Rh7BG353700

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000800
Physical Location & Seq
Reverse (-)
36982696 .. 36984884
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG353700.1

Sequence Viewer

Length: 543 bp
ATGCTTCTACCAGTGGATTCAGTATTAGAAGGAGATTCAGATGATGTTAGTTATTCTGGAATAAAGGATTGGAACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCAGAGTTTAGATTGATATTGGAGGGGGCACATTCGTCATCTGTGAAACATCCTGTACAAGATACAGATGAGAAAAAGCTTCACTGGTGGGTGGAGAGGAAGAATTTTGATCGCCATGCACTTGGAGGTGCACGTGTTACACAGCTTTGTCTTAAGAAAGGTTCCTTTGTTAGTAAAGTTGGATGTTTAGAGGAAGAGAAGTGTATGGCATCACGTAATGAATCTAATGGTGGCGAGAACTTGTCTGAGTTGGCCAACAAACTAGTATTAGAAGCAGTGAATGGGCTTGAAGGTTTACATTCTTCTGTAAATATAGAGCCAACAATATTAGTTCTGGACTACGAGGTGCAGGTTACACAATCATCTGTTTTGAACTTTTATGTTGTGTACGTGCCGACGGTGCCGTGGTACAGGGCAACAATATATTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

180

Amino Acids

20.04

Weight (kDa)

