AT4G22530

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001059
Physical Location & Seq
Reverse (-)
11858444 .. 11860426
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22530.1

Sequence Viewer

Length: 786 bp
ATGTCAGGAGTTTACGATAGTCAAGCTGATATCTACTTAGACGCACGACCTACTTATCCCGCCGATTGGTACTCCAAGCTCGCCGCTCTCTCCCACCGTCACAATCTCGCTTGGGACGCCGGAACCGGAAACGGCCAAGCTGCCATCGGAATCGCGGAACACTACGAAAGAGTCGTTGCGACGGACGTTAGCGAGACGATGTTGAATCTCGGAAAACCGCATCCGAAAGTAACGTACCACCACACGCCCTCGTCGATGACGGAAGATGAAATGGTAGATCTTATTGGAGGAGAGAACTCTGTGGATTTGATAACGGTGGCTACCGCCGTACACTGGTTCGATCTCCCGAGATTCTACGCGATCGCGAATCGTCTCCTCCGTAAACCAGGAGGAATCATCGCCGTGTGGAGCTACAACACTGACATGGTGGTGAATCCCGAGTTCGACTCGGTGATGACTCGGTTCATCGCTGAAACGTTGCCGTATTGTAAATTCCCAGAGAGTCAGTATTTTTTGGACGGATATAAGACGTTGCCGTTTCCGTTTGAGAGCGTGGGTTTGGGCTCAGAGGGAAAGCCCATGGAACTGGAGATGAAGAAAACGGTGTCGTTTGAAGGATTCTTGCGGATGCTAAGATCGTGGTCTGCCGTTGGTGCTGCTAAAGAAAAAGGAGTAGACTTATTGTCGGACAATGTAGTCAAAGAGCTCGAGACGGCTTGGGGTGGTTATGAGCTGGTTCGAACCATTGTTTACAAGACGTTTATGCTCGCAGGAACCGTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

261

Amino Acids

29.11

Weight (kDa)

5.17

Isoelectric Point (pI)

