Rh7BG010300

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000800
Physical Location & Seq
Forward (+)
655349 .. 661459
6111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG010300.1

Sequence Viewer

Length: 2145 bp
ATGTGTTTTGGATCATTGGATGTAATTGATTGGTTGAAATATGACAGGGTATTGGATAAACATCTCCGCCAGCTCGTGCTCGAGGAGCAAGCGAGCTCTCTTATGACTTGTACCATCTTTTTAGTTGGCGAGGTTGATCAATTTGATCTTGCAAGCAAGTTTTGTGACCTAATTATCACCACATCAGTCCAAGGATCTCCTCACCTAACGAAGCTATGTGATTCCCTTTATCAAATTGTGTCTCTCCAGGCTAATTCAGAAGCTTTGAAGTTGCATTGTCAGGCAACATGGGTTATAATCGAAGGAAAACTAAAGGAGGCAGAAACTAGATTATCAAATTATGACAGCTTGGAATTATTTGGCAAGTGGTGTGAAGCAAGTACTTTACTACTCCAAGTTCTTAGTCAATCTGAGGGTACTCATGAAGTGCGAGTAAAACTCGTAAAAGGTCATGAAAATGGGGATGATGTGGGCACTTTGTTAGGCAATTTGTTTCTATCTTCTAGCAAAGTATCAAGAAAGAAGATCTTGCAGCAGAGGTCCAAAATACTACACTCAATGTCTCAATGCTTGTCACAAGCACTTAAAGTTGCTTCTGGCCATCAATTAGCTGTGGCATATTGTTTACGATCACTTTGCACCGAGGAATATGAACCAAACTCAGAGAAAGTGTTTCAAGATATTCAGTCTGCCCTTGTTGTGTGGTCGAGCATTCCTACTTCAGATGGCTCCATGGTGACTGAAAATATAATGAAGATACTTTATTCTGTAATTGATTTGTTATCCCTCAAGGGTCGCGTAGACTTTCACTGTACTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAGCCTGTCACATGTTTTTGGCGAAAAATTAAAGTTAACTGAGTTTTGGATAGAGTGTTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATTTCTCATCTTCTCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTTTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGCTGGATATATGCGCTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAATTGAGGCTCTTCCATATGCAATGGAAGCCCACCGCTTACGAGTAGAACTATTCAAACAGAAATTTCCATGGTCTTTTGAAGGACGCGGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCCTTTGATACTAATTCATGTGACTTGGACATCTGCTATATTACCCCCTGGAATGCACTTCAGTGCTATCTTGAGAGCATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTGAGGCCGAATATTTTTTGGATCGGGGAAAAAGTCTTTCCTGCTCACATAGCTTGACTCTTTTTAACATTGTTTTTTCTTCTTTTTTGGGAGAACTATACCATAAGAAGCAGCGTTGGGCTTCAGCAAAAAAGGAACTTCAAAGTTCCAGGGAACTCTTGAAGGATAGCATAATAGATGGTTTTTGCTCAAAATGCAAAGTGATACTTGAAGTAACTGTTACACATAATCTTGCTGATTTATACCAAAGCCCCATCAGTGAAATTGAGAGTGCATCTGCAGAGAAATTATCTACTGATCTCTACAAAGAAACTCTAGCCAAGCTACATACTTCAGAGTGGAAAAAGTCTATTGCTTGTCTTGAAGAACAAGATGGTACTATTTTAGTCAAAGATGAATCATGGTTTGGCAGTACTTTGAAATATGTGCTTCTTGGTGAAAGTGTCCAACTGCAAGGACCGTTCTGTGGGAACCTTGTGAGGAAATTGAAATCTGAATTCACGGACATAGATTCTAGTCTTCTGAAAGTCATCGTCCTTGGGGTGTCCAAATATGGCTTTAAAGTAGGCACAGAACAAGTTATGTTGGAGTTTTGCCTCAATAAAGGTTCTTTAGTGATGTTTAATTGTCACCAAAATAAGGCCAATTGGATTTTTTCATATGAAGTGGATCGAGCTACCAGGATAACAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

714

Amino Acids

80.39

Weight (kDa)

