MD15G1418900.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000800
Physical Location & Seq
Forward (+)
52022864 .. 52032443
9580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1418900.v1.1.491

Sequence Viewer

Length: 3861 bp
ATGAGCTCGAAAGGGGATGTCTTGGCGCGCCCTAGGCCCCTAGGAAAGAAAAAACCAGACATTTCACTCTCTCTTTCTCCACCGGCCATGGAGGAGACCGCCATCATTTCAAAACTTGAAGCCGCCAACACCGCCGGCCTTCATCCCCTCGTCGCCGCCTATCTTCAGCCGTTCACCGAACTCCAGAACCCCAAAAAAACCAAAAAATCAACCAAATCCCAAGACCACCAAACCCTCCTCCGCTCGCTCGCCAAGAAATTCCTCCCCTTCCTCAACCGCACGCTATCCCTTCTCCCCAAACGCCTCGCCGACCCCTCAAAGTTAGATGACAAATTTGCCCTCGAACTCTTCGATATTTACCGGCTCTGCCTCGACTGCCTGGCCGCCGTGTCCTCTGTGTTGTCCGCTTCGCCGCACTCGTTTCACTACCCGAGGGTGAGGATGGTGAGTTGCCTTGTGGCATGTGGGCGGTACAAGGATGCGGAAAGTGAGGGGTTTAGGGTTTTGGAGAGCCTTAAGGCGATTGAATGTGGGTCGAAGAAATCGGTGAAATCGTGTCGGAGATTTGTGCCGGATGTGGAGAAGGGGGGCGGAGATAAAGATTTTGGGTCTTTGGTGGGTGAAATTGTGGTGACGCTTGTGAAATGTGCGGCCATGAGTCAGAGTAAGGACAGTGAGGTGTTTGAGCGGGTGCTTTGTTTGGCTGAGGAGGTCATGCCGTGGTTCCGGGTATTAGATGCAAGCACATGTGAGAAGTTGCACAGGAATCTGGTTGCCTATCTGAGTAGGTGTACTCAATTTTTAGTTGGGGAGCTATCGGTGTTCAATGGGGATCTTGTGCAAAAGTTCTGCATTTTAACCATGACTGAGTATGCTAAGTCGCCAATGAAAGACACAATGGTCAAGTTTGCCCGTACGATATGTTCATCTTTATTCTTGTTTCAAGAGGATACATTCACACTCACTCGCATCTTATTTTGCTTGTTGGACTCCCTTGTCCACGAATGTAAGGCTGAAGTGGAAAATTCAGGAAAAGAGTTTGTTGAACTTATTGCTTATTGTGCCAAAAAATGTCAAACTACAAACACAAACCTTTGCGGTATTATTGGATCTCATTTAAATAAGTTAGCAGGTGATTTCCATCAGGCTGGAACACCTTTTCAGTTAATTCTGAGGGTTTATGCCACTGGATTGCACTTTGTTGATCGTAGTATGAAGTCAAAAGTTGGTCATTTTCAATCCTTTGAAGGTGCAATTAGAGTTTTGCTTGATGATGGGGACACAGGGAATCGCTTGTCCGGTTTGCTTGGTTCATTGAGAAGTTATTTCCAAATTGGCTGCAATGACGACTCTTTGTTATCCAATTCGCAATTGAGTTCGGACAGTGGGGACTCATTGAATCAAATGCAGAAGGATAGAAAGAACTATCTTCTGTGTTATTTTAATGCATTGAAATTCTTATGCCAGCCGCTTACAGAGTTTGTAAATTCAGGAAGGAAACAGATTATTACTAATAATGAAGCTGCTTCTGTTTCTACTGAAGTGTGCCATATCCAGGGTGCATTCCATCAGTTTTGTGACGTTTTTCTTTCTCTTAAAATGTACAGATGTACATATGAGGTGGATAGAGATGGATTTGATGGAAACAGCACCCTTGATGTTGCTCTGGCTGCTTTTACCCTCTCCATCATAACAAAGCTGAACATTCAGAAGAGTGTACAAATACTTGAAAATGTCATCACCAGTGCTTGGATTCAACCTCATGGTCTAAAGCATCTATATGTCTCTCTGTACAATACCGGGGTACATTTGTACAGGAACAAGGAGCTAAAAGAGGCATCACAGGCATTGAACTTATGTTGTAAGGCATCATGGACTCGTGTTATACATCTTTGTGAGATGTTTGTACACAAACAAAGGGCGTCCGAGGTTGATCTTGCTTTTCTTTTGGACGTTCTTAATGAATTGCAGAGTTATGATGCGAAAAGAACTTTATTAGAGAGCCTTGAAAATTGGTCCATTGCTGCAAACTTGTTTGGGAGGCTGCCAGGTCCTCTGGCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAAACGTTACAAGGATGTGAATGTTGAAGATGATGCTCCAACCTTATACAGTTTGTTATTGTCTTATAAGAAAGTCCCAAAGAAGATAAACGAAATCGTTTTGGAACAGGAACTTCTTGCTTATGAGGGAATGACTGCTGTGAACCCTAAGTTTTGTCAGAAAATGCAAATGAAAATTATAGATTTCCTGCTGAAAGATGTGTATGTCACACCAAATAGTTGGTTACAAAAATCAAGAATTTTGTTGAAAAAGGGAAGGGCATTAAGGCTTTCTGGAAGCAAAGGTCTGAAGGACTGTATTCAGTGTTTATCAGATGCAATATGTTTACTTAGTGAGATTTATGATGAAACCTGTACCCATGAAATTTCTCCTTGCCATCAATTAGCTGTGGCATATTGCTTACGTGCACTTTCTACCCAGGAAGCTGAACCCAACTCAAAGCGAGTGCTTGAAGATATCAGTGCTGCCATTAATCTATGGTTGGGCATTTCTACTCCTGCTAATTGCTCTCCGGCTGACAAGTGCTCCATGTTGTCTGAAAATACTATGTTGCTACTTTACAATGTCATTGATTTGTTATCCGCCAAGGGTTGCATGGACTTTCACAATGATATACATAAGCTTATGATTAGATTATTTAAATGGAGGAATGTCCCATTAGAGAAGTGCGTGGCCAGATTTTGGGAATGTAGGAGGATTAGCCATGCCCTTTGTGCCTCACCTGTAAATGAGACATTCATTATGAACTTATCAGATCATTGTGGTGAACTTTCAAAGTATGCTTTTTGGATAGATTCTCTAAAAGATTCCACACCATTACTATTAGTGTTCCAACATAGTTTCTCATTTCTGTTTCCAAACTTTTTCTCGGGGCCCATGGAATCATCAAAATTTGCTCCTGTATCGACTTGGTCTGCTTACATAGCTGGATATCTCTACTATGATTTATCGGAAAGACTTGTTTCAAATGGACGGTTAATTGAGGCTCTCTCATATGCAAAAGAAGCTCACAACTTACGTGCTAAACTATTTGGAGGGAAATTTATGTTCTCTTCTGAGCGACAGCCCAAAAAGTACAATGAAGGGGGTATCTGTCAGGAGTTAACATATAGCATTCATGATATGCATATGCAAAGATCAGTTGCTAGTGAAGTTTGGCTTTTTGATACTAGTTCATGTGACCTGGAAAGCTATTATCTGAGTCCATGGAATGCACTTCAATGTTATCTTGAGAGCACTCTTCAGGTAGGGGTTATCCTCGAAATAATTGGTAAAGGAGCTGAGGCCGAAGGTTTTTTACAATTTGGAAAAGCTTTTTCCTGCTCACAGAGCTTGCCACTATTTACAATTGTTTTTTCTACTGTCTTGGGAAAGCTTTACCACAAGCAGCAACTTTGGGATTTGGCGGAAAAGGAACTGCAAAGTGCCAAGCAATATTTTGGGGCTTGCAGCACAGATCTCTCTTGCATGAAATGCAGATTGTTGCTGGAAGCAACTGTTAATCAGAATCTTGGCGATTTATATCAAAGCATTTTTGAAAATACAAGAAGTACATCGTCAGATAAGTTGTCGCATGCTGAAAACCTGTACAAATCAGCCATTGCCATACTAAATCTTTCTGAGTGGAAAAATTCTGTTAGTTGTCCAGAAGAAGAGTGTGTCGAATGGACAATGCCTGGAAAAGCTAGTCTTAAAGACGTTGGATATTGTGCTAGCAGTATATATACTGTTTCTGAAGAAAAACAGCATGATAATAGAAAAAACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1287

Amino Acids

144.78

Weight (kDa)

7.78

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 32 - 311 4.9e-113 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 323 - 502 3.5e-56 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2157
AarI CACCTGC 1 cut(s) 1121
AasI GACNNNNNNGTC 2 cut(s) 899, 995
Acc36I ACCTGC 1 cut(s) 1121
AccB7I CCANNNNNTGG 2 cut(s) 1749, 2772