4.89

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 511
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 1 cut(s) 214
AcvI CACGTG 1 cut(s) 245
AfaI GTAC 3 cut(s) 168, 500, 521
AflII CTTAAG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 2 cut(s) 401, 484
AhlI ACTAGT 1 cut(s) 373
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 3 cut(s) 110, 190, 256
AluI AGCT 3 cut(s) 110, 190, 256
Alw21I GWGCWC 1 cut(s) 244
Alw44I GTGCAC 1 cut(s) 240
AoxI GGCC 1 cut(s) 363
ApaLI GTGCAC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 214
BaeGI GKGCMC 2 cut(s) 142, 244
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 1 cut(s) 511
BbrPI CACGTG 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 244
BceAI ACGGC 1 cut(s) 499
BcuI ACTAGT 1 cut(s) 373
BfaI CTAG 2 cut(s) 374, 541
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 451
BmiI GGNNCC 3 cut(s) 88, 274, 513
BmsI GCATC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 96
BsaAI YACGTR 3 cut(s) 245, 326, 502
BsaJI CCNNGG 2 cut(s) 80, 515
Bse1I ACTGG 2 cut(s) 11, 200
BseDI CCNNGG 2 cut(s) 80, 515
BseGI GGATG 2 cut(s) 160, 299
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 11, 200
BseSI GKGCMC 2 cut(s) 142, 244
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 1 cut(s) 511
BsiHKAI GWGCWC 1 cut(s) 244
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 142, 244
Bsp1407I TGTACA 1 cut(s) 166
Bsp143I GATC 1 cut(s) 220
Bsp19I CCATGG 1 cut(s) 80
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 88, 274, 513
BspMI ACCTGC 1 cut(s) 451
BspT107I GGYRCC 1 cut(s) 511
BspTI CTTAAG 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 166
BsrI ACTGG 2 cut(s) 11, 200
BssECI CCNNGG 2 cut(s) 80, 515
BssMI GATC 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 80
Bst4CI ACNGT 3 cut(s) 77, 94, 511
Bst6I CTCTTC 1 cut(s) 300
BstAFI CTTAAG 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 166
BstBAI YACGTR 3 cut(s) 245, 326, 502
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 2 cut(s) 80, 515
BstF5I GGATG 2 cut(s) 160, 299
BstKTI GATC 1 cut(s) 223
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 511
BstSLI GKGCMC 2 cut(s) 142, 244
BstXI CCANNNNNNTGG 1 cut(s) 233
BsuRI GGCC 1 cut(s) 365
BtgI CCRYGG 2 cut(s) 80, 515
BtsCI GGATG 2 cut(s) 160, 299
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 4 cut(s) 18, 90, 193, 393
BveI ACCTGC 1 cut(s) 451
Csp6I GTAC 3 cut(s) 167, 499, 520
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 2 cut(s) 81, 227
CviJI RGCY 6 cut(s) 110, 190, 256, 365, 397, 430
CviKI_1 RGCY 6 cut(s) 110, 190, 256, 365, 397, 430
CviQI GTAC 3 cut(s) 167, 499, 520
DdeI CTNAG 1 cut(s) 357
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 80
Eco72I CACGTG 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 2 cut(s) 84, 230
FaiI YATR 6 cut(s) 82, 228, 317, 425, 492, 535
FatI CATG 2 cut(s) 80, 226
FokI GGATG 2 cut(s) 147, 306
FspBI CTAG 2 cut(s) 374, 541
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 1 cut(s) 365
Hin1II CATG 2 cut(s) 84, 230
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 3 cut(s) 17, 35, 332
Hpy166II GTNNAC 3 cut(s) 242, 407, 499
Hpy188I TCNGA 2 cut(s) 40, 358
Hpy188III TCNNGA 3 cut(s) 57, 113, 446
Hpy8I GTNNAC 3 cut(s) 242, 407, 499
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 23, 395
HpyCH4III ACNGT 3 cut(s) 77, 94, 511
HpyCH4IV ACGT 3 cut(s) 244, 325, 501
HpyCH4V TGCA 3 cut(s) 230, 242, 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 511
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 3 cut(s) 244, 325, 501
Hsp92II CATG 2 cut(s) 84, 230
Kzo9I GATC 1 cut(s) 220
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 8 cut(s) 24, 42, 126, 177, 181, 431, 446, 508
LweI GCATC 1 cut(s) 329
MaeI CTAG 2 cut(s) 374, 541
MaeII ACGT 3 cut(s) 244, 325, 501
MaeIII GTNAC 2 cut(s) 247, 463
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 3 cut(s) 223, 317, 405
MhlI GDGCHC 2 cut(s) 142, 244
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 1 cut(s) 214
MluNI TGGCCA 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 5 cut(s) 127, 201, 230, 295, 448
Mox20I TGGCCA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 365
MseI TTAA 1 cut(s) 264
Msp20I TGGCCA 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 511
NcoI CCATGG 1 cut(s) 80
NdeII GATC 1 cut(s) 220
NlaIII CATG 2 cut(s) 84, 230
NlaIV GGNNCC 3 cut(s) 88, 274, 513
PfeI GAWTC 3 cut(s) 17, 35, 332
PmaCI CACGTG 1 cut(s) 245
PmlI CACGTG 1 cut(s) 245
Ppu21I YACGTR 3 cut(s) 245, 326, 502
PspCI CACGTG 1 cut(s) 245
PspN4I GGNNCC 3 cut(s) 88, 274, 513
RsaI GTAC 3 cut(s) 168, 500, 521
RsaNI GTAC 3 cut(s) 167, 499, 520
SaqAI TTAA 1 cut(s) 264
Sau3AI GATC 1 cut(s) 220
SduI GDGCHC 2 cut(s) 142, 244
SfaNI GCATC 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SpeI ACTAGT 1 cut(s) 373
Sse9I AATT 1 cut(s) 214
SspI AATATT 1 cut(s) 438
SspMI CTAG 2 cut(s) 374, 541
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 3 cut(s) 77, 94, 511
TaiI ACGT 3 cut(s) 247, 328, 504
TasI AATT 1 cut(s) 214
TatI WGTACW 1 cut(s) 166
TfiI GAWTC 3 cut(s) 17, 35, 332
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 4 cut(s) 18, 97, 200, 393
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 4 cut(s) 18, 97, 200, 393
Vha464I CTTAAG 1 cut(s) 263
VneI GTGCAC 1 cut(s) 240
XapI RAATTY 1 cut(s) 214
XspI CTAG 2 cut(s) 374, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.