pycom08g19740

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000800
Physical Location & Seq
Forward (+)
19720275 .. 19723822
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19740.1

Sequence Viewer

Length: 1107 bp
ATGATATGTAGTTTACCGATTGGTGTACCTGATCTGAAATGTCTCAGGGTATTAGATGCAAGCACATGTGAGAAGTTGTACAGGAATCTCGTTGTCTACCTGAGTAGGTGTACTCAATTTTTAGTTGGGGAGCTATTATCGTTCAATGGGGATCTTGTGCAAAAGTTCTGCCTTGTAACCATGGCTGAGTGTGTCAAGTCATCAATAAAAGATACAATCTTCAAGTTTGCCCGTACGATGTGTTCATCTTTGTTCTTGTCTCGAGATGACAGATTCACGCTCTCTCGCATCTTATTTTGCTTGTTGGACTCCCTTGTCCACGAATGTGAGGTTGGAGCACCTTTTCATTTGATTCTGAGGGTTTATGCCACTGGATTGCACTTTATTGATCGCAGTATGAAGTCAAAAATTAGTCATCTTCAATCATTTGAAGGTGCAGTTAGAGTTTTGTTTGATGATGGGGACGCAATGAATCGTTTGTCCGATTTGCTTGGTTCATGGAGAAGTTTTTTCCAATTTGACTGCAGACGACCCTTTGTTATCCAATTCACAGACAGAAAGAACTATCTTCTGTCTTATTTTAATGCATTGAAATTCTTATGCCGGCCGCTTACAGAGGTAGTAAATTCAGGAAGGAAACAGATTATTACAAATAATGAAGCTGCTTCTGTTTCTACTGAATTGTGCCATATCCAGGGTTGTCATTCTTATGATATAATACTGAAATGGTCCGAGTTATTTGTAATTTGCTTGCCTTTTTCTTATTTCACAAGAGTGCAAATACTTGAAAATATCATCACCAGTGCTTGGATTCAACCTCATGGTCTAAAGCATCCATATGTCTCTCTGTACAATACCGGGATACATTTGTACCGAAACAAGGAGCTAAAAGAGGCATCAGAGGGATTGAACTTACGTTGTAAGGCATCATGGACTCGTGTTATACGTCTTTGTGAGATGTTTGCACACAAACAGAAGGTGTCCCAGGTTGATCTGTCGGAAGATGCCATTGTAGATTTCTTTAATGAGTGCTGTACGAGAAGTGCTTTTCCTTTGGATGTTCTTAATGACTTGCACAGTTATGATGTGGAAAGAACATTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

369

Amino Acids

42.41

Weight (kDa)

