Rroxscaffold_5G00332730

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000800
Physical Location & Seq
Reverse (-)
108200 .. 110721
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00332730.1

Sequence Viewer

Length: 381 bp
ATGAAATCCGAGGCGGGTCCCGTCATTGGGGCTTTGCTCGGAGAAGTGGTGTGGACACTAGCGAAGTATGCTTCGTTAATTCAAAGCAAGGAACACGAGGTGTATGAGTGGCTACTTTGTATGGTTGAGGAGTTGGGTCCTCGGTTTAGGTCAATGAATGCCACCTCCGACCAGCACACAGTGCCGATAGTTTCAGCTCGCCCTCGTCGACGTCTTCGTGGCCTTAGATCATCTATACGTCAAAGGAGGAAGGAGAATCGAAGCCGGAAAGCTGTCGGTATTTCCAACGGTTCTTCGACCGTTTCCCGTGACTCCGAGCATCGTCGGGGCTGCATGAGGTATAAAATTGGTATTCTCATCGTGCACCATATCTCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

126

Amino Acids

14.34

Weight (kDa)

10.85

Isoelectric Point (pI)

61.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 6 - 57 5.9e-07 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 208
AciI CCGC 1 cut(s) 14
AcyI GRCGYC 1 cut(s) 211
AdeI CACNNNGTG 2 cut(s) 100, 181
AfiI CCNNNNNNNGG 3 cut(s) 26, 27, 373
AgsI TTSAA 1 cut(s) 83
AluBI AGCT 2 cut(s) 197, 272
AluI AGCT 2 cut(s) 197, 272
Alw21I GWGCWC 1 cut(s) 366
Alw44I GTGCAC 1 cut(s) 362
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 1 cut(s) 330
AspS9I GGNCC 2 cut(s) 17, 137
AvaII GGWCC 2 cut(s) 17, 137
BaeGI GKGCMC 1 cut(s) 366
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 317
BfaI CTAG 1 cut(s) 59
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
Bme18I GGWCC 2 cut(s) 17, 137
BmgT120I GGNCC 2 cut(s) 17, 137
BmiI GGNNCC 3 cut(s) 18, 19, 138
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 211
BsaJI CCNNGG 2 cut(s) 9, 140
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 27, 373
BseDI CCNNGG 2 cut(s) 9, 140
BseLI CCNNNNNNNGG 3 cut(s) 26, 27, 373
BseRI GAGGAG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 1 cut(s) 317
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 1 cut(s) 3
BslI CCNNNNNNNGG 3 cut(s) 26, 27, 373
BsmFI GGGAC 1 cut(s) 3
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 222
BspLI GGNNCC 3 cut(s) 18, 19, 138
BssECI CCNNGG 2 cut(s) 9, 140
BssMI GATC 1 cut(s) 227
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 181, 290, 301
BstACI GRCGYC 1 cut(s) 211
BstAPI GCANNNNNTGC 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 1 cut(s) 224
BstKTI GATC 1 cut(s) 230
BstMBI GATC 1 cut(s) 227
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 2 cut(s) 68, 181
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 317
BstV2I GAAGAC 1 cut(s) 206
BsuRI GGCC 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 17, 137
CviAII CATG 1 cut(s) 334
CviJI RGCY 7 cut(s) 32, 112, 197, 222, 264, 272, 330
CviKI_1 RGCY 7 cut(s) 32, 112, 197, 222, 264, 272, 330
DdeI CTNAG 1 cut(s) 224
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
DraIII CACNNNGTG 2 cut(s) 100, 181
Eco47I GGWCC 2 cut(s) 17, 137
EcoO109I RGGNCCY 2 cut(s) 17, 137
FaeI CATG 1 cut(s) 337
FaiI YATR 7 cut(s) 69, 105, 122, 236, 335, 342, 369
FaqI GGGAC 1 cut(s) 3
FatI CATG 1 cut(s) 333
FauI CCCGC 1 cut(s) 7
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 331
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 1 cut(s) 59
GluI GCNGC 1 cut(s) 331
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 211
Hin1II CATG 1 cut(s) 337
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HinfI GANTC 2 cut(s) 256, 311
HpaII CCGG 1 cut(s) 265
Hpy166II GTNNAC 3 cut(s) 54, 209, 364
Hpy188I TCNGA 4 cut(s) 10, 41, 169, 316
Hpy8I GTNNAC 3 cut(s) 54, 209, 364
Hpy99I CGWCG 3 cut(s) 210, 213, 327
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 3 cut(s) 181, 290, 301
HpyCH4IV ACGT 2 cut(s) 211, 238
HpyCH4V TGCA 2 cut(s) 333, 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 181
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 2 cut(s) 211, 238
Hsp92I GRCGYC 1 cut(s) 211
Hsp92II CATG 1 cut(s) 337
KflI GGGWCCC 1 cut(s) 17
Kzo9I GATC 1 cut(s) 227
LpnPI CCDG 2 cut(s) 185, 278
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 59
MaeII ACGT 2 cut(s) 211, 238
MaeIII GTNAC 1 cut(s) 308
MalI GATC 1 cut(s) 229
MboI GATC 1 cut(s) 227
MboII GAAGA 2 cut(s) 206, 285
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 2 cut(s) 78, 345
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 192, 309
MnlI CCTC 8 cut(s) 4, 91, 121, 150, 175, 213, 240, 330
MseI TTAA 1 cut(s) 77
MspI CCGG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 163
MwoI GCNNNNNNNGC 2 cut(s) 68, 181
NdeII GATC 1 cut(s) 227
NlaIII CATG 1 cut(s) 337
NlaIV GGNNCC 3 cut(s) 18, 19, 138
NmuCI GTSAC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 2 cut(s) 205, 214
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PpuMI RGGWCCY 2 cut(s) 17, 137
Psp5II RGGWCCY 2 cut(s) 17, 137
PspN4I GGNNCC 3 cut(s) 18, 19, 138
PspPI GGNCC 2 cut(s) 17, 137
PspPPI RGGWCCY 2 cut(s) 17, 137
SalI GTCGAC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 77
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 2 cut(s) 17, 137
SchI GAGTC 1 cut(s) 305
SduI GDGCHC 1 cut(s) 366
SetI ASST 8 cut(s) 102, 152, 167, 199, 214, 241, 274, 341
SfaNI GCATC 1 cut(s) 328
SgrDI CGTCGACG 1 cut(s) 207
SinI GGWCC 2 cut(s) 17, 137
Sse9I AATT 2 cut(s) 78, 345
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 59
TaaI ACNGT 3 cut(s) 181, 290, 301
TaiI ACGT 2 cut(s) 214, 241
TaqI TCGA 3 cut(s) 208, 259, 296
TasI AATT 2 cut(s) 78, 345
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 1 cut(s) 330
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 2 cut(s) 17, 170
TspRI CASTG 1 cut(s) 186
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 17, 137
XmiI GTMKAC 1 cut(s) 208
XspI CTAG 1 cut(s) 59
ZraI GACGTC 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.