6.09

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 296
AccB7I CCANNNNNTGG 2 cut(s) 885, 1021
AccI GTMKAC 1 cut(s) 801
AccII CGCG 2 cut(s) 798, 1278
AciI CCGC 3 cut(s) 67, 1225, 1278
AclWI GGATC 5 cut(s) 19, 202, 908, 1479, 2127
AcoI YGGCCR 2 cut(s) 598, 876
AcsI RAATTY 2 cut(s) 1253, 1946
AcuI CTGAAG 5 cut(s) 705, 1373, 1397, 1557, 1767
AcvI CACGTG 1 cut(s) 1294
AfaI GTAC 6 cut(s) 112, 382, 418, 814, 1828, 1864
AfiI CCNNNNNNNGG 4 cut(s) 885, 1021, 1916, 2089
AflIII ACRYGT 1 cut(s) 955
AjnI CCWGG 4 cut(s) 246, 1376, 1598, 2129
AjuI GAANNNNNNNTTGG 4 cut(s) 857, 889, 1839, 1871
AleI CACNNNNGTG 1 cut(s) 1390
Alw21I GWGCWC 2 cut(s) 81, 98
Alw26I GTCTC 3 cut(s) 246, 567, 862
AlwI GGATC 5 cut(s) 19, 202, 908, 1479, 2127
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 4 cut(s) 598, 876, 1455, 2091
ApeKI GCWGC 2 cut(s) 532, 1561
ApoI RAATTY 2 cut(s) 1253, 1946
AspLEI GCGC 1 cut(s) 1152
AspS9I GGNCC 3 cut(s) 540, 1329, 1907
AsuHPI GGTGA 6 cut(s) 169, 194, 748, 1292, 1898, 2072
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 3 cut(s) 540, 1329, 1907
BaeGI GKGCMC 1 cut(s) 476
BalI TGGCCA 2 cut(s) 600, 878
BanII GRGCYC 1 cut(s) 98
BauI CACGAG 1 cut(s) 74
BbrPI CACGTG 1 cut(s) 1294
BbsI GAAGAC 1 cut(s) 1961
Bbv12I GWGCWC 2 cut(s) 81, 98
BbvCI CCTCAGC 1 cut(s) 1452
BbvI GCAGC 2 cut(s) 544, 1573
BccI CCATC 6 cut(s) 122, 609, 719, 1622, 1712, 1817
BcgI CGANNNNNNTGC 2 cut(s) 618, 652
BciT130I CCWGG 4 cut(s) 248, 1378, 1600, 2131
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 246, 567, 862
BfaI CTAG 7 cut(s) 327, 504, 1136, 1298, 1317, 1766, 1965
BfmI CTRYAG 2 cut(s) 1303, 1728
BglII AGATCT 2 cut(s) 525, 837
BisI GCNGC 2 cut(s) 533, 1562
BlsI GCNGC 2 cut(s) 534, 1563
BmcAI AGTACT 2 cut(s) 382, 1864
Bme1390I CCNGG 4 cut(s) 248, 1378, 1600, 2131
Bme18I GGWCC 3 cut(s) 540, 1329, 1907
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 3 cut(s) 540, 1329, 1907
BmiI GGNNCC 2 cut(s) 730, 1922
BmrFI CCNGG 4 cut(s) 248, 1378, 1600, 2131
BmrI ACTGGG 1 cut(s) 1448
BmsI GCATC 1 cut(s) 1733
BmuI ACTGGG 1 cut(s) 1448
BpiI GAAGAC 1 cut(s) 1961
BplI GAGNNNNNCTC 2 cut(s) 423, 455
BpmI CTGGAG 2 cut(s) 230, 909
Bpu10I CCTNAGC 1 cut(s) 1452
BpuEI CTTGAG 2 cut(s) 773, 1421
BsaAI YACGTR 1 cut(s) 1294
BsaBI GATNNNNATC 2 cut(s) 60, 836
BsaJI CCNNGG 7 cut(s) 190, 642, 732, 1259, 1376, 1599, 1987
Bsc4I CCNNNNNNNGG 4 cut(s) 885, 1021, 1916, 2089
Bse1I ACTGG 2 cut(s) 926, 1443
Bse3DI GCAATG 1 cut(s) 1218
Bse8I GATNNNNATC 2 cut(s) 60, 836
BseBI CCWGG 4 cut(s) 248, 1378, 1600, 2131
BseDI CCNNGG 7 cut(s) 190, 642, 732, 1259, 1376, 1599, 1987
BseGI GGATG 2 cut(s) 25, 469
BseJI GATNNNNATC 2 cut(s) 60, 836
BseLI CCNNNNNNNGG 4 cut(s) 885, 1021, 1916, 2089
BseMI GCAATG 1 cut(s) 1218
