Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 632 |
| Acc65I | GGTACC | 1 cut(s) | 181 |
| AccB1I | GGYRCC | 3 cut(s) | 181, 323, 596 |
| AccI | GTMKAC | 2 cut(s) | 189, 849 |
| AciI | CCGC | 5 cut(s) | 261, 304, 314, 419, 762 |
| AclWI | GGATC | 1 cut(s) | 222 |
| AcoI | YGGCCR | 1 cut(s) | 387 |
| AcsI | RAATTY | 1 cut(s) | 410 |
| AcuI | CTGAAG | 2 cut(s) | 219, 410 |
| AfaI | GTAC | 5 cut(s) | 55, 183, 342, 655, 778 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 593, 787 |
| AgsI | TTSAA | 2 cut(s) | 416, 651 |
| AjnI | CCWGG | 3 cut(s) | 299, 691, 905 |
| AluBI | AGCT | 3 cut(s) | 239, 470, 832 |
| AluI | AGCT | 3 cut(s) | 239, 470, 832 |
| Alw21I | GWGCWC | 2 cut(s) | 714, 834 |
| Alw26I | GTCTC | 2 cut(s) | 378, 681 |
| AlwI | GGATC | 1 cut(s) | 222 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 433 |
| AoxI | GGCC | 2 cut(s) | 352, 387 |
| ApeKI | GCWGC | 2 cut(s) | 467, 502 |
| ApoI | RAATTY | 1 cut(s) | 410 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 495, 516 |
| Asp718I | GGTACC | 1 cut(s) | 181 |
| AspS9I | GGNCC | 1 cut(s) | 306 |
| AsuHPI | GGTGA | 2 cut(s) | 88, 937 |
| AvaII | GGWCC | 1 cut(s) | 306 |
| BalI | TGGCCA | 1 cut(s) | 389 |
| BanI | GGYRCC | 3 cut(s) | 181, 323, 596 |
| BanII | GRGCYC | 2 cut(s) | 573, 834 |
| BbsI | GAAGAC | 1 cut(s) | 739 |
| Bbv12I | GWGCWC | 2 cut(s) | 714, 834 |
| BbvI | GCAGC | 2 cut(s) | 479, 514 |
| BccI | CCATC | 2 cut(s) | 84, 340 |
| BciT130I | CCWGG | 3 cut(s) | 301, 693, 907 |
| BcoDI | GTCTC | 2 cut(s) | 378, 681 |
| BfaI | CTAG | 2 cut(s) | 272, 521 |
| BfuAI | ACCTGC | 1 cut(s) | 632 |
| BisI | GCNGC | 4 cut(s) | 262, 420, 468, 503 |
| BlpI | GCTNAGC | 1 cut(s) | 265 |
| BlsI | GCNGC | 4 cut(s) | 263, 421, 469, 504 |
| Bme1390I | CCNGG | 3 cut(s) | 301, 693, 907 |
| Bme18I | GGWCC | 1 cut(s) | 306 |
| BmgT120I | GGNCC | 1 cut(s) | 306 |
| BmiI | GGNNCC | 3 cut(s) | 183, 325, 598 |
| BmrFI | CCNGG | 3 cut(s) | 301, 693, 907 |
| BmsI | GCATC | 1 cut(s) | 628 |
| BoxI | GACNNNNGTC | 1 cut(s) | 142 |
| BpiI | GAAGAC | 1 cut(s) | 739 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 555, 587 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 265 |
| BpuEI | CTTGAG | 2 cut(s) | 444, 793 |
| BsaAI | YACGTR | 1 cut(s) | 178 |
