pycom08g21040

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001059
Physical Location & Seq
Reverse (-)
20619997 .. 20628965
8969 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21040.3

Sequence Viewer

Length: 750 bp
ATGCTGGCCGCTCTCACACCAAATCACTCCGTAGCTTGGGATGTCGGCACTGGCAATGGCCAAGCTGCTTTCTCTCTTGGAGAGCATTATGAGCAAGTAATAGGAAGTGATATAAGTGAGTCCCAATTGAAGCTGGCACTCCAACATCCTCGAGTTCGTTACGTTCACACCCCGATGACCATGTCTGACGACGATTTGGTTGAGTTAATTGGGGGTGAAAACTCAGTTGATTTGGTGACCGTGGCAGAGGCTGTGCACTGGTTCGACCTCCCAAGGTTCTACTCGGTCGTCAAGCGCGTTTTGCGTAAGCCAGGAGGCATAATTGCAGTTTGGGGCTACAATGGTAGCGTAACAGACCCCGAACTCGACAATTTATTGACGCATTTTCATGAAGCAAGTAAACCGTTTAGGGACCCGAAGACGGAGTACTTGTGGGAAGGGTACAGGACACTTCCATTTCCATTTGAAAGTGTTGGTTTGGGTTCTGCAGGTCAGCCTGTGCCACTGGAAATGCCAAAGGAGATGTCATTTGAAGGGGTGATGAGGATGATCAGGTCCTCGACTGCATTCATTACAGCTAAAGATCAAGGTGTGGATTTGTTATCACAAGAGGTGATTGAAGAGCTTCAAAGAGCTTGGGGTGGCCCTAACTTGGTCAGAAAAATTACTTCCAAGGCATTTATGCTTGCCGGAAAAACTGGTTCAGCGTATCCCTTCCAAAAGCAAAAGCTGTACTCCGTTCTTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

250

Amino Acids

27.7

Weight (kDa)