BseMII CTCAG 4 cut(s) 402, 675, 975, 1443
BseNI ACTGG 2 cut(s) 926, 1443
BseRI GAGGAG 3 cut(s) 98, 189, 867
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 2 cut(s) 544, 1573
BseYI CCCAGC 1 cut(s) 1014
Bsh1236I CGCG 2 cut(s) 798, 1278
BshFI GGCC 4 cut(s) 600, 878, 1457, 2093
BsiHKAI GWGCWC 2 cut(s) 81, 98
BsiHKCI CYCGRG 1 cut(s) 80
BslI CCNNNNNNNGG 4 cut(s) 885, 1021, 1916, 2089
BsmAI GTCTC 3 cut(s) 246, 567, 862
BsmI GAATGC 3 cut(s) 711, 1387, 1407
BsnI GGCC 4 cut(s) 600, 878, 1457, 2093
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 81, 98, 476
Bsp19I CCATGG 2 cut(s) 732, 1259
BspACI CCGC 3 cut(s) 67, 1225, 1278
BspANI GGCC 4 cut(s) 600, 878, 1457, 2093
BspCNI CTCAG 4 cut(s) 403, 674, 976, 1444
BspFNI CGCG 2 cut(s) 798, 1278
BspHI TCATGA 2 cut(s) 421, 451
BspLI GGNNCC 2 cut(s) 730, 1922
BspMAI CTGCAG 1 cut(s) 1732
BspPI GGATC 5 cut(s) 19, 202, 908, 1479, 2127
BspQI GCTCTTC 1 cut(s) 1206
BsrDI GCAATG 1 cut(s) 1218
BsrI ACTGG 2 cut(s) 926, 1443
BssECI CCNNGG 7 cut(s) 190, 642, 732, 1259, 1376, 1599, 1987
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 4 cut(s) 190, 732, 1259, 1987
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 4 cut(s) 248, 1378, 1600, 2131
Bst4CI ACNGT 4 cut(s) 812, 1072, 1669, 1911
Bst6I CTCTTC 1 cut(s) 1206
BstBAI YACGTR 1 cut(s) 1294
BstC8I GCNNGC 4 cut(s) 71, 90, 94, 154
BstDEI CTNAG 5 cut(s) 401, 411, 661, 984, 1452
BstDSI CCRYGG 2 cut(s) 732, 1259
BstF5I GGATG 2 cut(s) 25, 469
BstFNI CGCG 2 cut(s) 798, 1278
BstHHI GCGC 1 cut(s) 1152
BstMAI GTCTC 3 cut(s) 246, 567, 862
BstMWI GCNNNNNNNGC 4 cut(s) 85, 1217, 1500, 1644
BstNI CCWGG 4 cut(s) 248, 1378, 1600, 2131
BstNSI RCATGY 1 cut(s) 959
BstSCI CCNGG 4 cut(s) 246, 1376, 1598, 2129
BstSFI CTRYAG 2 cut(s) 1303, 1728
BstSLI GKGCMC 1 cut(s) 476
BstUI CGCG 2 cut(s) 798, 1278
BstV1I GCAGC 2 cut(s) 544, 1573
BstV2I GAAGAC 1 cut(s) 1961
BstX2I RGATCY 3 cut(s) 194, 525, 837
BstYI RGATCY 3 cut(s) 194, 525, 837
BsuRI GGCC 4 cut(s) 600, 878, 1457, 2093
BtgI CCRYGG 2 cut(s) 732, 1259
BtsCI GGATG 2 cut(s) 25, 469
BtsIMutI CAGTG 3 cut(s) 808, 1397, 1714
Cac8I GCNNGC 4 cut(s) 71, 90, 94, 154
CciI TCATGA 2 cut(s) 421, 451
CfoI GCGC 1 cut(s) 1152
Cfr13I GGNCC 3 cut(s) 540, 1329, 1907
CseI GACGC 1 cut(s) 1284
Csp6I GTAC 6 cut(s) 111, 381, 417, 813, 1827, 1863
CviQI GTAC 6 cut(s) 111, 381, 417, 813, 1827, 1863
DdeI CTNAG 5 cut(s) 401, 411, 661, 984, 1452
DraI TTTAAA 1 cut(s) 2011
EaeI YGGCCR 2 cut(s) 598, 876
Eam1104I CTCTTC 1 cut(s) 1206
EarI CTCTTC 1 cut(s) 1206
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 4 cut(s) 190, 732, 1259, 1987
Eco24I GRGCYC 1 cut(s) 98
Eco47I GGWCC 3 cut(s) 540, 1329, 1907
Eco53kI GAGCTC 1 cut(s) 96
Eco57I CTGAAG 5 cut(s) 705, 1373, 1397, 1557, 1767
