pycom15g38510

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001059
Physical Location & Seq
Reverse (-)
38285687 .. 38286214
528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g38510.1

Sequence Viewer

Length: 528 bp
ATGACCATGTCCGATGACGAGTTGGTTGACCTAATAGGGGGCGAAAACTCAGTTGATTTAGTGACCGTGGCAGAGGCCGTGCACTGGTTCGACCTCCCAAAGTTCTACTCGCTCGTCAAGCGCGTTTTGCGTAAGCCAGGAGGTGTAATTGCAGTTTGGGGCTACAATGCTTGCGTAACACACGCCGAAATCGAGGATGTACTGAAGCGTTTTTATGAAGCAAGTAAACCGTTTTGGGACCCGAAGAGGGAGTACTTGTGGGAAGGGTATAGGACACTTCCATTTCCATTTGAGAGCATTGGTTTGGGTTCTGAAGGGCAGCCTGTGGCACTGGAAATACCAAAGGAAGTGTCATTTAAAGGGGTGTTGAGGATGTTAAGGTCGTCCTCATCAGTCCATACAGCTAAAGATCAAGGCGTGGATTTGTTATCACACGAGGTGATTGAAGAGATTCAAAGAGCTTGGGGAGGGCCTAACGTGGTCAGAAATGTTACGTTCAAGGCATTTATGCTTGCCGGAAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

176

Amino Acids

19.69

Weight (kDa)

5.61

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 123
AcuI CTGAAG 2 cut(s) 224, 333
AdeI CACNNNGTG 1 cut(s) 439
AfaI GTAC 2 cut(s) 201, 254
AfiI CCNNNNNNNGG 3 cut(s) 37, 84, 247
AgsI TTSAA 3 cut(s) 446, 455, 499
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 2 cut(s) 404, 461
AluI AGCT 2 cut(s) 404, 461
Alw21I GWGCWC 1 cut(s) 84
Alw44I GTGCAC 1 cut(s) 80
AoxI GGCC 2 cut(s) 75, 470
ApaLI GTGCAC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 319
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 238, 470
AsuHPI GGTGA 1 cut(s) 451
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 84
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
BauI CACGAG 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 331
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 138
BisI GCNGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 321
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 238, 470
BmiI GGNNCC 2 cut(s) 239, 240
BmrFI CCNGG 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 66
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 84, 247
Bse1I ACTGG 2 cut(s) 89, 336
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 2 cut(s) 202, 378
BseLI CCNNNNNNNGG 3 cut(s) 37, 84, 247
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 89, 336
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 123
BshFI GGCC 2 cut(s) 77, 472
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 516
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 3 cut(s) 37, 84, 247
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 2 cut(s) 77, 472
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 409
BspANI GGCC 2 cut(s) 77, 472
BspCNI CTCAG 1 cut(s) 62
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 239, 240
BsrI ACTGG 2 cut(s) 89, 336
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 409
BssSI CACGAG 1 cut(s) 434
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 67, 231
Bst6I CTCTTC 2 cut(s) 239, 441
BstC8I GCNNGC 2 cut(s) 172, 513
BstDEI CTNAG 1 cut(s) 49
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 202, 378
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 1 cut(s) 412
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 2 cut(s) 118, 127
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 331
BsuRI GGCC 2 cut(s) 77, 472
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 202, 378
BtsIMutI CAGTG 2 cut(s) 82, 329
Cac8I GCNNGC 2 cut(s) 172, 513
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 2 cut(s) 238, 470
Csp6I GTAC 2 cut(s) 200, 253
CviAII CATG 1 cut(s) 7
CviJI RGCY 7 cut(s) 77, 136, 162, 322, 404, 461, 472
CviKI_1 RGCY 7 cut(s) 77, 136, 162, 322, 404, 461, 472
CviQI GTAC 2 cut(s) 200, 253
DdeI CTNAG 1 cut(s) 49
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
DraI TTTAAA 1 cut(s) 358
DraIII CACNNNGTG 1 cut(s) 439
Eam1104I CTCTTC 2 cut(s) 239, 441
EarI CTCTTC 2 cut(s) 239, 441
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 224, 333
EcoO109I RGGNCCY 2 cut(s) 238, 470
EcoRII CCWGG 1 cut(s) 136
FaeI CATG 1 cut(s) 10
FaiI YATR 5 cut(s) 8, 216, 270, 399, 509
FaqI GGGAC 1 cut(s) 251
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 320
FokI GGATG 2 cut(s) 209, 385
Fsp4HI GCNGC 1 cut(s) 320
GlaI GCGC 1 cut(s) 122
GluI GCNGC 1 cut(s) 320
HaeIII GGCC 2 cut(s) 77, 472
HapII CCGG 1 cut(s) 516
HhaI GCGC 1 cut(s) 123
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 1 cut(s) 451
HpaII CCGG 1 cut(s) 516
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 3 cut(s) 28, 82, 227
Hpy188I TCNGA 3 cut(s) 13, 313, 485
Hpy8I GTNNAC 3 cut(s) 28, 82, 227
HpyAV CCTTC 2 cut(s) 257, 308
HpyCH4III ACNGT 2 cut(s) 67, 231
HpyCH4IV ACGT 2 cut(s) 477, 494
HpyCH4V TGCA 2 cut(s) 82, 152
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 127
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 2 cut(s) 477, 494
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 1 cut(s) 121
KflI GGGWCCC 1 cut(s) 238
Kzo9I GATC 1 cut(s) 409
LpnPI CCDG 5 cut(s) 70, 123, 150, 317, 336
Lsp1109I GCAGC 1 cut(s) 331
MaeII ACGT 2 cut(s) 477, 494
MaeIII GTNAC 3 cut(s) 61, 175, 490
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 2 cut(s) 256, 458
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 1 cut(s) 147
MnlI CCTC 9 cut(s) 67, 104, 134, 187, 240, 363, 397, 430, 461
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 2 cut(s) 357, 377
MspI CCGG 1 cut(s) 516
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 118, 127
NdeII GATC 1 cut(s) 409
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 2 cut(s) 239, 240
NmuCI GTSAC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 189
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 1 cut(s) 451
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 321
PpuMI RGGWCCY 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 2 cut(s) 239, 240
PspPI GGNCC 2 cut(s) 238, 470
PspPPI RGGWCCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 201, 254
RsaNI GTAC 2 cut(s) 200, 253
SaqAI TTAA 2 cut(s) 357, 377
SatI GCNGC 1 cut(s) 320
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 2 cut(s) 238, 470
ScaI AGTACT 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 84
SetI ASST 9 cut(s) 33, 96, 145, 383, 406, 441, 463, 480, 497
SinI GGWCC 1 cut(s) 238
Sse9I AATT 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 2 cut(s) 67, 231
TaiI ACGT 2 cut(s) 480, 497
TaqI TCGA 2 cut(s) 90, 192
TasI AATT 1 cut(s) 147
TatI WGTACW 2 cut(s) 199, 252
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 2 cut(s) 357, 377
Tru9I TTAA 2 cut(s) 357, 377
TscAI CASTG 2 cut(s) 89, 336
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 231
TspRI CASTG 2 cut(s) 89, 336
Tth111I GACNNNGTC 1 cut(s) 7
VneI GTGCAC 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 450
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.