AccBSI CCGCTC 2 cut(s) 243, 688
AccII CGCG 1 cut(s) 28
AclI AACGTT 1 cut(s) 2095
AclWI GGATC 2 cut(s) 840, 1117
AcoI YGGCCR 4 cut(s) 84, 381, 651, 2763
AcuI CTGAAG 6 cut(s) 149, 1035, 1560, 2399, 3317, 3846
AcyI GRCGYC 1 cut(s) 1922
AfiI CCNNNNNNNGG 3 cut(s) 1555, 1749, 2772
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 746
AhlI ACTAGT 1 cut(s) 3260
AjnI CCWGG 6 cut(s) 378, 1554, 2047, 2508, 3273, 3766
Alw21I GWGCWC 4 cut(s) 8, 2500, 2618, 3329
Alw26I GTCTC 3 cut(s) 89, 1789, 2816
Alw44I GTGCAC 1 cut(s) 2496
AlwI GGATC 2 cut(s) 840, 1117
Ama87I CYCGRG 2 cut(s) 430, 2959
AoxI GGCC 8 cut(s) 35, 84, 136, 381, 651, 2763, 2963, 3375
ApaI GGGCCC 1 cut(s) 2967
ApaLI GTGCAC 1 cut(s) 2496
ApeKI GCWGC 8 cut(s) 1338, 1523, 1670, 2024, 2044, 2555, 3478, 3540
AscI GGCGCGCC 1 cut(s) 26
AseI ATTAAT 1 cut(s) 2562
AspA2I CCTAGG 2 cut(s) 32, 40
AspLEI GCGC 2 cut(s) 28, 30
AspS9I GGNCC 5 cut(s) 36, 2016, 2051, 2963, 2964
AsuC2I CCSGG 2 cut(s) 728, 1801
AsuNHI GCTAGC 1 cut(s) 3803
AvaI CYCGRG 2 cut(s) 430, 2959
AvaII GGWCC 2 cut(s) 2016, 2051
AvrII CCTAGG 2 cut(s) 32, 40
BaeGI GKGCMC 2 cut(s) 2500, 2967
BaeI ACNNNNGTAYC 2 cut(s) 1796, 1829
BalI TGGCCA 1 cut(s) 2765
BanII GRGCYC 2 cut(s) 8, 2967
BarI GAAGNNNNNNTAC 2 cut(s) 3372, 3404
BauI CACGAG 1 cut(s) 1878
Bbv12I GWGCWC 4 cut(s) 8, 2500, 2618, 3329
BbvCI CCTCAGC 2 cut(s) 705, 3372
BbvI GCAGC 8 cut(s) 1325, 1510, 1657, 2011, 2031, 2542, 3490, 3552
BceAI ACGGC 3 cut(s) 154, 371, 703
BciT130I CCWGG 6 cut(s) 380, 1556, 2049, 2510, 3275, 3768
BciVI GTATCC 1 cut(s) 943
BcnI CCSGG 2 cut(s) 728, 1801
BcoDI GTCTC 3 cut(s) 89, 1789, 2816
BcuI ACTAGT 1 cut(s) 3260
BfaI CTAG 6 cut(s) 33, 41, 3237, 3261, 3777, 3804
BfrI CTTAAG 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 1121
BfuI GTATCC 1 cut(s) 943
BglII AGATCT 1 cut(s) 3547
BlnI CCTAGG 2 cut(s) 32, 40
Bme1390I CCNGG 8 cut(s) 380, 728, 1556, 1801, 2049, 2510, 3275, 3768
Bme18I GGWCC 2 cut(s) 2016, 2051
BmeT110I CYCGRG 2 cut(s) 430, 2959
BmgT120I GGNCC 5 cut(s) 36, 2016, 2051, 2963, 2964
BmiI GGNNCC 4 cut(s) 38, 725, 2964, 2965
BmrFI CCNGG 8 cut(s) 380, 728, 1556, 1801, 2049, 2510, 3275, 3768
BmsI GCATC 9 cut(s) 469, 727, 978, 1783, 1847, 1877, 1969, 2113, 2395
BmtI GCTAGC 1 cut(s) 3807
BplI GAGNNNNNCTC 2 cut(s) 3065, 3097