8.06

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 14 - 84 1e-23 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 55 - 215 1.5e-20 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 807
AccI GTMKAC 1 cut(s) 96
AciI CCGC 1 cut(s) 608
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 605
AcsI RAATTY 2 cut(s) 593, 625
AfaI GTAC 7 cut(s) 27, 80, 112, 235, 851, 872, 1036
AfiI CCNNNNNNNGG 3 cut(s) 694, 807, 880
AflIII ACRYGT 1 cut(s) 65
AgsI TTSAA 8 cut(s) 145, 223, 422, 431, 592, 788, 815, 910
AjnI CCWGG 2 cut(s) 693, 984
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AleI CACNNNNGTG 2 cut(s) 324, 773
AluBI AGCT 3 cut(s) 133, 662, 886
AluI AGCT 3 cut(s) 133, 662, 886
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 3 cut(s) 47, 264, 847
AlwI GGATC 1 cut(s) 159
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 1 cut(s) 662
ApoI RAATTY 2 cut(s) 593, 625
AspS9I GGNCC 1 cut(s) 729
AsuC2I CCSGG 1 cut(s) 859
AsuHPI GGTGA 1 cut(s) 790
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 729
BaeI ACNNNNGTAYC 4 cut(s) 854, 854, 887, 887
BauI CACGAG 1 cut(s) 936
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 1 cut(s) 649
BccI CCATC 1 cut(s) 452
BciT130I CCWGG 2 cut(s) 695, 986
BciVI GTATCC 1 cut(s) 855
BcnI CCSGG 1 cut(s) 859
BcoDI GTCTC 3 cut(s) 47, 264, 847
BfmI CTRYAG 1 cut(s) 523
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 2 cut(s) 608, 663
BlsI GCNGC 2 cut(s) 609, 664
Bme1390I CCNGG 3 cut(s) 695, 859, 986
Bme18I GGWCC 1 cut(s) 729
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 729
BmrFI CCNGG 3 cut(s) 695, 859, 986
BmsI GCATC 6 cut(s) 46, 297, 841, 905, 935, 994
BpuMI CCSGG 1 cut(s) 859
BsaJI CCNNGG 3 cut(s) 180, 694, 984
Bsc4I CCNNNNNNNGG 3 cut(s) 694, 807, 880
Bse118I RCCGGY 1 cut(s) 603
Bse1I ACTGG 2 cut(s) 376, 801
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 2 cut(s) 695, 986
BseDI CCNNGG 3 cut(s) 180, 694, 984
BseGI GGATG 2 cut(s) 832, 1063
BseLI CCNNNNNNNGG 3 cut(s) 694, 807, 880
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 4 cut(s) 58, 92, 177, 347
BseNI ACTGG 2 cut(s) 376, 801
BseX3I CGGCCG 1 cut(s) 605
BseXI GCAGC 1 cut(s) 649
BsgI GTGCAG 1 cut(s) 456
Bsh1285I CGRYCG 1 cut(s) 608
BshFI GGCC 1 cut(s) 607
BsiEI CGRYCG 1 cut(s) 608
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 604, 858
BsiWI CGTACG 1 cut(s) 233
BslFI GGGAC 2 cut(s) 476, 967
BslI CCNNNNNNNGG 3 cut(s) 694, 807, 880
BsmAI GTCTC 3 cut(s) 47, 264, 847
BsmFI GGGAC 2 cut(s) 476, 967
BsnI GGCC 1 cut(s) 607
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 1 cut(s) 340
Bsp1407I TGTACA 2 cut(s) 78, 849
Bsp143I GATC 4 cut(s) 31, 151, 388, 991
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 1 cut(s) 608
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 4 cut(s) 57, 93, 178, 348
BspMAI CTGCAG 1 cut(s) 527
BspPI GGATC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 603
BsrGI TGTACA 2 cut(s) 78, 849
BsrI ACTGG 2 cut(s) 376, 801
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 3 cut(s) 180, 694, 984
BssMI GATC 4 cut(s) 31, 151, 388, 991
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 2 cut(s) 695, 986
Bst4CI ACNGT 1 cut(s) 1079
BstAUI TGTACA 2 cut(s) 78, 849
BstC8I GCNNGC 3 cut(s) 61, 605, 752
BstDEI CTNAG 4 cut(s) 44, 101, 186, 356
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 832, 1063
BstKTI GATC 4 cut(s) 34, 154, 391, 994
BstMAI GTCTC 3 cut(s) 47, 264, 847
BstMBI GATC 4 cut(s) 31, 151, 388, 991
BstMCI CGRYCG 1 cut(s) 608
BstNI CCWGG 2 cut(s) 695, 986
BstNSI RCATGY 1 cut(s) 69
BstSCI CCNGG 3 cut(s) 693, 857, 984
BstSFI CTRYAG 1 cut(s) 523
BstV1I GCAGC 1 cut(s) 649
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BstZI CGGCCG 1 cut(s) 605
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 1 cut(s) 607
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 2 cut(s) 832, 1063
BtsIMutI CAGTG 2 cut(s) 369, 808
Cac8I GCNNGC 3 cut(s) 61, 605, 752
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 1 cut(s) 729
CseI GACGC 1 cut(s) 473
Csp6I GTAC 7 cut(s) 26, 79, 111, 234, 850, 871, 1035
CviAII CATG 5 cut(s) 66, 181, 498, 821, 930
CviJI RGCY 5 cut(s) 133, 185, 607, 662, 886
CviKI_1 RGCY 5 cut(s) 133, 185, 607, 662, 886
CviQI GTAC 7 cut(s) 26, 79, 111, 234, 850, 871, 1035
DdeI CTNAG 4 cut(s) 44, 101, 186, 356
DpnI GATC 4 cut(s) 33, 153, 390, 993
DpnII GATC 4 cut(s) 31, 151, 388, 991
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 605