29.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 39 - 134 2.8e-09 Methyltransferase domain
Methyltransf_25 PF13649 39 - 130 8.3e-09 Methyltransferase domain
Methyltransf_12 PF08242 39 - 132 4.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 695
AccBSI CCGCTC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 675
AccII CGCG 3 cut(s) 155, 359, 365
AciI CCGC 6 cut(s) 60, 84, 155, 218, 324, 625
AclI AACGTT 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 3 cut(s) 71, 236, 330
AfiI CCNNNNNNNGG 2 cut(s) 66, 333
AgsI TTSAA 2 cut(s) 205, 614
AhdI GACNNNNNGTC 1 cut(s) 682
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 6 cut(s) 26, 79, 140, 411, 706, 733
AluI AGCT 6 cut(s) 26, 79, 140, 411, 706, 733
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 3 cut(s) 188, 377, 704
Ama87I CYCGRG 3 cut(s) 346, 437, 707
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 2 cut(s) 140, 656
ApoI RAATTY 1 cut(s) 491
AsiSI GCGATCGC 1 cut(s) 363
AsuHPI GGTGA 2 cut(s) 442, 463
AsuII TTCGAA 1 cut(s) 739
AvaI CYCGRG 3 cut(s) 346, 437, 707
BanII GRGCYC 2 cut(s) 566, 708
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 2 cut(s) 127, 643
BccI CCATC 1 cut(s) 152
BceAI ACGGC 7 cut(s) 148, 311, 386, 466, 520, 632, 729
BciT130I CCWGG 1 cut(s) 387
BcoDI GTCTC 3 cut(s) 188, 377, 704
BglII AGATCT 1 cut(s) 277
BisI GCNGC 3 cut(s) 84, 141, 657
BlsI GCNGC 3 cut(s) 85, 142, 658
Bme1390I CCNGG 1 cut(s) 387
BmeRI GACNNNNNGTC 1 cut(s) 682
BmeT110I CYCGRG 3 cut(s) 346, 437, 707
BmiI GGNNCC 2 cut(s) 124, 775
BmrFI CCNGG 1 cut(s) 387
BmsI GCATC 2 cut(s) 229, 618
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 608
Bpu14I TTCGAA 1 cut(s) 739
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 579
BsaWI WCCGGW 1 cut(s) 125
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 333
Bse1I ACTGG 2 cut(s) 338, 591
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 2 cut(s) 220, 633
BseLI CCNNNNNNNGG 2 cut(s) 66, 333
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 2 cut(s) 338, 591
BseRI GAGGAG 2 cut(s) 303, 365
BseXI GCAGC 2 cut(s) 127, 643
Bsh1236I CGCG 3 cut(s) 155, 359, 365
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 3 cut(s) 346, 437, 707
BsiSI CCGG 2 cut(s) 120, 126
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 66, 333
BsmAI GTCTC 3 cut(s) 188, 377, 704
BsmBI CGTCTC 3 cut(s) 188, 377, 704
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 135
BsoBI CYCGRG 3 cut(s) 346, 437, 707
Bsp119I TTCGAA 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 566, 708
Bsp143I GATC 4 cut(s) 277, 340, 360, 635
Bsp19I CCATGG 1 cut(s) 579
Bsp68I TCGCGA 1 cut(s) 365
BspACI CCGC 6 cut(s) 60, 84, 155, 218, 324, 625
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 1 cut(s) 578
BspFNI CGCG 3 cut(s) 155, 359, 365
BspLI GGNNCC 2 cut(s) 124, 775
BspT104I TTCGAA 1 cut(s) 739
BsrBI CCGCTC 1 cut(s) 86
BsrI ACTGG 2 cut(s) 338, 591
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 4 cut(s) 277, 340, 360, 635
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 4 cut(s) 98, 316, 604, 778
BstACI GRCGYC 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 739
BstC8I GCNNGC 2 cut(s) 81, 768
BstDEI CTNAG 4 cut(s) 37, 565, 632, 783
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 220, 633
BstFNI CGCG 3 cut(s) 155, 359, 365
BstKTI GATC 4 cut(s) 280, 343, 363, 638
BstMAI GTCTC 3 cut(s) 188, 377, 704
BstMBI GATC 4 cut(s) 277, 340, 360, 635
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 2 cut(s) 116, 653
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 385
BstUI CGCG 3 cut(s) 155, 359, 365
BstV1I GCAGC 2 cut(s) 127, 643
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 579
BtgZI GCGATG 2 cut(s) 382, 451
BtsCI GGATG 2 cut(s) 220, 633
BtsIMutI CAGTG 2 cut(s) 331, 417
BtuMI TCGCGA 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 81, 768
CseI GACGC 2 cut(s) 50, 125
Csp6I GTAC 3 cut(s) 70, 235, 329
CviAII CATG 2 cut(s) 424, 580
CviQI GTAC 3 cut(s) 70, 235, 329
DdeI CTNAG 4 cut(s) 37, 565, 632, 783
DpnI GATC 4 cut(s) 279, 342, 362, 637
DpnII GATC 4 cut(s) 277, 340, 360, 635
DrdI GACNNNNNNGTC 1 cut(s) 695
DriI GACNNNNNGTC 1 cut(s) 682
DseDI GACNNNNNNGTC 1 cut(s) 695
EaeI YGGCCR 1 cut(s) 133
Eam1105I GACNNNNNGTC 1 cut(s) 682