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 2 cut(s) 705, 3372
BpuEI CTTGAG 1 cut(s) 3341
BpuMI CCSGG 2 cut(s) 728, 1801
BsaAI YACGTR 2 cut(s) 2495, 3110
BsaBI GATNNNNATC 2 cut(s) 1140, 3612
BsaHI GRCGYC 1 cut(s) 1922
BsaI GGTCTC 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 1298
BsaXI ACNNNNNCTCC 2 cut(s) 2600, 2630
Bsc4I CCNNNNNNNGG 3 cut(s) 1555, 1749, 2772
Bse118I RCCGGY 3 cut(s) 82, 134, 360
Bse1I ACTGG 2 cut(s) 1192, 1743
Bse3DI GCAATG 3 cut(s) 1348, 2019, 3690
Bse8I GATNNNNATC 2 cut(s) 1140, 3612
BseBI CCWGG 6 cut(s) 380, 1556, 2049, 2510, 3275, 3768
BseGI GGATG 6 cut(s) 22, 142, 447, 484, 580, 2110
BseJI GATNNNNATC 2 cut(s) 1140, 3612
BseLI CCNNNNNNNGG 3 cut(s) 1555, 1749, 2772
BseMI GCAATG 3 cut(s) 1348, 2019, 3690
BseMII CTCAG 8 cut(s) 696, 773, 858, 1163, 3138, 3281, 3363, 3702
BseNI ACTGG 2 cut(s) 1192, 1743
BsePI GCGCGC 1 cut(s) 26
BseRI GAGGAG 3 cut(s) 107, 227, 722
BseSI GKGCMC 2 cut(s) 2500, 2967
BseXI GCAGC 8 cut(s) 1325, 1510, 1657, 2011, 2031, 2542, 3490, 3552
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 8 cut(s) 37, 86, 138, 383, 653, 2765, 2965, 3377
BsiHKAI GWGCWC 4 cut(s) 8, 2500, 2618, 3329
BsiHKCI CYCGRG 2 cut(s) 430, 2959
BsiSI CCGG 8 cut(s) 83, 135, 361, 572, 727, 1299, 1800, 2603
BsiWI CGTACG 1 cut(s) 914
BslFI GGGAC 4 cut(s) 1292, 1403, 2150, 2729
BslI CCNNNNNNNGG 3 cut(s) 1555, 1749, 2772
BsmAI GTCTC 3 cut(s) 89, 1789, 2816
BsmFI GGGAC 4 cut(s) 1292, 1403, 2150, 2729
BsmI GAATGC 3 cut(s) 1562, 3204, 3307
BsnI GGCC 8 cut(s) 37, 86, 138, 383, 653, 2765, 2965, 3377
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 2 cut(s) 430, 2959
Bsp120I GGGCCC 1 cut(s) 2963
Bsp1286I GDGCHC 5 cut(s) 8, 2500, 2618, 2967, 3329
Bsp1407I TGTACA 7 cut(s) 1602, 1610, 1717, 1791, 1812, 1906, 3678
Bsp143I GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
Bsp19I CCATGG 3 cut(s) 87, 2967, 3296
BspANI GGCC 8 cut(s) 37, 86, 138, 383, 653, 2765, 2965, 3377
BspCNI CTCAG 8 cut(s) 697, 774, 859, 1164, 3139, 3282, 3364, 3703
BspFNI CGCG 1 cut(s) 28
BspHI TCATGA 1 cut(s) 3208
BspLI GGNNCC 4 cut(s) 38, 725, 2964, 2965
BspMI ACCTGC 1 cut(s) 1121
BspOI GCTAGC 1 cut(s) 3807
BspPI GGATC 2 cut(s) 840, 1117
BspTI CTTAAG 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 2 cut(s) 243, 688