5.82

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
AccBSI CCGCTC 1 cut(s) 11
AccII CGCG 1 cut(s) 297
AciI CCGC 1 cut(s) 9
AcoI YGGCCR 2 cut(s) 6, 58
AfaI GTAC 3 cut(s) 428, 443, 734
AfiI CCNNNNNNNGG 3 cut(s) 36, 421, 652
AgsI TTSAA 5 cut(s) 130, 467, 533, 620, 629
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 7 cut(s) 35, 65, 133, 578, 625, 635, 730
AluI AGCT 7 cut(s) 35, 65, 133, 578, 625, 635, 730
Alw21I GWGCWC 1 cut(s) 258
Alw44I GTGCAC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 251
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 3 cut(s) 6, 58, 643
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 65
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
Asp700I GAANNNNTTC 1 cut(s) 624
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 3 cut(s) 412, 555, 644
AsuHPI GGTGA 4 cut(s) 227, 247, 550, 625
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 2 cut(s) 412, 555
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 60
BarI GAAGNNNNNNTAC 2 cut(s) 410, 442
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 312
BciVI GTATCC 1 cut(s) 720
BclI TGATCA 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 486
BfuAI ACCTGC 1 cut(s) 479
BfuI GTATCC 1 cut(s) 720
BisI GCNGC 2 cut(s) 9, 66
BlsI GCNGC 2 cut(s) 10, 67
BmcAI AGTACT 1 cut(s) 428
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 2 cut(s) 412, 555
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 3 cut(s) 412, 555, 644
BmiI GGNNCC 2 cut(s) 413, 414
BmrFI CCNGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 425
BsaJI CCNNGG 3 cut(s) 240, 272, 672
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 421, 652
Bse1I ACTGG 4 cut(s) 55, 263, 510, 703
Bse3DI GCAATG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 3 cut(s) 240, 272, 672
BseGI GGATG 3 cut(s) 46, 145, 552
BseLI CCNNNNNNNGG 3 cut(s) 36, 421, 652
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 4 cut(s) 55, 263, 510, 703
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 3 cut(s) 8, 60, 645
BsiEI CGRYCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 2 cut(s) 106, 425
BslI CCNNNNNNNGG 3 cut(s) 36, 421, 652
BsmFI GGGAC 2 cut(s) 106, 425
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 3 cut(s) 8, 60, 645
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 2 cut(s) 549, 583
BspACI CCGC 1 cut(s) 9
BspANI GGCC 3 cut(s) 8, 60, 645
BspCNI CTCAG 1 cut(s) 236
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 413, 414
BspMAI CTGCAG 1 cut(s) 490
BspMI ACCTGC 1 cut(s) 479
BspQI GCTCTTC 1 cut(s) 615
BsrBI CCGCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 61
BsrI ACTGG 4 cut(s) 55, 263, 510, 703
BssECI CCNNGG 3 cut(s) 240, 272, 672
BssMI GATC 2 cut(s) 549, 583
BssT1I CCWWGG 2 cut(s) 272, 672
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 241, 405
Bst6I CTCTTC 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 6, 135, 687
BstDEI CTNAG 1 cut(s) 223
BstDSI CCRYGG 1 cut(s) 240
BstEII GGTNACC 1 cut(s) 235
BstF5I GGATG 3 cut(s) 46, 145, 552
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 2 cut(s) 552, 586
BstMBI GATC 2 cut(s) 549, 583
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 2 cut(s) 91, 301
BstNI CCWGG 1 cut(s) 312
BstPI GGTNACC 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 52
BstV2I GAAGAC 1 cut(s) 425
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 3 cut(s) 8, 60, 645
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 3 cut(s) 46, 145, 552
BtsIMutI CAGTG 3 cut(s) 48, 256, 503
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 3 cut(s) 6, 135, 687
CaiI CAGNNNCTG 1 cut(s) 251
CciI TCATGA 1 cut(s) 388
CfoI GCGC 1 cut(s) 297
Cfr13I GGNCC 3 cut(s) 412, 555, 644
CseI GACGC 1 cut(s) 388
Csp6I GTAC 3 cut(s) 427, 442, 733
CviAII CATG 2 cut(s) 181, 389
CviQI GTAC 3 cut(s) 427, 442, 733
DdeI CTNAG 1 cut(s) 223
DpnI GATC 2 cut(s) 551, 585
DpnII GATC 2 cut(s) 549, 583
EaeI YGGCCR 2 cut(s) 6, 58
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 272, 672
Eco47I GGWCC 2 cut(s) 412, 555
Eco88I CYCGRG 1 cut(s) 150
Eco91I GGTNACC 1 cut(s) 235
EcoO109I RGGNCCY 2 cut(s) 412, 555
