Rw7G014880

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001059
Physical Location & Seq
Reverse (-)
14674469 .. 14676252
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014880.1

Sequence Viewer

Length: 783 bp
ATGGCAGGTCTATTCGACAAGCAAGCAGAGGTGTACTTGGAAGCTCGGCCAACATATCCCAAGGAATGGTATTTGAAGCTGGCCGGTCTCACCCCCGAACACTCTTTAGCTTGGGATGTTGGAACTGGAAATGGCCAAGCTGCTTATAGTCTTTCAGAGCATTACGAGCAAGTAGTGGGAAGCGACATCAGTGGAGAACAATTAAAAAATGCCATCCAACATCCTCGAATTCGATACATTCATACTCCAACATCAATCTCAGAGGATGAAATGGTCAACTTAATAGCCGGTGGTCACGAAAATTCAGTGGACTTGGTTACAGCGGCGGAAGCTGTTCACTGGTTTGATCTTCCTCATTTCTATTCACTCGTCAGACGCCTTCTGCGCAAACCAGGAGGCGTAATTGCAGTTTGGGGCTACAACGACATGGTAGTAAGCCCCGATTTTGATCTTTTAATGAAGCGTTACCACGAAAAGATGTTACCATATTATGACCCGAAATCAAGCTACTTATGGGAGGGTTATAGGACACTTCCATTTCCATTTGAGAGTGTTGGATTAGGTCGTGAAGGAGAGCCTATGGCACTTGAAATACATAAGGAACTTTCATTTCAGGGGCTTCTGAAGTTTCTGAGGTCTTCCTCTGCAGTCACTACGGCTAAAGACAAGGGTGTGGATTTGTTGTCTGAAGAGGTGATTGAAGAGATTCAGGGAGTTTGGGGTGGGCCTGATCTGGTTAGATCTGTTACGTACAAGGCTTTTATGCTTGCTGGCAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

260

Amino Acids

29.28

Weight (kDa)

