pycom01g14530

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001059
Physical Location & Seq
Forward (+)
14666871 .. 14668231
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14530.2

Sequence Viewer

Length: 780 bp
ATGGCAGGTTTGTTTGACAAGCAAGCAGATCTGTACTTAGATTCAAGGCCAACTTATCCCAAAGAATGGTACTCCAAATTGGCAGATCTCACTCCTCACCACTCTCTCGCTTGGGATGTCGGAACCGGCAACGGCCAAGCCGCTATCGGTGTTGCTGAGCACTATAAACAAGTGATTGGAACCGATGTGAGTGAATCCCAGCTGCAGCGTGCAATGCCACATCCTCGTGTTCGATATGCCCACACGCCATTTACCATGACAAACGACGAATTAATAGCCATGATCGGGGGCGAAAACTCAGTTGACTTGGTGACTGTGGCTCAAGCTGTGCACTGGTTCGACTTACCAAAGTTTTACAGTCTTGTTTCGCGGCTTCTGAAGCCAGGAGGCGTGTTGGCGGTTTGGTGCTACAATCGCATCGAAGTTTGTCCGGAATTTGATCCTATTGTGAAGCGACTTGAGGACACAACCCTTCCCTATTATCATCGTAACATCCAGTACGTGTTTGATGGTTACAAGACGCTTCCTTTTCCTTTTCAGAGCGTTGGTTTCGGATGCGAGGGGAGTCCATTGGCGCTGGACATTCCAAAGAAGTTGTCGTTCGAAGGGTTTTTGAAGATGGTACGGTCATGGTCTGCAGTCAATACGGCCAAAGAACAAGGCGTGGATTTGTTGCCCGGAAAGCTGGTTAAAGAGTTTGAGGTTGCTTGGGGCGGCTCCAAATTAGTCAGGTCTGTCAGCTACAAGGCTTTTATGCTCGCCGGGAAAGTTAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

260

Amino Acids

29.04

Weight (kDa)

