MD12G1226400.v1.1
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000401
Physical Location & Seq
Reverse (-)
30198582 .. 30202188
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1226400.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGAAGAAGGACTACTAAGAGACGTGTCCAGTAATGTAAATGGGGATGCAGTTGATGGGAGTGAAACAATGCCCGGTGGGTCCCCATCAGTCACCATTGTTGTTGTTCTAAGCACTTTGGTGGCCCTCTGTGGATCTCTCTCTGCTGGCTGTGCCACAGGATATTCATCAGCTGCTGAATCTGGGATCGTTGAAGACTTGGACCTTACTCTTGCTGCCTACTCAGTTTTCGGTTCAATACTGACAGTAGGAGGAATGATTGGCGGATTACTAAATGGAAAAATGGCGGATATGATCGGTCGTAAGGGTACAATGGGAGTCTCTGCAATATTCAGTTCTGCAGGGTGGCTCACTATTGCATTTGCCAAGAATGCTTGGTGGCTGGATACTGGACGACTGTCAGTGGGATTTGGCGTGGGGATAATTATGTACGTGGTACCTGTCTACATATCAGAAATAACACCTAAGGATCTCAGAGGAACATTTACTTCAGCTAATCAGTTGTTCATTAGCTGCGGTTTTTCGCTATTCTATTTCATTGGAAACGCCCTACCCTGGCGCACCATTGCAGTAATCGGTGCAATTCCAACTCTACTACAAATTATAGGTTTATTTTTCATCCCAGAGTCTCCAAGGTGGCTGGCCAAAATTGGTAAACAAAAAGACCTTGAAGCCACTCTGCAGCGTCTTAGAGGAACGGAGGCTTCTGTATCTCAAGAAGCAGCTGATATCATGGATTATACAGTAATGTTTCAGCAGCACTCAGAAAGTATGCTAGACTTGTTCCATAAGAGATATGCTGACTCGCTCATCATTGGTGTCGGGCTGATGTTTCTACAACAATTTTCGGGGGCTAACGCGACAGCTTTTTATGGAAGCTCTATATTTGTGGATGCCGATTTTTCAAGTAGTGTCGGGACTATTGCATTGGCTATTAGCGGGATACCTTCTGTTGCATTGTGTGTTTTCTTGACGGACAAATCTGGACGACGACCACTTCTAATGGTTTCTGCTGCCGGAATGTGCTTGAGTTTGTTTCTTGTGGGCTTGGCATTCTATTTTCAGGACCTCGACCTGTGGAAGAAAGGAACTCCAGTTCTCGTGCTCATCGGCATGCTGGGTTATGGTGCGACATACTTGATCGGAATGTCAGGATTACCGTGGGTTATTATGTCAGAGATTTTCCCGGTAAACATTAAGGGTTCGGCCGGAAGCCTGTTGAGTTTGGTGTACTGGTCTTGTTCTTGGATAACAACTTACACATTCAATTTTATGATGGAGTGGAGCTCGGCAGGAACGTTTTTCTTCTACGCGGCCATAGCCGGTTTAACTGTTTTGTTCATAGCAAAGCTAGTGCCGGAGACCAAGGGGAGAACCCTAGAAGAAATACAAGCATCAATCGCCCATTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

472

Amino Acids

50.36

Weight (kDa)

5.01

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 42 - 411 7.1e-27 Major Facilitator Superfamily
Sugar_tr PF00083 70 - 465 7.5e-87 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08920 AT1G08920 AT1G08920 AT3G05160 AT3G05160 AT3G05160 AT3G05165 AT3G05165 AT3G05165 AT3G05165 AT3G05165 AT3G05165 AT5G27350 AT5G27360 AT5G27360 AT5G27360 AT5G27360 AT5G27360
fragaria_vesca FvH4_6g05021 FvH4_6g05050 FvH4_6g05051 FvH4_6g05051 FvH4_6g05060 FvH4_6g05061 FvH4_6g05090 FvH4_6g05090 FvH4_6g05090 FvH4_6g05111 FvH4_6g05111 FvH4_6g05120
malus_domestica MD12G1225600.v1.1 MD12G1225900.v1.1 MD12G1226400.v1.1
pyrus_communis pycom12g21050 pycom12g21080
rosa_chinensis RchiOBHm_Chr3g0453541 RchiOBHm_Chr3g0453551 RchiOBHm_Chr3g0453561 RchiOBHm_Chr3g0453571 RchiOBHm_Chr3g0453591 RchiOBHm_Chr3g0453601 RchiOBHm_Chr3g0453621 RchiOBHm_Chr3g0453651