EagI CGGCCG 1 cut(s) 605
EclXI CGGCCG 1 cut(s) 605
Eco130I CCWWGG 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 729
Eco52I CGGCCG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 693, 984
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 5 cut(s) 69, 184, 501, 824, 933
FaqI GGGAC 2 cut(s) 476, 967
FatI CATG 5 cut(s) 65, 180, 497, 820, 929
FauNDI CATATG 1 cut(s) 838
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 608, 663
FokI GGATG 2 cut(s) 819, 1070
Fsp4HI GCNGC 2 cut(s) 608, 663
GluI GCNGC 2 cut(s) 608, 663
HaeIII GGCC 1 cut(s) 607
HapII CCGG 2 cut(s) 604, 858
HgaI GACGC 1 cut(s) 473
Hin1II CATG 5 cut(s) 69, 184, 501, 824, 933
HinfI GANTC 7 cut(s) 85, 273, 308, 352, 472, 811, 934
HpaII CCGG 2 cut(s) 604, 858
HphI GGTGA 1 cut(s) 790
Hpy166II GTNNAC 5 cut(s) 14, 26, 97, 111, 319
Hpy188I TCNGA 6 cut(s) 36, 357, 484, 733, 901, 1000
Hpy188III TCNNGA 3 cut(s) 261, 263, 630
Hpy8I GTNNAC 5 cut(s) 14, 26, 97, 111, 319
HpyAV CCTTC 3 cut(s) 425, 627, 970
HpyCH4III ACNGT 1 cut(s) 1079
HpyCH4IV ACGT 2 cut(s) 916, 946
HpyCH4V TGCA 9 cut(s) 59, 160, 379, 437, 525, 587, 778, 965, 1075
HpyF3I CTNAG 4 cut(s) 44, 101, 186, 356
HpySE526I ACGT 2 cut(s) 916, 946
Hsp92II CATG 5 cut(s) 69, 184, 501, 824, 933
KroI GCCGGC 1 cut(s) 603
KroNI GCCGGC 1 cut(s) 605
Kzo9I GATC 4 cut(s) 31, 151, 388, 991
LmnI GCTCC 3 cut(s) 130, 335, 883
Lsp1109I GCAGC 1 cut(s) 649
LweI GCATC 6 cut(s) 46, 297, 841, 905, 935, 994
MaeII ACGT 2 cut(s) 916, 946
MaeIII GTNAC 1 cut(s) 175
MalI GATC 4 cut(s) 33, 153, 390, 993
MboI GATC 4 cut(s) 31, 151, 388, 991
MboII GAAGA 4 cut(s) 211, 410, 560, 1013
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 8 cut(s) 116, 408, 515, 545, 593, 625, 680, 744
MlyI GAGTC 2 cut(s) 302, 928
MmeI TCCRAC 3 cut(s) 285, 313, 978
MnlI CCTC 6 cut(s) 322, 351, 610, 828, 886, 895
Mph1103I ATGCAT 1 cut(s) 589
MroNI GCCGGC 1 cut(s) 603
MseI TTAA 3 cut(s) 582, 1023, 1065
MslI CAYNNNNRTG 5 cut(s) 324, 708, 773, 837, 1080
MspI CCGG 2 cut(s) 604, 858
MspR9I CCNGG 3 cut(s) 695, 859, 986
MvaI CCWGG 2 cut(s) 695, 986
NaeI GCCGGC 1 cut(s) 605
NciI CCSGG 1 cut(s) 859
NcoI CCATGG 1 cut(s) 180
NdeI CATATG 1 cut(s) 838
NdeII GATC 4 cut(s) 31, 151, 388, 991
NgoMIV GCCGGC 1 cut(s) 603
NlaIII CATG 5 cut(s) 69, 184, 501, 824, 933
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 69
OliI CACNNNNGTG 2 cut(s) 324, 773
PaeR7I CTCGAG 1 cut(s) 261
PciI ACATGT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 943
PdiI GCCGGC 1 cut(s) 605
PfeI GAWTC 5 cut(s) 85, 273, 352, 472, 811
Pfl23II CGTACG 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 807
PkrI GCNGC 2 cut(s) 609, 664
PleI GAGTC 2 cut(s) 302, 928
PpsI GAGTC 2 cut(s) 302, 928
PscI ACATGT 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 693, 984
PspGI CCWGG 2 cut(s) 693, 984
PspLI CGTACG 1 cut(s) 233
PspPI GGNCC 1 cut(s) 729
PsrI GAACNNNNNNTAC 2 cut(s) 226, 258
PstI CTGCAG 1 cut(s) 527
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 7 cut(s) 27, 80, 112, 235, 851, 872, 1036
RsaNI GTAC 7 cut(s) 26, 79, 111, 234, 850, 871, 1035
RseI CAYNNNNRTG 5 cut(s) 324, 708, 773, 837, 1080
SaqAI TTAA 3 cut(s) 582, 1023, 1065
SatI GCNGC 2 cut(s) 608, 663
Sau3AI GATC 4 cut(s) 31, 151, 388, 991
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 2 cut(s) 302, 928
ScrFI CCNGG 3 cut(s) 695, 859, 986
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 6 cut(s) 46, 297, 841, 905, 935, 994
SfcI CTRYAG 1 cut(s) 523
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 729
SlaI CTCGAG 1 cut(s) 261
SmiMI CAYNNNNRTG 5 cut(s) 324, 708, 773, 837, 1080
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 8 cut(s) 116, 408, 515, 545, 593, 625, 680, 744
SsiI CCGC 1 cut(s) 608
StyD4I CCNGG 3 cut(s) 693, 857, 984
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 1079
TaiI ACGT 2 cut(s) 919, 949
TaqI TCGA 1 cut(s) 262
TasI AATT 8 cut(s) 116, 408, 515, 545, 593, 625, 680, 744
TatI WGTACW 3 cut(s) 78, 110, 849
TauI GCSGC 1 cut(s) 610
TfiI GAWTC 5 cut(s) 85, 273, 352, 472, 811
Tru1I TTAA 3 cut(s) 582, 1023, 1065
Tru9I TTAA 3 cut(s) 582, 1023, 1065
TscAI CASTG 2 cut(s) 376, 808
TseI GCWGC 1 cut(s) 662
TspDTI ATGAA 6 cut(s) 234, 335, 413, 485, 486, 672
TspRI CASTG 2 cut(s) 376, 808
Van91I CCANNNNNTGG 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 729
XapI RAATTY 2 cut(s) 593, 625
XceI RCATGY 1 cut(s) 69
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.