BsrDI GCAATG 3 cut(s) 1348, 2019, 3690
BsrFI RCCGGY 3 cut(s) 82, 134, 360
BsrGI TGTACA 7 cut(s) 1602, 1610, 1717, 1791, 1812, 1906, 3678
BsrI ACTGG 2 cut(s) 1192, 1743
BssAI RCCGGY 3 cut(s) 82, 134, 360
BssHII GCGCGC 1 cut(s) 26
BssMI GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
BssNI GRCGYC 1 cut(s) 1922
BssSI CACGAG 1 cut(s) 1878
BssT1I CCWWGG 6 cut(s) 32, 40, 87, 2676, 2967, 3296
Bst2BI CACGAG 1 cut(s) 1878
Bst2UI CCWGG 6 cut(s) 380, 1556, 2049, 2510, 3275, 3768
Bst4CI ACNGT 8 cut(s) 674, 1385, 2141, 2387, 3066, 3454, 3589, 3820
Bst6I CTCTTC 5 cut(s) 353, 1706, 3148, 3336, 3738
BstACI GRCGYC 1 cut(s) 1922
BstAFI CTTAAG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 3564
BstAUI TGTACA 7 cut(s) 1602, 1610, 1717, 1791, 1812, 1906, 3678
BstBAI YACGTR 2 cut(s) 2495, 3110
BstDSI CCRYGG 4 cut(s) 87, 719, 2967, 3296
BstF5I GGATG 6 cut(s) 22, 142, 447, 484, 580, 2110
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 2 cut(s) 28, 30
BstKTI GATC 7 cut(s) 835, 1112, 1207, 1936, 2848, 3230, 3550
BstMAI GTCTC 3 cut(s) 89, 1789, 2816
BstMBI GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
BstNI CCWGG 6 cut(s) 380, 1556, 2049, 2510, 3275, 3768
BstNSI RCATGY 3 cut(s) 465, 750, 3668
BstSCI CCNGG 8 cut(s) 378, 726, 1554, 1799, 2047, 2508, 3273, 3766
BstSLI GKGCMC 2 cut(s) 2500, 2967
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 8 cut(s) 1325, 1510, 1657, 2011, 2031, 2542, 3490, 3552
BstX2I RGATCY 3 cut(s) 832, 1109, 3547
BstXI CCANNNNNNTGG 2 cut(s) 1148, 2310
BstYI RGATCY 3 cut(s) 832, 1109, 3547
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 8 cut(s) 37, 86, 138, 383, 653, 2765, 2965, 3377
BtgI CCRYGG 4 cut(s) 87, 719, 2967, 3296
BtsCI GGATG 6 cut(s) 22, 142, 447, 484, 580, 2110
BtsI GCAGTG 1 cut(s) 2078
BtsIMutI CAGTG 7 cut(s) 679, 1185, 1390, 1750, 2078, 2399, 2557
BveI ACCTGC 1 cut(s) 1121
CciI TCATGA 1 cut(s) 3208
CfoI GCGC 2 cut(s) 28, 30
Cfr10I RCCGGY 3 cut(s) 82, 134, 360
Cfr13I GGNCC 5 cut(s) 36, 2016, 2051, 2963, 2964
CseI GACGC 2 cut(s) 643, 1911
DpnI GATC 7 cut(s) 834, 1111, 1206, 1935, 2847, 3229, 3549
DpnII GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
DraI TTTAAA 2 cut(s) 1119, 2731
DrdI GACNNNNNNGTC 2 cut(s) 899, 995
DseDI GACNNNNNNGTC 2 cut(s) 899, 995
EaeI YGGCCR 4 cut(s) 84, 381, 651, 2763
