AT4G22970

Peptidase family C50

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000800
Physical Location & Seq
Reverse (-)
12033437 .. 12043728
10292 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22970.1

Sequence Viewer

Length: 6543 bp
ATGGCGTCCTCTGGCGACGACCTCCGTCTTCTCTCTCTCATCGACGTCGGCGATAATGTCTTCTCTTCCTTCTCCGATTACCTTAAACCTTTCTCTACTCTATCCACTTCCCGGAAAAAACAAGACCGAGCAACCACGATTCGAGCCCTAGCTAAGCAATTCCTTCCTTTCCTCAACAAATCGATCTCTCTCCTACCGAAACGTCTCTCCGTCGCGAATTCTGATAAGGAAGCTCGTGAATCAGCATTAGATCTGTTCCGTGCTTATGAACTTTGTTTGGACTGTTTAGAATTGGTCTCTGCTCAATTAGCTTGTAAGCCTCACACTGTTCAATCTCAGAGGCTAAGGATGATTCATTGTCTCGATGTTTGGGGTTTGTATGAGAATGTGTATACTGAAGCGTTTAAGGTTCTGGAGAAGCTAAGAGGCTCTGATAGCAAATCCCGGAAGTCCCGGTTGTTGCCGGAGGTTCAGGATGGAGATGCGGAGATGGCTTTAGTTGTTGTTGATGCTGTGGCGGCCATCTTTAGGGCTGTGGCAATGAGTCAGCAGTTAGATGATAAGCGGTATCGGAAAGTTCTTCTTTTGCTTGAGGAAGTCGGAGGCTGGTTAAGGGTCTTAGATGCTAAGGTGTATGAAAAGTTGCACAGAGCGATGGTGACAAGTATGGGTAAATGTGCTGTTTCTCTCGTTAGAGAAGCTGAGCGTTTCAATGGGGATTTAGTGATTTCTTTCTGCGACTTAACTGTGAAGGAACATTATAAATCTGCATTATCAAAAGATCGAGTTTATAAGTTTGCTCGCGAGGTGCTTTCTGTTCTGTTTGGATTTAAGGATAGAAAAATGTCAGTGACCATTGACATTTCAATGTCTGTGTTGCGCAGCTTATCTTGCCAATTTGAGGATGAAAGTAATGAGAATTTAATGGAGTTTTTTGACCTAGTCGATTATTGCGCTCACAAGTTTCGAGCAGCAGGAGACATGTACTGTGCAAAAGTTTCAAAAAAATTGAATGAGATGGCAGCTATTTTCGTAGAGGCAATACCCCAACTTAATTTGGTTCTTAGACTGTATTCGACCGGGCTGTCCATCACGGTTTGCAATTCCAAGCTCGGAGAAATTAAGCTAGAAGATTCAACAGATGATTGGAAGATTCAAGCTATGTTTGATGATGATGCTAGATGGCAAAGCTTAGTTTCTTTACTCGGCATGGTTGATAGTTACTCAGGTGATGAGGGCAATCAGACTGGTTCATCATCGATTGGTGGGCATAGGAACTACAACAATAAAACACATGATAGCTGTAAAGACAGGAACAAGATTACTTGTTGGCCACAATACGTAGATGCCTTGAAATTCTTGTGCCAACCGCTTGCAGACTTTATATATTCTGTGAAAAGAAAGATTGTGCTGGAGACAGAAATGTCATGCGCCTCTGCTCATCTAATTACTATTCACGATGCCTTTCTCCAGTTTTGTGATGGCTGTCTCTTCCTTCAGAGGTGCACGTCTGACAAGGGAGATAGAGAAATTGCCAATAACAAAGCTTTCTTAAACGCGGCTATGGGTGCTTTCATTGTCTCATTGAGAACCCAACTTAAATTGGAGATAAGTGCCCATCTAGTTGAGGATGTAATTGGAAGCCCATGGATCCAATCTCAAGAACTCAAGTATCTAATTGCGACGCTGTACAATATTGGTATCGTTTTGTACAGAAATAAGGAGCTAAACAAGGCTTGTGAGGCGCTTAAGCTGTGCTCCAAGGTATCATGGAGATGTGTTGAACTACATTGTCATATGTTTGTAAATCAGTCTAGTTCATCTGATAATGACTTGTCAGAAGATGCCATTATGGATTTTGTGGGCGAAGCATGTAATAGGTGTGCATTCTACCTGGATATACTCCAGAAATGTAGCAGACGTAAGATTAGACAGAATATTGTTCATATTCTAGAAAATTGGTTGTCAGCTGAACATCTGATTAGACGGTTACCAGGCCCTGAGGCAATTGTAAAGCAATGGGTTAAGATAGAGCGGGAATGTCACACGGATCTGGATGCGGCAGGTTCTTGTACAACTTTGTACTCATTGTTATCATCTTCTCAAAAAAAGTCAAAGCGGGGTATCGGAAAAATCCTTGAGCAGGAGCTTCTTGCCTATGACAGAGTGTTACCTTTAAGGTCAAATCTAGGTCAACAAACGCGAATCAAGATAGCAGATATCCTCTTGAAGGATGTTTATGTCACTGAGGATATGCATATAGAGAGAGCAAGAATTTTGATATGGAAAGCACGAATGACAAGGACATCTGGAACTGAACATATAACCGAGTGCATTTGTTTTCTGTCTGAAGCAATCTCTATATTGGGTGAGTTACATCATGGGCCAAATGAAGAGGGTTCTCCATCTTCTCACATGTTACCTATTGCGTATTGCTTGAGAGCTTTTTGTACCCAGGAAGCTGACCCAAACTCGAAGAAAGTCTTTCAAGATATTAGCACTTCACTAAATCTCTGGCTAAGGATTCTTAGCCTAGATGATAGTGGAGACAGTCTACCAACAGAGAATATAATTCCGTTACTGTATAATATGATTGATTTGATGTCAGTGAAGGGATGTACAGAGCTCCATCATCATATATACCAACTGATTTTCAGATTGTTTAAATGGAAGAATGTCAAATTGGAGGTCTGCCTTGCAATGCTGTGGGAATGTAGAAGGCTTAGTCATGCCTTATGCCCTTCCCCCATAAGTGATGCCTTTATCCAGACCTTATCAGAGAATTGTGCTGACAAATCCACATGCATTGATTTCTGGATGGATTGCCTGAAAGATTCAAAAGCAAAGTTAATTGGGTTCCAGCAGAACTTCCATGATTTACATAATAAGGATGAAGGTCCCTTTCAATCAGACATCACAATTGACGATATTAAAGATGCAGCATCAGAACTCATCTCCAGTGCATCACTCTCTGGTAATTCATCTTTTGCAGCTGCATATCTCTATTATGATCTCTGTGAAAGGCTCATATCATTTGGAAAACTTTCTGAGGCTCTTTCATATGCAAAAGAAGCCTATAGGATAAGGACTCTTATATTTCAAGATAAATTTAAGTATACAGCTGAGAAGCATATCGAAAAACACAATGAAGATGGGAAAATATCAGAAATACGGACTTTTAGCATAAAAAATTTCCAAGTCTACAGATTGTTAGCAACTGATTTTTGGCCATGTGGAAATTTTCTCTGGGACATCAATCGCTGCTACCTAAGTCCTTGGAGTGTACTCCAATGTTATTTGGAAAGCACCCTTCAGGTTGGAATTCTGAATGAGCTCATAGGGAATGGACTGGAGGCAGAAACCATTCTATCATGGGGGAAAGCCTTTTCATGCTCGCAAAGTCTATTCCCTTTTGTAGTTGCATTTTCTTCTGCCTTAGGAAATCTATACCACAAAAAGCAGTGTCTGGATCTGGCAGAAAAGGAACTCCAAAATGCAAAAGAGATATTAATTGCTAATCAGCGAGATTTTTCTTGCGTAAAGTGCAAACTGAAGTTGGAAGTTACATTGGATAAACAACTTGGAGACATATCTCGGAAACAAATTGATAGAGTTTCACAGACAGATGGATTCTTGCATGCTGAAAGCTTGTTTAGTGCTGCCCTGGGAAAATTCTGTTGCTCAGCATGGAAAAGCTGCATTAGATCACATGGGGAAGAAATTGCTGAGGAGATAGTGATTGATAGAAATGGAGGGGAAGGTTTAGGACATAATTCAAGTAAAACAAAGCTCAGTATAAAGGAGCCACCAGGAAACAGAGGCTCCAGGAGGGGTGGAAGAGCTAACAAAACTTGTTTGTCAAAGGATCAGGATTTGATATCTGAGCCAACATCAAGGTTGACTCGATCTATGCGTCACTCACTTAGAGAGCAATGCCAAAACCGTTCTAATGTGCCTGAAGTTGTTTCAAAGAAGCCTAATTTATGTGATAGATCTGTTGGTTCTAGGGGTGAAAGGGTTTTGTTGGACACGAGTAATGCACTCCCTGGCTTTTGTATTTGCTACAAAGAAAAACGTCAGCAGTGCCTCTCGGAAGAAGTAACAGAATCTGGGTCTCTCAACAATTTAGTAAGTTTGAAATGGGAACTCTGCCATAGGAAGCTTGCGTCCTCAATACTTGTTAGCCTTGGAAAATGTTTGGGAGACTCTGGTAGAATTCATCTAGCTCATGAGGCTTTACTGCATAGTATCTCTGTTTTATTCAAAAGCACCTGGTCCAGCCACAACCAGCCTTCTGTCAGTCAGTTGCTGGAATTTATCGGGAAAGAAGTTACAAGAGATGTATTTGCCGTTGATCGTGCAATAATATTATACAACTTGTGCTGGTTGAATTTGCGGAATTACCACTGCAGGAAGAGTAGGTCTATCTGCTGTGATCTGTTTCATATCCCTTTCACAAAACTAGTGTCATGGTTAATGTTAGCATTTGTACTCAGCGGAGAGGTTCCAATATTATTTCAAAAGGTTTCAAGATTGCTTGCCTCTTTATATCTGCTTTCTTCCTCAAATTCTGAATTCACATTCGAATCTGATGGAAATGAGCTGTCAGCAAGTCATTGGGTCTCATTTTTCCATCAAGCCTCGCTTGGTACCCATCTCAGTTACCATTTCATTTCAAATTTGAGCCAAAAACACAAATCACAGTGCCTCTCAGATAAAGAGTGCACAGAGGCTACTTGCTCGAGTTGCATGGTTCCTGAGGATTTGGACTTACCCAGGCTTGCTCCTGACCGTACTCAAGATCTTGTACAATTCGCTAAAGAATTTTTCATCAATCTTCCAAGCTCAACAATCATCTGCATTAGTTTGCTTGGAGGTGCTTTAAACCAATTGTTACAGGAGCTAATGCATATTCGTTCACCTGTTTGTGCCTGGGTGCTCATATCACGCCTAAACCCAGAAAGTCAACCGGTTGCCACACTTTTACCTGTAGATTCGATTGTAGAAGACATGTCAGATGACAGTGCAAATCTTAGCTCTACCGAAGCGACTCAAGTCAAGAGTTTAAAGGGGCCCTGGCTTTGTCCATGGGGTACCACCGTGGTTGATGAGGTAGCTCCAGCATTTAAATCAATATTGGAGGAAAGCCACTCATCTTCTTCTACTACTGAAGAAGACACAATAGAGAGTAGGGGCCTGTGGTGGAAAAAGAGAAAGAAACTTAATCATCGTCTGGGAATATTCCTAAGGAATCTAGAAGCTTCCTGGCTGGGTCCCTGGAGGTGTCTGCTTCTCGGAGAATGGTCGAACTACAAATTGCCTGACTCAGCGCAGAAAAAGCTGGTAAATGATCTCAAGTCCAAGTGCAAAATGGAAGTAAATGAGATGCTTTTGAAGGTTATTCTTGGAGGTGGGACGGATAACTTTAAAGGAGAAGCATGCGTTGCTCAGCTTTCTTTAAGAAATGGTTGCTATGTAGGTAGAGGGGGATATCTTTATGAAGAAGATAGCTGTAAAACTCCTACTGCTGCGTCTAATATATCTGAAAGTAGGCATGAATTGGCCTTAAAACTGATACATGACGCAGCAAGCAAACTAGGGCAACAAGATGGACATGAAAATAGAGAACCTATCATTCTTGTGTTAGATCCCGAGGTTCAGATGCTTCCTTGGGAGAATATTCCAATACTAAGGAAACAGGAGGTGTACCGCATGCCTTCCGTAGGTTGCATATCTGCCGTGCTAAAGAAGCGCAGTCTTCAAGGAGAGCCAGCAAAATCTCATGTTGCTTCCTTCCCTCTTATTGATCCGCTAGATTCATTTTACTTGTTGAATCCTGGTGGTGATCTCACCGATACACAAGTCACGTTTGAGAGTTGGTTCAGAGATCAAAACTTTGAGGGTAAAGCTGGGTCAGAACCAAGTGCTATAGAGTTGACAGAAGCATTAGAAACTCATGATCTCTTTCTATACTTTGGTCATGGAAGCGGAGCACAGTATATACCTAGGCGTGAAATAGAGAAGCTAGATAACTGTTCTGCAACTTTCTTAATGGGATGCAGTAGTGGTTCACTATGGCTAAAAGGCTGCTACATCCCACAAGGTGTACCACTTTCTTATCTCCTAGGTGGATCTCCAGCCATTGTGGCGACTCTATGGGACGTAACAGACCGAGACATTGATCGGTTTGGGAAAGCGTTGTTGGAAGCATGGTTGCAAGAGAGGTCAGATTCTTCTTCAGAGGGTGGCTGCAGCCAATGTGAGTCATTAGCTAATGACCTTGCAGCAATGACTCTAAAAGGCACCAAGAGGTCAAGGAAACCATCATCGCGTAACAAGCCAGCTCAATCAGATGTGGATGGGTCAGGGAAGATTGAGTGCAACCACAAGCATAGACGTAAAATCGGTTCATTCATAGCCGCGGCTCGAGATGCGTGCAACTTGCAATACTTGATTGGGGCTGCGCCTGTCTGTTATGGTGTGCCAACTGGTATCACTAGGAAAAAAGGAATTGATGCTCTTCTTCCTTCTTCATCTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2180

Amino Acids

244.84

Weight (kDa)

6.81

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 18 - 274 3.9e-98 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 282 - 469 1.2e-82 Separase-like, TPR repeats
Separin_C PF03568 1643 - 2060 1.4e-101 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 762, 792
AasI GACNNNNNNGTC 2 cut(s) 1084, 1423
AatII GACGTC 1 cut(s) 48
Acc16I TGCGCA 1 cut(s) 881
Acc36I ACCTGC 1 cut(s) 2054
Acc65I GGTACC 2 cut(s) 4649, 5093
AccB1I GGYRCC 3 cut(s) 4649, 5093, 6302
AccBSI CCGCTC 1 cut(s) 2035
AccI GTMKAC 4 cut(s) 392, 2554, 3116, 3201
AccII CGCG 6 cut(s) 215, 804, 1559, 2203, 6331, 6422
AclWI GGATC 8 cut(s) 1645, 1658, 2058, 3477, 3873, 5642, 5801, 6139
AcoI YGGCCR 3 cut(s) 519, 1331, 3227
AcuI CTGAAG 8 cut(s) 417, 1481, 2370, 3296, 3572, 3978, 5190, 6222
AcyI GRCGYC 2 cut(s) 5, 45
AdeI CACNNNGTG 1 cut(s) 6104
AfiI CCNNNNNNNGG 6 cut(s) 111, 528, 901, 2143, 2230, 5723
AflII CTTAAG 1 cut(s) 1748
AflIII ACRYGT 3 cut(s) 981, 2415, 5010
AgeI ACCGGT 1 cut(s) 4969
AhlI ACTAGT 1 cut(s) 4462
AjiI CACGTC 1 cut(s) 1509
AjuI GAANNNNNNNTTGG 4 cut(s) 2666, 2698, 6185, 6217
Alw21I GWGCWC 7 cut(s) 1508, 1760, 2628, 3336, 4727, 4941, 5995
Alw44I GTGCAC 2 cut(s) 1504, 4723
AlwI GGATC 8 cut(s) 1645, 1658, 2058, 3477, 3873, 5642, 5801, 6139
AlwNI CAGNNNCTG 4 cut(s) 2000, 3466, 4109, 4309
Ama87I CYCGRG 3 cut(s) 4741, 5651, 6426
AoxI GGCC 8 cut(s) 519, 1331, 1996, 2384, 3227, 5072, 5194, 5562
ApaI GGGCCC 1 cut(s) 5076
