RchiOBHm_Chr7g0198261

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001059
Physical Location & Seq
Reverse (-)
16103921 .. 16105419
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17739

Sequence Viewer

Length: 684 bp
ATGGATTTGAAGCTGGCCGGTCTCACCCCCGAACACTCTTTAGCTTGGGATCTTTCAGAGCATTACGAGCAAGTAGTGGGAAGCGACATCAGTGGAGAACAATTAAAACATGCCATCCAACATCCTCGAATTCGATACATTCATACTCCAGCATCAATCTCAGAGGATGAAATGGTCAACTTAATAGCCGGTGGTCATGAAAATTCAGTGGACTTGGTTACAGTGGCGGAAGCTGTTCACTGGTTTGATCTTCCTCATTTCTATTCACTCGTCAGACGCCTTCTGCGCAAACCAGGAGGCGTAATTGCAGTTTGGGGCTACAACGACATGGTAGTAAGCCCCGATTTTGATCTTTTAATGAAGCGTCACCACGAAAAGATGTTACCATATTATGACCCGAAATCAAGCTACTTATGGGAGGGTTATAGGACACTTCCATTTCCATTTGAGAGTGTTGGATTAGGTCGTGAAGGAGAGCCTATGGCACTTGAAATACATAAGGAACTTTCATTTCAGGGGCTTCTGAAGCTTCTGAGGTCTTCCTCTGCAGTCACTACGGCTAAAGACAAGGGTGTGGATTTGTTGTCTGAAGAGGTGATTGAAGAGATTCAAGGAGTTTGGGGTAGGCCTGATCTGGTTAGATCTGTTACGTACAAGGCTTTTATGCTTGCTGGCAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

227

Amino Acids

25.69

Weight (kDa)

