MD07G1186100.v1.1

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001059
Physical Location & Seq
Forward (+)
26714461 .. 26716057
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1186100.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGCAGGATTGTTTGATAAGCAAGCGGATTTGTACTTGGATTCCAGGCCAACTTATCCGACGGAATGGTATTCCAAGCTAGCAGATCTCACCCCTCACCACACTCTCGCTTGGGATGTCGGAACCGGCAACGGCCAAGCCGCTGTCAGCGTTGCTGAGCACTATGAACAAGTAATTGGAACCGATGTGAGTGAAGCTCAGTTGCAGCGTGCAATGCCACATCCTCGTGTTCGATATGTCCACACACCGTTAACCATTACAGACGACGAATTAATAGCATTGATCGGGGGCGAAAATTCAGTCGACCTGGTGACTGTGGCTCAAGCTGTGCACTGGTTCGACCTGCCAAAGTTTTACAATCTTGTTCCGCGGCTTCTGAGAAAGCCAGGAGGTGTGTTGGCCGTTTGGTGCTACAATGACATTGAAGTTTGTCCGGAATTTGATCCTATTATGAAGCGATTTCATGACACAACCCTGCCCTTTTGGGACCGTAACATCCAGTACATCTTTGATGGTTACAAGACGCTTCCATTTCCTTTTCAGAGTGTTGGTTTCGGATGCGAGGGGAGTCCATTGCCGCTGGACATTCCAAAGAAGCTGTCGTTCCAAGGGTTTTTGAAGATGGTAGGGTCATGGTCTGTGGTCATTACAGCCAAACACCAAGGCGTGGATTTGTTACCGGAAAAGCTGGTTGAAGAGTTTGAGGGTGCTTGGGGTGGCTCTAAATTGGTCAGGTCAGTCACCTACAAGGCTTTTATGCTTGCCGGAAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

259

Amino Acids

28.99

Weight (kDa)