ApaLI GTGCAC 2 cut(s) 1504, 4723
ArsI GACNNNNNNTTYG 4 cut(s) 2673, 2705, 4451, 4483
AseI ATTAAT 1 cut(s) 3509
AsiGI ACCGGT 1 cut(s) 4969
Asp700I GAANNNNTTC 2 cut(s) 3043, 3363
Asp718I GGTACC 2 cut(s) 4649, 5093
AspA2I CCTAGG 2 cut(s) 6005, 6124
AspLEI GCGC 7 cut(s) 882, 956, 1433, 1747, 5332, 5754, 6466
AspS9I GGNCC 8 cut(s) 1997, 2384, 2897, 4275, 5072, 5073, 5194, 5273
AsuC2I CCSGG 4 cut(s) 112, 445, 454, 1081
AsuHPI GGTGA 7 cut(s) 670, 1241, 2381, 4022, 4911, 5842, 5855
AsuII TTCGAA 1 cut(s) 4584
AvaI CYCGRG 3 cut(s) 4741, 5651, 6426
AvaII GGWCC 3 cut(s) 2897, 4275, 5273
AvrII CCTAGG 2 cut(s) 6005, 6124
AxyI CCTNAGG 4 cut(s) 2001, 3436, 4758, 5246
BaeGI GKGCMC 4 cut(s) 1508, 1618, 4727, 5076
BaeI ACNNNNGTAYC 6 cut(s) 1655, 1688, 5085, 5118, 5847, 5880
BalI TGGCCA 2 cut(s) 1333, 3229
BamHI GGATCC 1 cut(s) 1650
BanI GGYRCC 3 cut(s) 4649, 5093, 6302
BanII GRGCYC 4 cut(s) 148, 2628, 3336, 5076
BauI CACGAG 2 cut(s) 234, 4030
BbsI GAAGAC 5 cut(s) 20, 52, 5013, 5181, 5750
Bbv12I GWGCWC 7 cut(s) 1508, 1760, 2628, 3336, 4727, 4941, 5995
BbvCI CCTCAGC 1 cut(s) 3726
BceAI ACGGC 2 cut(s) 4334, 5723
BcgI CGANNNNNNTGC 2 cut(s) 6417, 6451
BcnI CCSGG 4 cut(s) 112, 445, 454, 1081
BcuI ACTAGT 1 cut(s) 4462
BfmI CTRYAG 6 cut(s) 3076, 3202, 4408, 4989, 5928, 6250
BfoI RGCGCY 1 cut(s) 1748
BfrI CTTAAG 1 cut(s) 1748
BfuAI ACCTGC 1 cut(s) 2054
BglI GCCNNNNNGGC 1 cut(s) 6146
BglII AGATCT 3 cut(s) 250, 3992, 4801
BlnI CCTAGG 2 cut(s) 6005, 6124
BlpI GCTNAGC 4 cut(s) 153, 702, 3682, 5448
Bme18I GGWCC 3 cut(s) 2897, 4275, 5273
BmeT110I CYCGRG 3 cut(s) 4741, 5651, 6426
BmgBI CACGTC 1 cut(s) 1509
BmgT120I GGNCC 8 cut(s) 1997, 2384, 2897, 4275, 5072, 5073, 5194, 5273
BoxI GACNNNNGTC 2 cut(s) 24, 5054
BpiI GAAGAC 5 cut(s) 20, 52, 5013, 5181, 5750
Bpu10I CCTNAGC 4 cut(s) 344, 627, 2519, 3726
Bpu1102I GCTNAGC 4 cut(s) 153, 702, 3682, 5448
Bpu14I TTCGAA 1 cut(s) 4584
BpuEI CTTGAG 8 cut(s) 611, 1644, 1652, 2159, 2458, 4782, 5037, 5339
BpuMI CCSGG 4 cut(s) 112, 445, 454, 1081
Bsa29I ATCGAT 2 cut(s) 182, 1259
BsaAI YACGTR 1 cut(s) 1342
BsaHI GRCGYC 2 cut(s) 5, 45
BsaI GGTCTC 3 cut(s) 301, 4119, 4627
BsaWI WCCGGW 1 cut(s) 4969
BsaXI ACNNNNNCTCC 2 cut(s) 3806, 3836
Bsc4I CCNNNNNNNGG 6 cut(s) 111, 528, 901, 2143, 2230, 5723
Bse118I RCCGGY 1 cut(s) 4969
Bse1I ACTGG 5 cut(s) 1252, 1471, 2958, 3354, 6493
Bse21I CCTNAGG 4 cut(s) 2001, 3436, 4758, 5246
Bse3DI GCAATG 5 cut(s) 546, 2024, 2706, 3938, 6294
BseCI ATCGAT 2 cut(s) 182, 1259
BseLI CCNNNNNNNGG 6 cut(s) 111, 528, 901, 2143, 2230, 5723
BseMI GCAATG 5 cut(s) 546, 2024, 2706, 3938, 6294
BseNI ACTGG 5 cut(s) 1252, 1471, 2958, 3354, 6493
BseRI GAGGAG 1 cut(s) 3743
BseSI GKGCMC 4 cut(s) 1508, 1618, 4727, 5076
BseYI CCCAGC 2 cut(s) 5269, 5909
Bsh1236I CGCG 6 cut(s) 215, 804, 1559, 2203, 6331, 6422