rosa_laevigata RLG00000025501 RLG00000025504 RLG00000025505
rosa_multiflora Rmu_co8451765.1_g000001 Rmu_sc0000510.1_g000021 Rmu_sc0000510.1_g000022 Rmu_sc0000510.1_g000023 Rmu_sc0000510.1_g000027 Rmu_sc0003556.1_g000001 Rmu_sc0003556.1_g000003
rosa_roxburghii Rroxscaffold_5G00359890 Rroxscaffold_5G00359900 Rroxscaffold_6G00430520 Rroxscaffold_6G00430530 Rroxscaffold_6G00430540 Rroxscaffold_6G00430550 Rroxscaffold_6G00430570
rosa_rugosa Rorug02G0649800 Rorug02G0649900 Rorug02G0650000 Rorug02G0650000 Rorug02G0650000 Rorug02G0650000 Rorug02G0650000 Rorug02G0650100 Rorug02G0650200 Rorug02G0650300 Rorug02G0650400 Rorug02G0650500 Rorug04G0138300
rosa_samantha Rh3AG052800 Rh3AG052900 Rh3AG053000 Rh3AG053100 Rh3AG053200 Rh3AG053400 Rh3BG054300 Rh3BG054400 Rh3BG054500 Rh3BG054600 Rh3BG054700 Rh3BG054900 Rh3CG053600 Rh3CG053700 Rh3CG053800 Rh3CG053900 Rh3CG054100 Rh3DG054400 Rh3DG054500 Rh3DG054700 Rh3DG054800 Rh3DG055000 Rh3DG055200 Rh4DG197900
rosa_wichuraiana Rw3G004070 Rw3G004080 Rw3G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 436
AccI GTMKAC 1 cut(s) 444
AccII CGCG 2 cut(s) 860, 1313
AciI CCGC 5 cut(s) 264, 287, 516, 939, 1313
AclI AACGTT 1 cut(s) 1298
AclWI GGATC 3 cut(s) 142, 194, 477
AcoI YGGCCR 3 cut(s) 642, 1206, 1314
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 4 cut(s) 310, 431, 438, 1232
AfiI CCNNNNNNNGG 1 cut(s) 555
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 5 cut(s) 194, 237, 671, 906, 1267
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 554
AleI CACNNNNGTG 1 cut(s) 119
AloI GAACNNNNNNTCC 2 cut(s) 1080, 1112
AluBI AGCT 8 cut(s) 173, 494, 513, 725, 866, 879, 1287, 1351
AluI AGCT 8 cut(s) 173, 494, 513, 725, 866, 879, 1287, 1351
Alw21I GWGCWC 2 cut(s) 1107, 1289
Alw26I GTCTC 4 cut(s) 15, 325, 633, 1355
AlwI GGATC 3 cut(s) 142, 194, 477
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 4 cut(s) 123, 642, 1206, 1314
ApeKI GCWGC 7 cut(s) 173, 215, 513, 682, 722, 757, 1013
Asp718I GGTACC 1 cut(s) 436
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 4 cut(s) 81, 124, 202, 1066
AsuC2I CCSGG 2 cut(s) 75, 1187
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 3 cut(s) 81, 202, 1066
AxyI CCTNAGG 1 cut(s) 465
BalI TGGCCA 1 cut(s) 644
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 1289
BarI GAAGNNNNNNTAC 1 cut(s) 29
BauI CACGAG 1 cut(s) 1100
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 1107, 1289
BbvI GCAGC 7 cut(s) 160, 202, 500, 694, 734, 769, 1000
BccI CCATC 3 cut(s) 50, 94, 1270
BciT130I CCWGG 1 cut(s) 556
BciVI GTATCC 2 cut(s) 379, 936
BcnI CCSGG 2 cut(s) 75, 1187
