Rw1G011580

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001059
Physical Location & Seq
Forward (+)
25967685 .. 25968697
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011580.1

Sequence Viewer

Length: 828 bp
ATGGCACGTCCATTTGACAAGCAGGAAGAGATATACGTGGAAGCTGGGCCAACGTATCAGGCAGAGGTATACGTGGAAGCTCGGCCAACGTATCCCAAGGAATGGTATTCAAAGCTGGCTGCTCTCACCCCACAACACTCTTTAGCTTGGGATGTTGGCACTGGCAGTGGCCAAGCTACCGTCGGTCTTTTAGAGCATTACGACCAAGTAGTTGGAAGTGATATAAGTGAAGCACAATTGAACCATGCCATCCAGCACCCTCGAGTTCGATACGTTCACACTCCAACATCAATCTCAGACGATGAAATGGTTGACTTAATAGGGGGCGGTCATGAAAACTCAGTTGATTTGATTACAGTGGCTACTGCCGTTCACTGGTTTGATCTGCCACGCTTCTACTCGCTTGTCAAACGTCTTCTGCGTAAACCAGGAGGTATAATTGCTGTTTGGTCCTACAGCAGCATGGACAACATGGTCGTAAGCCCTGACTTTGATCTTGCAATGAAGCGTTTACACGAAAATTGTCTTCCATTTTGGGACCCGAGGACAAAGTACGCATTTGAGGGTTATAGGACACTTCCTTTTCCATTTGAGAGCGTTGGATTGGGTCGTGAAGGTGAACCTATCACACTTGAAATACCAAGGGAATTGTCATTTCAGGGGGTTGAGAGGATGTTGAGGTCTTACTCTGTAGTCACTACGGCTAAAGACCAGGGTGTGGATTTGTTGTCTGAAGATGTGATTAAAGAACTTCAAAGAGCTTGGGGTGGGCATGATCTGATTAGATCAGTTACAATGAAGGCATGTATGCTTGTGGGCAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

275

Amino Acids

31.02

Weight (kDa)

