RchiOBHm_Chr7g0235451

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001059
Physical Location & Seq
Forward (+)
60190067 .. 60191224
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21103

Sequence Viewer

Length: 825 bp
ATGTTAGTTTCATTTGGTACTTTTGCTATGATGCATGTGGAAGTGACAGGTCTATTTGACAAGCAGGCAGAGATATACGTAAAAGGTCGGCCAACATATCCCAAGGAATGGTATTCTATGCTGGCTGCTCTCACCCCACAACACTCTTTAGCTTGGGATGTTGGCACTGGCAATGGCCAAGCTACCGTTGGTCTTTTAGAGCATTACGACCAAGTAGTTGCAAGTGACATAAGTGACGCACAATTGAAATATGCCATCCAACACCCTCGAGTTCGATACATTCACACTCCAGCATCAATCTCAGACGATGAAATGGTAAACTTAATAGGGGGTGGTCAAGAAAATTCAGTTGATTTGGTTACAGTGGCTGCTGCCATTCACTGGTTTGATCTCCCACGCTTCTACTCGCTTGTCAAACGCCTTCTGCGACAACCAGGAGGCATAATCGCTGTTTGGTGCTACAATGGCATGGTCGTAAGCCCCGACTTTGATCTTGTAATGAAGCGTTTACACGAAAGTTGTCTACCATTTTGGGACCCGAGGACAAAGTACGCATGGGGGGGTTATACGACACTTCCTTTTCCATTTGAGAGCGTTGGATTGGGTCGTGAAGGTGAACCTATCACACTTGAAATACCAAAGGAATTGTCATTTCAGGGGTTTGAGAGGATGTTGAGGTCTTTCTCTGCAGTCGCTACAGCTAAAGACCGGGGTGCGGATTTGTTGTCTGAAGATGTGATTAAAGAGCTTCACAGAGCTTGGGGTGCGCATGATCTGGTTAGAACAGTTACATACAAGGCATTTATGCTTGTGGGCAGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

274

Amino Acids

30.79

Weight (kDa)