Bsh1285I CGRYCG 1 cut(s) 1080
BshFI GGCC 8 cut(s) 521, 1333, 1998, 2386, 3229, 5074, 5196, 5564
BshNI GGYRCC 3 cut(s) 4649, 5093, 6302
BshTI ACCGGT 1 cut(s) 4969
BshVI ATCGAT 2 cut(s) 182, 1259
BsiEI CGRYCG 1 cut(s) 1080
BsiHKAI GWGCWC 7 cut(s) 1508, 1760, 2628, 3336, 4727, 4941, 5995
BsiHKCI CYCGRG 3 cut(s) 4741, 5651, 6426
BsiSI CCGG 6 cut(s) 112, 445, 454, 464, 1080, 4970
BslFI GGGAC 6 cut(s) 436, 2883, 3263, 5259, 5428, 6173
BslI CCNNNNNNNGG 6 cut(s) 111, 528, 901, 2143, 2230, 5723
BsmBI CGTCTC 1 cut(s) 209
BsmFI GGGAC 6 cut(s) 436, 2883, 3263, 5259, 5428, 6173
BsmI GAATGC 1 cut(s) 1886
BsnI GGCC 8 cut(s) 521, 1333, 1998, 2386, 3229, 5074, 5196, 5564
Bso31I GGTCTC 3 cut(s) 301, 4119, 4627
BsoBI CYCGRG 3 cut(s) 4741, 5651, 6426
Bsp119I TTCGAA 1 cut(s) 4584
Bsp120I GGGCCC 1 cut(s) 5072
Bsp1407I TGTACA 5 cut(s) 1689, 1710, 2072, 2618, 4807
Bsp1720I GCTNAGC 4 cut(s) 153, 702, 3682, 5448
Bsp19I CCATGG 2 cut(s) 1646, 5087
Bsp68I TCGCGA 2 cut(s) 215, 804
BspANI GGCC 8 cut(s) 521, 1333, 1998, 2386, 3229, 5074, 5196, 5564
BspDI ATCGAT 2 cut(s) 182, 1259
BspFNI CGCG 6 cut(s) 215, 804, 1559, 2203, 6331, 6422
BspHI TCATGA 2 cut(s) 4228, 5956
BspMAI CTGCAG 2 cut(s) 4412, 6254
BspMI ACCTGC 1 cut(s) 2054
BspPI GGATC 8 cut(s) 1645, 1658, 2058, 3477, 3873, 5642, 5801, 6139
BspQI GCTCTTC 2 cut(s) 3832, 6525
BspT104I TTCGAA 1 cut(s) 4584
BspT107I GGYRCC 3 cut(s) 4649, 5093, 6302
BspTI CTTAAG 1 cut(s) 1748
BspTNI GGTCTC 3 cut(s) 301, 4119, 4627
BsrBI CCGCTC 1 cut(s) 2035
BsrDI GCAATG 5 cut(s) 546, 2024, 2706, 3938, 6294
BsrFI RCCGGY 1 cut(s) 4969
BsrGI TGTACA 5 cut(s) 1689, 1710, 2072, 2618, 4807
BsrI ACTGG 5 cut(s) 1252, 1471, 2958, 3354, 6493
BssAI RCCGGY 1 cut(s) 4969
BssNAI GTATAC 2 cut(s) 393, 3117
BssNI GRCGYC 2 cut(s) 5, 45
BssSI CACGAG 2 cut(s) 234, 4030
BssT1I CCWWGG 8 cut(s) 1646, 1761, 3275, 4186, 5087, 5669, 6005, 6124
Bst1107I GTATAC 2 cut(s) 393, 3117
Bst2BI CACGAG 2 cut(s) 234, 4030
Bst6I CTCTTC 6 cut(s) 70, 1496, 2388, 3832, 4409, 6525
BstACI GRCGYC 2 cut(s) 5, 45
BstAFI CTTAAG 1 cut(s) 1748
BstAPI GCANNNNNTGC 1 cut(s) 5444
BstAUI TGTACA 5 cut(s) 1689, 1710, 2072, 2618, 4807
BstBAI YACGTR 1 cut(s) 1342
BstBI TTCGAA 1 cut(s) 4584
BstDSI CCRYGG 4 cut(s) 1646, 5087, 5100, 6420
BstEII GGTNACC 1 cut(s) 1989
BstENI CCTNNNNNAGG 3 cut(s) 2141, 2228, 5721
BstFNI CGCG 6 cut(s) 215, 804, 1559, 2203, 6331, 6422
BstH2I RGCGCY 1 cut(s) 1748
BstHHI GCGC 7 cut(s) 882, 956, 1433, 1747, 5332, 5754, 6466
BstMCI CGRYCG 1 cut(s) 1080
BstNSI RCATGY 8 cut(s) 985, 1875, 2419, 2805, 3641, 5014, 5442, 5716
BstPAI GACNNNNGTC 2 cut(s) 24, 5054
BstPI GGTNACC 1 cut(s) 1989
BstSFI CTRYAG 6 cut(s) 3076, 3202, 4408, 4989, 5928, 6250
BstSLI GKGCMC 4 cut(s) 1508, 1618, 4727, 5076
BstSNI TACGTA 1 cut(s) 1342
BstUI CGCG 6 cut(s) 215, 804, 1559, 2203, 6331, 6422
