | 457 |
| BssNI | GRCGYC | 1 cut(s) | 72 |
| BssT1I | CCWWGG | 5 cut(s) | 366, 477, 550, 576, 644 |
| Bst1107I | GTATAC | 1 cut(s) | 457 |
| Bst2UI | CCWGG | 4 cut(s) | 84, 568, 960, 1174 |
| Bst4CI | ACNGT | 2 cut(s) | 559, 1345 |
| Bst6I | CTCTTC | 1 cut(s) | 797 |
| BstACI | GRCGYC | 1 cut(s) | 72 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 383, 1412 |
| BstAUI | TGTACA | 1 cut(s) | 607 |
| BstBI | TTCGAA | 1 cut(s) | 681 |
| BstC8I | GCNNGC | 3 cut(s) | 654, 1306, 1404 |
| BstDEI | CTNAG | 5 cut(s) | 122, 485, 701, 1300, 1324 |
| BstDSI | CCRYGG | 1 cut(s) | 366 |
| BstF5I | GGATG | 1 cut(s) | 179 |
| BstFNI | CGCG | 1 cut(s) | 431 |
| BstHHI | GCGC | 1 cut(s) | 128 |
| BstKTI | GATC | 2 cut(s) | 304, 484 |
| BstMAI | GTCTC | 1 cut(s) | 645 |
| BstMBI | GATC | 2 cut(s) | 301, 481 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 134, 383, 1305, 1412 |
| BstNI | CCWGG | 4 cut(s) | 84, 568, 960, 1174 |
| BstSCI | CCNGG | 4 cut(s) | 82, 566, 958, 1172 |
| BstSFI | CTRYAG | 2 cut(s) | 168, 615 |
| BstUI | CGCG | 1 cut(s) | 431 |
| BstV1I | GCAGC | 4 cut(s) | 239, 337, 746, 781 |
| BstV2I | GAAGAC | 2 cut(s) | 213, 1006 |
| BstX2I | RGATCY | 2 cut(s) | 301, 481 |
| BstYI | RGATCY | 2 cut(s) | 301, 481 |
| BstZ17I | GTATAC | 1 cut(s) | 457 |
| BsuRI | GGCC | 4 cut(s) | 104, 216, 581, 656 |
| BtgI | CCRYGG | 1 cut(s) | 366 |
| BtgZI | GCGATG | 1 cut(s) | 412 |
| BtsCI | GGATG | 1 cut(s) | 179 |
| BtsI | GCAGTG | 1 cut(s) | 73 |
| BtsIMutI | CAGTG | 3 cut(s) | 73, 352, 536 |
| BtuMI | TCGCGA | 1 cut(s) | 431 |
| BveI | ACCTGC | 1 cut(s) | 899 |
| Cac8I | GCNNGC | 3 cut(s) | 654, 1306, 1404 |
| CaiI | CAGNNNCTG | 1 cut(s) | 878 |
| CciI | TCATGA | 1 cut(s) | 1284 |
| CfoI | GCGC | 1 cut(s) | 128 |
| Cfr13I | GGNCC | 3 cut(s) | 214, 1078, 1248 |
| Csp6I | GTAC | 3 cut(s) | 321, 449, 608 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 94, 129 |
| CviAII | CATG | 4 cut(s) | 367, 745, 841, 1285 |
| CviQI | GTAC | 3 cut(s) | 321, 449, 608 |
| DdeI | CTNAG | 5 cut(s) | 122, 485, 701, 1300, 1324 |
| DpnI | GATC | 2 cut(s) | 303, 483 |
| DpnII | GATC | 2 cut(s) | 301, 481 |
| DraIII | CACNNNGTG | 1 cut(s) | 383 |
| EaeI | YGGCCR | 3 cut(s) | 102, 579, 654 |
| Eam1104I | CTCTTC | 1 cut(s) | 797 |
| EarI | CTCTTC | 1 cut(s) | 797 |
| Ecl136II | GAGCTC | 1 cut(s) | 1299 |