5.38

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 51 - 148 3.5e-09 Methyltransferase domain
Methyltransf_25 PF13649 51 - 144 1.8e-08 Methyltransferase domain
Methyltransf_12 PF08242 51 - 146 1.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 826
AasI GACNNNNNNGTC 1 cut(s) 473
AccB7I CCANNNNNTGG 2 cut(s) 102, 718
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 327
AcoI YGGCCR 2 cut(s) 83, 169
AcuI CTGAAG 1 cut(s) 753
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 3 cut(s) 102, 375, 718
AgsI TTSAA 4 cut(s) 111, 241, 635, 755
AjiI CACGTC 1 cut(s) 8
AjnI CCWGG 2 cut(s) 427, 711
AluBI AGCT 6 cut(s) 44, 80, 115, 146, 176, 761
AluI AGCT 6 cut(s) 44, 80, 115, 146, 176, 761
Ama87I CYCGRG 2 cut(s) 261, 541
AoxI GGCC 3 cut(s) 47, 83, 169
ApeKI GCWGC 2 cut(s) 119, 459
AspS9I GGNCC 3 cut(s) 47, 450, 538
AsuHPI GGTGA 2 cut(s) 118, 629
AvaI CYCGRG 2 cut(s) 261, 541
AvaII GGWCC 2 cut(s) 450, 538
BalI TGGCCA 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 407, 518
BbvI GCAGC 2 cut(s) 106, 471
BccI CCATC 1 cut(s) 257
BceAI ACGGC 2 cut(s) 353, 717
BciT130I CCWGG 2 cut(s) 429, 713
BciVI GTATCC 1 cut(s) 102
BfmI CTRYAG 2 cut(s) 454, 690
BfuI GTATCC 1 cut(s) 102
BisI GCNGC 2 cut(s) 120, 460
BlsI GCNGC 2 cut(s) 121, 461
Bme1390I CCNGG 2 cut(s) 429, 713
Bme18I GGWCC 2 cut(s) 450, 538
BmeT110I CYCGRG 2 cut(s) 261, 541
BmgBI CACGTC 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 47, 450, 538
BmiI GGNNCC 2 cut(s) 539, 540
BmrFI CCNGG 2 cut(s) 429, 713
BpiI GAAGAC 2 cut(s) 407, 518
BsaAI YACGTR 2 cut(s) 37, 73
BsaJI CCNNGG 4 cut(s) 96, 542, 641, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 375, 718
Bse1I ACTGG 2 cut(s) 166, 380
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 2 cut(s) 429, 713
BseDI CCNNGG 4 cut(s) 96, 542, 641, 712
BseGI GGATG 3 cut(s) 157, 249, 678
BseLI CCNNNNNNNGG 3 cut(s) 102, 375, 718
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 309, 354
BseNI ACTGG 2 cut(s) 166, 380
BseXI GCAGC 2 cut(s) 106, 471
BseYI CCCAGC 1 cut(s) 44
BshFI GGCC 3 cut(s) 49, 85, 171
BsiHKCI CYCGRG 2 cut(s) 261, 541
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 3 cut(s) 102, 375, 718
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 3 cut(s) 49, 85, 171
BsoBI CYCGRG 2 cut(s) 261, 541
Bsp143I GATC 4 cut(s) 382, 493, 775, 785
BspACI CCGC 1 cut(s) 327
BspANI GGCC 3 cut(s) 49, 85, 171
BspCNI CTCAG 2 cut(s) 308, 353
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 539, 540
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 166, 380
BssECI CCNNGG 4 cut(s) 96, 542, 641, 712
BssMI GATC 4 cut(s) 382, 493, 775, 785
BssNAI GTATAC 1 cut(s) 70
BssT1I CCWWGG 2 cut(s) 96, 641
Bst1107I GTATAC 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 429, 713
Bst4CI ACNGT 2 cut(s) 181, 358
Bst6I CTCTTC 1 cut(s) 21
BstBAI YACGTR 2 cut(s) 37, 73
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 295, 340
BstF5I GGATG 3 cut(s) 157, 249, 678
BstKTI GATC 4 cut(s) 385, 496, 778, 788
BstMBI GATC 4 cut(s) 382, 493, 775, 785
BstNI CCWGG 2 cut(s) 429, 713
BstNSI RCATGY 1 cut(s) 807
BstSCI CCNGG 2 cut(s) 427, 711
BstSFI CTRYAG 2 cut(s) 454, 690
BstV1I GCAGC 2 cut(s) 106, 471
BstV2I GAAGAC 2 cut(s) 407, 518
BstXI CCANNNNNNTGG 1 cut(s) 212
BstZ17I GTATAC 1 cut(s) 70
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 3 cut(s) 49, 85, 171
BtrI CACGTC 1 cut(s) 8
BtsCI GGATG 3 cut(s) 157, 249, 678
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 4 cut(s) 159, 172, 363, 373
Cac8I GCNNGC 1 cut(s) 117
CciI TCATGA 1 cut(s) 331
Cfr13I GGNCC 3 cut(s) 47, 450, 538
Csp6I GTAC 1 cut(s) 553
CviAII CATG 6 cut(s) 245, 332, 463, 472, 773, 804
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 2 cut(s) 295, 340
DpnI GATC 4 cut(s) 384, 495, 777, 787
DpnII GATC 4 cut(s) 382, 493, 775, 785
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 2 cut(s) 83, 169