| BsaJI | CCNNGG | 6 cut(s) | 210, 283, 309, 377, 905, 906 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 593, 787 |
| BseBI | CCWGG | 3 cut(s) | 301, 693, 907 |
| BseDI | CCNNGG | 6 cut(s) | 210, 283, 309, 377, 905, 906 |
| BseGI | GGATG | 1 cut(s) | 95 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 593, 787 |
| BseMII | CTCAG | 3 cut(s) | 257, 425, 933 |
| BseXI | GCAGC | 2 cut(s) | 479, 514 |
| BsgI | GTGCAG | 1 cut(s) | 30 |
| BshFI | GGCC | 2 cut(s) | 354, 389 |
| BshNI | GGYRCC | 3 cut(s) | 181, 323, 596 |
| BsiHKAI | GWGCWC | 2 cut(s) | 714, 834 |
| BslFI | GGGAC | 1 cut(s) | 676 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 593, 787 |
| BsmAI | GTCTC | 2 cut(s) | 378, 681 |
| BsmFI | GGGAC | 1 cut(s) | 676 |
| BsmI | GAATGC | 2 cut(s) | 121, 797 |
| BsnI | GGCC | 2 cut(s) | 354, 389 |
| Bsp1286I | GDGCHC | 3 cut(s) | 573, 714, 834 |
| Bsp1407I | TGTACA | 1 cut(s) | 340 |
| Bsp143I | GATC | 2 cut(s) | 34, 214 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 265 |
| BspACI | CCGC | 5 cut(s) | 261, 304, 314, 419, 762 |
| BspANI | GGCC | 2 cut(s) | 354, 389 |
| BspCNI | CTCAG | 3 cut(s) | 256, 426, 934 |
| BspLI | GGNNCC | 3 cut(s) | 183, 325, 598 |
| BspMI | ACCTGC | 1 cut(s) | 632 |
| BspPI | GGATC | 1 cut(s) | 222 |
| BspQI | GCTCTTC | 1 cut(s) | 429 |
| BspT107I | GGYRCC | 3 cut(s) | 181, 323, 596 |
| BsrGI | TGTACA | 1 cut(s) | 340 |
| BssECI | CCNNGG | 6 cut(s) | 210, 283, 309, 377, 905, 906 |
| BssMI | GATC | 2 cut(s) | 34, 214 |
| BssNAI | GTATAC | 1 cut(s) | 190 |
| BssT1I | CCWWGG | 4 cut(s) | 210, 283, 309, 377 |
| Bst1107I | GTATAC | 1 cut(s) | 190 |
| Bst2UI | CCWGG | 3 cut(s) | 301, 693, 907 |
| Bst4CI | ACNGT | 1 cut(s) | 141 |
| Bst6I | CTCTTC | 1 cut(s) | 429 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 116 |
| BstAUI | TGTACA | 1 cut(s) | 340 |
| BstBAI | YACGTR | 1 cut(s) | 178 |
| BstC8I | GCNNGC | 3 cut(s) | 387, 447, 760 |
| BstDEI | CTNAG | 5 cut(s) | 218, 243, 265, 434, 942 |
| BstF5I | GGATG | 1 cut(s) | 95 |
| BstKTI | GATC | 2 cut(s) | 37, 217 |
| BstMAI | GTCTC | 2 cut(s) | 378, 681 |
| BstMBI | GATC | 2 cut(s) | 34, 214 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 116, 768 |
| BstNI | CCWGG | 3 cut(s) | 301, 693, 907 |
| BstNSI | RCATGY | 1 cut(s) | 449 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 142 |