7.71

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 66
AccII CGCG 1 cut(s) 370
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 4 cut(s) 141, 370, 398, 714
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 2 cut(s) 133, 648
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 1 cut(s) 398
AfaI GTAC 4 cut(s) 35, 71, 500, 624
AfiI CCNNNNNNNGG 2 cut(s) 66, 285
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 2 cut(s) 45, 616
AjnI CCWGG 1 cut(s) 382
AluBI AGCT 4 cut(s) 202, 326, 685, 741
AluI AGCT 4 cut(s) 202, 326, 685, 741
Alw21I GWGCWC 2 cut(s) 162, 333
Alw44I GTGCAC 1 cut(s) 329
AlwI GGATC 1 cut(s) 434
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 3 cut(s) 47, 133, 648
ApaLI GTGCAC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 202, 205
ApoI RAATTY 1 cut(s) 434
ArsI GACNNNNNNTTYG 2 cut(s) 581, 613
AseI ATTAAT 1 cut(s) 272
AspLEI GCGC 1 cut(s) 577
AsuC2I CCSGG 2 cut(s) 678, 763
AsuHPI GGTGA 2 cut(s) 89, 322
AsuII TTCGAA 1 cut(s) 603
BaeGI GKGCMC 1 cut(s) 333
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 162, 333
BbvI GCAGC 2 cut(s) 189, 217
BccI CCATC 2 cut(s) 503, 613
BceAI ACGGC 2 cut(s) 148, 663
BciT130I CCWGG 1 cut(s) 384
BcnI CCSGG 2 cut(s) 678, 763
BfmI CTRYAG 2 cut(s) 203, 636
BfoI RGCGCY 1 cut(s) 578
BglII AGATCT 2 cut(s) 28, 85
BisI GCNGC 5 cut(s) 141, 203, 206, 371, 715
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 5 cut(s) 142, 204, 207, 372, 716
Bme1390I CCNGG 3 cut(s) 384, 678, 763
BmiI GGNNCC 3 cut(s) 124, 181, 718
BmrFI CCNGG 3 cut(s) 384, 678, 763
BmsI GCATC 2 cut(s) 426, 545
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 306, 479
BpuMI CCSGG 2 cut(s) 678, 763
BsaAI YACGTR 1 cut(s) 502
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 285
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 338, 496
Bse3DI GCAATG 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 384
BseGI GGATG 4 cut(s) 121, 220, 492, 560
BseLI CCNNNNNNNGG 2 cut(s) 66, 285
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 147, 312
BseNI ACTGG 2 cut(s) 338, 496
BseRI GAGGAG 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 2 cut(s) 189, 217
BseYI CCCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 49, 135, 650
BsiHKAI GWGCWC 2 cut(s) 162, 333
BsiSI CCGG 4 cut(s) 126, 431, 678, 762
BslI CCNNNNNNNGG 2 cut(s) 66, 285
BsnI GGCC 3 cut(s) 49, 135, 650
Bsp119I TTCGAA 1 cut(s) 603
Bsp1286I GDGCHC 2 cut(s) 162, 333
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 28, 85, 282, 439
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 4 cut(s) 141, 370, 398, 714
BspANI GGCC 3 cut(s) 49, 135, 650
BspCNI CTCAG 2 cut(s) 148, 311
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 124, 181, 718
BspMAI CTGCAG 2 cut(s) 207, 640
BspPI GGATC 1 cut(s) 434
BspT104I TTCGAA 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 338, 496
BssAI RCCGGY 1 cut(s) 125
BssMI GATC 4 cut(s) 28, 85, 282, 439
BssSI CACGAG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 3 cut(s) 316, 359, 627
BstBAI YACGTR 1 cut(s) 502
BstBI TTCGAA 1 cut(s) 603
BstC8I GCNNGC 3 cut(s) 24, 210, 759
BstDEI CTNAG 3 cut(s) 37, 156, 298
BstF5I GGATG 4 cut(s) 121, 220, 492, 560
BstFNI CGCG 1 cut(s) 370
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 4 cut(s) 31, 88, 285, 442
BstMBI GATC 4 cut(s) 28, 85, 282, 439
BstMWI GCNNNNNNNGC 4 cut(s) 214, 379, 414, 682
BstNI CCWGG 1 cut(s) 384
BstSCI CCNGG 3 cut(s) 382, 676, 761
BstSFI CTRYAG 2 cut(s) 203, 636
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 189, 217
BstX2I RGATCY 2 cut(s) 28, 85
BstYI RGATCY 2 cut(s) 28, 85
BsuRI GGCC 3 cut(s) 49, 135, 650
BtsCI GGATG 4 cut(s) 121, 220, 492, 560
BtsIMutI CAGTG 1 cut(s) 331
Cac8I GCNNGC 3 cut(s) 24, 210, 759
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 1 cut(s) 125
CseI GACGC 1 cut(s) 529
Csp6I GTAC 4 cut(s) 34, 70, 499, 623
CviAII CATG 3 cut(s) 256, 280, 630
CviQI GTAC 4 cut(s) 34, 70, 499, 623
DdeI CTNAG 3 cut(s) 37, 156, 298
DpnI GATC 4 cut(s) 30, 87, 284, 441
DpnII GATC 4 cut(s) 28, 85, 282, 439