5.59

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 39 - 134 7.7e-10 Methyltransferase domain
Methyltransf_25 PF13649 39 - 129 4.3e-09 Methyltransferase domain
Methyltransf_12 PF08242 39 - 133 1.3e-07 Methyltransferase domain
Methyltransf_31 PF13847 39 - 138 2.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 667
AccI GTMKAC 1 cut(s) 303
AccII CGCG 1 cut(s) 370
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 5 cut(s) 26, 141, 368, 370, 578
AclWI GGATC 1 cut(s) 437
AcoI YGGCCR 2 cut(s) 133, 399
AcsI RAATTY 2 cut(s) 295, 437
AfaI GTAC 2 cut(s) 35, 503
AfiI CCNNNNNNNGG 1 cut(s) 667
AgsI TTSAA 3 cut(s) 425, 619, 695
AjnI CCWGG 3 cut(s) 44, 306, 385
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 5 cut(s) 79, 197, 326, 598, 688
AluI AGCT 5 cut(s) 79, 197, 326, 598, 688
Alw21I GWGCWC 2 cut(s) 162, 333
Alw44I GTGCAC 1 cut(s) 329
AlwI GGATC 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 3 cut(s) 47, 133, 399
ApaLI GTGCAC 1 cut(s) 329
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 2 cut(s) 295, 437
ArsI GACNNNNNNTTYG 4 cut(s) 285, 317, 584, 616
AseI ATTAAT 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 487
AsuHPI GGTGA 4 cut(s) 82, 89, 322, 733
AsuNHI GCTAGC 1 cut(s) 79
AvaII GGWCC 1 cut(s) 487
BaeGI GKGCMC 1 cut(s) 333
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 162, 333
BbvI GCAGC 1 cut(s) 217
BccI CCATC 2 cut(s) 506, 616
BceAI ACGGC 2 cut(s) 148, 386
BciT130I CCWGG 3 cut(s) 46, 308, 387
BfaI CTAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 351
BglII AGATCT 1 cut(s) 85
BisI GCNGC 4 cut(s) 141, 206, 371, 578
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 4 cut(s) 142, 207, 372, 579
Bme1390I CCNGG 3 cut(s) 46, 308, 387
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 487
BmiI GGNNCC 3 cut(s) 124, 181, 488
BmrFI CCNGG 3 cut(s) 46, 308, 387
BmsI GCATC 1 cut(s) 548
BmtI GCTAGC 1 cut(s) 83
BplI GAGNNNNNCTC 2 cut(s) 181, 213
Bpu1102I GCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 306
BsaJI CCNNGG 3 cut(s) 368, 607, 661
BsaWI WCCGGW 2 cut(s) 433, 679
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 338, 499
Bse3DI GCAATG 2 cut(s) 219, 572
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 3 cut(s) 46, 308, 387
BseDI CCNNGG 3 cut(s) 368, 607, 661
BseGI GGATG 4 cut(s) 121, 220, 495, 563
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMI GCAATG 2 cut(s) 219, 572
BseMII CTCAG 3 cut(s) 147, 212, 368
BseNI ACTGG 2 cut(s) 338, 499
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 1 cut(s) 217
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 49, 135, 401
BsiHKAI GWGCWC 2 cut(s) 162, 333
BsiSI CCGG 4 cut(s) 126, 434, 680, 765
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 3 cut(s) 49, 135, 401
Bsp1286I GDGCHC 2 cut(s) 162, 333
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 3 cut(s) 85, 282, 442
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 5 cut(s) 26, 141, 368, 370, 578
BspANI GGCC 3 cut(s) 49, 135, 401
BspCNI CTCAG 3 cut(s) 148, 211, 369
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 370
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 3 cut(s) 124, 181, 488
BspMI ACCTGC 1 cut(s) 351
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 1 cut(s) 437
BsrDI GCAATG 2 cut(s) 219, 572
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 338, 499
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 3 cut(s) 368, 607, 661
BssMI GATC 3 cut(s) 85, 282, 442
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 607, 661
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 3 cut(s) 46, 308, 387
Bst4CI ACNGT 3 cut(s) 249, 316, 491
Bst6I CTCTTC 1 cut(s) 690
BstC8I GCNNGC 4 cut(s) 24, 81, 210, 762
BstDEI CTNAG 3 cut(s) 156, 198, 377
BstDSI CCRYGG 1 cut(s) 368
BstF5I GGATG 4 cut(s) 121, 220, 495, 563
BstFNI CGCG 1 cut(s) 370
BstKTI GATC 3 cut(s) 88, 285, 445
BstMBI GATC 3 cut(s) 85, 282, 442
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNI CCWGG 3 cut(s) 46, 308, 387
BstSCI CCNGG 3 cut(s) 44, 306, 385
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BsuRI GGCC 3 cut(s) 49, 135, 401
BtgI CCRYGG 1 cut(s) 368
BtsCI GGATG 4 cut(s) 121, 220, 495, 563
BtsIMutI CAGTG 1 cut(s) 331
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 4 cut(s) 24, 81, 210, 762
CciI TCATGA 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 487
Cfr42I CCGCGG 1 cut(s) 371
CseI GACGC 1 cut(s) 532
CsiI ACCWGGT 1 cut(s) 306