BcoDI GTCTC 4 cut(s) 15, 325, 633, 1355
BfaI CTAG 3 cut(s) 776, 1352, 1379
BfmI CTRYAG 2 cut(s) 339, 680
BfuI GTATCC 2 cut(s) 379, 936
BisI GCNGC 8 cut(s) 174, 216, 514, 683, 723, 758, 1014, 1314
BlsI GCNGC 8 cut(s) 175, 217, 515, 684, 724, 759, 1015, 1315
Bme1390I CCNGG 3 cut(s) 75, 556, 1187
Bme18I GGWCC 3 cut(s) 81, 202, 1066
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 81, 124, 202, 1066
BmiI GGNNCC 3 cut(s) 82, 83, 438
BmrFI CCNGG 3 cut(s) 75, 556, 1187
BmsI GCATC 3 cut(s) 37, 883, 1403
BoxI GACNNNNGTC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 201
BplI GAGNNNNNCTC 2 cut(s) 1271, 1303
BpmI CTGGAG 1 cut(s) 1077
BpuEI CTTGAG 2 cut(s) 699, 1048
BpuMI CCSGG 2 cut(s) 75, 1187
BsaAI YACGTR 1 cut(s) 433
BsaBI GATNNNNATC 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 1355
BsaJI CCNNGG 4 cut(s) 554, 632, 1160, 1365
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse118I RCCGGY 1 cut(s) 1322
Bse1I ACTGG 4 cut(s) 30, 394, 1094, 1238
Bse21I CCTNAGG 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 166
BseBI CCWGG 1 cut(s) 556
BseDI CCNNGG 4 cut(s) 554, 632, 1160, 1365
BseGI GGATG 3 cut(s) 52, 618, 898
BseJI GATNNNNATC 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 3 cut(s) 237, 487, 777
BseNI ACTGG 4 cut(s) 30, 394, 1094, 1238
BseX3I CGGCCG 1 cut(s) 1206
BseXI GCAGC 7 cut(s) 160, 202, 500, 694, 734, 769, 1000
BseYI CCCAGC 1 cut(s) 1117
Bsh1236I CGCG 2 cut(s) 860, 1313
Bsh1285I CGRYCG 2 cut(s) 301, 1209
BshFI GGCC 4 cut(s) 125, 644, 1208, 1316
BshNI GGYRCC 1 cut(s) 436
BsiEI CGRYCG 2 cut(s) 301, 1209
BsiHKAI GWGCWC 2 cut(s) 1107, 1289
BsiSI CCGG 6 cut(s) 75, 1017, 1187, 1209, 1323, 1358
BslFI GGGAC 2 cut(s) 67, 931
BslI CCNNNNNNNGG 1 cut(s) 555
BsmAI GTCTC 4 cut(s) 15, 325, 633, 1355
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 67, 931
BsmI GAATGC 2 cut(s) 376, 1052
BsnI GGCC 4 cut(s) 125, 644, 1208, 1316
Bso31I GGTCTC 1 cut(s) 1355
Bsp1286I GDGCHC 2 cut(s) 1107, 1289
Bsp143I GATC 5 cut(s) 134, 186, 294, 469, 1140
BspACI CCGC 5 cut(s) 264, 287, 516, 939, 1313
BspANI GGCC 4 cut(s) 125, 644, 1208, 1316
BspCNI CTCAG 3 cut(s) 236, 486, 776
BspFNI CGCG 2 cut(s) 860, 1313
BspLI GGNNCC 3 cut(s) 82, 83, 438
BspMAI CTGCAG 2 cut(s) 343, 684
BspPI GGATC 3 cut(s) 142, 194, 477
BspT107I GGYRCC 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 1355
BsrDI GCAATG 1 cut(s) 564
BsrFI RCCGGY 1 cut(s) 1322
BsrI ACTGG 4 cut(s) 30, 394, 1094, 1238
BssAI RCCGGY 1 cut(s) 1322
BssECI CCNNGG 4 cut(s) 554, 632, 1160, 1365
BssMI GATC 5 cut(s) 134, 186, 294, 469, 1140
BssSI CACGAG 1 cut(s) 1100