| BstSCI | CCNGG | 3 cut(s) | 299, 691, 905 |
| BstV1I | GCAGC | 2 cut(s) | 479, 514 |
| BstV2I | GAAGAC | 1 cut(s) | 739 |
| BstX2I | RGATCY | 1 cut(s) | 214 |
| BstYI | RGATCY | 1 cut(s) | 214 |
| BstZ17I | GTATAC | 1 cut(s) | 190 |
| BsuRI | GGCC | 2 cut(s) | 354, 389 |
| BtsCI | GGATG | 1 cut(s) | 95 |
| BtsI | GCAGTG | 1 cut(s) | 578 |
| BtsIMutI | CAGTG | 3 cut(s) | 85, 153, 578 |
| BveI | ACCTGC | 1 cut(s) | 632 |
| Cac8I | GCNNGC | 3 cut(s) | 387, 447, 760 |
| CaiI | CAGNNNCTG | 1 cut(s) | 433 |
| Cfr13I | GGNCC | 1 cut(s) | 306 |
| Csp6I | GTAC | 5 cut(s) | 54, 182, 341, 654, 777 |
| CviAII | CATG | 2 cut(s) | 446, 892 |
| CviQI | GTAC | 5 cut(s) | 54, 182, 341, 654, 777 |
| DdeI | CTNAG | 5 cut(s) | 218, 243, 265, 434, 942 |
| DpnI | GATC | 2 cut(s) | 36, 216 |
| DpnII | GATC | 2 cut(s) | 34, 214 |
| EaeI | YGGCCR | 1 cut(s) | 387 |
| Eam1104I | CTCTTC | 1 cut(s) | 429 |
| EarI | CTCTTC | 1 cut(s) | 429 |
| EciI | GGCGGA | 2 cut(s) | 319, 777 |
| Ecl136II | GAGCTC | 1 cut(s) | 832 |
| Eco130I | CCWWGG | 4 cut(s) | 210, 283, 309, 377 |
| Eco147I | AGGCCT | 1 cut(s) | 354 |
| Eco24I | GRGCYC | 2 cut(s) | 573, 834 |
| Eco47I | GGWCC | 1 cut(s) | 306 |
| Eco53kI | GAGCTC | 1 cut(s) | 832 |
| Eco57I | CTGAAG | 2 cut(s) | 219, 410 |
| EcoICRI | GAGCTC | 1 cut(s) | 832 |
| EcoRII | CCWGG | 3 cut(s) | 299, 691, 905 |
| EcoT14I | CCWWGG | 4 cut(s) | 210, 283, 309, 377 |
| EcoT22I | ATGCAT | 1 cut(s) | 483 |
| EcoT38I | GRGCYC | 2 cut(s) | 573, 834 |
| ErhI | CCWWGG | 4 cut(s) | 210, 283, 309, 377 |
| FaeI | CATG | 2 cut(s) | 449, 895 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 217, 249, 408, 440 |
| FaqI | GGGAC | 1 cut(s) | 676 |
| FatI | CATG | 2 cut(s) | 445, 891 |
| FblI | GTMKAC | 2 cut(s) | 189, 849 |
| Fnu4HI | GCNGC | 4 cut(s) | 262, 420, 468, 503 |
| FokI | GGATG | 1 cut(s) | 102 |
| FriOI | GRGCYC | 2 cut(s) | 573, 834 |
| Fsp4HI | GCNGC | 4 cut(s) | 262, 420, 468, 503 |
| FspBI | CTAG | 2 cut(s) | 272, 521 |
| GluI | GCNGC | 4 cut(s) | 262, 420, 468, 503 |
| HaeIII | GGCC | 2 cut(s) | 354, 389 |
| Hin1II | CATG | 2 cut(s) | 449, 895 |
| HinfI | GANTC | 2 cut(s) | 371, 688 |
| HphI | GGTGA | 2 cut(s) | 88, 937 |
| Hpy166II | GTNNAC | 3 cut(s) | 190, 343, 850 |
| Hpy188I | TCNGA | 4 cut(s) | 70, 511, 731, 922 |
| Hpy188III | TCNNGA | 5 cut(s) | 368, 461, 521, 809, 823 |