BstV2I GAAGAC 5 cut(s) 20, 52, 5013, 5181, 5750
BstX2I RGATCY 8 cut(s) 250, 1650, 2050, 3469, 3992, 4801, 5647, 6131
BstYI RGATCY 8 cut(s) 250, 1650, 2050, 3469, 3992, 4801, 5647, 6131
BstZ17I GTATAC 2 cut(s) 393, 3117
Bsu15I ATCGAT 2 cut(s) 182, 1259
Bsu36I CCTNAGG 4 cut(s) 2001, 3436, 4758, 5246
BsuRI GGCC 8 cut(s) 521, 1333, 1998, 2386, 3229, 5074, 5196, 5564
BsuTUI ATCGAT 2 cut(s) 182, 1259
BtgI CCRYGG 4 cut(s) 1646, 5087, 5100, 6420
BtgZI GCGATG 2 cut(s) 668, 6312
BtrI CACGTC 1 cut(s) 1509
BtsI GCAGTG 3 cut(s) 3467, 4088, 4405
BtuMI TCGCGA 2 cut(s) 215, 804
BveI ACCTGC 1 cut(s) 2054
CaiI CAGNNNCTG 4 cut(s) 2000, 3466, 4109, 4309
CciI TCATGA 2 cut(s) 4228, 5956
CfoI GCGC 7 cut(s) 882, 956, 1433, 1747, 5332, 5754, 6466
Cfr10I RCCGGY 1 cut(s) 4969
Cfr13I GGNCC 8 cut(s) 1997, 2384, 2897, 4275, 5072, 5073, 5194, 5273
Cfr42I CCGCGG 1 cut(s) 6423
ClaI ATCGAT 2 cut(s) 182, 1259
CseI GACGC 5 cut(s) 1693, 3902, 4155, 5520, 5591
CsiI ACCWGGT 1 cut(s) 4271
CspAI ACCGGT 1 cut(s) 4969
CspCI CAANNNNNGTGG 4 cut(s) 2788, 2823, 5086, 5121
DraI TTTAAA 5 cut(s) 2665, 4884, 5067, 5128, 5428
DraIII CACNNNGTG 1 cut(s) 6104
DrdI GACNNNNNNGTC 2 cut(s) 1084, 1423
DseDI GACNNNNNNGTC 2 cut(s) 1084, 1423
EaeI YGGCCR 3 cut(s) 519, 1331, 3227
Eam1104I CTCTTC 6 cut(s) 70, 1496, 2388, 3832, 4409, 6525
EarI CTCTTC 6 cut(s) 70, 1496, 2388, 3832, 4409, 6525
Ecl136II GAGCTC 2 cut(s) 2626, 3334
Eco105I TACGTA 1 cut(s) 1342
Eco130I CCWWGG 8 cut(s) 1646, 1761, 3275, 4186, 5087, 5669, 6005, 6124
Eco24I GRGCYC 4 cut(s) 148, 2628, 3336, 5076
Eco31I GGTCTC 3 cut(s) 301, 4119, 4627
Eco32I GATATC 3 cut(s) 2221, 3879, 5492
Eco47I GGWCC 3 cut(s) 2897, 4275, 5273
Eco53kI GAGCTC 2 cut(s) 2626, 3334
Eco57I CTGAAG 8 cut(s) 417, 1481, 2370, 3296, 3572, 3978, 5190, 6222
Eco81I CCTNAGG 4 cut(s) 2001, 3436, 4758, 5246
Eco88I CYCGRG 3 cut(s) 4741, 5651, 6426
Eco91I GGTNACC 1 cut(s) 1989
EcoICRI GAGCTC 2 cut(s) 2626, 3334
EcoNI CCTNNNNNAGG 3 cut(s) 2141, 2228, 5721
EcoO109I RGGNCCY 6 cut(s) 1997, 2897, 5072, 5073, 5194, 5273
EcoO65I GGTNACC 1 cut(s) 1989
EcoRI GAATTC 4 cut(s) 217, 3321, 4215, 4574
EcoRV GATATC 3 cut(s) 2221, 3879, 5492
EcoT14I CCWWGG 8 cut(s) 1646, 1761, 3275, 4186, 5087, 5669, 6005, 6124
EcoT22I ATGCAT 3 cut(s) 2259, 2807, 4911
EcoT38I GRGCYC 4 cut(s) 148, 2628, 3336, 5076
ErhI CCWWGG 8 cut(s) 1646, 1761, 3275, 4186, 5087, 5669, 6005, 6124
Esp3I CGTCTC 1 cut(s) 209
FalI AAGNNNNNCTT 6 cut(s) 567, 599, 2468, 2500, 4629, 4661
FaqI GGGAC 6 cut(s) 436, 2883, 3263, 5259, 5428, 6173
FauI CCCGC 2 cut(s) 2028, 2112
FauNDI CATATG 2 cut(s) 1797, 3061
FblI GTMKAC 4 cut(s) 392, 2554, 3116, 3201
FriOI GRGCYC 4 cut(s) 148, 2628, 3336, 5076
FspI TGCGCA 1 cut(s) 881
GlaI GCGC 7 cut(s) 881, 955, 1432, 1746, 5331, 5753, 6465
GsaI CCCAGC 2 cut(s) 5273, 5913