Eco72I CACGTG 1 cut(s) 1294
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 96
EcoRI GAATTC 1 cut(s) 1946
EcoRII CCWGG 4 cut(s) 246, 1376, 1598, 2129
EcoT14I CCWWGG 4 cut(s) 190, 732, 1259, 1987
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 4 cut(s) 190, 732, 1259, 1987
FalI AAGNNNNNCTT 4 cut(s) 512, 544, 1641, 1673
FauNDI CATATG 2 cut(s) 1207, 2110
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 2 cut(s) 533, 1562
FokI GGATG 2 cut(s) 32, 476
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 2 cut(s) 533, 1562
FspBI CTAG 7 cut(s) 327, 504, 1136, 1298, 1317, 1766, 1965
GlaI GCGC 1 cut(s) 1151
GluI GCNGC 2 cut(s) 533, 1562
GsaI CCCAGC 1 cut(s) 1018
GsuI CTGGAG 2 cut(s) 230, 909
HaeIII GGCC 4 cut(s) 600, 878, 1457, 2093
HgaI GACGC 1 cut(s) 1284
HhaI GCGC 1 cut(s) 1152
Hin6I GCGC 1 cut(s) 1150
HinP1I GCGC 1 cut(s) 1150
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 8 cut(s) 221, 855, 890, 1121, 1288, 1507, 1847, 1961
HpaI GTTAAC 1 cut(s) 981
HphI GGTGA 6 cut(s) 169, 194, 748, 1292, 1898, 2072
Hpy166II GTNNAC 4 cut(s) 626, 802, 981, 1322
Hpy188I TCNGA 8 cut(s) 259, 412, 664, 724, 1120, 1786, 1945, 1974
Hpy188III TCNNGA 9 cut(s) 422, 452, 516, 677, 894, 932, 1400, 1609, 1811
Hpy8I GTNNAC 4 cut(s) 626, 802, 981, 1322
HpyAV CCTTC 6 cut(s) 296, 891, 930, 1265, 1277, 1606
HpyCH4III ACNGT 4 cut(s) 812, 1072, 1669, 1911
HpyCH4IV ACGT 1 cut(s) 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 1217, 1500, 1644
HpyF3I CTNAG 5 cut(s) 401, 411, 661, 984, 1452
HpySE526I ACGT 1 cut(s) 1293
HspAI GCGC 1 cut(s) 1150
Ksp22I TGATCA 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 981
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 3 cut(s) 85, 734, 1448
Lsp1109I GCAGC 2 cut(s) 544, 1573
LweI GCATC 1 cut(s) 1733
MaeI CTAG 7 cut(s) 327, 504, 1136, 1298, 1317, 1766, 1965
MaeII ACGT 1 cut(s) 1293
MaeIII GTNAC 9 cut(s) 164, 573, 736, 951, 1090, 1349, 1663, 1669, 2078
MfeI CAATTG 2 cut(s) 1191, 2095
MflI RGATCY 3 cut(s) 194, 525, 837
MhlI GDGCHC 3 cut(s) 81, 98, 476
MlsI TGGCCA 2 cut(s) 600, 878
MluNI TGGCCA 2 cut(s) 600, 878
MlyI GAGTC 2 cut(s) 864, 1501
MmeI TCCRAC 2 cut(s) 1921, 2016
Mox20I TGGCCA 2 cut(s) 600, 878
MscI TGGCCA 2 cut(s) 600, 878
MseI TTAA 7 cut(s) 585, 834, 974, 980, 1515, 2010, 2073
MslI CAYNNNNRTG 2 cut(s) 456, 1390
Msp20I TGGCCA 2 cut(s) 600, 878
MspR9I CCNGG 4 cut(s) 248, 1378, 1600, 2131
MunI CAATTG 2 cut(s) 1191, 2095
Mva1269I GAATGC 3 cut(s) 711, 1387, 1407
MvaI CCWGG 4 cut(s) 248, 1378, 1600, 2131
MvnI CGCG 2 cut(s) 798, 1278
MwoI GCNNNNNNNGC 4 cut(s) 85, 1217, 1500, 1644
NcoI CCATGG 2 cut(s) 732, 1259
NdeI CATATG 2 cut(s) 1207, 2110
NlaIV GGNNCC 2 cut(s) 730, 1922
NmuCI GTSAC 6 cut(s) 164, 573, 736, 951, 1349, 2078