| Eco130I | CCWWGG | 5 cut(s) | 366, 477, 550, 576, 644 |
| Eco24I | GRGCYC | 2 cut(s) | 840, 1301 |
| Eco47I | GGWCC | 2 cut(s) | 1078, 1248 |
| Eco53kI | GAGCTC | 1 cut(s) | 1299 |
| Eco57I | CTGAAG | 2 cut(s) | 486, 677 |
| Eco88I | CYCGRG | 1 cut(s) | 775 |
| EcoICRI | GAGCTC | 1 cut(s) | 1299 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1078 |
| EcoRII | CCWGG | 4 cut(s) | 82, 566, 958, 1172 |
| EcoT14I | CCWWGG | 5 cut(s) | 366, 477, 550, 576, 644 |
| EcoT38I | GRGCYC | 2 cut(s) | 840, 1301 |
| ErhI | CCWWGG | 5 cut(s) | 366, 477, 550, 576, 644 |
| FaeI | CATG | 4 cut(s) | 370, 748, 844, 1288 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 484, 516, 675, 707 |
| FaqI | GGGAC | 2 cut(s) | 829, 943 |
| FatI | CATG | 4 cut(s) | 366, 744, 840, 1284 |
| FauI | CCCGC | 1 cut(s) | 1328 |
| FblI | GTMKAC | 2 cut(s) | 456, 667 |
| Fnu4HI | GCNGC | 4 cut(s) | 228, 351, 735, 770 |
| FokI | GGATG | 1 cut(s) | 166 |
| FriOI | GRGCYC | 2 cut(s) | 840, 1301 |
| Fsp4HI | GCNGC | 4 cut(s) | 228, 351, 735, 770 |
| FspBI | CTAG | 2 cut(s) | 1364, 1391 |
| GlaI | GCGC | 1 cut(s) | 127 |
| GluI | GCNGC | 4 cut(s) | 228, 351, 735, 770 |
| GsaI | CCCAGC | 1 cut(s) | 357 |
| GsuI | CTGGAG | 1 cut(s) | 66 |
| HaeIII | GGCC | 4 cut(s) | 104, 216, 581, 656 |
| HapII | CCGG | 1 cut(s) | 1370 |
| HhaI | GCGC | 1 cut(s) | 128 |
| Hin1I | GRCGYC | 1 cut(s) | 72 |
| Hin1II | CATG | 4 cut(s) | 370, 748, 844, 1288 |
| Hin6I | GCGC | 1 cut(s) | 126 |
| HinP1I | GCGC | 1 cut(s) | 126 |
| HincII | GTYRAC | 1 cut(s) | 1341 |
| HindII | GTYRAC | 1 cut(s) | 1341 |
| HinfI | GANTC | 3 cut(s) | 191, 638, 955 |
| HpaI | GTTAAC | 1 cut(s) | 1341 |
| HpaII | CCGG | 1 cut(s) | 1370 |
| HphI | GGTGA | 1 cut(s) | 355 |
| Hpy166II | GTNNAC | 6 cut(s) | 363, 457, 498, 610, 668, 1341 |
| Hpy188I | TCNGA | 3 cut(s) | 123, 486, 1189 |
| Hpy188III | TCNNGA | 9 cut(s) | 195, 430, 635, 728, 777, 799, 1076, 1285, 1391 |
| Hpy8I | GTNNAC | 6 cut(s) | 363, 457, 498, 610, 668, 1341 |
| HpyAV | CCTTC | 4 cut(s) | 399, 486, 1087, 1285 |
| HpyCH4III | ACNGT | 2 cut(s) | 559, 1345 |
| HpyCH4IV | ACGT | 2 cut(s) | 72, 1206 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 134, 383, 1305, 1412 |
| HpyF3I | CTNAG | 5 cut(s) | 122, 485, 701, 1300, 1324 |
| HpySE526I | ACGT | 2 cut(s) | 72, 1206 |
| Hsp92I | GRCGYC | 1 cut(s) | 72 |