5.39

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 39 - 136 4.9e-10 Methyltransferase domain
Methyltransf_25 PF13649 39 - 132 1.1e-08 Methyltransferase domain
Methyltransf_12 PF08242 39 - 134 7.9e-07 Methyltransferase domain
Methyltransf_31 PF13847 39 - 135 3e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 66
AciI CCGC 2 cut(s) 323, 326
AcoI YGGCCR 3 cut(s) 47, 81, 133
AcsI RAATTY 2 cut(s) 228, 301
AcuI CTGAAG 2 cut(s) 644, 708
AcyI GRCGYC 1 cut(s) 376
AfaI GTAC 2 cut(s) 35, 752
AfiI CCNNNNNNNGG 1 cut(s) 66
AgsI TTSAA 3 cut(s) 76, 590, 701
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 6 cut(s) 44, 79, 110, 140, 332, 507
AluI AGCT 6 cut(s) 44, 79, 110, 140, 332, 507
Alw26I GTCTC 1 cut(s) 92
AoxI GGCC 4 cut(s) 47, 81, 133, 725
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 228, 301
Asp700I GAANNNNTTC 2 cut(s) 333, 705
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 725
AsuHPI GGTGA 2 cut(s) 82, 706
BalI TGGCCA 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 127
BccI CCATC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 92
BfmI CTRYAG 1 cut(s) 645
BglII AGATCT 1 cut(s) 740
BisI GCNGC 2 cut(s) 141, 324
BlsI GCNGC 2 cut(s) 142, 325
Bme1390I CCNGG 1 cut(s) 393
BmgT120I GGNCC 1 cut(s) 725
BmrFI CCNGG 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 630
BsaAI YACGTR 1 cut(s) 750
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse118I RCCGGY 2 cut(s) 83, 287
Bse1I ACTGG 2 cut(s) 130, 344
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 4 cut(s) 121, 213, 220, 271
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMII CTCAG 2 cut(s) 273, 623
BseNI ACTGG 2 cut(s) 130, 344
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 4 cut(s) 49, 83, 135, 727
BsiSI CCGG 2 cut(s) 84, 288
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 4 cut(s) 49, 83, 135, 727
Bso31I GGTCTC 1 cut(s) 92
Bsp143I GATC 4 cut(s) 346, 448, 730, 740
BspACI CCGC 2 cut(s) 323, 326
BspANI GGCC 4 cut(s) 49, 83, 135, 727
BspCNI CTCAG 2 cut(s) 272, 624
BspMAI CTGCAG 1 cut(s) 649
BspTNI GGTCTC 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 83, 287
BsrI ACTGG 2 cut(s) 130, 344
BssAI RCCGGY 2 cut(s) 83, 287
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 4 cut(s) 346, 448, 730, 740
BssNI GRCGYC 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 393
Bst6I CTCTTC 2 cut(s) 684, 696
BstACI GRCGYC 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 750
BstC8I GCNNGC 4 cut(s) 24, 81, 768, 772
BstDEI CTNAG 2 cut(s) 259, 632
BstF5I GGATG 4 cut(s) 121, 213, 220, 271
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 4 cut(s) 349, 451, 733, 743
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 4 cut(s) 346, 448, 730, 740
BstMWI GCNNNNNNNGC 3 cut(s) 166, 329, 384
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 645
BstSNI TACGTA 1 cut(s) 750
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 740
BstYI RGATCY 1 cut(s) 740
BsuRI GGCC 4 cut(s) 49, 83, 135, 727
BtsCI GGATG 4 cut(s) 121, 213, 220, 271
BtsIMutI CAGTG 3 cut(s) 196, 312, 337
Cac8I GCNNGC 4 cut(s) 24, 81, 768, 772
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 2 cut(s) 83, 287
Cfr13I GGNCC 1 cut(s) 725
CseI GACGC 1 cut(s) 384
Csp6I GTAC 2 cut(s) 34, 751
CviAII CATG 1 cut(s) 427
CviQI GTAC 2 cut(s) 34, 751
DdeI CTNAG 2 cut(s) 259, 632
DpnI GATC 4 cut(s) 348, 450, 732, 742
DpnII GATC 4 cut(s) 346, 448, 730, 740
EaeI YGGCCR 3 cut(s) 47, 81, 133
Eam1104I CTCTTC 2 cut(s) 684, 696
EarI CTCTTC 2 cut(s) 684, 696
EciI GGCGGA 1 cut(s) 341
Eco105I TACGTA 1 cut(s) 750
Eco130I CCWWGG 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 92
Eco57I CTGAAG 2 cut(s) 644, 708
EcoRI GAATTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 1 cut(s) 430
FatI CATG 1 cut(s) 426
Fnu4HI GCNGC 2 cut(s) 141, 324
FokI GGATG 4 cut(s) 128, 200, 207, 278
Fsp4HI GCNGC 2 cut(s) 141, 324
FspI TGCGCA 1 cut(s) 386
GlaI GCGC 1 cut(s) 386
GluI GCNGC 2 cut(s) 141, 324
HaeIII GGCC 4 cut(s) 49, 83, 135, 727
HapII CCGG 2 cut(s) 84, 288
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 387
Hin1I GRCGYC 1 cut(s) 376
Hin1II CATG 1 cut(s) 430
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 1 cut(s) 706
HpaII CCGG 2 cut(s) 84, 288
HphI GGTGA 2 cut(s) 82, 706
Hpy166II GTNNAC 4 cut(s) 34, 277, 310, 337
Hpy188I TCNGA 6 cut(s) 157, 262, 374, 624, 633, 688
Hpy188III TCNNGA 2 cut(s) 296, 566
Hpy8I GTNNAC 4 cut(s) 34, 277, 310, 337
HpyAV CCTTC 2 cut(s) 389, 563
HpyCH4IV ACGT 1 cut(s) 749
HpyCH4V TGCA 2 cut(s) 407, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 329, 384
HpyF3I CTNAG 2 cut(s) 259, 632
HpySE526I ACGT 1 cut(s) 749
Hsp92I GRCGYC 1 cut(s) 376
Hsp92II CATG 1 cut(s) 430
HspAI GCGC 1 cut(s) 385
Kzo9I GATC 4 cut(s) 346, 448, 730, 740
Lsp1109I GCAGC 1 cut(s) 127
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 6 cut(s) 293, 316, 464, 480, 649, 745
MalI GATC 4 cut(s) 348, 450, 732, 742
MboI GATC 4 cut(s) 346, 448, 730, 740
MboII GAAGA 4 cut(s) 341, 630, 701, 713
MflI RGATCY 1 cut(s) 740
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 4 cut(s) 200, 228, 301, 402
MluNI TGGCCA 1 cut(s) 135
MmeI TCCRAC 4 cut(s) 100, 241, 272, 535
MnlI CCTC 9 cut(s) 22, 234, 256, 363, 389, 511, 627, 652, 685
Mox20I TGGCCA 1 cut(s) 135
MroXI GAANNNNTTC 2 cut(s) 333, 705
MscI TGGCCA 1 cut(s) 135
MseI TTAA 3 cut(s) 203, 281, 455
Msp20I TGGCCA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 2 cut(s) 84, 288
MspR9I CCNGG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 3 cut(s) 166, 329, 384
NdeII GATC 4 cut(s) 346, 448, 730, 740
NlaIII CATG 1 cut(s) 430
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 2 cut(s) 293, 649
NsbI TGCGCA 1 cut(s) 386
PdmI GAANNNNTTC 2 cut(s) 333, 705
PfeI GAWTC 1 cut(s) 706
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 2 cut(s) 142, 325
Ppu21I YACGTR 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspPI GGNCC 1 cut(s) 725
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 740
RsaI GTAC 2 cut(s) 35, 752
RsaNI GTAC 2 cut(s) 34, 751
SaqAI TTAA 3 cut(s) 203, 281, 455
SatI GCNGC 2 cut(s) 141, 324
Sau3AI GATC 4 cut(s) 346, 448, 730, 740
Sau96I GGNCC 1 cut(s) 725
ScrFI CCNGG 1 cut(s) 393
SfcI CTRYAG 1 cut(s) 645
SnaBI TACGTA 1 cut(s) 750
Sse9I AATT 4 cut(s) 200, 228, 301, 402
SsiI CCGC 2 cut(s) 323, 326
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 60
TaiI ACGT 1 cut(s) 752
TaqI TCGA 3 cut(s) 15, 226, 232
TasI AATT 4 cut(s) 200, 228, 301, 402
TatI WGTACW 1 cut(s) 33
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 706
Tru1I TTAA 3 cut(s) 203, 281, 455
Tru9I TTAA 3 cut(s) 203, 281, 455
TscAI CASTG 3 cut(s) 196, 312, 344
TseFI GTSAC 2 cut(s) 293, 649
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 293, 649
TspDTI ATGAA 4 cut(s) 230, 282, 473, 597
TspRI CASTG 3 cut(s) 196, 312, 344
Van91I CCANNNNNTGG 1 cut(s) 66
XapI RAATTY 2 cut(s) 228, 301
XmnI GAANNNNTTC 2 cut(s) 333, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.