EaeI YGGCCR 2 cut(s) 133, 648
Eco57I CTGAAG 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 382
FaeI CATG 3 cut(s) 259, 283, 633
FaiI YATR 6 cut(s) 165, 237, 257, 281, 631, 755
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FatI CATG 3 cut(s) 255, 279, 629
Fnu4HI GCNGC 5 cut(s) 141, 203, 206, 371, 715
FokI GGATG 4 cut(s) 128, 207, 479, 567
Fsp4HI GCNGC 5 cut(s) 141, 203, 206, 371, 715
GlaI GCGC 1 cut(s) 576
GluI GCNGC 5 cut(s) 141, 203, 206, 371, 715
GsaI CCCAGC 1 cut(s) 202
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 3 cut(s) 49, 135, 650
HapII CCGG 4 cut(s) 126, 431, 678, 762
HgaI GACGC 1 cut(s) 529
HhaI GCGC 1 cut(s) 577
Hin1II CATG 3 cut(s) 259, 283, 633
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 3 cut(s) 41, 194, 565
HpaII CCGG 4 cut(s) 126, 431, 678, 762
HphI GGTGA 2 cut(s) 89, 322
Hpy166II GTNNAC 2 cut(s) 304, 331
Hpy188I TCNGA 4 cut(s) 122, 378, 540, 554
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 2 cut(s) 304, 331
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 482, 599
HpyCH4III ACNGT 3 cut(s) 316, 359, 627
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 4 cut(s) 205, 212, 331, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 214, 379, 414, 682
HpyF3I CTNAG 3 cut(s) 37, 156, 298
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 3 cut(s) 259, 283, 633
HspAI GCGC 1 cut(s) 575
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 4 cut(s) 28, 85, 282, 439
LmnI GCTCC 1 cut(s) 722
Lsp1109I GCAGC 2 cut(s) 189, 217
LweI GCATC 2 cut(s) 426, 545
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 3 cut(s) 310, 488, 512
MalI GATC 4 cut(s) 30, 87, 284, 441
MboI GATC 4 cut(s) 28, 85, 282, 439
MboII GAAGA 1 cut(s) 628
MflI RGATCY 2 cut(s) 28, 85
MhlI GDGCHC 2 cut(s) 162, 333
MluCI AATT 4 cut(s) 77, 269, 434, 722
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 6 cut(s) 105, 234, 380, 454, 553, 694
MroI TCCGGA 1 cut(s) 430
MseI TTAA 3 cut(s) 272, 690, 771
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 4 cut(s) 126, 431, 678, 762
MspR9I CCNGG 3 cut(s) 384, 678, 763
MvaI CCWGG 1 cut(s) 384
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 4 cut(s) 214, 379, 414, 682
NciI CCSGG 2 cut(s) 678, 763
NdeII GATC 4 cut(s) 28, 85, 282, 439
NlaIII CATG 3 cut(s) 259, 283, 633
NlaIV GGNNCC 3 cut(s) 124, 181, 718
NmuCI GTSAC 1 cut(s) 310
NspV TTCGAA 1 cut(s) 603
PfeI GAWTC 2 cut(s) 41, 194
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 5 cut(s) 142, 204, 207, 372, 716
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
Ppu21I YACGTR 1 cut(s) 502
PshBI ATTAAT 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 3 cut(s) 124, 181, 718
PstI CTGCAG 2 cut(s) 207, 640
PsuI RGATCY 2 cut(s) 28, 85
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 4 cut(s) 35, 71, 500, 624
RsaNI GTAC 4 cut(s) 34, 70, 499, 623
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 3 cut(s) 272, 690, 771
SatI GCNGC 5 cut(s) 141, 203, 206, 371, 715
Sau3AI GATC 4 cut(s) 28, 85, 282, 439
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 3 cut(s) 384, 678, 763
SduI GDGCHC 2 cut(s) 162, 333
SetI ASST 9 cut(s) 10, 204, 328, 504, 687, 705, 734, 743, 777
SfaNI GCATC 2 cut(s) 426, 545
SfcI CTRYAG 2 cut(s) 203, 636
SfuI TTCGAA 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 321, 458
SmoI CTYRAG 2 cut(s) 321, 458
Sse9I AATT 4 cut(s) 77, 269, 434, 722
SsiI CCGC 4 cut(s) 141, 370, 398, 714
StyD4I CCNGG 3 cut(s) 382, 676, 761
TaaI ACNGT 3 cut(s) 316, 359, 627
TaiI ACGT 1 cut(s) 504
TaqI TCGA 4 cut(s) 232, 339, 420, 603
TasI AATT 4 cut(s) 77, 269, 434, 722
TatI WGTACW 1 cut(s) 33
TauI GCSGC 3 cut(s) 143, 373, 717
TfiI GAWTC 2 cut(s) 41, 194
Tru1I TTAA 3 cut(s) 272, 690, 771
Tru9I TTAA 3 cut(s) 272, 690, 771
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 202, 205
Tsp45I GTSAC 1 cut(s) 310
TspRI CASTG 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 66
VneI GTGCAC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 272
XapI RAATTY 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.