RLG00000029430

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001059
Physical Location & Seq
Reverse (-)
38708258 .. 38710587
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029430

Sequence Viewer

Length: 1101 bp
ATGGCACGTCTATTTGACAAGCAGGCAGAGATATACGCGGAAGCTCGGCCAACGTATCCCAAGGAATGGTATTCAAAGCTGGCTGCTCTCACCCCACAACACTCTTTAGCTTGGGATGCTGGCACTGGCAGTGGCCAAGCTACCGTCGGTCTTTTAGAGCATTACGACCAAGTAGTTGGAAGTGATATAAGTGAAGCACAATTGAAGCATGCCATCCAGCACCCTCGAGTTCGATACGTTCACACTCCAGCATCAATCTCAGACGATGAAATGGTTGACTTAATAGGGGGCGGTCATGAAAATTCAGTTGATTTGATTACAGTGGCTACTGCCGTTCACTGGTTTGATCTGCCACGCTTCTACTCGCTTGTCAAACGCCTTCTGCGTAAACCAGGAGGCATAATTGCTGTTTGGTGCTACAACAGCATGGACTGCATGGTCGTAAGCCCTGACTTTGATCTTGCAATGAAGCGTTTACACGAAAATTGTATACCATTTAGGGACCCGAGGGCAAAGTACGCATTTGAGGGTTATAGGACACTTCCTTTTCCATTTGAGAGCGTTGGATTGGGTCGTGAAGGTGAACCTATCCCACTTGAAATACCAAGGGAATTGTCATTTCAGGGGGTTGAGAGGATGTTGAGGTCTTACTCTGCAGTCACTACTGCCAAAGACCAGGGTGTGGATTTGTTGTCTGAAGATGTGACTAAAGAACTTCAAAGAGCTTGGGGTGGGCATGATCTGATTAGATCAGTTACAATCAAGGCATTGAGGGCAGTCCTGACATGTGTTTTTGGGGGCAGCATTTTGTACAATGGCGGTAGTTCCTCTATATCGAGCTCCTTGGATGACCGGTTGTTTTATGAGTCTGCTCTTGATCAATCTGAGAGGTTCCCAACTTTCCGACGGATACAAATGCAAACTAACCTGCATCGAGTTTCAGAGTTTGCTGTAACGGTTACGCAATCTGACAATGGTGGTGAGGCCAGAGGTGAAGCAAAAGCTGCTATCAAGAAGCTTCGCGAGCTTGACAGTGATACTGAATCAGAAGTTGATGACATTGTTGGTAATAGTGTTTCAACAGCTTTATCAGCTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

367

Amino Acids

40.69

Weight (kDa)