5.64

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 287
AciI CCGC 1 cut(s) 227
AclWI GGATC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 2 cut(s) 129, 202
AcuI CTGAAG 2 cut(s) 545, 609
AcyI GRCGYC 1 cut(s) 277
AfaI GTAC 1 cut(s) 653
AgsI TTSAA 4 cut(s) 10, 491, 602, 611
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 5 cut(s) 13, 44, 233, 408, 529
AluI AGCT 5 cut(s) 13, 44, 233, 408, 529
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 57
AoxI GGCC 2 cut(s) 15, 626
ApoI RAATTY 2 cut(s) 129, 202
Asp700I GAANNNNTTC 2 cut(s) 234, 606
AspLEI GCGC 1 cut(s) 288
AsuHPI GGTGA 3 cut(s) 16, 359, 607
BbsI GAAGAC 1 cut(s) 531
BccI CCATC 1 cut(s) 122
BceAI ACGGC 1 cut(s) 573
BciT130I CCWGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 546
BglII AGATCT 1 cut(s) 641
Bme1390I CCNGG 1 cut(s) 294
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 26
Bse118I RCCGGY 2 cut(s) 17, 188
Bse1I ACTGG 1 cut(s) 245
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 294
BseGI GGATG 3 cut(s) 114, 121, 172
BseJI GATNNNNATC 1 cut(s) 348
BseMII CTCAG 2 cut(s) 174, 524
BseNI ACTGG 1 cut(s) 245
BshFI GGCC 2 cut(s) 17, 628
BsiSI CCGG 2 cut(s) 18, 189
BsmAI GTCTC 1 cut(s) 26
BsnI GGCC 2 cut(s) 17, 628
Bso31I GGTCTC 1 cut(s) 26
Bsp143I GATC 5 cut(s) 49, 247, 349, 631, 641
BspACI CCGC 1 cut(s) 227
BspANI GGCC 2 cut(s) 17, 628
BspCNI CTCAG 2 cut(s) 173, 525
BspHI TCATGA 1 cut(s) 196
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 26
BsrFI RCCGGY 2 cut(s) 17, 188
BsrI ACTGG 1 cut(s) 245
BssAI RCCGGY 2 cut(s) 17, 188
BssMI GATC 5 cut(s) 49, 247, 349, 631, 641
BssNI GRCGYC 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 585, 597
BstACI GRCGYC 1 cut(s) 277
BstBAI YACGTR 1 cut(s) 651
BstC8I GCNNGC 3 cut(s) 15, 669, 673
BstDEI CTNAG 2 cut(s) 160, 533
BstF5I GGATG 3 cut(s) 114, 121, 172
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 5 cut(s) 52, 250, 352, 634, 644
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 5 cut(s) 49, 247, 349, 631, 641
BstMWI GCNNNNNNNGC 3 cut(s) 67, 285, 526
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 292
BstSFI CTRYAG 1 cut(s) 546
BstSNI TACGTA 1 cut(s) 651
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 2 cut(s) 49, 641
BstYI RGATCY 2 cut(s) 49, 641
BsuRI GGCC 2 cut(s) 17, 628
BtsCI GGATG 3 cut(s) 114, 121, 172
BtsIMutI CAGTG 4 cut(s) 97, 213, 228, 238
Cac8I GCNNGC 3 cut(s) 15, 669, 673
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 288
Cfr10I RCCGGY 2 cut(s) 17, 188
CseI GACGC 2 cut(s) 285, 353
Csp6I GTAC 1 cut(s) 652
CviAII CATG 3 cut(s) 110, 197, 328
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 2 cut(s) 160, 533
DpnI GATC 5 cut(s) 51, 249, 351, 633, 643
DpnII GATC 5 cut(s) 49, 247, 349, 631, 641
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 585, 597
EarI CTCTTC 2 cut(s) 585, 597
EciI GGCGGA 1 cut(s) 242
Eco105I TACGTA 1 cut(s) 651
Eco147I AGGCCT 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 26
Eco57I CTGAAG 2 cut(s) 545, 609
EcoRI GAATTC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 292
FaeI CATG 3 cut(s) 113, 200, 331
FatI CATG 3 cut(s) 109, 196, 327
FokI GGATG 3 cut(s) 101, 108, 179
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 1 cut(s) 287
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 2 cut(s) 17, 628
HapII CCGG 2 cut(s) 18, 189
HgaI GACGC 2 cut(s) 285, 353
HhaI GCGC 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 3 cut(s) 113, 200, 331
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 1 cut(s) 607
HpaII CCGG 2 cut(s) 18, 189
HphI GGTGA 3 cut(s) 16, 359, 607
Hpy166II GTNNAC 3 cut(s) 178, 211, 238
Hpy188I TCNGA 6 cut(s) 58, 163, 275, 525, 534, 589
Hpy188III TCNNGA 2 cut(s) 197, 467
Hpy8I GTNNAC 3 cut(s) 178, 211, 238
HpyAV CCTTC 2 cut(s) 290, 464
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 650
HpyCH4V TGCA 2 cut(s) 308, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 285, 526
HpyF3I CTNAG 2 cut(s) 160, 533
HpySE526I ACGT 1 cut(s) 650
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 3 cut(s) 113, 200, 331
HspAI GCGC 1 cut(s) 286
Kzo9I GATC 5 cut(s) 49, 247, 349, 631, 641
LweI GCATC 1 cut(s) 161
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 5 cut(s) 217, 365, 381, 550, 646
MalI GATC 5 cut(s) 51, 249, 351, 633, 643
MboI GATC 5 cut(s) 49, 247, 349, 631, 641
MboII GAAGA 4 cut(s) 242, 531, 602, 614
MflI RGATCY 2 cut(s) 49, 641
MluCI AATT 4 cut(s) 101, 129, 202, 303
MmeI TCCRAC 2 cut(s) 142, 436
MnlI CCTC 8 cut(s) 135, 157, 264, 290, 412, 528, 553, 586
MroXI GAANNNNTTC 2 cut(s) 234, 606
MseI TTAA 3 cut(s) 104, 182, 356
MspI CCGG 2 cut(s) 18, 189
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 3 cut(s) 67, 285, 526
NdeII GATC 5 cut(s) 49, 247, 349, 631, 641
NlaIII CATG 3 cut(s) 113, 200, 331
NmuCI GTSAC 2 cut(s) 365, 550
NsbI TGCGCA 1 cut(s) 287
NspI RCATGY 1 cut(s) 113
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 628
PdmI GAANNNNTTC 2 cut(s) 234, 606
PfeI GAWTC 1 cut(s) 607
Ppu21I YACGTR 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PstI CTGCAG 1 cut(s) 550
PsuI RGATCY 2 cut(s) 49, 641
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
SaqAI TTAA 3 cut(s) 104, 182, 356
Sau3AI GATC 5 cut(s) 49, 247, 349, 631, 641
ScrFI CCNGG 1 cut(s) 294
SetI ASST 9 cut(s) 15, 46, 235, 410, 466, 531, 539, 597, 653
SfaNI GCATC 1 cut(s) 161
SfcI CTRYAG 1 cut(s) 546
SnaBI TACGTA 1 cut(s) 651
Sse9I AATT 4 cut(s) 101, 129, 202, 303
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 1 cut(s) 227
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 1 cut(s) 292
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 653
TaqI TCGA 2 cut(s) 127, 133
TasI AATT 4 cut(s) 101, 129, 202, 303
TfiI GAWTC 1 cut(s) 607
Tru1I TTAA 3 cut(s) 104, 182, 356
Tru9I TTAA 3 cut(s) 104, 182, 356
TscAI CASTG 4 cut(s) 97, 213, 228, 245
TseFI GTSAC 2 cut(s) 365, 550
Tsp45I GTSAC 2 cut(s) 365, 550
TspDTI ATGAA 5 cut(s) 131, 183, 213, 374, 498
TspRI CASTG 4 cut(s) 97, 213, 228, 245
XapI RAATTY 2 cut(s) 129, 202
XceI RCATGY 1 cut(s) 113
XmnI GAANNNNTTC 2 cut(s) 234, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.