Ecl136II GAGCTC 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 579
Eco24I GRGCYC 2 cut(s) 566, 708
Eco32I GATATC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 706
Eco88I CYCGRG 3 cut(s) 346, 437, 707
EcoICRI GAGCTC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 579
EcoT38I GRGCYC 2 cut(s) 566, 708
ErhI CCWWGG 1 cut(s) 579
Esp3I CGTCTC 3 cut(s) 188, 377, 704
FaeI CATG 2 cut(s) 427, 583
FaiI YATR 5 cut(s) 425, 525, 581, 729, 764
FaqI GGGAC 1 cut(s) 128
FatI CATG 2 cut(s) 423, 579
FauI CCCGC 1 cut(s) 67
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 3 cut(s) 84, 141, 657
FokI GGATG 2 cut(s) 207, 640
FriOI GRGCYC 2 cut(s) 566, 708
Fsp4HI GCNGC 3 cut(s) 84, 141, 657
GluI GCNGC 3 cut(s) 84, 141, 657
GsuI CTGGAG 1 cut(s) 608
HaeIII GGCC 1 cut(s) 135
HapII CCGG 2 cut(s) 120, 126
HgaI GACGC 2 cut(s) 50, 125
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 2 cut(s) 427, 583
HpaII CCGG 2 cut(s) 120, 126
HphI GGTGA 2 cut(s) 442, 463
Hpy166II GTNNAC 5 cut(s) 13, 331, 383, 676, 751
Hpy188I TCNGA 5 cut(s) 149, 212, 225, 568, 688
Hpy188III TCNNGA 5 cut(s) 6, 346, 364, 437, 709
Hpy8I GTNNAC 5 cut(s) 13, 331, 383, 676, 751
Hpy99I CGWCG 2 cut(s) 184, 256
HpyAV CCTTC 1 cut(s) 608
HpyCH4III ACNGT 4 cut(s) 98, 316, 604, 778
HpyCH4IV ACGT 5 cut(s) 186, 233, 476, 530, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 653
HpyF3I CTNAG 4 cut(s) 37, 565, 632, 783
HpySE526I ACGT 5 cut(s) 186, 233, 476, 530, 758
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 2 cut(s) 427, 583
Kzo9I GATC 4 cut(s) 277, 340, 360, 635
LmnI GCTCC 1 cut(s) 408
LpnPI CCDG 9 cut(s) 133, 139, 319, 372, 399, 510, 572, 719, 756
Lsp1109I GCAGC 2 cut(s) 127, 643
LweI GCATC 2 cut(s) 229, 618
MaeII ACGT 5 cut(s) 186, 233, 476, 530, 758
MaeIII GTNAC 2 cut(s) 98, 229
MalI GATC 4 cut(s) 279, 342, 362, 637
MbiI CCGCTC 1 cut(s) 86
MboI GATC 4 cut(s) 277, 340, 360, 635
MboII GAAGA 2 cut(s) 275, 607
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 566, 708
MluCI AATT 1 cut(s) 491
MlyI GAGTC 4 cut(s) 180, 440, 451, 511
MmeI TCCRAC 1 cut(s) 666
MnlI CCTC 5 cut(s) 259, 281, 383, 386, 562
MslI CAYNNNNRTG 3 cut(s) 401, 422, 428
MspI CCGG 2 cut(s) 120, 126
MspR9I CCNGG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 3 cut(s) 155, 359, 365
MwoI GCNNNNNNNGC 2 cut(s) 116, 653
NcoI CCATGG 1 cut(s) 579
NdeII GATC 4 cut(s) 277, 340, 360, 635
NlaIII CATG 2 cut(s) 427, 583
NlaIV GGNNCC 2 cut(s) 124, 775
NmuCI GTSAC 1 cut(s) 98
NruI TCGCGA 1 cut(s) 365
NspV TTCGAA 1 cut(s) 739
PaeR7I CTCGAG 1 cut(s) 707
PcsI WCGNNNNNNNCGW 4 cut(s) 171, 251, 376, 774
PfeI GAWTC 7 cut(s) 150, 205, 351, 367, 393, 433, 618
PkrI GCNGC 3 cut(s) 85, 142, 658
Ple19I CGATCG 1 cut(s) 363
PleI GAGTC 4 cut(s) 179, 440, 451, 510
PpsI GAGTC 4 cut(s) 179, 440, 451, 510
Psp124BI GAGCTC 1 cut(s) 708
Psp1406I AACGTT 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 124, 775
PsuI RGATCY 1 cut(s) 277
PvuI CGATCG 1 cut(s) 363
RgaI GCGATCGC 1 cut(s) 363
RruI TCGCGA 1 cut(s) 365
RsaI GTAC 3 cut(s) 71, 236, 330
RsaNI GTAC 3 cut(s) 70, 235, 329
RseI CAYNNNNRTG 3 cut(s) 401, 422, 428
SacI GAGCTC 1 cut(s) 708
SatI GCNGC 3 cut(s) 84, 141, 657
Sau3AI GATC 4 cut(s) 277, 340, 360, 635
SchI GAGTC 4 cut(s) 180, 440, 451, 511
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 2 cut(s) 566, 708
SfaAI GCGATCGC 1 cut(s) 363
SfaNI GCATC 2 cut(s) 229, 618
Sfr274I CTCGAG 1 cut(s) 707
SfuI TTCGAA 1 cut(s) 739
SgfI GCGATCGC 1 cut(s) 363
SlaI CTCGAG 1 cut(s) 707
SmiMI CAYNNNNRTG 3 cut(s) 401, 422, 428
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 1 cut(s) 491
SsiI CCGC 6 cut(s) 60, 84, 155, 218, 324, 625
SstI GAGCTC 1 cut(s) 708
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 4 cut(s) 98, 316, 604, 778
TaiI ACGT 5 cut(s) 189, 236, 479, 533, 761
TaqI TCGA 5 cut(s) 254, 339, 444, 708, 739
TasI AATT 1 cut(s) 491
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 7 cut(s) 150, 205, 351, 367, 393, 433, 618
TscAI CASTG 2 cut(s) 338, 424
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 2 cut(s) 140, 656
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 282, 454, 608
TspGWI ACGGA 5 cut(s) 197, 275, 368, 531, 534
TspRI CASTG 2 cut(s) 338, 424
XapI RAATTY 1 cut(s) 491
XhoI CTCGAG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.