Eam1104I CTCTTC 5 cut(s) 353, 1706, 3148, 3336, 3738
EarI CTCTTC 5 cut(s) 353, 1706, 3148, 3336, 3738
EciI GGCGGA 3 cut(s) 606, 2662, 3512
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 6 cut(s) 32, 40, 87, 2676, 2967, 3296
Eco24I GRGCYC 2 cut(s) 8, 2967
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 2 cut(s) 2548, 3023
Eco47I GGWCC 2 cut(s) 2016, 2051
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 6 cut(s) 149, 1035, 1560, 2399, 3317, 3846
Eco88I CYCGRG 2 cut(s) 430, 2959
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 3 cut(s) 36, 2051, 2963
EcoRII CCWGG 6 cut(s) 378, 1554, 2047, 2508, 3273, 3766
EcoRV GATATC 2 cut(s) 2548, 3023
EcoT14I CCWWGG 6 cut(s) 32, 40, 87, 2676, 2967, 3296
EcoT22I ATGCAT 2 cut(s) 1450, 3219
EcoT38I GRGCYC 2 cut(s) 8, 2967
ErhI CCWWGG 6 cut(s) 32, 40, 87, 2676, 2967, 3296
FalI AAGNNNNNCTT 4 cut(s) 3234, 3266, 3765, 3797
FaqI GGGAC 4 cut(s) 1292, 1403, 2150, 2729
FauI CCCGC 1 cut(s) 681
FauNDI CATATG 3 cut(s) 1615, 3085, 3219
FokI GGATG 6 cut(s) 29, 129, 454, 491, 587, 2117
FriOI GRGCYC 2 cut(s) 8, 2967
FspBI CTAG 6 cut(s) 33, 41, 3237, 3261, 3777, 3804
GlaI GCGC 2 cut(s) 27, 29
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 8 cut(s) 37, 86, 138, 383, 653, 2765, 2965, 3377
HapII CCGG 8 cut(s) 83, 135, 361, 572, 727, 1299, 1800, 2603
HgaI GACGC 2 cut(s) 643, 1911
HhaI GCGC 2 cut(s) 28, 30
Hin1I GRCGYC 1 cut(s) 1922
Hin6I GCGC 2 cut(s) 26, 28
HinP1I GCGC 2 cut(s) 26, 28
HincII GTYRAC 1 cut(s) 3195
HindII GTYRAC 1 cut(s) 3195
HindIII AAGCTT 3 cut(s) 2711, 3402, 3464
HpaI GTTAAC 1 cut(s) 3195
HpaII CCGG 8 cut(s) 83, 135, 361, 572, 727, 1299, 1800, 2603
Hpy99I CGWCG 1 cut(s) 155
HpyCH4III ACNGT 8 cut(s) 674, 1385, 2141, 2387, 3066, 3454, 3589, 3820
HpyCH4IV ACGT 6 cut(s) 1581, 1953, 2095, 2494, 3109, 3789
HpySE526I ACGT 6 cut(s) 1581, 1953, 2095, 2494, 3109, 3789
Hsp92I GRCGYC 1 cut(s) 1922
HspAI GCGC 2 cut(s) 26, 28
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 3195
Kzo9I GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
LmnI GCTCC 6 cut(s) 811, 1825, 2131, 2621, 2992, 3368
Lsp1109I GCAGC 8 cut(s) 1325, 1510, 1657, 2011, 2031, 2542, 3490, 3552
LweI GCATC 9 cut(s) 469, 727, 978, 1783, 1847, 1877, 1969, 2113, 2395
MaeI CTAG 6 cut(s) 33, 41, 3237, 3261, 3777, 3804