HaeII RGCGCY 1 cut(s) 1748
HaeIII GGCC 8 cut(s) 521, 1333, 1998, 2386, 3229, 5074, 5196, 5564
HapII CCGG 6 cut(s) 112, 445, 454, 464, 1080, 4970
HgaI GACGC 5 cut(s) 1693, 3902, 4155, 5520, 5591
HhaI GCGC 7 cut(s) 882, 956, 1433, 1747, 5332, 5754, 6466
Hin1I GRCGYC 2 cut(s) 5, 45
Hin6I GCGC 7 cut(s) 880, 954, 1431, 1745, 5330, 5752, 6464
HinP1I GCGC 7 cut(s) 880, 954, 1431, 1745, 5330, 5752, 6464
HincII GTYRAC 4 cut(s) 2195, 3900, 4967, 5937
HindII GTYRAC 4 cut(s) 2195, 3900, 4967, 5937
HindIII AAGCTT 5 cut(s) 1189, 1545, 3646, 4160, 5259
HpaII CCGG 6 cut(s) 112, 445, 454, 464, 1080, 4970
HphI GGTGA 7 cut(s) 670, 1241, 2381, 4022, 4911, 5842, 5855
Hpy99I CGWCG 5 cut(s) 20, 47, 50, 215, 1687
HpyCH4IV ACGT 9 cut(s) 45, 202, 1341, 1508, 1921, 4075, 5867, 6162, 6397
HpySE526I ACGT 9 cut(s) 45, 202, 1341, 1508, 1921, 4075, 5867, 6162, 6397
Hsp92I GRCGYC 2 cut(s) 5, 45
HspAI GCGC 7 cut(s) 880, 954, 1431, 1745, 5330, 5752, 6464
KflI GGGWCCC 1 cut(s) 5273
KpnI GGTACC 2 cut(s) 4653, 5097
KspI CCGCGG 1 cut(s) 6423
LguI GCTCTTC 2 cut(s) 3832, 6525
MabI ACCWGGT 1 cut(s) 4271
MaeII ACGT 9 cut(s) 45, 202, 1341, 1508, 1921, 4075, 5867, 6162, 6397
MbiI CCGCTC 1 cut(s) 2035
MfeI CAATTG 3 cut(s) 2007, 2919, 4889
MflI RGATCY 8 cut(s) 250, 1650, 2050, 3469, 3992, 4801, 5647, 6131
MlsI TGGCCA 2 cut(s) 1333, 3229
MluNI TGGCCA 2 cut(s) 1333, 3229
MlyI GAGTC 9 cut(s) 553, 3082, 3895, 4199, 5044, 5318, 6145, 6272, 6286
MmeI TCCRAC 5 cut(s) 580, 3298, 3537, 4005, 6183
Mox20I TGGCCA 2 cut(s) 1333, 3229
Mph1103I ATGCAT 3 cut(s) 2259, 2807, 4911
MroXI GAANNNNTTC 2 cut(s) 3043, 3363
MscI TGGCCA 2 cut(s) 1333, 3229
MslI CAYNNNNRTG 3 cut(s) 866, 1774, 5639
Msp20I TGGCCA 2 cut(s) 1333, 3229
MspA1I CMGCKG 5 cut(s) 1970, 2993, 3122, 4497, 6422
MspCI CTTAAG 1 cut(s) 1748
MspI CCGG 6 cut(s) 112, 445, 454, 464, 1080, 4970
MunI CAATTG 3 cut(s) 2007, 2919, 4889
Mva1269I GAATGC 1 cut(s) 1886
MvnI CGCG 6 cut(s) 215, 804, 1559, 2203, 6331, 6422
NciI CCSGG 4 cut(s) 112, 445, 454, 1081
NcoI CCATGG 2 cut(s) 1646, 5087
NdeI CATATG 2 cut(s) 1797, 3061
NmeAIII GCCGAG 1 cut(s) 1185
NmuCI GTSAC 6 cut(s) 658, 850, 2042, 2242, 3914, 5863
NruI TCGCGA 2 cut(s) 215, 804
NsbI TGCGCA 1 cut(s) 881
NsiI ATGCAT 3 cut(s) 2259, 2807, 4911
NspI RCATGY 8 cut(s) 985, 1875, 2419, 2805, 3641, 5014, 5442, 5716
NspV TTCGAA 1 cut(s) 4584
PaeI GCATGC 3 cut(s) 3641, 5442, 5716
PaeR7I CTCGAG 2 cut(s) 4741, 6426
PagI TCATGA 2 cut(s) 4228, 5956
PasI CCCWGGG 1 cut(s) 3664
PciI ACATGT 3 cut(s) 981, 2415, 5010
PciSI GCTCTTC 2 cut(s) 3832, 6525
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 3910
PctI GAATGC 1 cut(s) 1886
PdmI GAANNNNTTC 2 cut(s) 3043, 3363
PflFI GACNNNGTC 1 cut(s) 941
PfoI TCCNGGA 3 cut(s) 110, 443, 3824
PinAI ACCGGT 1 cut(s) 4969
PleI GAGTC 9 cut(s) 552, 3082, 3895, 4199, 5044, 5318, 6145, 6271, 6286