5.98

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 53 - 149 2.2e-09 Methyltransferase domain
Methyltransf_25 PF13649 53 - 146 6e-08 Methyltransferase domain
Methyltransf_12 PF08242 53 - 147 1.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 823
Acc16I TGCGCA 1 cut(s) 768
AccB7I CCANNNNNTGG 2 cut(s) 108, 381
AccI GTMKAC 1 cut(s) 523
AciI CCGC 1 cut(s) 716
AcoI YGGCCR 2 cut(s) 89, 175
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 2 cut(s) 19, 551
AfiI CCNNNNNNNGG 3 cut(s) 108, 381, 715
AgsI TTSAA 2 cut(s) 247, 632
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 5 cut(s) 152, 182, 701, 748, 758
AluI AGCT 5 cut(s) 152, 182, 701, 748, 758
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 2 cut(s) 267, 538
AoxI GGCC 2 cut(s) 89, 175
ApeKI GCWGC 3 cut(s) 125, 368, 371
ApoI RAATTY 1 cut(s) 343
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 1 cut(s) 769
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 2 cut(s) 124, 626
AvaI CYCGRG 2 cut(s) 267, 538
AvaII GGWCC 1 cut(s) 535
BalI TGGCCA 1 cut(s) 177
BbvI GCAGC 3 cut(s) 112, 355, 358
BccI CCATC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 710
BfmI CTRYAG 2 cut(s) 687, 696
BisI GCNGC 3 cut(s) 126, 369, 372
BlsI GCNGC 3 cut(s) 127, 370, 373
Bme1390I CCNGG 2 cut(s) 435, 710
Bme18I GGWCC 1 cut(s) 535
BmeT110I CYCGRG 2 cut(s) 267, 538
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 2 cut(s) 536, 537
BmrFI CCNGG 2 cut(s) 435, 710
BmsI GCATC 2 cut(s) 21, 302
BpmI CTGGAG 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 710
BsaAI YACGTR 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 102, 539, 709
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 381, 715
Bse1I ACTGG 2 cut(s) 172, 386
Bse3DI GCAATG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 3 cut(s) 102, 539, 709
BseGI GGATG 3 cut(s) 163, 255, 675
BseLI CCNNNNNNNGG 3 cut(s) 108, 381, 715
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 2 cut(s) 172, 386
BseXI GCAGC 3 cut(s) 112, 355, 358
BshFI GGCC 2 cut(s) 91, 177
BsiHKCI CYCGRG 2 cut(s) 267, 538
BsiSI CCGG 1 cut(s) 709
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 3 cut(s) 108, 381, 715
BsmFI GGGAC 1 cut(s) 548
BsnI GGCC 2 cut(s) 91, 177
BsoBI CYCGRG 2 cut(s) 267, 538
Bsp143I GATC 3 cut(s) 388, 490, 772
BspACI CCGC 1 cut(s) 716
BspANI GGCC 2 cut(s) 91, 177
BspCNI CTCAG 1 cut(s) 314
BspLI GGNNCC 2 cut(s) 536, 537
BspMAI CTGCAG 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 178
BsrI ACTGG 2 cut(s) 172, 386
BssECI CCNNGG 3 cut(s) 102, 539, 709
BssMI GATC 3 cut(s) 388, 490, 772
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 187, 364, 787
BstBAI YACGTR 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 66, 123
BstDEI CTNAG 1 cut(s) 301
BstF5I GGATG 3 cut(s) 163, 255, 675
BstHHI GCGC 1 cut(s) 769
BstKTI GATC 3 cut(s) 391, 493, 775
BstMBI GATC 3 cut(s) 388, 490, 772
BstMWI GCNNNNNNNGC 2 cut(s) 465, 764
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 433, 708
BstSFI CTRYAG 2 cut(s) 687, 696
BstSNI TACGTA 1 cut(s) 79
BstV1I GCAGC 3 cut(s) 112, 355, 358
BsuRI GGCC 2 cut(s) 91, 177
BtsCI GGATG 3 cut(s) 163, 255, 675
BtsIMutI CAGTG 3 cut(s) 165, 369, 379
Cac8I GCNNGC 2 cut(s) 66, 123
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 1 cut(s) 769
Cfr13I GGNCC 1 cut(s) 535
CseI GACGC 1 cut(s) 245
Csp6I GTAC 2 cut(s) 18, 550
CviAII CATG 4 cut(s) 35, 469, 555, 770
CviQI GTAC 2 cut(s) 18, 550
DdeI CTNAG 1 cut(s) 301
DpnI GATC 3 cut(s) 390, 492, 774
DpnII GATC 3 cut(s) 388, 490, 772
EaeI YGGCCR 2 cut(s) 89, 175
Eco105I TACGTA 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 750
Eco88I CYCGRG 2 cut(s) 267, 538
EcoO109I RGGNCCY 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 4 cut(s) 38, 472, 558, 773
