Prupe.1G580000_v2.0.a1

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001059
Physical Location & Seq
Reverse (-)
47178448 .. 47179513
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G580000.1

Sequence Viewer

Length: 780 bp
ATGTGGGTAACCTACCTCTATGTTTGTACTTCTCTTTCGATTGAAATGAAAAGAGATACAAGGCAAAAAATTGCAGGTTTATTCGACAAGCAGGTCGATGATTACTTGGTGGGAAGGCCAACTTATCCCAAGGAATGGTTTTCAATGTTGGCAGCTCTTACCCCCCAACACTCTTTAGCTTGGGACCTTGGAGAGCATTATGAGCAAGTGATAGGAAGCGATATAAGTGAGTCCCAACTGAAGCATGCACTTCAACATCCTCGAGTTCATTACATTCACACTCCGGTCTCCATGTCTGATGACGAATTGGTTGCGTTATTAGGGGGTGAGAATTCAGTTGATTTGGTAACCGTGGCAGAGGCCGTGCACTGGTTTGACCTCCCAAAGTTCTACTCACTCGTGAAGCGAGTTCTGCGTAAACCAGGAGGCGTAATCGCTGTTTGGGGCTACAATGCTAGTGTGACAAGCCCTACACTTGATGGTGTAGTGAAGCGTTTTTATGAAACAGCTAAACCCTTTCGGGACCGAAAGACGCAGTACTTATGGGAAGGTTATAGGACACTTCCATTTCCATTTCAAAGTGTTGGTTTGGGTTCTGAGGGTGAGCCTCTGCCACTAGAAATACCAAAGGAAATGTCATTTGAAGGGATCTTAAGGATGTTAAGATCCTCCTCTGCAGTCACTGCCGCTAAGGGTCAAGGTGTGGATTTGTTATCAAAAGAGGTTATAGAAGAGCTTGAAACTACTTGCAAGGCTTTTATGCTTGCTGGAAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

260

Amino Acids

29.2

Weight (kDa)