PpsI GAGTC 9 cut(s) 552, 3082, 3895, 4199, 5044, 5318, 6145, 6271, 6286
Ppu21I YACGTR 1 cut(s) 1342
PpuMI RGGWCCY 2 cut(s) 2897, 5273
PscI ACATGT 3 cut(s) 981, 2415, 5010
PshAI GACNNNNGTC 2 cut(s) 24, 5054
PshBI ATTAAT 1 cut(s) 3509
PsiI TTATAA 2 cut(s) 762, 792
Psp124BI GAGCTC 2 cut(s) 2628, 3336
Psp5II RGGWCCY 2 cut(s) 2897, 5273
PspEI GGTNACC 1 cut(s) 1989
PspFI CCCAGC 2 cut(s) 5269, 5909
PspOMI GGGCCC 1 cut(s) 5072
PspPI GGNCC 8 cut(s) 1997, 2384, 2897, 4275, 5072, 5073, 5194, 5273
PspPPI RGGWCCY 2 cut(s) 2897, 5273
PspXI VCTCGAGB 1 cut(s) 4741
PsrI GAACNNNNNNTAC 2 cut(s) 6391, 6423
PstI CTGCAG 2 cut(s) 4412, 6254
PstNI CAGNNNCTG 4 cut(s) 2000, 3466, 4109, 4309
PsuI RGATCY 8 cut(s) 250, 1650, 2050, 3469, 3992, 4801, 5647, 6131
PsyI GACNNNGTC 1 cut(s) 941
PvuII CAGCTG 3 cut(s) 1970, 2993, 3122
RruI TCGCGA 2 cut(s) 215, 804
RseI CAYNNNNRTG 3 cut(s) 866, 1774, 5639
SacI GAGCTC 2 cut(s) 2628, 3336
SacII CCGCGG 1 cut(s) 6423
SapI GCTCTTC 2 cut(s) 3832, 6525
Sau96I GGNCC 8 cut(s) 1997, 2384, 2897, 4275, 5072, 5073, 5194, 5273
SchI GAGTC 9 cut(s) 553, 3082, 3895, 4199, 5044, 5318, 6145, 6272, 6286
SexAI ACCWGGT 1 cut(s) 4271
SfcI CTRYAG 6 cut(s) 3076, 3202, 4408, 4989, 5928, 6250
Sfr274I CTCGAG 2 cut(s) 4741, 6426
Sfr303I CCGCGG 1 cut(s) 6423
SfuI TTCGAA 1 cut(s) 4584
SgrBI CCGCGG 1 cut(s) 6423
SinI GGWCC 3 cut(s) 2897, 4275, 5273
SlaI CTCGAG 2 cut(s) 4741, 6426
SmiI ATTTAAAT 1 cut(s) 5128
SmiMI CAYNNNNRTG 3 cut(s) 866, 1774, 5639
SnaBI TACGTA 1 cut(s) 1342
SpeI ACTAGT 1 cut(s) 4462
SphI GCATGC 3 cut(s) 3641, 5442, 5716
SspI AATATT 7 cut(s) 1696, 1939, 4368, 4512, 5136, 5241, 5680
SstI GAGCTC 2 cut(s) 2628, 3336
StyI CCWWGG 8 cut(s) 1646, 1761, 3275, 4186, 5087, 5669, 6005, 6124
SwaI ATTTAAAT 1 cut(s) 5128
TaiI ACGT 9 cut(s) 48, 205, 1344, 1511, 1924, 4078, 5870, 6165, 6400
TaqII GACCGA 2 cut(s) 141, 6186
TatI WGTACW 9 cut(s) 984, 1689, 1710, 2072, 2082, 2618, 3283, 4489, 4807
TauI GCSGC 5 cut(s) 521, 1562, 2063, 6422, 6425
TseFI GTSAC 6 cut(s) 658, 850, 2042, 2242, 3914, 5863
Tsp45I GTSAC 6 cut(s) 658, 850, 2042, 2242, 3914, 5863
TspGWI ACGGA 8 cut(s) 14, 199, 248, 2063, 2565, 3187, 5432, 5710
Tth111I GACNNNGTC 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 1748
VneI GTGCAC 2 cut(s) 1504, 4723
VpaK11BI GGWCC 3 cut(s) 2897, 4275, 5273
VspI ATTAAT 1 cut(s) 3509
XagI CCTNNNNNAGG 3 cut(s) 2141, 2228, 5721
XbaI TCTAGA 2 cut(s) 1951, 5254
XceI RCATGY 8 cut(s) 985, 1875, 2419, 2805, 3641, 5014, 5442, 5716
XcmI CCANNNNNNNNNTGG 2 cut(s) 1478, 5368
XhoI CTCGAG 2 cut(s) 4741, 6426
XmaJI CCTAGG 2 cut(s) 6005, 6124
XmiI GTMKAC 4 cut(s) 392, 2554, 3116, 3201
XmnI GAANNNNTTC 2 cut(s) 3043, 3363
ZraI GACGTC 1 cut(s) 46
Zsp2I ATGCAT 3 cut(s) 2259, 2807, 4911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.