| Hsp92II | CATG | 4 cut(s) | 370, 748, 844, 1288 |
| HspAI | GCGC | 1 cut(s) | 126 |
| KpnI | GGTACC | 1 cut(s) | 452 |
| KspAI | GTTAAC | 1 cut(s) | 1341 |
| Kzo9I | GATC | 2 cut(s) | 301, 481 |
| LmnI | GCTCC | 2 cut(s) | 50, 1296 |
| Lsp1109I | GCAGC | 4 cut(s) | 239, 337, 746, 781 |
| LweI | GCATC | 4 cut(s) | 435, 706, 895, 1415 |
| MaeI | CTAG | 2 cut(s) | 1364, 1391 |
| MaeII | ACGT | 2 cut(s) | 72, 1206 |
| MaeIII | GTNAC | 3 cut(s) | 68, 1099, 1264 |
| MalI | GATC | 2 cut(s) | 303, 483 |
| MboI | GATC | 2 cut(s) | 301, 481 |
| MboII | GAAGA | 8 cut(s) | 17, 218, 683, 814, 1011, 1309, 1312, 1406 |
| MfeI | CAATTG | 3 cut(s) | 258, 939, 1410 |
| MflI | RGATCY | 2 cut(s) | 301, 481 |
| MhlI | GDGCHC | 3 cut(s) | 840, 981, 1301 |
| MlsI | TGGCCA | 1 cut(s) | 656 |
| MluNI | TGGCCA | 1 cut(s) | 656 |
| MlyI | GAGTC | 1 cut(s) | 647 |
| MmeI | TCCRAC | 2 cut(s) | 808, 976 |
| Mox20I | TGGCCA | 1 cut(s) | 656 |
| MroXI | GAANNNNTTC | 2 cut(s) | 409, 687 |
| MscI | TGGCCA | 1 cut(s) | 656 |
| MseI | TTAA | 3 cut(s) | 983, 1209, 1340 |
| MslI | CAYNNNNRTG | 1 cut(s) | 858 |
| Msp20I | TGGCCA | 1 cut(s) | 656 |
| MspA1I | CMGCKG | 2 cut(s) | 737, 1220 |
| MspI | CCGG | 1 cut(s) | 1370 |
| MspR9I | CCNGG | 4 cut(s) | 84, 568, 960, 1174 |
| MunI | CAATTG | 3 cut(s) | 258, 939, 1410 |
| Mva1269I | GAATGC | 2 cut(s) | 718, 1064 |
| MvaI | CCWGG | 4 cut(s) | 84, 568, 960, 1174 |
| MvnI | CGCG | 1 cut(s) | 431 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 134, 383, 1305, 1412 |
| NcoI | CCATGG | 1 cut(s) | 366 |
| NdeII | GATC | 2 cut(s) | 301, 481 |
| NlaIII | CATG | 4 cut(s) | 370, 748, 844, 1288 |
| NlaIV | GGNNCC | 3 cut(s) | 148, 450, 865 |
| NmeAIII | GCCGAG | 1 cut(s) | 80 |
| NmuCI | GTSAC | 2 cut(s) | 68, 1099 |
| NruI | TCGCGA | 1 cut(s) | 431 |
| NspV | TTCGAA | 1 cut(s) | 681 |
| PagI | TCATGA | 1 cut(s) | 1284 |
| PasI | CCCWGGG | 1 cut(s) | 1173 |
| PctI | GAATGC | 2 cut(s) | 718, 1064 |
| PdmI | GAANNNNTTC | 2 cut(s) | 409, 687 |
| PfeI | GAWTC | 2 cut(s) | 191, 955 |
| PfoI | TCCNGGA | 1 cut(s) | 82 |
| PkrI | GCNGC | 4 cut(s) | 229, 352, 736, 771 |
| PleI | GAGTC | 1 cut(s) | 646 |
| PpsI | GAGTC | 1 cut(s) | 646 |
| PpuMI | RGGWCCY | 1 cut(s) | 1078 |
| PsiI | TTATAA | 1 cut(s) | 1181 |
| Psp124BI | GAGCTC | 1 cut(s) | 1301 |
| Psp1406I | AACGTT | 1 cut(s) | 1206 |