| Hpy8I | GTNNAC | 3 cut(s) | 190, 343, 850 |
| HpyAV | CCTTC | 2 cut(s) | 791, 820 |
| HpyCH4III | ACNGT | 1 cut(s) | 141 |
| HpyCH4IV | ACGT | 1 cut(s) | 177 |
| HpyCH4V | TGCA | 8 cut(s) | 11, 86, 110, 481, 583, 605, 620, 641 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 116, 768 |
| HpyF3I | CTNAG | 5 cut(s) | 218, 243, 265, 434, 942 |
| HpySE526I | ACGT | 1 cut(s) | 177 |
| Hsp92II | CATG | 2 cut(s) | 449, 895 |
| KpnI | GGTACC | 1 cut(s) | 185 |
| Kzo9I | GATC | 2 cut(s) | 34, 214 |
| LguI | GCTCTTC | 1 cut(s) | 429 |
| LmnI | GCTCC | 1 cut(s) | 829 |
| Lsp1109I | GCAGC | 2 cut(s) | 479, 514 |
| LweI | GCATC | 1 cut(s) | 628 |
| MaeI | CTAG | 2 cut(s) | 272, 521 |
| MaeII | ACGT | 1 cut(s) | 177 |
| MaeIII | GTNAC | 2 cut(s) | 576, 929 |
| MalI | GATC | 2 cut(s) | 36, 216 |
| MboI | GATC | 2 cut(s) | 34, 214 |
| MboII | GAAGA | 5 cut(s) | 37, 237, 416, 744, 793 |
| MflI | RGATCY | 1 cut(s) | 214 |
| MhlI | GDGCHC | 3 cut(s) | 573, 714, 834 |
| MlsI | TGGCCA | 1 cut(s) | 389 |
| MluCI | AATT | 3 cut(s) | 168, 393, 410 |
| MluNI | TGGCCA | 1 cut(s) | 389 |
| MlyI | GAGTC | 1 cut(s) | 380 |
| MmeI | TCCRAC | 2 cut(s) | 541, 709 |
| MnlI | CCTC | 4 cut(s) | 44, 215, 431, 916 |
| Mox20I | TGGCCA | 1 cut(s) | 389 |
| Mph1103I | ATGCAT | 1 cut(s) | 483 |
| MroXI | GAANNNNTTC | 2 cut(s) | 495, 516 |
| MscI | TGGCCA | 1 cut(s) | 389 |
| MseI | TTAA | 1 cut(s) | 716 |
| Msp20I | TGGCCA | 1 cut(s) | 389 |
| MspA1I | CMGCKG | 1 cut(s) | 470 |
| MspR9I | CCNGG | 3 cut(s) | 301, 693, 907 |
| Mva1269I | GAATGC | 2 cut(s) | 121, 797 |
| MvaI | CCWGG | 3 cut(s) | 301, 693, 907 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 116, 768 |
| NdeII | GATC | 2 cut(s) | 34, 214 |
| NlaIII | CATG | 2 cut(s) | 449, 895 |
| NlaIV | GGNNCC | 3 cut(s) | 183, 325, 598 |
| NmuCI | GTSAC | 2 cut(s) | 576, 929 |
| NsiI | ATGCAT | 1 cut(s) | 483 |
| NspI | RCATGY | 1 cut(s) | 449 |
| PaeI | GCATGC | 1 cut(s) | 449 |
| PasI | CCCWGGG | 1 cut(s) | 906 |
| PceI | AGGCCT | 1 cut(s) | 354 |
| PciSI | GCTCTTC | 1 cut(s) | 429 |
| PctI | GAATGC | 2 cut(s) | 121, 797 |
| PdmI | GAANNNNTTC | 2 cut(s) | 495, 516 |
| PfeI | GAWTC | 1 cut(s) | 688 |
| PflFI | GACNNNGTC | 1 cut(s) | 725 |
| PkrI | GCNGC | 4 cut(s) | 263, 421, 469, 504 |
| PleI | GAGTC | 1 cut(s) | 379 |