BssT1I CCWWGG 2 cut(s) 632, 1365
Bst2BI CACGAG 1 cut(s) 1100
Bst2UI CCWGG 1 cut(s) 556
Bst4CI ACNGT 5 cut(s) 247, 399, 745, 1161, 1333
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 148, 642, 1115
BstDEI CTNAG 7 cut(s) 17, 110, 223, 465, 473, 689, 763
BstDSI CCRYGG 1 cut(s) 1160
BstF5I GGATG 3 cut(s) 52, 618, 898
BstFNI CGCG 2 cut(s) 860, 1313
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 5 cut(s) 137, 189, 297, 472, 1143
BstMAI GTCTC 4 cut(s) 15, 325, 633, 1355
BstMBI GATC 5 cut(s) 134, 186, 294, 469, 1140
BstMCI CGRYCG 2 cut(s) 301, 1209
BstMWI GCNNNNNNNGC 4 cut(s) 152, 371, 1319, 1400
BstNI CCWGG 1 cut(s) 556
BstNSI RCATGY 1 cut(s) 1117
BstPAI GACNNNNGTC 1 cut(s) 397
BstSCI CCNGG 3 cut(s) 73, 554, 1185
BstSFI CTRYAG 2 cut(s) 339, 680
BstUI CGCG 2 cut(s) 860, 1313
BstV1I GCAGC 7 cut(s) 160, 202, 500, 694, 734, 769, 1000
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 2 cut(s) 134, 469
BstYI RGATCY 2 cut(s) 134, 469
BstZI CGGCCG 1 cut(s) 1206
Bsu36I CCTNAGG 1 cut(s) 465
BsuI GTATCC 2 cut(s) 379, 936
BsuRI GGCC 4 cut(s) 125, 644, 1208, 1316
BtgI CCRYGG 1 cut(s) 1160
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 3 cut(s) 52, 618, 898
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 3 cut(s) 148, 642, 1115
CaiI CAGNNNCTG 1 cut(s) 176
CfoI GCGC 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 1322
Cfr13I GGNCC 4 cut(s) 81, 124, 202, 1066
CseI GACGC 1 cut(s) 674
Csp6I GTAC 4 cut(s) 309, 430, 437, 1231
CviAII CATG 2 cut(s) 733, 1114
CviQI GTAC 4 cut(s) 309, 430, 437, 1231
DdeI CTNAG 7 cut(s) 17, 110, 223, 465, 473, 689, 763
DpnI GATC 5 cut(s) 136, 188, 296, 471, 1142
DpnII GATC 5 cut(s) 134, 186, 294, 469, 1140
EaeI YGGCCR 3 cut(s) 642, 1206, 1314
EagI CGGCCG 1 cut(s) 1206
EciI GGCGGA 2 cut(s) 279, 302
Ecl136II GAGCTC 1 cut(s) 1287
EclXI CGGCCG 1 cut(s) 1206
Eco130I CCWWGG 2 cut(s) 632, 1365
Eco24I GRGCYC 1 cut(s) 1289
Eco31I GGTCTC 1 cut(s) 1355
Eco32I GATATC 1 cut(s) 730
Eco47I GGWCC 3 cut(s) 81, 202, 1066
Eco52I CGGCCG 1 cut(s) 1206
Eco53kI GAGCTC 1 cut(s) 1287
Eco57I CTGAAG 1 cut(s) 474
Eco81I CCTNAGG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 1287
EcoO109I RGGNCCY 2 cut(s) 81, 1066
EcoRII CCWGG 1 cut(s) 554
EcoRV GATATC 1 cut(s) 730
EcoT14I CCWWGG 2 cut(s) 632, 1365
EcoT38I GRGCYC 1 cut(s) 1289
ErhI CCWWGG 2 cut(s) 632, 1365
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 2 cut(s) 736, 1117
FaqI GGGAC 2 cut(s) 67, 931
FatI CATG 2 cut(s) 732, 1113
FauI CCCGC 1 cut(s) 932
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 8 cut(s) 174, 216, 514, 683, 723, 758, 1014, 1314