MaeII ACGT 6 cut(s) 1581, 1953, 2095, 2494, 3109, 3789
MaeIII GTNAC 6 cut(s) 631, 1577, 2096, 2296, 2313, 3269
MalI GATC 7 cut(s) 834, 1111, 1206, 1935, 2847, 3229, 3549
MbiI CCGCTC 2 cut(s) 243, 688
MboI GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
MfeI CAATTG 2 cut(s) 1370, 3438
MflI RGATCY 3 cut(s) 832, 1109, 3547
MhlI GDGCHC 5 cut(s) 8, 2500, 2618, 2967, 3329
MlsI TGGCCA 1 cut(s) 2765
MluNI TGGCCA 1 cut(s) 2765
MlyI GAGTC 6 cut(s) 667, 983, 1343, 1385, 1870, 3301
MmeI TCCRAC 5 cut(s) 539, 966, 2153, 2947, 3772
Mox20I TGGCCA 1 cut(s) 2765
Mph1103I ATGCAT 2 cut(s) 1450, 3219
MroNI GCCGGC 1 cut(s) 134
MscI TGGCCA 1 cut(s) 2765
MslI CAYNNNNRTG 4 cut(s) 1779, 1893, 2853, 3310
Msp20I TGGCCA 1 cut(s) 2765
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 8 cut(s) 83, 135, 361, 572, 727, 1299, 1800, 2603
MspR9I CCNGG 8 cut(s) 380, 728, 1556, 1801, 2049, 2510, 3275, 3768
MunI CAATTG 2 cut(s) 1370, 3438
Mva1269I GAATGC 3 cut(s) 1562, 3204, 3307
MvaI CCWGG 6 cut(s) 380, 1556, 2049, 2510, 3275, 3768
MvnI CGCG 1 cut(s) 28
NaeI GCCGGC 1 cut(s) 136
NciI CCSGG 2 cut(s) 728, 1801
NcoI CCATGG 3 cut(s) 87, 2967, 3296
NdeI CATATG 3 cut(s) 1615, 3085, 3219
NdeII GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
NgoMIV GCCGGC 1 cut(s) 134
NheI GCTAGC 1 cut(s) 3803
NlaIV GGNNCC 4 cut(s) 38, 725, 2964, 2965
NmuCI GTSAC 4 cut(s) 631, 1577, 2296, 3269
NsiI ATGCAT 2 cut(s) 1450, 3219
NspI RCATGY 3 cut(s) 465, 750, 3668
PaeI GCATGC 1 cut(s) 3668
PagI TCATGA 1 cut(s) 3208
PalAI GGCGCGCC 1 cut(s) 26
PaqCI CACCTGC 1 cut(s) 1121
PauI GCGCGC 1 cut(s) 26
PciI ACATGT 1 cut(s) 746
PcsI WCGNNNNNNNCGW 3 cut(s) 348, 416, 551
PctI GAATGC 3 cut(s) 1562, 3204, 3307
PdiI GCCGGC 1 cut(s) 136
PfeI GAWTC 8 cut(s) 766, 1288, 1399, 1753, 2885, 2897, 2972, 3598
Pfl23II CGTACG 1 cut(s) 914
PflFI GACNNNGTC 1 cut(s) 3001
PflMI CCANNNNNTGG 2 cut(s) 1749, 2772
PleI GAGTC 6 cut(s) 666, 983, 1343, 1385, 1870, 3300
PpsI GAGTC 6 cut(s) 666, 983, 1343, 1385, 1870, 3300
Ppu21I YACGTR 2 cut(s) 2495, 3110
PpuMI RGGWCCY 1 cut(s) 2051
PscI ACATGT 1 cut(s) 746
PshBI ATTAAT 1 cut(s) 2562
PsiI TTATAA 1 cut(s) 2157
Psp124BI GAGCTC 1 cut(s) 8
Psp1406I AACGTT 1 cut(s) 2095
Psp5II RGGWCCY 1 cut(s) 2051
Psp6I CCWGG 6 cut(s) 378, 1554, 2047, 2508, 3273, 3766