5.54

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 39 - 136 8.7e-09 Methyltransferase domain
Methyltransf_25 PF13649 39 - 132 5.9e-08 Methyltransferase domain
Methyltransf_12 PF08242 39 - 134 7.1e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 936
AccB7I CCANNNNNTGG 2 cut(s) 66, 682
AccI GTMKAC 1 cut(s) 490
AccII CGCG 2 cut(s) 38, 1023
AciI CCGC 3 cut(s) 38, 291, 819
AcoI YGGCCR 2 cut(s) 47, 133
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 717
AfaI GTAC 2 cut(s) 518, 812
AfiI CCNNNNNNNGG 3 cut(s) 66, 339, 682
AflIII ACRYGT 1 cut(s) 785
AgeI ACCGGT 1 cut(s) 852
AgsI TTSAA 5 cut(s) 75, 205, 599, 719, 1080
AjiI CACGTC 1 cut(s) 8
AjnI CCWGG 2 cut(s) 391, 675
Alw21I GWGCWC 1 cut(s) 842
Ama87I CYCGRG 2 cut(s) 225, 505
AoxI GGCC 3 cut(s) 47, 133, 984
ApeKI GCWGC 3 cut(s) 83, 801, 1004
ApoI RAATTY 1 cut(s) 301
AsiGI ACCGGT 1 cut(s) 852
AspS9I GGNCC 1 cut(s) 502
AsuHPI GGTGA 4 cut(s) 82, 593, 992, 1004
AvaI CYCGRG 2 cut(s) 225, 505
AvaII GGWCC 1 cut(s) 502
BalI TGGCCA 1 cut(s) 135
BanII GRGCYC 1 cut(s) 842
Bbv12I GWGCWC 1 cut(s) 842
BbvI GCAGC 3 cut(s) 70, 813, 991
BccI CCATC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 2 cut(s) 393, 677
BciVI GTATCC 2 cut(s) 66, 903
BclI TGATCA 1 cut(s) 877
BfmI CTRYAG 1 cut(s) 654
BfuAI ACCTGC 1 cut(s) 936
BfuI GTATCC 2 cut(s) 66, 903
BisI GCNGC 3 cut(s) 84, 802, 1005
BlsI GCNGC 3 cut(s) 85, 803, 1006
Bme1390I CCNGG 2 cut(s) 393, 677
Bme18I GGWCC 1 cut(s) 502
BmeT110I CYCGRG 2 cut(s) 225, 505
BmgBI CACGTC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 3 cut(s) 503, 504, 893
BmrFI CCNGG 2 cut(s) 393, 677
BmsI GCATC 3 cut(s) 106, 260, 940
BpmI CTGGAG 1 cut(s) 231
BsaJI CCNNGG 5 cut(s) 60, 506, 605, 676, 843
BsaWI WCCGGW 1 cut(s) 852
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 339, 682
Bse118I RCCGGY 1 cut(s) 852
Bse1I ACTGG 2 cut(s) 130, 344
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 2 cut(s) 393, 677
BseDI CCNNGG 5 cut(s) 60, 506, 605, 676, 843
BseGI GGATG 4 cut(s) 121, 213, 642, 853
BseLI CCNNNNNNNGG 3 cut(s) 66, 339, 682
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 2 cut(s) 273, 876
BseNI ACTGG 2 cut(s) 130, 344
BseXI GCAGC 3 cut(s) 70, 813, 991
Bsh1236I CGCG 2 cut(s) 38, 1023
BshFI GGCC 3 cut(s) 49, 135, 986
BshTI ACCGGT 1 cut(s) 852
BsiHKAI GWGCWC 1 cut(s) 842
BsiHKCI CYCGRG 2 cut(s) 225, 505
BsiSI CCGG 1 cut(s) 853
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 66, 339, 682
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 3 cut(s) 49, 135, 986
BsoBI CYCGRG 2 cut(s) 225, 505
Bsp1286I GDGCHC 1 cut(s) 842
Bsp1407I TGTACA 1 cut(s) 810
Bsp143I GATC 5 cut(s) 346, 457, 739, 749, 877
Bsp68I TCGCGA 1 cut(s) 1023
BspACI CCGC 3 cut(s) 38, 291, 819
BspANI GGCC 3 cut(s) 49, 135, 986
BspCNI CTCAG 2 cut(s) 272, 877
BspFNI CGCG 2 cut(s) 38, 1023
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 3 cut(s) 503, 504, 893
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 936
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 1 cut(s) 852
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 2 cut(s) 130, 344
BssAI RCCGGY 1 cut(s) 852
BssECI CCNNGG 5 cut(s) 60, 506, 605, 676, 843
BssMI GATC 5 cut(s) 346, 457, 739, 749, 877
BssNAI GTATAC 1 cut(s) 491
BssT1I CCWWGG 3 cut(s) 60, 605, 843
