Rorug07G0291200

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001059
Physical Location & Seq
Reverse (-)
28397397 .. 28397741
345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0291200.1

Sequence Viewer

Length: 345 bp
ATGACAGGTGCCCTGAGAATGCTTGCGTACGGTGCAGCTGCTGATCAATGTGATGAAATCACTAGAATGGGAGCTTCTACAGCGCTGAAATGTCTGAAGAAGTTCTGTAGACAAGTCGAGTTTCTTTACGCTGGGTGGTTCCTTCGTCCTCAAAATCCTGCTGACTTACATAGGCTTCTCAATAGAGGACAACGCCGTGGGTTTCCAGGCATGATTGGGAGCATCGTTTGCATGCATTGGGAGTGGAAGAATTGCCCAACTGGCTGGGCTGGAGCTTTCTCGGGTCGAAAGGGTAGACCAACTATTATTCTTGAAGCAGTGGCGTCTTACGATACGCATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

114

Amino Acids

12.76

Weight (kDa)

9.55

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_tran PF04827 48 - 114 1.4e-24 Plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccI GTMKAC 2 cut(s) 109, 295
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 323
AfaI GTAC 1 cut(s) 29
AfeI AGCGCT 1 cut(s) 84
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 3 cut(s) 38, 74, 275
AluI AGCT 3 cut(s) 38, 74, 275
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 280
Aor51HI AGCGCT 1 cut(s) 84
ApeKI GCWGC 2 cut(s) 35, 38
Asp700I GAANNNNTTC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 280
BaeGI GKGCMC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 25, 47
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 1 cut(s) 207
BclI TGATCA 1 cut(s) 43
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 2 cut(s) 78, 106
BfoI RGCGCY 1 cut(s) 86
BglI GCCNNNNNGGC 1 cut(s) 261
BisI GCNGC 2 cut(s) 36, 39
BlsI GCNGC 2 cut(s) 37, 40
Bme1390I CCNGG 1 cut(s) 207
BmeT110I CYCGRG 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 10, 140
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 265
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 5
BseNI ACTGG 1 cut(s) 265
BseSI GKGCMC 1 cut(s) 13
BseXI GCAGC 2 cut(s) 25, 47
BseYI CCCAGC 2 cut(s) 131, 264
BsgI GTGCAG 1 cut(s) 54
BshNI GGYRCC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 280
BsiWI CGTACG 1 cut(s) 27
BsmI GAATGC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 6
BspLI GGNNCC 2 cut(s) 10, 140
BspT107I GGYRCC 1 cut(s) 8
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 1 cut(s) 43
BssNI GRCGYC 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 24, 233
BstDEI CTNAG 1 cut(s) 14
BstDSI CCRYGG 1 cut(s) 196
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 46
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 4 cut(s) 32, 80, 228, 261
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 2 cut(s) 78, 106
BstSLI GKGCMC 1 cut(s) 13
BstV1I GCAGC 2 cut(s) 25, 47
BstXI CCANNNNNNTGG 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 196
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 24, 233
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 1 cut(s) 85
CseI GACGC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 211, 232
CviJI RGCY 6 cut(s) 38, 74, 175, 264, 269, 275
CviKI_1 RGCY 6 cut(s) 38, 74, 175, 264, 269, 275
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 14
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
Eco47III AGCGCT 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 237
FaeI CATG 2 cut(s) 214, 235
FaiI YATR 6 cut(s) 171, 212, 233, 339, 341, 343
FatI CATG 2 cut(s) 210, 231
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 2 cut(s) 109, 295
Fnu4HI GCNGC 2 cut(s) 36, 39
Fsp4HI GCNGC 2 cut(s) 36, 39
FspBI CTAG 1 cut(s) 63
GlaI GCGC 1 cut(s) 84
GluI GCNGC 2 cut(s) 36, 39
GsaI CCCAGC 2 cut(s) 135, 268
GsuI CTGGAG 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 86
HgaI GACGC 1 cut(s) 312
HhaI GCGC 1 cut(s) 85
Hin1I GRCGYC 1 cut(s) 323
Hin1II CATG 2 cut(s) 214, 235
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
Hpy166II GTNNAC 2 cut(s) 110, 296
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 110, 296
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4V TGCA 3 cut(s) 35, 231, 235
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 80, 228, 261
HpyF3I CTNAG 1 cut(s) 14
Hsp92I GRCGYC 1 cut(s) 323
Hsp92II CATG 2 cut(s) 214, 235
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 1 cut(s) 43
LmnI GCTCC 3 cut(s) 71, 219, 272
LpnPI CCDG 8 cut(s) 26, 117, 171, 192, 219, 246, 250, 255
Lsp1109I GCAGC 2 cut(s) 25, 47
LweI GCATC 1 cut(s) 231
MaeI CTAG 1 cut(s) 63
MalI GATC 1 cut(s) 45
MboI GATC 1 cut(s) 43
MboII GAAGA 2 cut(s) 109, 259
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 1 cut(s) 250
MnlI CCTC 2 cut(s) 159, 179
Mph1103I ATGCAT 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 101
MslI CAYNNNNRTG 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 4 cut(s) 32, 80, 228, 261
NdeII GATC 1 cut(s) 43
NlaIII CATG 2 cut(s) 214, 235
NlaIV GGNNCC 2 cut(s) 10, 140
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 1 cut(s) 235
PaeI GCATGC 1 cut(s) 235
PctI GAATGC 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 101
Pfl23II CGTACG 1 cut(s) 27
PkrI GCNGC 2 cut(s) 37, 40
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 2 cut(s) 131, 264
PspGI CCWGG 1 cut(s) 205
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 10, 140
PstNI CAGNNNCTG 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 1 cut(s) 65
SatI GCNGC 2 cut(s) 36, 39
Sau3AI GATC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 13
SetI ASST 4 cut(s) 10, 40, 76, 277
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 2 cut(s) 78, 106
SmiMI CAYNNNNRTG 1 cut(s) 65
SphI GCATGC 1 cut(s) 235
Sse9I AATT 1 cut(s) 250
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 32
TaqI TCGA 2 cut(s) 117, 286
TasI AATT 1 cut(s) 250
TscAI CASTG 1 cut(s) 324
TseI GCWGC 2 cut(s) 35, 38
TspDTI ATGAA 1 cut(s) 69
TspRI CASTG 1 cut(s) 324
XceI RCATGY 1 cut(s) 235
XmiI GTMKAC 2 cut(s) 109, 295
XmnI GAANNNNTTC 1 cut(s) 101
XspI CTAG 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.