FokI GGATG 3 cut(s) 59, 605, 905
FriOI GRGCYC 1 cut(s) 1289
Fsp4HI GCNGC 8 cut(s) 174, 216, 514, 683, 723, 758, 1014, 1314
FspBI CTAG 3 cut(s) 776, 1352, 1379
GlaI GCGC 1 cut(s) 560
GluI GCNGC 8 cut(s) 174, 216, 514, 683, 723, 758, 1014, 1314
GsaI CCCAGC 1 cut(s) 1121
GsuI CTGGAG 1 cut(s) 1077
HaeIII GGCC 4 cut(s) 125, 644, 1208, 1316
HapII CCGG 6 cut(s) 75, 1017, 1187, 1209, 1323, 1358
HgaI GACGC 1 cut(s) 674
HhaI GCGC 1 cut(s) 561
Hin1II CATG 2 cut(s) 736, 1117
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HinfI GANTC 4 cut(s) 179, 318, 626, 803
HpaII CCGG 6 cut(s) 75, 1017, 1187, 1209, 1323, 1358
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 4 cut(s) 445, 656, 1192, 1231
Hpy188I TCNGA 5 cut(s) 454, 476, 766, 1145, 1177
Hpy188III TCNNGA 6 cut(s) 716, 916, 970, 984, 1064, 1152
Hpy8I GTNNAC 4 cut(s) 445, 656, 1192, 1231
Hpy99I CGWCG 1 cut(s) 993
HpyAV CCTTC 1 cut(s) 957
HpyCH4III ACNGT 5 cut(s) 247, 399, 745, 1161, 1333
HpyCH4IV ACGT 3 cut(s) 24, 432, 1298
HpyCH4V TGCA 9 cut(s) 50, 326, 341, 359, 569, 581, 682, 926, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 371, 1319, 1400
HpyF3I CTNAG 7 cut(s) 17, 110, 223, 465, 473, 689, 763
HpySE526I ACGT 3 cut(s) 24, 432, 1298
Hsp92II CATG 2 cut(s) 736, 1117
HspAI GCGC 1 cut(s) 559
KflI GGGWCCC 1 cut(s) 81
KpnI GGTACC 1 cut(s) 440
Kzo9I GATC 5 cut(s) 134, 186, 294, 469, 1140
LmnI GCTCC 1 cut(s) 1284
Lsp1109I GCAGC 7 cut(s) 160, 202, 500, 694, 734, 769, 1000
LweI GCATC 3 cut(s) 37, 883, 1403
MaeI CTAG 3 cut(s) 776, 1352, 1379
MaeII ACGT 3 cut(s) 24, 432, 1298
MaeIII GTNAC 1 cut(s) 91
MalI GATC 5 cut(s) 136, 188, 296, 471, 1142
MboI GATC 5 cut(s) 134, 186, 294, 469, 1140
MboII GAAGA 5 cut(s) 17, 206, 1093, 1297, 1394
MflI RGATCY 2 cut(s) 134, 469
MhlI GDGCHC 2 cut(s) 1107, 1289
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 6 cut(s) 423, 582, 600, 648, 842, 1267
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 3 cut(s) 327, 635, 797
MmeI TCCRAC 1 cut(s) 611
MnlI CCTC 6 cut(s) 137, 245, 470, 686, 694, 1079
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MseI TTAA 3 cut(s) 1197, 1328, 1414
MslI CAYNNNNRTG 2 cut(s) 119, 1112
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 173, 725
MspI CCGG 6 cut(s) 75, 1017, 1187, 1209, 1323, 1358
MspR9I CCNGG 3 cut(s) 75, 556, 1187
Mva1269I GAATGC 2 cut(s) 376, 1052
MvaI CCWGG 1 cut(s) 556
MvnI CGCG 2 cut(s) 860, 1313
MwoI GCNNNNNNNGC 4 cut(s) 152, 371, 1319, 1400
NciI CCSGG 2 cut(s) 75, 1187
NdeII GATC 5 cut(s) 134, 186, 294, 469, 1140
NlaIII CATG 2 cut(s) 736, 1117
NlaIV GGNNCC 3 cut(s) 82, 83, 438
NmeAIII GCCGAG 1 cut(s) 1268