| PpsI | GAGTC | 1 cut(s) | 379 |
| Ppu21I | YACGTR | 1 cut(s) | 178 |
| PshAI | GACNNNNGTC | 1 cut(s) | 142 |
| Psp124BI | GAGCTC | 1 cut(s) | 834 |
| Psp6I | CCWGG | 3 cut(s) | 299, 691, 905 |
| PspGI | CCWGG | 3 cut(s) | 299, 691, 905 |
| PspN4I | GGNNCC | 3 cut(s) | 183, 325, 598 |
| PspPI | GGNCC | 1 cut(s) | 306 |
| PstNI | CAGNNNCTG | 1 cut(s) | 433 |
| PsuI | RGATCY | 1 cut(s) | 214 |
| PsyI | GACNNNGTC | 1 cut(s) | 725 |
| PvuII | CAGCTG | 1 cut(s) | 470 |
| RsaI | GTAC | 5 cut(s) | 55, 183, 342, 655, 778 |
| RsaNI | GTAC | 5 cut(s) | 54, 182, 341, 654, 777 |
| SacI | GAGCTC | 1 cut(s) | 834 |
| SapI | GCTCTTC | 1 cut(s) | 429 |
| SaqAI | TTAA | 1 cut(s) | 716 |
| SatI | GCNGC | 4 cut(s) | 262, 420, 468, 503 |
| Sau3AI | GATC | 2 cut(s) | 34, 214 |
| Sau96I | GGNCC | 1 cut(s) | 306 |
| SchI | GAGTC | 1 cut(s) | 380 |
| ScrFI | CCNGG | 3 cut(s) | 301, 693, 907 |
| SduI | GDGCHC | 3 cut(s) | 573, 714, 834 |
| SfaNI | GCATC | 1 cut(s) | 628 |
| SinI | GGWCC | 1 cut(s) | 306 |
| SmlI | CTYRAG | 2 cut(s) | 459, 772 |
| SmoI | CTYRAG | 2 cut(s) | 459, 772 |
| SphI | GCATGC | 1 cut(s) | 449 |
| Sse9I | AATT | 3 cut(s) | 168, 393, 410 |
| SseBI | AGGCCT | 1 cut(s) | 354 |
| SsiI | CCGC | 5 cut(s) | 261, 304, 314, 419, 762 |
| SspMI | CTAG | 2 cut(s) | 272, 521 |
| SstI | GAGCTC | 1 cut(s) | 834 |
| StuI | AGGCCT | 1 cut(s) | 354 |
| StyD4I | CCNGG | 3 cut(s) | 299, 691, 905 |
| StyI | CCWWGG | 4 cut(s) | 210, 283, 309, 377 |
| TaaI | ACNGT | 1 cut(s) | 141 |
| TaiI | ACGT | 1 cut(s) | 180 |
| TasI | AATT | 3 cut(s) | 168, 393, 410 |
| TatI | WGTACW | 1 cut(s) | 340 |
| TauI | GCSGC | 2 cut(s) | 264, 422 |
| TfiI | GAWTC | 1 cut(s) | 688 |
| Tru1I | TTAA | 1 cut(s) | 716 |
| Tru9I | TTAA | 1 cut(s) | 716 |
| TscAI | CASTG | 3 cut(s) | 85, 153, 585 |
| TseFI | GTSAC | 2 cut(s) | 576, 929 |
| TseI | GCWGC | 2 cut(s) | 467, 502 |
| Tsp45I | GTSAC | 2 cut(s) | 576, 929 |
| TspRI | CASTG | 3 cut(s) | 85, 153, 585 |
| Tth111I | GACNNNGTC | 1 cut(s) | 725 |
| VpaK11BI | GGWCC | 1 cut(s) | 306 |
| XapI | RAATTY | 1 cut(s) | 410 |
| XbaI | TCTAGA | 1 cut(s) | 520 |
| XceI | RCATGY | 1 cut(s) | 449 |
| XmiI | GTMKAC | 2 cut(s) | 189, 849 |
| XmnI | GAANNNNTTC | 2 cut(s) | 495, 516 |
| XspI | CTAG | 2 cut(s) | 272, 521 |
| Zsp2I | ATGCAT | 1 cut(s) | 483 |