Bst1107I GTATAC 1 cut(s) 491
Bst2UI CCWGG 2 cut(s) 393, 677
Bst4CI ACNGT 4 cut(s) 145, 322, 958, 1034
BstAPI GCANNNNNTGC 2 cut(s) 432, 1004
BstAUI TGTACA 1 cut(s) 810
BstC8I GCNNGC 5 cut(s) 24, 81, 121, 210, 1025
BstDEI CTNAG 2 cut(s) 259, 885
BstF5I GGATG 4 cut(s) 121, 213, 642, 853
BstFNI CGCG 2 cut(s) 38, 1023
BstKTI GATC 5 cut(s) 349, 460, 742, 752, 880
BstMBI GATC 5 cut(s) 346, 457, 739, 749, 877
BstMWI GCNNNNNNNGC 8 cut(s) 116, 423, 432, 518, 773, 1004, 1024, 1091
BstNI CCWGG 2 cut(s) 393, 677
BstNSI RCATGY 2 cut(s) 212, 789
BstSCI CCNGG 2 cut(s) 391, 675
BstSFI CTRYAG 1 cut(s) 654
BstUI CGCG 2 cut(s) 38, 1023
BstV1I GCAGC 3 cut(s) 70, 813, 991
BstXI CCANNNNNNTGG 1 cut(s) 176
BstZ17I GTATAC 1 cut(s) 491
BsuI GTATCC 2 cut(s) 66, 903
BsuRI GGCC 3 cut(s) 49, 135, 986
BtrI CACGTC 1 cut(s) 8
BtsCI GGATG 4 cut(s) 121, 213, 642, 853
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 5 cut(s) 123, 136, 327, 337, 1039
BtuMI TCGCGA 1 cut(s) 1023
BveI ACCTGC 1 cut(s) 936
Cac8I GCNNGC 5 cut(s) 24, 81, 121, 210, 1025
CciI TCATGA 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 852
Cfr13I GGNCC 1 cut(s) 502
Csp6I GTAC 2 cut(s) 517, 811
CspAI ACCGGT 1 cut(s) 852
CviAII CATG 6 cut(s) 209, 296, 427, 436, 737, 786
CviQI GTAC 2 cut(s) 517, 811
DdeI CTNAG 2 cut(s) 259, 885
DpnI GATC 5 cut(s) 348, 459, 741, 751, 879
DpnII GATC 5 cut(s) 346, 457, 739, 749, 877
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 2 cut(s) 47, 133
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 3 cut(s) 60, 605, 843
Eco24I GRGCYC 1 cut(s) 842
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 840
Eco57I CTGAAG 1 cut(s) 717
Eco88I CYCGRG 2 cut(s) 225, 505
EcoICRI GAGCTC 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 391, 675
EcoT14I CCWWGG 3 cut(s) 60, 605, 843
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 3 cut(s) 60, 605, 843
FaeI CATG 6 cut(s) 212, 299, 430, 439, 740, 789
FaqI GGGAC 1 cut(s) 515
FatI CATG 6 cut(s) 208, 295, 426, 435, 736, 785
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 490
Fnu4HI GCNGC 3 cut(s) 84, 802, 1005
FokI GGATG 4 cut(s) 128, 200, 649, 860
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 3 cut(s) 84, 802, 1005
GluI GCNGC 3 cut(s) 84, 802, 1005
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 3 cut(s) 49, 135, 986
HapII CCGG 1 cut(s) 853
Hin1II CATG 6 cut(s) 212, 299, 430, 439, 740, 789
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 2 cut(s) 866, 1043
HpaII CCGG 1 cut(s) 853
HphI GGTGA 4 cut(s) 82, 593, 992, 1004
Hpy166II GTNNAC 7 cut(s) 241, 277, 337, 389, 476, 491, 584
Hpy188I TCNGA 8 cut(s) 262, 697, 744, 886, 905, 943, 970, 1048
Hpy188III TCNNGA 6 cut(s) 296, 575, 781, 875, 1012, 1022
Hpy8I GTNNAC 7 cut(s) 241, 277, 337, 389, 476, 491, 584
Hpy99I CGWCG 2 cut(s) 149, 909
HpyAV CCTTC 2 cut(s) 389, 572
HpyCH4III ACNGT 4 cut(s) 145, 322, 958, 1034
HpyCH4IV ACGT 3 cut(s) 7, 53, 237
HpyCH4V TGCA 5 cut(s) 435, 464, 656, 919, 931
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 423, 432, 518, 773, 1004, 1024, 1091
HpyF3I CTNAG 2 cut(s) 259, 885
HpySE526I ACGT 3 cut(s) 7, 53, 237
Hsp92II CATG 6 cut(s) 212, 299, 430, 439, 740, 789
KflI GGGWCCC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 5 cut(s) 346, 457, 739, 749, 877