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 96, 641
Eco47I GGWCC 2 cut(s) 450, 538
Eco57I CTGAAG 1 cut(s) 753
Eco88I CYCGRG 2 cut(s) 261, 541
EcoO109I RGGNCCY 1 cut(s) 538
EcoRII CCWGG 2 cut(s) 427, 711
EcoT14I CCWWGG 2 cut(s) 96, 641
ErhI CCWWGG 2 cut(s) 96, 641
FaeI CATG 6 cut(s) 248, 335, 466, 475, 776, 807
FaqI GGGAC 1 cut(s) 551
FatI CATG 6 cut(s) 244, 331, 462, 471, 772, 803
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 120, 460
FokI GGATG 3 cut(s) 164, 236, 685
Fsp4HI GCNGC 2 cut(s) 120, 460
GluI GCNGC 2 cut(s) 120, 460
GsaI CCCAGC 1 cut(s) 48
HaeIII GGCC 3 cut(s) 49, 85, 171
Hin1II CATG 6 cut(s) 248, 335, 466, 475, 776, 807
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HphI GGTGA 2 cut(s) 118, 629
Hpy166II GTNNAC 7 cut(s) 70, 277, 313, 373, 425, 512, 620
Hpy188I TCNGA 3 cut(s) 298, 733, 780
Hpy188III TCNNGA 2 cut(s) 332, 611
Hpy8I GTNNAC 7 cut(s) 70, 277, 313, 373, 425, 512, 620
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 608, 793
HpyCH4III ACNGT 2 cut(s) 181, 358
HpyCH4IV ACGT 7 cut(s) 7, 36, 53, 72, 89, 273, 412
HpyCH4V TGCA 1 cut(s) 500
HpyF3I CTNAG 2 cut(s) 295, 340
HpySE526I ACGT 7 cut(s) 7, 36, 53, 72, 89, 273, 412
Hsp92II CATG 6 cut(s) 248, 335, 466, 475, 776, 807
KflI GGGWCCC 1 cut(s) 538
Kzo9I GATC 4 cut(s) 382, 493, 775, 785
Lsp1109I GCAGC 2 cut(s) 106, 471
MaeII ACGT 7 cut(s) 7, 36, 53, 72, 89, 273, 412
MaeIII GTNAC 2 cut(s) 694, 790
MalI GATC 4 cut(s) 384, 495, 777, 787
MboI GATC 4 cut(s) 382, 493, 775, 785
MboII GAAGA 4 cut(s) 38, 407, 518, 746
MfeI CAATTG 1 cut(s) 236
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 5 cut(s) 236, 438, 520, 647, 821
MluNI TGGCCA 1 cut(s) 171
MmeI TCCRAC 3 cut(s) 193, 308, 580
MnlI CCTC 7 cut(s) 58, 270, 425, 537, 556, 663, 672
Mox20I TGGCCA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 171
MseI TTAA 2 cut(s) 317, 744
Msp20I TGGCCA 1 cut(s) 171
MspR9I CCNGG 2 cut(s) 429, 713
MunI CAATTG 1 cut(s) 236
MvaI CCWGG 2 cut(s) 429, 713
NdeII GATC 4 cut(s) 382, 493, 775, 785
NlaIII CATG 6 cut(s) 248, 335, 466, 475, 776, 807
NlaIV GGNNCC 2 cut(s) 539, 540
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 1 cut(s) 807
PaeR7I CTCGAG 1 cut(s) 261
PagI TCATGA 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 418
PflMI CCANNNNNTGG 2 cut(s) 102, 718
PkrI GCNGC 2 cut(s) 121, 461
Ppu21I YACGTR 2 cut(s) 37, 73
PpuMI RGGWCCY 1 cut(s) 538
PsiI TTATAA 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 538
Psp6I CCWGG 2 cut(s) 427, 711
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 2 cut(s) 427, 711
PspN4I GGNNCC 2 cut(s) 539, 540
PspPI GGNCC 3 cut(s) 47, 450, 538
PspPPI RGGWCCY 1 cut(s) 538
PspXI VCTCGAGB 1 cut(s) 261
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
SaqAI TTAA 2 cut(s) 317, 744
SatI GCNGC 2 cut(s) 120, 460
Sau3AI GATC 4 cut(s) 382, 493, 775, 785
Sau96I GGNCC 3 cut(s) 47, 450, 538
ScrFI CCNGG 2 cut(s) 429, 713
SfcI CTRYAG 2 cut(s) 454, 690
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 450, 538
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 5 cut(s) 236, 438, 520, 647, 821
SsiI CCGC 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 427, 711
StyI CCWWGG 2 cut(s) 96, 641
TaaI ACNGT 2 cut(s) 181, 358
TaiI ACGT 7 cut(s) 10, 39, 56, 75, 92, 276, 415
TaqI TCGA 2 cut(s) 262, 268
TaqII GACCGA 1 cut(s) 173
TasI AATT 5 cut(s) 236, 438, 520, 647, 821
Tru1I TTAA 2 cut(s) 317, 744
Tru9I TTAA 2 cut(s) 317, 744
TscAI CASTG 4 cut(s) 166, 172, 363, 380
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 2 cut(s) 119, 459
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 4 cut(s) 318, 348, 518, 812
TspRI CASTG 4 cut(s) 166, 172, 363, 380
Van91I CCANNNNNTGG 2 cut(s) 102, 718
VpaK11BI GGWCC 2 cut(s) 450, 538
XceI RCATGY 1 cut(s) 807
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.