Rroxscaffold_3G00225980

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001059
Physical Location & Seq
Reverse (-)
9386785 .. 9387306
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225980.1

Sequence Viewer

Length: 522 bp
ATGGTTAACTTAATAGGGGGCGGTCATGAAAATTCAGTTGATTTGGTGACAGTGGCTTCTGCCGTTCACTGGTTTGATCTGTCACACTTCTACTCGCTTGTCAAACGCCTTCTGCGTAAACCAGGAGGCATAATTGCTGTTTGGTGCTACAACAGCATGGACTGCACGGCCGTAGGCCCTGACTTTGATCTTCTAATGAAGCGTTTACACGAAAATTGTCTACCATTTCGGGACCCGAGGGCAAAGTACGCATTTGAGGGTTATAGGACACTTCCTTTTCCATTTGAGAGCGTTGGATTGGGTCGTGAAGGTGAACCTATCACACTTGAAATACCGAGGGAATTGTCATTTCAGGGGGTTGAGAGGATGTTGAGGTCTTACTCTGCAGTCACTACGGCTAAAGACCAGGGTGTGGATTTGTTGTCTGAAGATGTGATTAAAGAGCTTCAAAGAGCTTGGGGTGGGCATGATCTGATTAGATCAGTTACAATCAAGGCATTTATGCTTGTGGGCAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

173

Amino Acids

19.46

Weight (kDa)