6.0

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
Acc36I ACCTGC 2 cut(s) 65, 82
AccB7I CCANNNNNTGG 1 cut(s) 135
AciI CCGC 1 cut(s) 687
AclWI GGATC 2 cut(s) 656, 660
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 2 cut(s) 28, 539
AfiI CCNNNNNNNGG 2 cut(s) 135, 369
AflII CTTAAG 1 cut(s) 652
AgsI TTSAA 6 cut(s) 44, 144, 254, 578, 644, 740
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 4 cut(s) 155, 179, 509, 736
AluI AGCT 4 cut(s) 155, 179, 509, 736
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 292
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 2 cut(s) 656, 660
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 2 cut(s) 116, 360
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 331
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AspS9I GGNCC 2 cut(s) 184, 523
AsuHPI GGTGA 2 cut(s) 338, 614
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 184, 523
BaeGI GKGCMC 1 cut(s) 369
BauI CACGAG 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 347
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 292
BfaI CTAG 2 cut(s) 456, 617
BfmI CTRYAG 1 cut(s) 675
BfrI CTTAAG 1 cut(s) 652
BfuAI ACCTGC 2 cut(s) 65, 82
BisI GCNGC 2 cut(s) 153, 687
BlsI GCNGC 2 cut(s) 154, 688
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 2 cut(s) 184, 523
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 184, 523
BmiI GGNNCC 2 cut(s) 185, 524
BmrFI CCNGG 1 cut(s) 423
Bpu10I CCTNAGC 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 129, 187, 351
BsaWI WCCGGW 1 cut(s) 283
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 369
Bse1I ACTGG 1 cut(s) 374
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 3 cut(s) 129, 187, 351
BseGI GGATG 2 cut(s) 256, 663
BseLI CCNNNNNNNGG 2 cut(s) 135, 369
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 374
BseRI GAGGAG 1 cut(s) 661
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 1 cut(s) 164
BshFI GGCC 2 cut(s) 118, 362
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 3 cut(s) 197, 217, 536
BslI CCNNNNNNNGG 2 cut(s) 135, 369
BsmAI GTCTC 1 cut(s) 292
BsmFI GGGAC 3 cut(s) 197, 217, 536
BsnI GGCC 2 cut(s) 118, 362
Bso31I GGTCTC 1 cut(s) 292
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 2 cut(s) 648, 665
BspACI CCGC 1 cut(s) 687
BspANI GGCC 2 cut(s) 118, 362
BspCNI CTCAG 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 185, 524
BspMAI CTGCAG 1 cut(s) 679
BspMI ACCTGC 2 cut(s) 65, 82
BspPI GGATC 2 cut(s) 656, 660
BspQI GCTCTTC 1 cut(s) 726
BspTI CTTAAG 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 292
BsrI ACTGG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 129, 187, 351
BssMI GATC 2 cut(s) 648, 665
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 2 cut(s) 129, 187
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 726
BstAFI CTTAAG 1 cut(s) 652
BstAPI GCANNNNNTGC 1 cut(s) 683
BstC8I GCNNGC 2 cut(s) 246, 765
BstDEI CTNAG 2 cut(s) 597, 690
BstDSI CCRYGG 1 cut(s) 351
BstEII GGTNACC 2 cut(s) 7, 346
BstF5I GGATG 2 cut(s) 256, 663
BstKTI GATC 2 cut(s) 651, 668
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 2 cut(s) 648, 665
BstMWI GCNNNNNNNGC 3 cut(s) 202, 412, 683
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 248
BstPI GGTNACC 2 cut(s) 7, 346
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 675
BstSLI GKGCMC 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 2 cut(s) 648, 665
BstYI RGATCY 2 cut(s) 648, 665
BsuRI GGCC 2 cut(s) 118, 362
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 256, 663
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 2 cut(s) 367, 681
BveI ACCTGC 2 cut(s) 65, 82
Cac8I GCNNGC 2 cut(s) 246, 765
CaiI CAGNNNCTG 1 cut(s) 683
Cfr13I GGNCC 2 cut(s) 184, 523
CseI GACGC 1 cut(s) 541
Csp6I GTAC 2 cut(s) 27, 538
CviAII CATG 2 cut(s) 245, 292
CviQI GTAC 2 cut(s) 27, 538
DdeI CTNAG 2 cut(s) 597, 690
DpnI GATC 2 cut(s) 650, 667
DpnII GATC 2 cut(s) 648, 665
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Eco130I CCWWGG 2 cut(s) 129, 187
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 184, 523
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 2 cut(s) 7, 346
EcoO109I RGGNCCY 1 cut(s) 184
EcoO65I GGTNACC 2 cut(s) 7, 346
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 421
EcoT14I CCWWGG 2 cut(s) 129, 187
ErhI CCWWGG 2 cut(s) 129, 187
FaeI CATG 2 cut(s) 248, 295
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 3 cut(s) 197, 217, 536
FatI CATG 2 cut(s) 244, 291
Fnu4HI GCNGC 2 cut(s) 153, 687
FokI GGATG 2 cut(s) 243, 670
Fsp4HI GCNGC 2 cut(s) 153, 687
FspBI CTAG 2 cut(s) 456, 617
GluI GCNGC 2 cut(s) 153, 687
HaeIII GGCC 2 cut(s) 118, 362
HapII CCGG 1 cut(s) 284
HgaI GACGC 1 cut(s) 541
Hin1II CATG 2 cut(s) 248, 295
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 284
HphI GGTGA 2 cut(s) 338, 614
Hpy166II GTNNAC 2 cut(s) 367, 419
Hpy188I TCNGA 2 cut(s) 298, 598
Hpy188III TCNNGA 2 cut(s) 400, 521
Hpy8I GTNNAC 2 cut(s) 367, 419
HpyAV CCTTC 3 cut(s) 108, 542, 638
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 5 cut(s) 74, 248, 367, 677, 750
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 412, 683
HpyF3I CTNAG 2 cut(s) 597, 690
Hsp92II CATG 2 cut(s) 248, 295
Kzo9I GATC 2 cut(s) 648, 665
LguI GCTCTTC 1 cut(s) 726
LpnPI CCDG 7 cut(s) 60, 77, 297, 355, 408, 435, 753
Lsp1109I GCAGC 1 cut(s) 164
MaeI CTAG 2 cut(s) 456, 617
MaeIII GTNAC 4 cut(s) 7, 346, 460, 679
MalI GATC 2 cut(s) 650, 667
MboI GATC 2 cut(s) 648, 665
MboII GAAGA 1 cut(s) 743
MflI RGATCY 2 cut(s) 648, 665
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 3 cut(s) 69, 305, 331
MlyI GAGTC 1 cut(s) 239
MseI TTAA 3 cut(s) 653, 662, 778
MspCI CTTAAG 1 cut(s) 652
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 423
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 3 cut(s) 202, 412, 683
NdeII GATC 2 cut(s) 648, 665
NlaIII CATG 2 cut(s) 248, 295
NlaIV GGNNCC 2 cut(s) 185, 524
NmuCI GTSAC 2 cut(s) 460, 679
NspI RCATGY 1 cut(s) 248
PaeI GCATGC 1 cut(s) 248
PaeR7I CTCGAG 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 726
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 2 cut(s) 154, 688
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 421
PspEI GGTNACC 2 cut(s) 7, 346
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 185, 524
PspPI GGNCC 2 cut(s) 184, 523
PspPPI RGGWCCY 1 cut(s) 184
PspXI VCTCGAGB 1 cut(s) 261
PstI CTGCAG 1 cut(s) 679
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 2 cut(s) 648, 665
RsaI GTAC 2 cut(s) 28, 539
RsaNI GTAC 2 cut(s) 27, 538
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 3 cut(s) 653, 662, 778
SatI GCNGC 2 cut(s) 153, 687
Sau3AI GATC 2 cut(s) 648, 665
Sau96I GGNCC 2 cut(s) 184, 523
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 1 cut(s) 369
SfcI CTRYAG 1 cut(s) 675
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 184, 523
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 261, 652
SmoI CTYRAG 2 cut(s) 261, 652
SphI GCATGC 1 cut(s) 248
Sse9I AATT 3 cut(s) 69, 305, 331
SsiI CCGC 1 cut(s) 687
SspMI CTAG 2 cut(s) 456, 617
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 2 cut(s) 129, 187
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 4 cut(s) 38, 84, 96, 262
TaqII GACCGA 1 cut(s) 540
TasI AATT 3 cut(s) 69, 305, 331
TatI WGTACW 2 cut(s) 26, 537
TauI GCSGC 1 cut(s) 689
Tru1I TTAA 3 cut(s) 653, 662, 778
Tru9I TTAA 3 cut(s) 653, 662, 778
TscAI CASTG 2 cut(s) 374, 688
TseFI GTSAC 2 cut(s) 460, 679
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 460, 679
TspDTI ATGAA 3 cut(s) 62, 257, 516
TspRI CASTG 2 cut(s) 374, 688
Van91I CCANNNNNTGG 1 cut(s) 135
Vha464I CTTAAG 1 cut(s) 652
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 184, 523
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 261
XspI CTAG 2 cut(s) 456, 617
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.