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 1 cut(s) 1117
OliI CACNNNNGTG 1 cut(s) 119
PaeI GCATGC 1 cut(s) 1117
PcsI WCGNNNNNNNCGW 1 cut(s) 1295
PctI GAATGC 2 cut(s) 376, 1052
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 8 cut(s) 175, 217, 515, 684, 724, 759, 1015, 1315
PleI GAGTC 3 cut(s) 326, 634, 797
PpsI GAGTC 3 cut(s) 326, 634, 797
Ppu21I YACGTR 1 cut(s) 433
PpuMI RGGWCCY 2 cut(s) 81, 1066
PshAI GACNNNNGTC 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 1289
Psp1406I AACGTT 1 cut(s) 1298
Psp5II RGGWCCY 2 cut(s) 81, 1066
Psp6I CCWGG 1 cut(s) 554
PspFI CCCAGC 1 cut(s) 1117
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 3 cut(s) 82, 83, 438
PspPI GGNCC 4 cut(s) 81, 124, 202, 1066
PspPPI RGGWCCY 2 cut(s) 81, 1066
PstI CTGCAG 2 cut(s) 343, 684
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 2 cut(s) 134, 469
PvuII CAGCTG 2 cut(s) 173, 725
RsaI GTAC 4 cut(s) 310, 431, 438, 1232
RsaNI GTAC 4 cut(s) 309, 430, 437, 1231
RseI CAYNNNNRTG 2 cut(s) 119, 1112
SacI GAGCTC 1 cut(s) 1289
SaqAI TTAA 3 cut(s) 1197, 1328, 1414
SatI GCNGC 8 cut(s) 174, 216, 514, 683, 723, 758, 1014, 1314
Sau3AI GATC 5 cut(s) 134, 186, 294, 469, 1140
Sau96I GGNCC 4 cut(s) 81, 124, 202, 1066
SchI GAGTC 3 cut(s) 327, 635, 797
ScrFI CCNGG 3 cut(s) 75, 556, 1187
SduI GDGCHC 2 cut(s) 1107, 1289
SfaNI GCATC 3 cut(s) 37, 883, 1403
SfcI CTRYAG 2 cut(s) 339, 680
SinI GGWCC 3 cut(s) 81, 202, 1066
SmiMI CAYNNNNRTG 2 cut(s) 119, 1112
SmlI CTYRAG 2 cut(s) 714, 1027
SmoI CTYRAG 2 cut(s) 714, 1027
SphI GCATGC 1 cut(s) 1117
Sse9I AATT 6 cut(s) 423, 582, 600, 648, 842, 1267
SsiI CCGC 5 cut(s) 264, 287, 516, 939, 1313
SspI AATATT 1 cut(s) 330
SspMI CTAG 3 cut(s) 776, 1352, 1379
SstI GAGCTC 1 cut(s) 1289
StyD4I CCNGG 3 cut(s) 73, 554, 1185
StyI CCWWGG 2 cut(s) 632, 1365
TaaI ACNGT 5 cut(s) 247, 399, 745, 1161, 1333
TaiI ACGT 3 cut(s) 27, 435, 1301
TaqI TCGA 1 cut(s) 1071
TaqII GACCGA 1 cut(s) 287
TasI AATT 6 cut(s) 423, 582, 600, 648, 842, 1267
TatI WGTACW 1 cut(s) 1230
TauI GCSGC 1 cut(s) 1316
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 3 cut(s) 1197, 1328, 1414
Tru9I TTAA 3 cut(s) 1197, 1328, 1414
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 7 cut(s) 173, 215, 513, 682, 722, 757, 1013
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 5 cut(s) 156, 496, 526, 607, 1330
TspGWI ACGGA 2 cut(s) 713, 989
TspRI CASTG 1 cut(s) 408
VpaK11BI GGWCC 3 cut(s) 81, 202, 1066
XceI RCATGY 1 cut(s) 1117
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 3 cut(s) 776, 1352, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.