6.51

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 520
AccB7I CCANNNNNTGG 1 cut(s) 412
AccI GTMKAC 1 cut(s) 220
AciI CCGC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 69, 412
AgsI TTSAA 2 cut(s) 329, 449
AjnI CCWGG 2 cut(s) 121, 405
AluBI AGCT 2 cut(s) 445, 455
AluI AGCT 2 cut(s) 445, 455
Ama87I CYCGRG 1 cut(s) 235
AoxI GGCC 2 cut(s) 168, 175
ApoI RAATTY 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 176, 232
AsuHPI GGTGA 2 cut(s) 58, 323
AvaI CYCGRG 1 cut(s) 235
AvaII GGWCC 1 cut(s) 232
BceAI ACGGC 4 cut(s) 47, 155, 183, 411
BciT130I CCWGG 2 cut(s) 123, 407
BfmI CTRYAG 1 cut(s) 384
Bme1390I CCNGG 2 cut(s) 123, 407
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 176, 232
BmiI GGNNCC 2 cut(s) 233, 234
BmrFI CCNGG 2 cut(s) 123, 407
BsaJI CCNNGG 3 cut(s) 236, 335, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 412
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 2 cut(s) 123, 407
BseDI CCNNGG 3 cut(s) 236, 335, 406
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 2 cut(s) 69, 412
BseNI ACTGG 1 cut(s) 74
BseX3I CGGCCG 1 cut(s) 168
BsgI GTGCAG 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 170, 177
BsiEI CGRYCG 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 235
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 2 cut(s) 69, 412
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 2 cut(s) 170, 177
BsoBI CYCGRG 1 cut(s) 235
Bsp143I GATC 4 cut(s) 76, 187, 469, 479
BspACI CCGC 1 cut(s) 21
BspANI GGCC 2 cut(s) 170, 177
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 233, 234
BspMAI CTGCAG 1 cut(s) 388
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 236, 335, 406
BssMI GATC 4 cut(s) 76, 187, 469, 479
Bst2UI CCWGG 2 cut(s) 123, 407
Bst4CI ACNGT 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 162
BstF5I GGATG 1 cut(s) 372
BstKTI GATC 4 cut(s) 79, 190, 472, 482
BstMBI GATC 4 cut(s) 76, 187, 469, 479
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 3 cut(s) 153, 162, 248
BstNI CCWGG 2 cut(s) 123, 407
BstSCI CCNGG 2 cut(s) 121, 405
BstSFI CTRYAG 1 cut(s) 384
BstZI CGGCCG 1 cut(s) 168
BsuRI GGCC 2 cut(s) 170, 177
BtsCI GGATG 1 cut(s) 372
BtsIMutI CAGTG 2 cut(s) 57, 67
CciI TCATGA 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 176, 232
Csp6I GTAC 1 cut(s) 247
CviAII CATG 3 cut(s) 26, 157, 467
CviJI RGCY 6 cut(s) 56, 170, 177, 398, 445, 455
CviKI_1 RGCY 6 cut(s) 56, 170, 177, 398, 445, 455
CviQI GTAC 1 cut(s) 247
DpnI GATC 4 cut(s) 78, 189, 471, 481
DpnII GATC 4 cut(s) 76, 187, 469, 479
EaeI YGGCCR 1 cut(s) 168
EagI CGGCCG 1 cut(s) 168
EclXI CGGCCG 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 232
Eco52I CGGCCG 1 cut(s) 168
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 235
EcoO109I RGGNCCY 2 cut(s) 176, 232
EcoRII CCWGG 2 cut(s) 121, 405
FaeI CATG 3 cut(s) 29, 160, 470
FaiI YATR 7 cut(s) 27, 131, 158, 264, 468, 503, 520
FaqI GGGAC 1 cut(s) 245
FatI CATG 3 cut(s) 25, 156, 466
FblI GTMKAC 1 cut(s) 220
FokI GGATG 1 cut(s) 379
HaeIII GGCC 2 cut(s) 170, 177
Hin1II CATG 3 cut(s) 29, 160, 470
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HpaI GTTAAC 1 cut(s) 7
HphI GGTGA 2 cut(s) 58, 323
Hpy166II GTNNAC 6 cut(s) 7, 67, 119, 206, 221, 314
Hpy188I TCNGA 2 cut(s) 427, 474
Hpy188III TCNNGA 3 cut(s) 26, 230, 305
Hpy8I GTNNAC 6 cut(s) 7, 67, 119, 206, 221, 314
HpyAV CCTTC 2 cut(s) 119, 302
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 2 cut(s) 165, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 162, 248
Hsp92II CATG 3 cut(s) 29, 160, 470
KflI GGGWCCC 1 cut(s) 232
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 4 cut(s) 76, 187, 469, 479
LpnPI CCDG 7 cut(s) 55, 108, 135, 192, 338, 392, 419
MaeIII GTNAC 4 cut(s) 46, 81, 388, 484
MalI GATC 4 cut(s) 78, 189, 471, 481
MboI GATC 4 cut(s) 76, 187, 469, 479
MboII GAAGA 2 cut(s) 182, 440
MluCI AATT 5 cut(s) 31, 132, 214, 341, 515
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 6 cut(s) 119, 231, 250, 330, 357, 366
MseI TTAA 3 cut(s) 6, 11, 438
MspR9I CCNGG 2 cut(s) 123, 407
MvaI CCWGG 2 cut(s) 123, 407
MwoI GCNNNNNNNGC 3 cut(s) 153, 162, 248
NdeII GATC 4 cut(s) 76, 187, 469, 479
NlaIII CATG 3 cut(s) 29, 160, 470
NlaIV GGNNCC 2 cut(s) 233, 234
NmuCI GTSAC 3 cut(s) 46, 81, 388
PagI TCATGA 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 412
PpuMI RGGWCCY 1 cut(s) 232
PsiI TTATAA 1 cut(s) 520
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 121, 405
PspGI CCWGG 2 cut(s) 121, 405
PspN4I GGNNCC 2 cut(s) 233, 234
PspPI GGNCC 2 cut(s) 176, 232
PspPPI RGGWCCY 1 cut(s) 232
PstI CTGCAG 1 cut(s) 388
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SaqAI TTAA 3 cut(s) 6, 11, 438
Sau3AI GATC 4 cut(s) 76, 187, 469, 479
Sau96I GGNCC 2 cut(s) 176, 232
ScrFI CCNGG 2 cut(s) 123, 407
SetI ASST 5 cut(s) 313, 319, 377, 447, 457
SfcI CTRYAG 1 cut(s) 384
SinI GGWCC 1 cut(s) 232
Sse9I AATT 5 cut(s) 31, 132, 214, 341, 515
SsiI CCGC 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 121, 405
TaaI ACNGT 1 cut(s) 52
TasI AATT 5 cut(s) 31, 132, 214, 341, 515
Tru1I TTAA 3 cut(s) 6, 11, 438
Tru9I TTAA 3 cut(s) 6, 11, 438
TscAI CASTG 2 cut(s) 57, 74
TseFI GTSAC 3 cut(s) 46, 81, 388
Tsp45I GTSAC 3 cut(s) 46, 81, 388
TspDTI ATGAA 2 cut(s) 42, 212
TspRI CASTG 2 cut(s) 57, 74
Van91I CCANNNNNTGG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.