LmnI GCTCC 1 cut(s) 845
Lsp1109I GCAGC 3 cut(s) 70, 813, 991
LweI GCATC 3 cut(s) 106, 260, 940
MaeII ACGT 3 cut(s) 7, 53, 237
MaeIII GTNAC 5 cut(s) 658, 703, 754, 952, 958
MalI GATC 5 cut(s) 348, 459, 741, 751, 879
MboI GATC 5 cut(s) 346, 457, 739, 749, 877
MboII GAAGA 1 cut(s) 710
MfeI CAATTG 1 cut(s) 200
MhlI GDGCHC 1 cut(s) 842
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 5 cut(s) 200, 301, 402, 484, 611
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 1 cut(s) 875
MmeI TCCRAC 3 cut(s) 157, 544, 928
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
MseI TTAA 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 1 cut(s) 853
MspR9I CCNGG 2 cut(s) 393, 677
MunI CAATTG 1 cut(s) 200
MvaI CCWGG 2 cut(s) 393, 677
MvnI CGCG 2 cut(s) 38, 1023
MwoI GCNNNNNNNGC 8 cut(s) 116, 423, 432, 518, 773, 1004, 1024, 1091
NdeII GATC 5 cut(s) 346, 457, 739, 749, 877
NlaIII CATG 6 cut(s) 212, 299, 430, 439, 740, 789
NlaIV GGNNCC 3 cut(s) 503, 504, 893
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 2 cut(s) 658, 703
NruI TCGCGA 1 cut(s) 1023
NspI RCATGY 2 cut(s) 212, 789
PaeI GCATGC 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 225
PagI TCATGA 1 cut(s) 295
PciI ACATGT 1 cut(s) 785
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PfeI GAWTC 1 cut(s) 1043
PflMI CCANNNNNTGG 2 cut(s) 66, 682
PinAI ACCGGT 1 cut(s) 852
PkrI GCNGC 3 cut(s) 85, 803, 1006
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
PpuMI RGGWCCY 1 cut(s) 502
PscI ACATGT 1 cut(s) 785
Psp124BI GAGCTC 1 cut(s) 842
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 391, 675
PspGI CCWGG 2 cut(s) 391, 675
PspN4I GGNNCC 3 cut(s) 503, 504, 893
PspPI GGNCC 1 cut(s) 502
PspPPI RGGWCCY 1 cut(s) 502
PspXI VCTCGAGB 1 cut(s) 225
PstI CTGCAG 1 cut(s) 658
RruI TCGCGA 1 cut(s) 1023
RsaI GTAC 2 cut(s) 518, 812
RsaNI GTAC 2 cut(s) 517, 811
SacI GAGCTC 1 cut(s) 842
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 3 cut(s) 84, 802, 1005
Sau3AI GATC 5 cut(s) 346, 457, 739, 749, 877
Sau96I GGNCC 1 cut(s) 502
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 2 cut(s) 393, 677
SduI GDGCHC 1 cut(s) 842
SfaNI GCATC 3 cut(s) 106, 260, 940
SfcI CTRYAG 1 cut(s) 654
Sfr274I CTCGAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 502
SlaI CTCGAG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
SphI GCATGC 1 cut(s) 212
Sse9I AATT 5 cut(s) 200, 301, 402, 484, 611
SsiI CCGC 3 cut(s) 38, 291, 819
SstI GAGCTC 1 cut(s) 842
StyD4I CCNGG 2 cut(s) 391, 675
StyI CCWWGG 3 cut(s) 60, 605, 843
TaaI ACNGT 4 cut(s) 145, 322, 958, 1034
TaiI ACGT 3 cut(s) 10, 56, 240
TaqI TCGA 5 cut(s) 226, 232, 836, 934, 1096
TaqII GACCGA 1 cut(s) 137
TasI AATT 5 cut(s) 200, 301, 402, 484, 611
TatI WGTACW 1 cut(s) 810
TfiI GAWTC 1 cut(s) 1043
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 5 cut(s) 130, 136, 327, 344, 1039
TseFI GTSAC 2 cut(s) 658, 703
TseI GCWGC 3 cut(s) 83, 801, 1004
Tsp45I GTSAC 2 cut(s) 658, 703
TspDTI ATGAA 3 cut(s) 282, 312, 482
TspGWI ACGGA 1 cut(s) 922
TspRI CASTG 5 cut(s) 130, 136, 327, 344, 1039
Van91I CCANNNNNTGG 2 cut(s) 66, 682
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 1 cut(s) 301
XceI RCATGY 2 cut(s) 212, 789
XhoI CTCGAG 1 cut(s) 225
XmiI GTMKAC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.