PspGI CCWGG 6 cut(s) 378, 1554, 2047, 2508, 3273, 3766
PspLI CGTACG 1 cut(s) 914
PspN4I GGNNCC 4 cut(s) 38, 725, 2964, 2965
PspOMI GGGCCC 1 cut(s) 2963
PspPI GGNCC 5 cut(s) 36, 2016, 2051, 2963, 2964
PspPPI RGGWCCY 1 cut(s) 2051
PsrI GAACNNNNNNTAC 2 cut(s) 907, 939
PsuI RGATCY 3 cut(s) 832, 1109, 3547
PsyI GACNNNGTC 1 cut(s) 3001
PteI GCGCGC 1 cut(s) 26
RseI CAYNNNNRTG 4 cut(s) 1779, 1893, 2853, 3310
SacI GAGCTC 1 cut(s) 8
Sau3AI GATC 7 cut(s) 832, 1109, 1204, 1933, 2845, 3227, 3547
Sau96I GGNCC 5 cut(s) 36, 2016, 2051, 2963, 2964
SchI GAGTC 6 cut(s) 667, 983, 1343, 1385, 1870, 3301
ScrFI CCNGG 8 cut(s) 380, 728, 1556, 1801, 2049, 2510, 3275, 3768
SduI GDGCHC 5 cut(s) 8, 2500, 2618, 2967, 3329
SfaNI GCATC 9 cut(s) 469, 727, 978, 1783, 1847, 1877, 1969, 2113, 2395
SgsI GGCGCGCC 1 cut(s) 26
SinI GGWCC 2 cut(s) 2016, 2051
SmiI ATTTAAAT 2 cut(s) 1119, 2731
SmiMI CAYNNNNRTG 4 cut(s) 1779, 1893, 2853, 3310
SmlI CTYRAG 2 cut(s) 515, 3320
SmoI CTYRAG 2 cut(s) 515, 3320
SpeI ACTAGT 1 cut(s) 3260
SphI GCATGC 1 cut(s) 3668
SspI AATATT 1 cut(s) 3527
SspMI CTAG 6 cut(s) 33, 41, 3237, 3261, 3777, 3804
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 8 cut(s) 378, 726, 1554, 1799, 2047, 2508, 3273, 3766
StyI CCWWGG 6 cut(s) 32, 40, 87, 2676, 2967, 3296
SwaI ATTTAAAT 2 cut(s) 1119, 2731
TaaI ACNGT 8 cut(s) 674, 1385, 2141, 2387, 3066, 3454, 3589, 3820
TaiI ACGT 6 cut(s) 1584, 1956, 2098, 2497, 3112, 3792
TaqI TCGA 8 cut(s) 8, 342, 351, 372, 536, 2996, 3351, 3753
TauI GCSGC 6 cut(s) 125, 158, 386, 415, 653, 1471
TfiI GAWTC 8 cut(s) 766, 1288, 1399, 1753, 2885, 2897, 2972, 3598
TscAI CASTG 7 cut(s) 679, 1192, 1390, 1750, 2078, 2399, 2557
TseFI GTSAC 4 cut(s) 631, 1577, 2296, 3269
TseI GCWGC 8 cut(s) 1338, 1523, 1670, 2024, 2044, 2555, 3478, 3540
Tsp45I GTSAC 4 cut(s) 631, 1577, 2296, 3269
TspRI CASTG 7 cut(s) 679, 1192, 1390, 1750, 2078, 2399, 2557
Tth111I GACNNNGTC 1 cut(s) 3001
Van91I CCANNNNNTGG 2 cut(s) 1749, 2772
Vha464I CTTAAG 1 cut(s) 515
VneI GTGCAC 1 cut(s) 2496
VpaK11BI GGWCC 2 cut(s) 2016, 2051
VspI ATTAAT 1 cut(s) 2562
XceI RCATGY 3 cut(s) 465, 750, 3668
XcmI CCANNNNNNNNNTGG 1 cut(s) 2683
XmaJI CCTAGG 2 cut(s) 32, 40
XspI CTAG 6 cut(s) 33, 41, 3237, 3261, 3777, 3804
Zsp2I ATGCAT 2 cut(s) 1450, 3219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.