| Psp5II | RGGWCCY | 1 cut(s) | 1078 |
| Psp6I | CCWGG | 4 cut(s) | 82, 566, 958, 1172 |
| PspFI | CCCAGC | 1 cut(s) | 353 |
| PspGI | CCWGG | 4 cut(s) | 82, 566, 958, 1172 |
| PspN4I | GGNNCC | 3 cut(s) | 148, 450, 865 |
| PspPI | GGNCC | 3 cut(s) | 214, 1078, 1248 |
| PspPPI | RGGWCCY | 1 cut(s) | 1078 |
| PstNI | CAGNNNCTG | 1 cut(s) | 878 |
| PsuI | RGATCY | 2 cut(s) | 301, 481 |
| PvuII | CAGCTG | 2 cut(s) | 737, 1220 |
| RruI | TCGCGA | 1 cut(s) | 431 |
| RsaI | GTAC | 3 cut(s) | 322, 450, 609 |
| RsaNI | GTAC | 3 cut(s) | 321, 449, 608 |
| RseI | CAYNNNNRTG | 1 cut(s) | 858 |
| SacI | GAGCTC | 1 cut(s) | 1301 |
| SaqAI | TTAA | 3 cut(s) | 983, 1209, 1340 |
| SatI | GCNGC | 4 cut(s) | 228, 351, 735, 770 |
| Sau3AI | GATC | 2 cut(s) | 301, 481 |
| Sau96I | GGNCC | 3 cut(s) | 214, 1078, 1248 |
| SchI | GAGTC | 1 cut(s) | 647 |
| ScrFI | CCNGG | 4 cut(s) | 84, 568, 960, 1174 |
| SduI | GDGCHC | 3 cut(s) | 840, 981, 1301 |
| SfaNI | GCATC | 4 cut(s) | 435, 706, 895, 1415 |
| SfcI | CTRYAG | 2 cut(s) | 168, 615 |
| SfuI | TTCGAA | 1 cut(s) | 681 |
| SinI | GGWCC | 2 cut(s) | 1078, 1248 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 858 |
| SmlI | CTYRAG | 4 cut(s) | 336, 726, 970, 1039 |
| SmoI | CTYRAG | 4 cut(s) | 336, 726, 970, 1039 |
| SsiI | CCGC | 1 cut(s) | 1335 |
| SspMI | CTAG | 2 cut(s) | 1364, 1391 |
| SstI | GAGCTC | 1 cut(s) | 1301 |
| StyD4I | CCNGG | 4 cut(s) | 82, 566, 958, 1172 |
| StyI | CCWWGG | 5 cut(s) | 366, 477, 550, 576, 644 |
| TaaI | ACNGT | 2 cut(s) | 559, 1345 |
| TaiI | ACGT | 2 cut(s) | 75, 1209 |
| TaqI | TCGA | 1 cut(s) | 681 |
| TatI | WGTACW | 1 cut(s) | 607 |
| TfiI | GAWTC | 2 cut(s) | 191, 955 |
| Tru1I | TTAA | 3 cut(s) | 983, 1209, 1340 |
| Tru9I | TTAA | 3 cut(s) | 983, 1209, 1340 |
| TscAI | CASTG | 3 cut(s) | 73, 352, 543 |
| TseFI | GTSAC | 2 cut(s) | 68, 1099 |
| TseI | GCWGC | 4 cut(s) | 227, 350, 734, 769 |
| Tsp45I | GTSAC | 2 cut(s) | 68, 1099 |
| TspDTI | ATGAA | 5 cut(s) | 168, 402, 619, 1108, 1273 |
| TspGWI | ACGGA | 1 cut(s) | 314 |
| TspRI | CASTG | 3 cut(s) | 73, 352, 543 |
| VpaK11BI | GGWCC | 2 cut(s) | 1078, 1248 |
| XapI | RAATTY | 1 cut(s) | 198 |
| XbaI | TCTAGA | 1 cut(s) | 1390 |
| XmiI | GTMKAC | 2 cut(s) | 456, 667 |
| XmnI | GAANNNNTTC | 2 cut(s) | 409, 687 |
| XspI | CTAG | 2 cut(s) | 1364, 1391 |
| ZraI | GACGTC | 1 cut(s) | 73 |