FaqI GGGAC 1 cut(s) 548
FatI CATG 4 cut(s) 34, 468, 554, 769
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 3 cut(s) 126, 369, 372
FokI GGATG 3 cut(s) 170, 242, 682
Fsp4HI GCNGC 3 cut(s) 126, 369, 372
FspAI RTGCGCAY 1 cut(s) 768
FspI TGCGCA 1 cut(s) 768
GlaI GCGC 1 cut(s) 768
GluI GCNGC 3 cut(s) 126, 369, 372
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 91, 177
HapII CCGG 1 cut(s) 709
HgaI GACGC 1 cut(s) 245
HhaI GCGC 1 cut(s) 769
Hin1II CATG 4 cut(s) 38, 472, 558, 773
Hin6I GCGC 1 cut(s) 767
HinP1I GCGC 1 cut(s) 767
HpaII CCGG 1 cut(s) 709
HphI GGTGA 2 cut(s) 124, 626
Hpy166II GTNNAC 4 cut(s) 319, 509, 524, 617
Hpy188I TCNGA 2 cut(s) 304, 730
Hpy188III TCNNGA 2 cut(s) 338, 608
Hpy8I GTNNAC 4 cut(s) 319, 509, 524, 617
HpyAV CCTTC 2 cut(s) 431, 605
HpyCH4III ACNGT 3 cut(s) 187, 364, 787
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 3 cut(s) 34, 221, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 465, 764
HpyF3I CTNAG 1 cut(s) 301
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 4 cut(s) 38, 472, 558, 773
HspAI GCGC 1 cut(s) 767
KflI GGGWCCC 1 cut(s) 535
Kzo9I GATC 3 cut(s) 388, 490, 772
Lsp1109I GCAGC 3 cut(s) 112, 355, 358
LweI GCATC 2 cut(s) 21, 302
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 5 cut(s) 43, 224, 233, 358, 787
MalI GATC 3 cut(s) 390, 492, 774
MboI GATC 3 cut(s) 388, 490, 772
MboII GAAGA 1 cut(s) 743
MfeI CAATTG 1 cut(s) 242
MlsI TGGCCA 1 cut(s) 177
MluCI AATT 3 cut(s) 242, 343, 644
MluNI TGGCCA 1 cut(s) 177
MmeI TCCRAC 2 cut(s) 283, 577
MnlI CCTC 5 cut(s) 276, 431, 534, 660, 669
Mox20I TGGCCA 1 cut(s) 177
Mph1103I ATGCAT 1 cut(s) 36
MscI TGGCCA 1 cut(s) 177
MseI TTAA 2 cut(s) 323, 741
Msp20I TGGCCA 1 cut(s) 177
MspI CCGG 1 cut(s) 709
MspR9I CCNGG 2 cut(s) 435, 710
MunI CAATTG 1 cut(s) 242
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 465, 764
NciI CCSGG 1 cut(s) 710
NdeII GATC 3 cut(s) 388, 490, 772
NlaIII CATG 4 cut(s) 38, 472, 558, 773
NlaIV GGNNCC 2 cut(s) 536, 537
NmuCI GTSAC 3 cut(s) 43, 224, 233
NsbI TGCGCA 1 cut(s) 768
NsiI ATGCAT 1 cut(s) 36
NspI RCATGY 1 cut(s) 38
PaeR7I CTCGAG 1 cut(s) 267
PcsI WCGNNNNNNNCGW 2 cut(s) 424, 480
PflMI CCANNNNNTGG 2 cut(s) 108, 381
PkrI GCNGC 3 cut(s) 127, 370, 373
Ppu21I YACGTR 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 535
PsiI TTATAA 1 cut(s) 823
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 536, 537
PspPI GGNCC 1 cut(s) 535
PspPPI RGGWCCY 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 267
PstI CTGCAG 1 cut(s) 691
PstNI CAGNNNCTG 1 cut(s) 368
RsaI GTAC 2 cut(s) 19, 551
RsaNI GTAC 2 cut(s) 18, 550
SaqAI TTAA 2 cut(s) 323, 741
SatI GCNGC 3 cut(s) 126, 369, 372
Sau3AI GATC 3 cut(s) 388, 490, 772
Sau96I GGNCC 1 cut(s) 535
ScrFI CCNGG 2 cut(s) 435, 710
SfaNI GCATC 2 cut(s) 21, 302
SfcI CTRYAG 2 cut(s) 687, 696
Sfr274I CTCGAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 535
SlaI CTCGAG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
SnaBI TACGTA 1 cut(s) 79
Sse9I AATT 3 cut(s) 242, 343, 644
SsiI CCGC 1 cut(s) 716
StyD4I CCNGG 2 cut(s) 433, 708
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 3 cut(s) 187, 364, 787
TaiI ACGT 1 cut(s) 81
TaqI TCGA 2 cut(s) 268, 274
TasI AATT 3 cut(s) 242, 343, 644
Tru1I TTAA 2 cut(s) 323, 741
Tru9I TTAA 2 cut(s) 323, 741
TscAI CASTG 3 cut(s) 172, 369, 386
TseFI GTSAC 3 cut(s) 43, 224, 233
TseI GCWGC 3 cut(s) 125, 368, 371
Tsp45I GTSAC 3 cut(s) 43, 224, 233
TspDTI ATGAA 2 cut(s) 324, 515
TspRI CASTG 3 cut(s) 172, 369, 386
Van91I CCANNNNNTGG 2 cut(s) 108, 381
VpaK11BI GGWCC 1 cut(s) 535
XapI RAATTY 1 cut(s) 343
XceI RCATGY 1 cut(s) 38
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 523
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.