EcoO65I GGTNACC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 310
EcoT14I CCWWGG 2 cut(s) 272, 672
ErhI CCWWGG 2 cut(s) 272, 672
FaeI CATG 2 cut(s) 184, 392
FaiI YATR 6 cut(s) 90, 113, 182, 320, 390, 683
FaqI GGGAC 2 cut(s) 106, 425
FatI CATG 2 cut(s) 180, 388
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 2 cut(s) 9, 66
FokI GGATG 3 cut(s) 53, 132, 559
Fsp4HI GCNGC 2 cut(s) 9, 66
GlaI GCGC 1 cut(s) 296
GluI GCNGC 2 cut(s) 9, 66
HaeIII GGCC 3 cut(s) 8, 60, 645
HapII CCGG 1 cut(s) 690
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 297
Hin1II CATG 2 cut(s) 184, 392
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 690
HphI GGTGA 4 cut(s) 227, 247, 550, 625
Hpy166II GTNNAC 3 cut(s) 166, 256, 401
Hpy188I TCNGA 2 cut(s) 187, 659
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 3 cut(s) 166, 256, 401
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 431, 527, 724
HpyCH4III ACNGT 2 cut(s) 241, 405
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 4 cut(s) 256, 326, 488, 566
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 301
HpyF3I CTNAG 1 cut(s) 223
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 2 cut(s) 184, 392
HspAI GCGC 1 cut(s) 295
KflI GGGWCCC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 2 cut(s) 549, 583
LguI GCTCTTC 1 cut(s) 615
Lsp1109I GCAGC 1 cut(s) 52
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 3 cut(s) 158, 235, 349
MalI GATC 2 cut(s) 551, 585
MbiI CCGCTC 1 cut(s) 11
MboI GATC 2 cut(s) 549, 583
MboII GAAGA 3 cut(s) 430, 632, 734
MfeI CAATTG 1 cut(s) 125
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 5 cut(s) 125, 207, 321, 370, 663
MluNI TGGCCA 1 cut(s) 60
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 7 cut(s) 159, 241, 278, 308, 537, 568, 604
Mox20I TGGCCA 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 624
MscI TGGCCA 1 cut(s) 60
MseI TTAA 1 cut(s) 206
MslI CAYNNNNRTG 2 cut(s) 173, 387
Msp20I TGGCCA 1 cut(s) 60
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 1 cut(s) 312
MunI CAATTG 1 cut(s) 125
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 91, 301
NdeII GATC 2 cut(s) 549, 583
NlaIII CATG 2 cut(s) 184, 392
NlaIV GGNNCC 2 cut(s) 413, 414
NmuCI GTSAC 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 150
PagI TCATGA 1 cut(s) 388
PciSI GCTCTTC 1 cut(s) 615
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 624
PflFI GACNNNGTC 1 cut(s) 181
PkrI GCNGC 2 cut(s) 10, 67
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PpuMI RGGWCCY 2 cut(s) 412, 555
Psp5II RGGWCCY 2 cut(s) 412, 555
Psp6I CCWGG 1 cut(s) 310
PspEI GGTNACC 1 cut(s) 235
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 2 cut(s) 413, 414
PspPI GGNCC 3 cut(s) 412, 555, 644
PspPPI RGGWCCY 2 cut(s) 412, 555
PspXI VCTCGAGB 1 cut(s) 150
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 251
PsyI GACNNNGTC 1 cut(s) 181
RsaI GTAC 3 cut(s) 428, 443, 734
RsaNI GTAC 3 cut(s) 427, 442, 733
RseI CAYNNNNRTG 2 cut(s) 173, 387
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 2 cut(s) 9, 66
Sau3AI GATC 2 cut(s) 549, 583
Sau96I GGNCC 3 cut(s) 412, 555, 644
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 1 cut(s) 258
SfcI CTRYAG 1 cut(s) 486
Sfr274I CTCGAG 1 cut(s) 150
SinI GGWCC 2 cut(s) 412, 555
SlaI CTCGAG 1 cut(s) 150
SmiMI CAYNNNNRTG 2 cut(s) 173, 387
SmlI CTYRAG 1 cut(s) 150
SmoI CTYRAG 1 cut(s) 150
Sse9I AATT 5 cut(s) 125, 207, 321, 370, 663
SsiI CCGC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 2 cut(s) 272, 672
TaaI ACNGT 2 cut(s) 241, 405
TaiI ACGT 1 cut(s) 165
TaqI TCGA 4 cut(s) 151, 264, 366, 560
TaqII GACCGA 1 cut(s) 274
TasI AATT 5 cut(s) 125, 207, 321, 370, 663
TatI WGTACW 2 cut(s) 426, 732
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TscAI CASTG 3 cut(s) 55, 263, 510
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 3 cut(s) 377, 405, 559
TspGWI ACGGA 3 cut(s) 19, 437, 727
TspRI CASTG 3 cut(s) 55, 263, 510
Tth111I GACNNNGTC 1 cut(s) 181
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 412, 555
XhoI CTCGAG 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 624
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.