Csp6I GTAC 2 cut(s) 34, 502
CviAII CATG 2 cut(s) 464, 633
CviQI GTAC 2 cut(s) 34, 502
DdeI CTNAG 3 cut(s) 156, 198, 377
DpnI GATC 3 cut(s) 87, 284, 444
DpnII GATC 3 cut(s) 85, 282, 442
EaeI YGGCCR 2 cut(s) 133, 399
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
Eco130I CCWWGG 2 cut(s) 607, 661
Eco47I GGWCC 1 cut(s) 487
EcoRII CCWGG 3 cut(s) 44, 306, 385
EcoT14I CCWWGG 2 cut(s) 607, 661
ErhI CCWWGG 2 cut(s) 607, 661
FaeI CATG 2 cut(s) 467, 636
FaiI YATR 6 cut(s) 165, 237, 452, 465, 634, 758
FaqI GGGAC 1 cut(s) 500
FatI CATG 2 cut(s) 463, 632
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 4 cut(s) 141, 206, 371, 578
FokI GGATG 4 cut(s) 128, 207, 482, 570
Fsp4HI GCNGC 4 cut(s) 141, 206, 371, 578
FspBI CTAG 1 cut(s) 80
GluI GCNGC 4 cut(s) 141, 206, 371, 578
HaeIII GGCC 3 cut(s) 49, 135, 401
HapII CCGG 4 cut(s) 126, 434, 680, 765
HgaI GACGC 1 cut(s) 532
Hin1II CATG 2 cut(s) 467, 636
HincII GTYRAC 2 cut(s) 252, 304
HindII GTYRAC 2 cut(s) 252, 304
HinfI GANTC 2 cut(s) 41, 568
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 4 cut(s) 126, 434, 680, 765
HphI GGTGA 4 cut(s) 82, 89, 322, 733
Hpy166II GTNNAC 4 cut(s) 241, 252, 304, 331
Hpy188I TCNGA 5 cut(s) 60, 122, 378, 543, 557
Hpy188III TCNNGA 2 cut(s) 434, 464
Hpy8I GTNNAC 4 cut(s) 241, 252, 304, 331
Hpy99I CGWCG 2 cut(s) 64, 269
HpyCH4III ACNGT 3 cut(s) 249, 316, 491
HpyCH4V TGCA 3 cut(s) 205, 212, 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 156, 198, 377
Hsp92II CATG 2 cut(s) 467, 636
Kpn2I TCCGGA 1 cut(s) 433
KspAI GTTAAC 1 cut(s) 252
KspI CCGCGG 1 cut(s) 371
Kzo9I GATC 3 cut(s) 85, 282, 442
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 548
MabI ACCWGGT 1 cut(s) 306
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 5 cut(s) 310, 491, 515, 675, 739
MalI GATC 3 cut(s) 87, 284, 444
MboI GATC 3 cut(s) 85, 282, 442
MboII GAAGA 2 cut(s) 631, 707
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 2 cut(s) 162, 333
MluCI AATT 5 cut(s) 174, 269, 295, 437, 725
MlyI GAGTC 1 cut(s) 577
MmeI TCCRAC 2 cut(s) 83, 100
MnlI CCTC 5 cut(s) 105, 234, 383, 556, 697
MroI TCCGGA 1 cut(s) 433
MseI TTAA 3 cut(s) 251, 272, 775
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 3 cut(s) 143, 370, 580
MspI CCGG 4 cut(s) 126, 434, 680, 765
MspR9I CCNGG 3 cut(s) 46, 308, 387
MvaI CCWGG 3 cut(s) 46, 308, 387
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 1 cut(s) 214
NdeII GATC 3 cut(s) 85, 282, 442
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 2 cut(s) 467, 636
NlaIV GGNNCC 3 cut(s) 124, 181, 488
NmuCI GTSAC 2 cut(s) 310, 739
PagI TCATGA 1 cut(s) 463
PfeI GAWTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 4 cut(s) 142, 207, 372, 579
PleI GAGTC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 576
PshBI ATTAAT 1 cut(s) 272
Psp6I CCWGG 3 cut(s) 44, 306, 385
PspGI CCWGG 3 cut(s) 44, 306, 385
PspN4I GGNNCC 3 cut(s) 124, 181, 488
PspPI GGNCC 1 cut(s) 487
PsuI RGATCY 1 cut(s) 85
RsaI GTAC 2 cut(s) 35, 503
RsaNI GTAC 2 cut(s) 34, 502
RseI CAYNNNNRTG 1 cut(s) 225
SacII CCGCGG 1 cut(s) 371
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 3 cut(s) 251, 272, 775
SatI GCNGC 4 cut(s) 141, 206, 371, 578
Sau3AI GATC 3 cut(s) 85, 282, 442
Sau96I GGNCC 1 cut(s) 487
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 3 cut(s) 46, 308, 387
SduI GDGCHC 2 cut(s) 162, 333
SexAI ACCWGGT 1 cut(s) 306
SfaNI GCATC 1 cut(s) 548
Sfr303I CCGCGG 1 cut(s) 371
SgrBI CCGCGG 1 cut(s) 371
SinI GGWCC 1 cut(s) 487
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 5 cut(s) 174, 269, 295, 437, 725
SsiI CCGC 5 cut(s) 26, 141, 368, 370, 578
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 3 cut(s) 44, 306, 385
StyI CCWWGG 2 cut(s) 607, 661
TaaI ACNGT 3 cut(s) 249, 316, 491
TaqI TCGA 3 cut(s) 232, 303, 339
TasI AATT 5 cut(s) 174, 269, 295, 437, 725
TatI WGTACW 2 cut(s) 33, 501
TauI GCSGC 3 cut(s) 143, 373, 580
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 3 cut(s) 251, 272, 775
Tru9I TTAA 3 cut(s) 251, 272, 775
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 2 cut(s) 310, 739
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 2 cut(s) 310, 739
TspDTI ATGAA 3 cut(s) 180, 452, 467
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 667
VneI GTGCAC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 487
VspI ATTAAT 1 cut(s) 272
XapI RAATTY 2 cut(s) 295, 437
XmiI GTMKAC 1 cut(s) 303
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.