NspI RCATGY 1 cut(s) 959
OliI CACNNNNGTG 1 cut(s) 1390
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 2 cut(s) 421, 451
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 1206
PctI GAATGC 3 cut(s) 711, 1387, 1407
PfeI GAWTC 6 cut(s) 221, 890, 1121, 1288, 1847, 1961
PflMI CCANNNNNTGG 2 cut(s) 885, 1021
PkrI GCNGC 2 cut(s) 534, 1563
PleI GAGTC 2 cut(s) 863, 1501
PmaCI CACGTG 1 cut(s) 1294
PmlI CACGTG 1 cut(s) 1294
PpsI GAGTC 2 cut(s) 863, 1501
Ppu21I YACGTR 1 cut(s) 1294
PscI ACATGT 1 cut(s) 955
PsiI TTATAA 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 4 cut(s) 246, 1376, 1598, 2129
PspCI CACGTG 1 cut(s) 1294
PspFI CCCAGC 1 cut(s) 1014
PspGI CCWGG 4 cut(s) 246, 1376, 1598, 2129
PspN4I GGNNCC 2 cut(s) 730, 1922
PspPI GGNCC 3 cut(s) 540, 1329, 1907
PspXI VCTCGAGB 1 cut(s) 80
PstI CTGCAG 1 cut(s) 1732
PsuI RGATCY 3 cut(s) 194, 525, 837
RsaI GTAC 6 cut(s) 112, 382, 418, 814, 1828, 1864
RsaNI GTAC 6 cut(s) 111, 381, 417, 813, 1827, 1863
RseI CAYNNNNRTG 2 cut(s) 456, 1390
SacI GAGCTC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 7 cut(s) 585, 834, 974, 980, 1515, 2010, 2073
SatI GCNGC 2 cut(s) 533, 1562
Sau96I GGNCC 3 cut(s) 540, 1329, 1907
ScaI AGTACT 2 cut(s) 382, 1864
SchI GAGTC 2 cut(s) 864, 1501
ScrFI CCNGG 4 cut(s) 248, 1378, 1600, 2131
SduI GDGCHC 3 cut(s) 81, 98, 476
SfaNI GCATC 1 cut(s) 1733
SfcI CTRYAG 2 cut(s) 1303, 1728
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 3 cut(s) 540, 1329, 1907
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 456, 1390
SmlI CTYRAG 3 cut(s) 80, 788, 1400
SmoI CTYRAG 3 cut(s) 80, 788, 1400
SsiI CCGC 3 cut(s) 67, 1225, 1278
SspI AATATT 1 cut(s) 1463
SspMI CTAG 7 cut(s) 327, 504, 1136, 1298, 1317, 1766, 1965
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 4 cut(s) 246, 1376, 1598, 2129
StyI CCWWGG 4 cut(s) 190, 732, 1259, 1987
TaaI ACNGT 4 cut(s) 812, 1072, 1669, 1911
TaiI ACGT 1 cut(s) 1296
TaqI TCGA 4 cut(s) 81, 300, 707, 2122
TatI WGTACW 3 cut(s) 380, 812, 1862
TfiI GAWTC 6 cut(s) 221, 890, 1121, 1288, 1847, 1961
Tru1I TTAA 7 cut(s) 585, 834, 974, 980, 1515, 2010, 2073
Tru9I TTAA 7 cut(s) 585, 834, 974, 980, 1515, 2010, 2073
TscAI CASTG 3 cut(s) 815, 1397, 1714
TseFI GTSAC 6 cut(s) 164, 573, 736, 951, 1349, 2078
TseI GCWGC 2 cut(s) 532, 1561
Tsp45I GTSAC 6 cut(s) 164, 573, 736, 951, 1349, 2078
TspGWI ACGGA 1 cut(s) 1967
TspRI CASTG 3 cut(s) 815, 1397, 1714
Van91I CCANNNNNTGG 2 cut(s) 885, 1021
VpaK11BI GGWCC 3 cut(s) 540, 1329, 1907
XapI RAATTY 2 cut(s) 1253, 1946
XceI RCATGY 1 cut(s) 959
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 801
XspI CTAG 7 cut(s) 327, 504, 1136, 1298, 1317, 1766, 1965
ZrmI AGTACT 2 cut(s) 382, 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.